{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T21:59:00Z","timestamp":1780610340355,"version":"3.54.1"},"reference-count":88,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T00:00:00Z","timestamp":1590451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003500","name":"Universit\u00e0 degli Studi di Padova","doi-asserted-by":"publisher","award":["BIRD187130"],"award-info":[{"award-number":["BIRD187130"]}],"id":[{"id":"10.13039\/501100003500","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002426","name":"Fondazione Telethon","doi-asserted-by":"publisher","award":["GGP19148"],"award-info":[{"award-number":["GGP19148"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["16DZ1910200"],"award-info":[{"award-number":["16DZ1910200"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004462","name":"Consiglio Nazionale delle Ricerche","doi-asserted-by":"publisher","award":["DSB.AD008.370.003 \\\\TERABIO-IBCN"],"award-info":[{"award-number":["DSB.AD008.370.003 \\\\TERABIO-IBCN"]}],"id":[{"id":"10.13039\/501100004462","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770776"],"award-info":[{"award-number":["31770776"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["EBioMedicine"],"published-print":{"date-parts":[[2020,7]]},"DOI":"10.1016\/j.ebiom.2020.102825","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T04:03:56Z","timestamp":1592193836000},"page":"102825","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":34,"special_numbering":"C","title":["A potent antagonist antibody targeting connexin hemichannels alleviates Clouston syndrome symptoms in mutant mice"],"prefix":"10.1016","volume":"57","author":[{"given":"Yuanyuan","family":"Kuang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Zorzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damiano","family":"Buratto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaia","family":"Ziraldo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Mazzarda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Peres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4084-4932","authenticated-orcid":false,"given":"Chiara","family":"Nardin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Salvatore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Chiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferdinando","family":"Scavizzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello","family":"Raspa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Qiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youjun","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojie","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaru","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Zonta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Lerner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3751-1691","authenticated-orcid":false,"given":"Fabio","family":"Mammano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.ebiom.2020.102825_bib0001","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.bbamem.2017.04.024","article-title":"Human diseases associated with connexin mutations","volume":"1860","author":"Srinivas","year":"2018","journal-title":"Biochimica et biophysica acta"},{"key":"10.1016\/j.ebiom.2020.102825_bib0002","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.semcdb.2015.11.018","article-title":"Connexin channels in congenital skin disorders","volume":"50","author":"Lilly","year":"2016","journal-title":"Seminars in cell & developmental biology"},{"issue":"7","key":"10.1016\/j.ebiom.2020.102825_bib0003","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a033233","article-title":"Inner Ear Connexin Channels: Roles in Development and Maintenance of Cochlear Function","volume":"9","author":"Mammano","year":"2019","journal-title":"Cold Spring Harbor perspectives in medicine"},{"key":"10.1016\/j.ebiom.2020.102825_bib0004","doi-asserted-by":"crossref","first-page":"267","DOI":"10.3389\/fncel.2015.00267","article-title":"Diseases associated with leaky hemichannels","volume":"9","author":"Retamal","year":"2015","journal-title":"Frontiers in cellular neuroscience"},{"issue":"3","key":"10.1016\/j.ebiom.2020.102825_bib0005","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.jid.2015.11.017","article-title":"From Hyperactive Connexin26 Hemichannels to Impairments in Epidermal Calcium Gradient and Permeability Barrier in the Keratitis-Ichthyosis-Deafness Syndrome","volume":"136","author":"Garcia","year":"2016","journal-title":"J Invest