{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:08:46Z","timestamp":1791576526687,"version":"4.3.4"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T00:00:00Z","timestamp":1553817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006285","name":"Magnus Bergvalls Stiftelse","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010823","name":"Tore Nilsons Stiftelse f\u00f6r Medicinsk Forskning","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007232","name":"Radiumhemmets Forskningsfonder","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007232","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["EBioMedicine"],"published-print":{"date-parts":[[2019,5]]},"DOI":"10.1016\/j.ebiom.2019.03.089","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T17:32:12Z","timestamp":1554744732000},"page":"150-158","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":35,"special_numbering":"C","title":["Single-cell analysis reveals the KIT D816V mutation in haematopoietic stem and progenitor cells in systemic mastocytosis"],"prefix":"10.1016","volume":"43","author":[{"given":"Jennine","family":"Grootens","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna S.","family":"Ungerstedt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Ekoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elin","family":"R\u00f6nnberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Klimkowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rose-Marie","family":"Amini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Arock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stina","family":"S\u00f6derlund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattias","family":"Mattsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6795-5512","authenticated-orcid":false,"given":"Gunnar","family":"Nilsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3007-9875","authenticated-orcid":false,"given":"Joakim S.","family":"Dahlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.ebiom.2019.03.089_bb0005","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.molimm.2014.01.018","article-title":"Mast cell progenitors: origin, development and migration to tissues","volume":"63","author":"Dahlin","year":"2015","journal-title":"Mol Immunol"},{"issue":"17","key":"10.1016\/j.ebiom.2019.03.089_bb0010","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1182\/bloodadvances.2018019539","article-title":"Deciphering the differentiation trajectory from hematopoietic stem cells to mast cells","volume":"2","author":"Grootens","year":"2018","journal-title":"Blood Adv"},{"issue":"12","key":"10.1016\/j.ebiom.2019.03.089_bb0015","doi-asserted-by":"crossref","first-page":"3009","DOI":"10.1182\/blood.V80.12.3009.3009","article-title":"Recombinant human stem cell factor stimulates differentiation of mast cells from dispersed human fetal liver cells","volume":"80","author":"Irani","year":"1992","journal-title":"Blood"},{"issue":"3","key":"10.1016\/j.ebiom.2019.03.089_bb0020","doi-asserted-by":"crossref","first-page":"772","DOI":"10.4049\/jimmunol.148.3.772","article-title":"Effect of IL-3 and stem cell factor on the appearance of human basophils and mast cells from CD34+ pluripotent progenitor cells","volume":"148","author":"Kirshenbaum","year":"1992","journal-title":"J Immunol"},{"issue":"9","key":"10.1016\/j.ebiom.2019.03.089_bb0025","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1182\/blood.V80.9.2237.2237","article-title":"Induction of differentiation of human mast cells from bone marrow and peripheral blood mononuclear cells by recombinant human stem cell factor\/kit-ligand in long-term culture","volume":"80","author":"Valent","year":"1992","journal-title":"Blood"},{"issue":"2","key":"10.1016\/j.ebiom.2019.03.089_bb0030","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1073\/pnas.90.2.735","article-title":"Development of human mast cells from umbilical cord blood cells by recombinant human and murine c-kit ligand","volume":"90","author":"Mitsui","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"23","key":"10.1016\/j.ebiom.2019.03.089_bb0035","doi-asserted-by":"crossref","first-page":"10560","DOI":"10.1073\/pnas.92.23.10560","article-title":"Identification of a point mutation in the catalytic domain of the Protooncogene C-kit in peripheral-blood mononuclear-cells of patients who have mastocytosis with an associated hematologic disorder","volume":"92","author":"Nagata","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"10.1016\/j.ebiom.2019.03.089_bb0040","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1111\/j.1365-2362.2007.01807.x","article-title":"Standards and standardization in mastocytosis: consensus statements on diagnostics, treatment recommendations and response criteria","volume":"37","author":"Valent","year":"2007","journal-title":"Eur J Clin Invest"},{"issue":"11","key":"10.1016\/j.ebiom.2019.03.089_bb0045","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1182\/blood-2016-09-731893","article-title":"Mastocytosis: 2016 updated WHO classification and novel emerging treatment concepts","volume":"129","author":"Valent","year":"2017","journal-title":"Blood"},{"issue":"6","key":"10.1016\/j.ebiom.2019.03.089_bb0050","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1038\/leu.2015.24","article-title":"KIT mutation analysis in mast cell neoplasms: recommendations of the European competence network on mastocytosis","volume":"29","author":"Arock","year":"2015","journal-title":"Leukemia"},{"issue":"2","key":"10.1016\/j.ebiom.2019.03.089_bb0055","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.jmoldx.2010.10.004","article-title":"Improved detection