{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T05:18:29Z","timestamp":1793423909916,"version":"4.5.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T00:00:00Z","timestamp":1521763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["eBioMedicine"],"published-print":{"date-parts":[[2018,4]]},"DOI":"10.1016\/j.ebiom.2018.03.029","type":"journal-article","created":{"date-parts":[[2018,3,28]],"date-time":"2018-03-28T08:08:14Z","timestamp":1522224494000},"page":"138-147","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":147,"special_numbering":"C","title":["Plasma mSEPT9: A Novel Circulating Cell-free DNA-Based Epigenetic Biomarker to Diagnose Hepatocellular Carcinoma"],"prefix":"10.1016","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9731-4529","authenticated-orcid":false,"given":"Abderrahim","family":"Oussalah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susann","family":"Rischer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mouni","family":"Bensenane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Conroy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Filhine-Tresarrieu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9e","family":"Debard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Forest-Tramoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Josse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Reinicke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Luc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9dric","family":"Baumann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet","family":"Ayav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Laurent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Hollenbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Ripoll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa-Maria","family":"Gu\u00e9ant-Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fares","family":"Namour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Zipprich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Fleischhacker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Bronowicki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Louis","family":"Gu\u00e9ant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ebiom.2018.03.029_bb0005","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/nyas.12529","article-title":"Strategy for prevention of cancers of the esophagus","volume":"1325","author":"Akiyama","year":"2014","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0010","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.cgh.2015.12.042","article-title":"Role of the GALAD and BALAD-2 serologic models in diagnosis of hepatocellular carcinoma and prediction of survival in patients","volume":"14","author":"Berhane","year":"2016","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0015","series-title":"Introduction to Meta-Analysis","first-page":"311","article-title":"Generality of the basic inverse-variance method","author":"Borenstein","year":"2009"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0020","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1002\/hep.24199","article-title":"Management of hepatocellular carcinoma: an update","volume":"53","author":"Bruix","year":"2011","journal-title":"Hepatology"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0025","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1053\/j.gastro.2015.12.041","article-title":"Evidence-based diagnosis, staging, and treatment of patients with hepatocellular carcinoma","volume":"150","author":"Bruix","year":"2016","journal-title":"Gastroenterology"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0030","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1002\/path.1484","article-title":"Altered expression of the septin gene, SEPT9, in ovarian neoplasia","volume":"201","author":"Burrows","year":"2003","journal-title":"J. Pathol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0035","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1136\/gutjnl-2012-304149","article-title":"Prospective evaluation of methylated SEPT9 in plasma for detection of asymptomatic colorectal cancer","volume":"63","author":"Church","year":"2014","journal-title":"Gut"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0040","doi-asserted-by":"crossref","first-page":"837","DOI":"10.2307\/2531595","article-title":"Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach","volume":"44","author":"DeLong","year":"1988","journal-title":"Biometrics"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0045","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0197-2456(86)90046-2","article-title":"Meta-analysis in clinical trials","volume":"7","author":"DerSimonian","year":"1986","journal-title":"Control. Clin. Trials"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0050","series-title":"An Introduction to the Bootstrap","author":"Efron","year":"1994"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0055","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1056\/NEJMra1001683","article-title":"Hepatocellular carcisnoma","volume":"365","author":"El-Serag","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0060","author":"Epigenomics"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0065","first-page":"67","article-title":"Detection of aberrant promoter hypermethylation of tumor suppressor genes in serum DNA from non-small cell lung cancer patients","volume":"59","author":"Esteller","year":"1999","journal-title":"Cancer Res."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0070","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S1470-2045(09)70003-8","article-title":"Efficacy of azacitidine compared with that of conventional care regimens in the treatment of higher-risk myelodysplastic syndromes: a randomised, open-label, phase III study","volume":"10","author":"Fenaux","year":"2009","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0075","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/j.cgh.2013.09.053","article-title":"Factors that affect accuracy of alpha-fetoprotein test in detection of hepatocellular carcinoma in patients with cirrhosis","volume":"12","author":"Gopal","year":"2014","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0080","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0003759","article-title":"Sensitive detection of colorectal cancer in peripheral blood by septin 9 DNA methylation assay","volume":"3","author":"Grutzmann","year":"2008","journal-title":"PLoS One"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0085","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/path.1654","article-title":"The pathobiology of the septin gene family","volume":"204","author":"Hall","year":"2004","journal-title":"J. Pathol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0090","doi-asserted-by":"crossref","first-page":"7414","DOI":"10.1073\/pnas.1703577114","article-title":"DNA methylation markers for diagnosis and prognosis of common cancers","volume":"114","author":"Hao","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0095","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1136\/bmj.327.7414.557","article-title":"Measuring inconsistency in meta-analyses","volume":"327","author":"Higgins","year":"2003","journal-title":"BMJ"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0100","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s11010-015-2395-1","article-title":"Effects of 5\u2032-azacytidine and alendronate on a hepatocellular carcinoma cell line: a proteomics perspective","volume":"405","author":"Ilyas","year":"2015","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0105","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1016\/j.jhep.2011.12.001","volume":"56","year":"2012","journal-title":"J. Hepatol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0110","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/toxsci\/kfq307","article-title":"Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis","volume":"119","author":"Kakehashi","year":"2011","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0115","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1002\/cncr.21792","article-title":"Decitabine improves patient outcomes in myelodysplastic syndromes","volume":"106","author":"Kantarjian","year":"2006","journal-title":"Cancer"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0120","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.gie.2016.12.009","article-title":"Risk of colorectal cancer in chronic liver diseases: a systematic review and meta-analysis","volume":"86","author":"Komaki","year":"2017","journal-title":"Gastrointest. Endosc."