Dermatol"},{"issue":"30","key":"10.1016\/j.ebiom.2020.102825_bib0006","doi-asserted-by":"crossref","first-page":"17903","DOI":"10.1074\/jbc.271.30.17903","article-title":"Molecular cloning and functional expression of mouse connexin-30,a gap junction gene highly expressed in adult brain and skin","volume":"271","author":"Dahl","year":"1996","journal-title":"The Journal of biological chemistry"},{"issue":"11-12","key":"10.1016\/j.ebiom.2020.102825_bib0007","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1078\/0171-9335-00422","article-title":"Expression and function of connexins in the epidermis, analyzed with transgenic mouse mutants","volume":"83","author":"Kretz","year":"2004","journal-title":"European journal of cell biology"},{"issue":"Pt 16","key":"10.1016\/j.ebiom.2020.102825_bib0008","doi-asserted-by":"crossref","first-page":"3443","DOI":"10.1242\/jcs.00638","article-title":"Altered connexin expression and wound healing in the epidermis of connexin-deficient mice","volume":"116","author":"Kretz","year":"2003","journal-title":"Journal of cell science"},{"issue":"4","key":"10.1016\/j.ebiom.2020.102825_bib0009","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1111\/j.1365-2133.2006.07439.x","article-title":"Connexin 30, a new marker of hyperproliferative epidermis","volume":"155","author":"Lemaitre","year":"2006","journal-title":"The British journal of dermatology"},{"issue":"16","key":"10.1016\/j.ebiom.2020.102825_bib0010","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1093\/hmg\/ddh191","article-title":"Connexin30 mutations responsible for hidrotic ectodermal dysplasia cause abnormal hemichannel activity","volume":"13","author":"Essenfelder","year":"2004","journal-title":"Human molecular genetics"},{"issue":"2","key":"10.1016\/j.ebiom.2020.102825_bib0011","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/79851","article-title":"Mutations in GJB6 cause hidrotic ectodermal dysplasia","volume":"26","author":"Lamartine","year":"2000","journal-title":"Nature genetics"},{"issue":"6","key":"10.1016\/j.ebiom.2020.102825_bib0012","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1111\/j.1346-8138.2009.00768.x","article-title":"G11R mutation in GJB6 gene causes hidrotic ectodermal dysplasia involving only hair and nails in a Chinese family","volume":"37","author":"Chen","year":"2010","journal-title":"The Journal of dermatology"},{"issue":"11","key":"10.1016\/j.ebiom.2020.102825_bib0013","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1001\/jamadermatol.2013.4766","article-title":"Clouston syndrome with heterozygous GJB6 mutation p.Ala88Val and GJB2 variant p.Val27Ile revealing mild sensorineural hearing loss and photophobia","volume":"149","author":"Sugiura","year":"2013","journal-title":"JAMA dermatology"},{"issue":"8","key":"10.1016\/j.ebiom.2020.102825_bib0014","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1111\/jdv.13600","article-title":"A known mutation in GJB6 in a large Chinese family with hidrotic ectodermal dysplasia","volume":"30","author":"Yang","year":"2016","journal-title":"Journal of the European Academy of Dermatology and Venereology: JEADV"},{"issue":"Pt 8","key":"10.1016\/j.ebiom.2020.102825_bib0015","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1242\/jcs.138230","article-title":"Mutations in Cx30 that are linked to skin disease and non-syndromic hearing loss exhibit several distinct cellular pathologies","volume":"127","author":"Berger","year":"2014","journal-title":"Journal of cell science"},{"issue":"3","key":"10.1016\/j.ebiom.2020.102825_bib0016","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1083\/jcb.106.3.761","article-title":"Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line","volume":"106","author":"Boukamp","year":"1988","journal-title":"The Journal of cell biology"},{"issue":"9","key":"10.1016\/j.ebiom.2020.102825_bib0017","doi-asserted-by":"crossref","first-page":"e7560","DOI":"10.1590\/1414-431x20187560","article-title":"Mechanistic effect of the human GJB6 gene and its mutations in HaCaT cell proliferation and apoptosis","volume":"51","author":"Lu","year":"2018","journal-title":"Braz J Med Biol Res"},{"key":"10.1016\/j.ebiom.2020.102825_bib0018","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1749-6632.2009.04363.x","article-title":"Making an epidermis","volume":"1170","author":"Koster","year":"2009","journal-title":"Annals of the New York Academy of Sciences"},{"key":"10.1016\/j.ebiom.2020.102825_bib0019","series-title":"Current Topics in Developmental Biology","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/bs.ctdb.2015.11.033","article-title":"Chapter