of the KIT D816V mutation in patients with systemic mastocytosis using a quantitative and highly sensitive real-time qPCR assay","volume":"13","author":"Kristensen","year":"2011","journal-title":"J Mol Diagn"},{"issue":"4","key":"10.1016\/j.ebiom.2019.03.089_bb0060","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s002770050439","article-title":"Multilineage involvement and erythropoietin-\"independent\" erythroid progenitor cells in a patient with systemic mastocytosis","volume":"77","author":"Afonja","year":"1998","journal-title":"Ann Hematol"},{"issue":"7","key":"10.1016\/j.ebiom.2019.03.089_bb0065","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1182\/blood-2006-04-015545","article-title":"KIT mutation in mast cells and other bone marrow hematopoietic cell lineages in systemic mast cell disorders: a prospective study of the Spanish network on Mastocytosis (REMA) in a series of 113 patients","volume":"108","author":"Garcia-Montero","year":"2006","journal-title":"Blood"},{"issue":"6","key":"10.1016\/j.ebiom.2019.03.089_bb0070","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/j.jaci.2005.02.030","article-title":"Analysis of the lineage relationship between mast cells and basophils using the c-kit D816V mutation as a biologic signature","volume":"115","author":"Kocabas","year":"2005","journal-title":"J Allergy Clin Immunol"},{"issue":"2","key":"10.1016\/j.ebiom.2019.03.089_bb0075","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1182\/blood-2002-01-0203","article-title":"Evidence for the involvement of a hematopoietic progenitor cell in systemic mastocytosis from single-cell analysis of mutations in the c-kit gene","volume":"100","author":"Yavuz","year":"2002","journal-title":"Blood"},{"issue":"10","key":"10.1016\/j.ebiom.2019.03.089_bb0080","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/S0145-2126(03)00065-1","article-title":"Eosinophils are derived from the neoplastic clone in patients with systemic mastocytosis and eosinophilia","volume":"27","author":"Pardanani","year":"2003","journal-title":"Leuk Res"},{"issue":"6","key":"10.1016\/j.ebiom.2019.03.089_bb0085","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1111\/all.13413","article-title":"Characterization of CD34(+) hematopoietic cells in systemic mastocytosis: potential role in disease dissemination","volume":"73","author":"Mayado","year":"2018","journal-title":"Allergy"},{"issue":"5","key":"10.1016\/j.ebiom.2019.03.089_bb0090","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1038\/leu.2015.4","article-title":"Molecular profiling of myeloid progenitor cells in multi-mutated advanced systemic mastocytosis identifies KIT D816V as a distinct and late event","volume":"29","author":"Jawhar","year":"2015","journal-title":"Leukemia"},{"issue":"8","key":"10.1016\/j.ebiom.2019.03.089_bb0095","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1038\/modpathol.2015.72","article-title":"Detection of the KIT D816V mutation in peripheral blood of systemic mastocytosis: diagnostic implications","volume":"28","author":"Jara-Acevedo","year":"2015","journal-title":"Mod Pathol"},{"issue":"20","key":"10.1016\/j.ebiom.2019.03.089_bb0100","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1182\/blood-2016-03-643544","article-title":"The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia","volume":"127","author":"Arber","year":"2016","journal-title":"Blood"},{"issue":"1","key":"10.1016\/j.ebiom.2019.03.089_bb0105","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1182\/blood-2013-10-534685","article-title":"A new human mast cell line expressing a functional IgE receptor converts to tumorigenic growth by KIT D816V transfection","volume":"124","author":"Saleh","year":"2014","journal-title":"Blood"},{"issue":"1","key":"10.1016\/j.ebiom.2019.03.089_bb0110","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1046\/j.1365-2567.2003.01559.x","article-title":"Functional and phenotypic studies of two variants of a human mast cell line with a distinct set of mutations in the c-kit proto-oncogene","volume":"108","author":"Sundstrom","year":"2003","journal-title":"Immunology"},{"issue":"16","key":"10.1016\/j.ebiom.2019.03.089_bb0115","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1182\/blood-2017-03-773374","article-title":"KIT signaling is dispensable for human mast cell progenitor development","volume":"130","author":"Dahlin","year":"2017","journal-title":"Blood"},{"issue":"6","key":"10.1016\/j.ebiom.2019.03.089_bb0120","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/BF00426615","article-title":"Immunoperoxidase and enzyme-histochemical demonstration of human skin tryptase in cutaneous mast cells in normal and mastocytoma skin","volume":"280","author":"Harvima","year":"1988","journal-title":"Arch Dermatol Res"},{"issue":"9","key":"10.1016\/j.ebiom.2019.03.089_bb0125","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1038\/leu.2016.105","article-title":"Detection of circulating mast cells in advanced systemic mastocytosis","volume":"30","author":"Dahlin","year":"2016","journal-title":"Leukemia"},{"issue":"6039","key":"10.1016\/j.ebiom.2019.03.089_bb0130","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1126\/science.1201219","article-title":"Isolation of single human hematopoietic stem cells capable of long-term multilineage engraftment","volume":"333","author":"Notta","year":"2011","journal-title":"Science"},{"issue":"6","key":"10.1016\/j.ebiom.2019.03.089_bb0135","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.stem.2007.10.001","article-title":"Identification of a hierarchy of multipotent hietematopoic progenitors in human cord