},{"issue":"4","key":"10.1016\/j.ebiom.2018.03.029_bb0125","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s10654-011-9567-4","article-title":"PredictABEL: an R package for the assessment of risk prediction models","volume":"26","author":"Kundu","year":"2011","journal-title":"Eur. J. Epidemiol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0130","doi-asserted-by":"crossref","first-page":"122","DOI":"10.7326\/M13-1522","article-title":"Net reclassification improvement: computation, interpretation, and controversies: a literature review and clinician's guide","volume":"160","author":"Leening","year":"2014","journal-title":"Ann. Intern. Med."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0135","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1001\/jamainternmed.2014.1059","article-title":"Medicine's uncomfortable relationship with math: calculating positive predictive value","volume":"174","author":"Manrai","year":"2014","journal-title":"JAMA Intern. Med."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0140","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1002\/sim.2677","article-title":"Confidence intervals for predictive values with an emphasis to case-control studies","volume":"26","author":"Mercaldo","year":"2007","journal-title":"Stat. Med."},{"issue":"11","key":"10.1016\/j.ebiom.2018.03.029_bb0145","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1038\/nrclinonc.2017.97","article-title":"Precision medicine based on epigenomics: the paradigm of carcinoma of unknown primary","volume":"14","author":"Moran","year":"2017","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0150","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/nrm3284","article-title":"Septins: the fourth component of the cytoskeleton","volume":"13","author":"Mostowy","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0155","doi-asserted-by":"crossref","DOI":"10.1097\/MD.0000000000001774","article-title":"Diagnostic accuracy of procalcitonin for predicting blood culture results in patients with suspected bloodstream infection: an observational study of 35,343 consecutive patients (a STROBE-compliant article)","volume":"94","author":"Oussalah","year":"2015","journal-title":"Med. (Baltimore)"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0160","doi-asserted-by":"crossref","first-page":"62842","DOI":"10.18632\/oncotarget.11327","article-title":"BRIP1 coding variants are associated with a high risk of hepatocellularcarcinoma occurrence in patients with HCV- or HBV-related liver disease","volume":"8","author":"Oussalah","year":"2016","journal-title":"Oncotarget."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0165","first-page":"4729","article-title":"Isolation and mapping of a human septin gene to a region on chromosome 17q, commonly deleted in sporadic epithelial ovarian tumors","volume":"60","author":"Russell","year":"2000","journal-title":"Cancer Res."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0170","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1136\/bmj.324.7336.539","article-title":"The architecture of diagnostic research","volume":"324","author":"Sackett","year":"2002","journal-title":"BMJ"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0175","doi-asserted-by":"crossref","first-page":"4688","DOI":"10.1038\/sj.onc.1208574","article-title":"Multimodality expression profiling shows SEPT9 to be overexpressed in a wide range of human tumours","volume":"24","author":"Scott","year":"2005","journal-title":"Oncogene"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0180","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1002\/hep.510280404","article-title":"Risk of liver and other types of cancer in patients with cirrhosis: a nationwide cohort study in Denmark","volume":"28","author":"Sorensen","year":"1998","journal-title":"Hepatology"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0185","series-title":"R: A Language and Environment for Statistical Computing","author":"Team RC","year":"2013"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0190","doi-asserted-by":"crossref","DOI":"10.1126\/science.1260419","article-title":"Proteomics. Tissue-based map of the human proteome","volume":"347","author":"Uhlen","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0195","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1002\/hep.27732","article-title":"DNA methylation-based prognosis and epidrivers in hepatocellular carcinoma","volume":"61","author":"Villanueva","year":"2015","journal-title":"Hepatology"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0200","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1186\/1471-2407-13-398","article-title":"Aberrant septin 9 DNA methylation in colorectal cancer is restricted to a single CpG island","volume":"13","author":"Wasserkort","year":"2013","journal-title":"BMC Cancer"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0205","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1038\/nrm2407","article-title":"The septin family of GTPases: architecture and dynamics","volume":"9","author":"Weirich","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0210","first-page":"71","article-title":"Detection of aberrant p16 methylation in the plasma and serum of liver cancer patients","volume":"59","author":"Wong","year":"1999","journal-title":"Cancer Res."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0215","author":"World Health Organization"},{"key":"10.1016\/j.ebiom.2018.03.029_bb0220","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/nmat4997","article-title":"Circulating tumour DNA methylation markers for diagnosis and prognosis of hepatocellular carcinoma","volume":"16","author":"Xu","year":"2017","journal-title":"Nat. Mater."},{"key":"10.1016\/j.ebiom.2018.03.029_bb0225","series-title":"Statistical Methods in Diagnostic Medicine","author":"Zhou","year":"2009"}],"container-title":["eBioMedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396418301166?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396418301166?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T18:51:02Z","timestamp":1762455062000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352396418301166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":45,"alternative-id":["S2352396418301166"],"URL":"https:\/\/doi.org\/10.1016\/j.ebiom.2018.03.029","relation":{},"ISSN":["2352-3964"],"issn-type":[{"value":"2352-3964","type":"print"}],"subject":[],"published":{"date-parts":[[2018,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Plasma mSEPT9: A Novel Circulating Cell-free DNA-Based Epigenetic Biomarker to Diagnose Hepatocellular Carcinoma","name":"articletitle","label":"Article Title"},{"value":"eBioMedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ebiom.2018.03.029","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}