Nineteen - Epithelial Skin Biology: Three Decades of Developmental Biology, a Hundred Questions Answered and a Thousand New Ones to Address","author":"Fuchs","year":"2016"},{"issue":"1","key":"10.1016\/j.ebiom.2020.102825_bib0020","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0092-8674(80)90406-7","article-title":"Calcium regulation of growth and differentiation of mouse epidermal cells in culture","volume":"19","author":"Hennings","year":"1980","journal-title":"Cell"},{"issue":"6","key":"10.1016\/j.ebiom.2020.102825_bib0021","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1684\/ejd.2014.2452","article-title":"Calcium\u2013a central regulator of keratinocyte differentiation in health and disease","volume":"24","author":"Elsholz","year":"2014","journal-title":"European journal of dermatology: EJD"},{"issue":"17","key":"10.1016\/j.ebiom.2020.102825_bib0022","doi-asserted-by":"crossref","first-page":"2124","DOI":"10.1080\/15384101.2019.1642066","article-title":"Multi-omics profiling of calcium-induced human keratinocytes differentiation reveals modulation of unfolded protein response signaling pathways","volume":"18","author":"Michaletti","year":"2019","journal-title":"Cell cycle"},{"issue":"3","key":"10.1016\/j.ebiom.2020.102825_bib0023","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1007\/s00441-014-2094-3","article-title":"Connexins and skin disease: insights into the role of beta connexins in skin homeostasis","volume":"360","author":"Martin","year":"2015","journal-title":"Cell and tissue research"},{"issue":"6","key":"10.1016\/j.ebiom.2020.102825_bib0024","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2017.08.034","article-title":"SnapShot: Connexins and Disease","volume":"170","author":"Laird","year":"2017","journal-title":"Cell"},{"issue":"33","key":"10.1016\/j.ebiom.2020.102825_bib0025","doi-asserted-by":"crossref","first-page":"29667","DOI":"10.1074\/jbc.M505381200","article-title":"Human keratinocytes release ATP and utilize three mechanisms for nucleotide interconversion at the cell surface","volume":"280","author":"Burrell","year":"2005","journal-title":"The Journal of biological chemistry"},{"issue":"2","key":"10.1016\/j.ebiom.2020.102825_bib0026","doi-asserted-by":"crossref","first-page":"e56744","DOI":"10.1371\/journal.pone.0056744","article-title":"Air-stimulated ATP release from keratinocytes occurs through connexin hemichannels","volume":"8","author":"Barr","year":"2013","journal-title":"PloS one"},{"issue":"10","key":"10.1016\/j.ebiom.2020.102825_bib0027","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.1038\/jid.2013.163","article-title":"In vivo imaging demonstrates ATP release from murine keratinocytes and its involvement in cutaneous inflammation after tape stripping","volume":"133","author":"Takahashi","year":"2013","journal-title":"J Invest Dermatol"},{"issue":"Pt 19","key":"10.1016\/j.ebiom.2020.102825_bib0028","first-page":"4159","article-title":"Mechanosensitive ATP release from hemichannels and Ca(2)(+) influx through TRPC6 accelerate wound closure in keratinocytes","volume":"127","author":"Takada","year":"2014","journal-title":"Journal of cell science"},{"issue":"2","key":"10.1016\/j.ebiom.2020.102825_bib0029","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0923-1811(00)00117-1","article-title":"Purinoceptor-mediated calcium mobilization and proliferation in HaCaT keratinocytes","volume":"25","author":"Lee","year":"2001","journal-title":"Journal of dermatological science"},{"issue":"3 Pt 1","key":"10.1016\/j.ebiom.2020.102825_bib0030","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1038\/jid.2011.344","article-title":"Purinergic signaling in healthy and diseased skin","volume":"132","author":"Burnstock","year":"2012","journal-title":"J Invest Dermatol"},{"issue":"9","key":"10.1016\/j.ebiom.2020.102825_bib0031","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1016\/j.febslet.2014.03.040","article-title":"The Clouston syndrome mutation connexin30 A88V leads to hyperproliferation of sebaceous glands and hearing impairments in mice","volume":"588","author":"Bosen","year":"2014","journal-title":"FEBS letters"},{"issue":"3","key":"10.1016\/j.ebiom.2020.102825_bib0032","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.cell.2006.06.048","article-title":"Blimp1 defines a progenitor population that governs cellular input to the sebaceous gland","volume":"126","author":"Horsley","year":"2006","journal-title":"Cell"},{"issue":"2","key":"10.1016\/j.ebiom.2020.102825_bib0033","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.224097","article-title":"The