blood","volume":"1","author":"Majeti","year":"2007","journal-title":"Cell Stem Cell"},{"issue":"18","key":"10.1016\/j.ebiom.2019.03.089_bb0140","doi-asserted-by":"crossref","first-page":"11872","DOI":"10.1073\/pnas.172384399","article-title":"Prospective isolation of human clonogenic common myeloid progenitors","volume":"99","author":"Manz","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"10.1016\/j.ebiom.2019.03.089_bb0145","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.ccr.2010.12.012","article-title":"Coexistence of LMPP-like and GMP-like leukemia stem cells in acute myeloid leukemia","volume":"19","author":"Goardon","year":"2011","journal-title":"Cancer Cell"},{"issue":"3","key":"10.1016\/j.ebiom.2019.03.089_bb0150","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1084\/jem.20141442","article-title":"Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow","volume":"212","author":"Lee","year":"2015","journal-title":"J Exp Med"},{"issue":"23","key":"10.1016\/j.ebiom.2019.03.089_bb0155","doi-asserted-by":"crossref","first-page":"12551","DOI":"10.1073\/pnas.94.23.12551","article-title":"Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin","volume":"94","author":"Olweus","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"10.1016\/j.ebiom.2019.03.089_bb0160","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/ni.1889","article-title":"Revised map of the human progenitor hierarchy shows the origin of macrophages and dendritic cells in early lymphoid development","volume":"11","author":"Doulatov","year":"2010","journal-title":"Nat Immunol"},{"issue":"4","key":"10.1016\/j.ebiom.2019.03.089_bb0165","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1182\/blood-2015-06-650648","article-title":"Lin- CD34hi CD117int\/hi FcepsilonRI+ cells in human blood constitute a rare population of mast cell progenitors","volume":"127","author":"Dahlin","year":"2016","journal-title":"Blood"},{"issue":"5","key":"10.1016\/j.ebiom.2019.03.089_bb0170","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1016\/j.celrep.2013.04.025","article-title":"Revision of the human hematopoietic tree: granulocyte subtypes derive from distinct hematopoietic lineages","volume":"3","author":"G\u00f6rgens","year":"2013","journal-title":"Cell Rep"},{"issue":"1","key":"10.1016\/j.ebiom.2019.03.089_bb0175","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1189\/jlb.5RU0614-296R","article-title":"The immunophenotype of mast cells and its utility in the diagnostic work-up of systemic mastocytosis","volume":"97","author":"Teodosio","year":"2015","journal-title":"J Leukoc Biol"},{"issue":"4","key":"10.1016\/j.ebiom.2019.03.089_bb0180","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/modpathol.2010.224","article-title":"Aberrant expression of CD30 in neoplastic mast cells in high-grade mastocytosis","volume":"24","author":"Sotlar","year":"2011","journal-title":"Mod Pathol"},{"issue":"8","key":"10.1016\/j.ebiom.2019.03.089_bb0185","doi-asserted-by":"crossref","first-page":"3540","DOI":"10.1096\/fj.14-250894","article-title":"CD52 is a molecular target in advanced systemic mastocytosis","volume":"28","author":"Hoermann","year":"2014","journal-title":"FASEB J"},{"issue":"4","key":"10.1016\/j.ebiom.2019.03.089_bb0190","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1309\/AJCPZ3J4GEEYIRRA","article-title":"Mast cells in systemic mastocytosis have distinctly brighter CD45 expression by flow cytometry","volume":"143","author":"Chisholm","year":"2015","journal-title":"Am J Clin Pathol"},{"issue":"1","key":"10.1016\/j.ebiom.2019.03.089_bb0195","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/cyto.b.10072","article-title":"Immunophenotypic analysis of mast cells in mastocytosis: when and how to do it. Proposals of the Spanish network on Mastocytosis (REMA)","volume":"58","author":"Escribano","year":"2004","journal-title":"Cytometry B Clin Cytom"},{"issue":"4","key":"10.1016\/j.ebiom.2019.03.089_bb0200","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1038\/modpathol.2011.192","article-title":"Immunophenotyping in systemic mastocytosis diagnosis: 'CD25 positive' alone is more informative than the 'CD25 and\/or CD2' WHO criterion","volume":"25","author":"Morgado","year":"2012","journal-title":"Mod Pathol"},{"issue":"3","key":"10.1016\/j.ebiom.2019.03.089_bb0205","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/j.jaci.2009.10.020","article-title":"Mast cells from different molecular and prognostic subtypes of systemic mastocytosis display distinct immunophenotypes","volume":"125","author":"Teodosio","year":"2010","journal-title":"J Allergy Clin Immunol"}],"container-title":["EBioMedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396419302348?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396419302348?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T18:53:58Z","timestamp":1762455238000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352396419302348"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5]]},"references-count":41,"alternative-id":["S2352396419302348"],"URL":"https:\/\/doi.org\/10.1016\/j.ebiom.2019.03.089","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/394304","asserted-by":"object"}]},"ISSN":["2352-3964"],"issn-type":[{"value":"2352-3964","type":"print"}],"subject":[],"published":{"date-parts":[[2019,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-cell analysis reveals the KIT D816V mutation in haematopoietic stem and progenitor cells in systemic mastocytosis","name":"articletitle","label":"Article Title"},{"value":"EBioMedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ebiom.2019.03.089","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}