connexin 30 A88V mutant reduces cochlear gap junction expression and confers long-term protection against hearing loss","volume":"132","author":"Kelly","year":"2019","journal-title":"Journal of cell science"},{"key":"10.1016\/j.ebiom.2020.102825_bib0034","doi-asserted-by":"crossref","first-page":"14530","DOI":"10.1038\/ncomms14530","article-title":"A connexin30 mutation rescues hearing and reveals roles for gap junctions in cochlear amplification and micromechanics","volume":"8","author":"Lukashkina","year":"2017","journal-title":"Nature communications"},{"key":"10.1016\/j.ebiom.2020.102825_bib0035","doi-asserted-by":"crossref","first-page":"298","DOI":"10.3389\/fnmol.2017.00298","article-title":"Design and Characterization of a Human Monoclonal Antibody that Modulates Mutant Connexin 26 Hemichannels Implicated in Deafness and Skin Disorders","volume":"10","author":"Xu","year":"2017","journal-title":"Frontiers in molecular neuroscience"},{"key":"10.1016\/j.ebiom.2020.102825_bib0036","doi-asserted-by":"crossref","first-page":"392","DOI":"10.3389\/fphys.2019.00392","article-title":"A Human-Derived Monoclonal Antibody Targeting Extracellular Connexin Domain Selectively Modulates Hemichannel Function","volume":"10","author":"Ziraldo","year":"2019","journal-title":"Frontiers in physiology"},{"key":"10.1016\/j.ebiom.2020.102825_bib0037","article-title":"Monoclonal Antibodies: A Review","author":"Singh","year":"2017","journal-title":"Current clinical pharmacology"},{"issue":"3","key":"10.1016\/j.ebiom.2020.102825_bib0038","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1038\/nrd.2017.227","article-title":"Next generation antibody drugs: pursuit of the 'high-hanging fruit'","volume":"17","author":"Carter","year":"2018","journal-title":"Nat Rev Drug Discov"},{"issue":"12","key":"10.1016\/j.ebiom.2020.102825_bib0039","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0969-2126(94)00123-5","article-title":"Crystal structure of a diabody, a bivalent antibody fragment","volume":"2","author":"Perisic","year":"1994","journal-title":"Structure"},{"key":"10.1016\/j.ebiom.2020.102825_bib0040","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/978-1-62703-992-5_20","article-title":"Reformatting of scFv antibodies into the scFv-Fc format and their downstream purification","volume":"1131","author":"Bujak","year":"2014","journal-title":"Methods in molecular biology"},{"issue":"1","key":"10.1016\/j.ebiom.2020.102825_bib0041","first-page":"152","article-title":"Differential expression of the gap junction proteins connexin45, -43, -40, -31, and -26 in mouse skin","volume":"65","author":"Butterweck","year":"1994","journal-title":"European journal of cell biology"},{"issue":"2","key":"10.1016\/j.ebiom.2020.102825_bib0042","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1111\/1523-1747.ep12393218","article-title":"Topography of mammalian connexins in human skin","volume":"103","author":"Salomon","year":"1994","journal-title":"J Invest Dermatol"},{"issue":"1","key":"10.1016\/j.ebiom.2020.102825_bib0043","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/aja.1002000102","article-title":"Expression of gap junction proteins Cx26, Cx31.1, Cx37, and Cx43 in developing and mature rat epidermis","volume":"200","author":"Goliger","year":"1994","journal-title":"Developmental dynamics: an official publication of the American Association of Anatomists"},{"issue":"4","key":"10.1016\/j.ebiom.2020.102825_bib0044","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1046\/j.0022-202x.2001.01468.x","article-title":"Multiple epidermal connexins are expressed in different keratinocyte subpopulations including connexin 31","volume":"117","author":"Di","year":"2001","journal-title":"J Invest Dermatol"},{"issue":"15","key":"10.1016\/j.ebiom.2020.102825_bib0045","doi-asserted-by":"crossref","first-page":"2937","DOI":"10.1007\/s00018-015-1969-0","article-title":"Connexins and pannexins in the integumentary system: the skin and appendages","volume":"72","author":"Faniku","year":"2015","journal-title":"Cellular and molecular life sciences: CMLS"},{"key":"10.1016\/j.ebiom.2020.102825_bib0046","doi-asserted-by":"crossref","first-page":"520","DOI":"10.3389\/fimmu.2014.00520","article-title":"IgG subclasses and allotypes: from structure to effector functions","volume":"5","author":"Vidarsson","year":"2014","journal-title":"Frontiers in immunology"},{"issue":"6","key":"10.1016\/j.ebiom.2020.102825_bib0047","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1000412","article-title":"Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research","volume":"8","author":"Kilkenny","year":"2010","journal-title":"PLoS biology"},{"issue":"2","key":"10.1016\/j.ebiom.2020.102825_bib0048","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1177\/0023677217724823","article-title":"PREPARE: guidelines for planning animal research and testing","volume":"52","author":"Smith","year":"2018","journal-title":"Laboratory animals"},{"issue":"4","key":"10.1016\/j.ebiom.2020.102825_bib0049","doi-asserted-by":"crossref","DOI":"10.1038\/jid.2012.457","article-title":"Topically applied flightless I neutralizing antibodies improve healing of blistered skin in a murine model of epidermolysis bullosa acquisita","volume":"133","author":"Kopecki","year":"2013","journal-title":"J Invest Dermatol"},{"issue":"7","key":"10.1016\/j.ebiom.2020.102825_bib0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.xphs.2017.03.012","article-title":"Development of Topical Delivery Systems for Flightless Neutralizing Antibody","volume":"106","author":"Haidari","year":"2017","journal-title":"Journal of pharmaceutical sciences"},{"issue":"1","key":"10.1016\/j.ebiom.2020.102825_bib0051","article-title":"Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes","volume":"109","author":"Engvall","year":"1972","journal-title":"J Immunol"},{"issue":"8","key":"10.1016\/j.ebiom.2020.102825_bib0052","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1016\/j.febslet.2014.02.004","article-title":"Differentiating connexin hemichannels and pannexin channels in cellular ATP release","volume":"588","author":"Lohman","year":"2014","journal-title":"FEBS letters"},{"issue":"125","key":"10.1016\/j.ebiom.2020.102825_bib0053","doi-asserted-by":"crossref","DOI":"10.3791\/56027","article-title":"Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin","author":"Li","year":"2017","journal-title":"Journal of visualized experiments: JoVE"},{"issue":"5","key":"10.1016\/j.ebiom.2020.102825_bib0054","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1111\/j.1476-5381.1996.tb15273.x","article-title":"Enhancement of sympathetic purinergic neurotransmission in the guinea-pig isolated vas deferens by the novel ecto-ATPase inhibitor ARL 67156","volume":"117","author":"Westfall","year":"1996","journal-title":"British journal of pharmacology"},{"key":"10.1016\/j.ebiom.2020.102825_bib0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.neuropharm.2013.03.040","article-title":"Regulation of connexin hemichannel activity by membrane potential and the extracellular calcium in health and disease","author":"Fasciani","year":"2013","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.ebiom.2020.102825_bib0056","series-title":"Introduction to Statistics","first-page":"473","author":"Lane","year":"2003"},{"key":"10.1016\/j.ebiom.2020.102825_bib0057","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ddtec.2016.09.004","article-title":"Translational pharmacokinetics and pharmacodynamics of monoclonal antibodies","volume":"21-22","author":"Kamath","year":"2016","journal-title":"Drug discovery today Technologies"},{"issue":"1","key":"10.1016\/j.ebiom.2020.102825_bib0058","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1080\/19420862.2015.1113360","article-title":"Pharmacokinetic properties of IgG and various Fc fusion proteins in mice","volume":"8","author":"Unverdorben","year":"2016","journal-title":"mAbs"},{"issue":"3","key":"10.1016\/j.ebiom.2020.102825_bib0059","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1083\/jcb.100.3.965","article-title":"Nile red: a selective fluorescent stain for intracellular lipid droplets","volume":"100","author":"Greenspan","year":"1985","journal-title":"The Journal of cell biology"},{"issue":"2","key":"10.1016\/j.ebiom.2020.102825_bib0060","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1111\/j.1468-3083.1998.tb00710.x","article-title":"Advances in the understanding of malignant transformation of keratinocytes: an immunohistochemical study","volume":"10","author":"Tucci","year":"1998","journal-title":"Journal of the European Academy of Dermatology and Venereology: JEADV"},{"issue":"3","key":"10.1016\/j.ebiom.2020.102825_bib0061","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1007\/s004240000294","article-title":"Electrical properties of gap junction hemichannels identified in transfected HeLa cells","volume":"440","author":"Valiunas","year":"2000","journal-title":"Pflugers Archiv: European journal of physiology"},{"issue":"1","key":"10.1016\/j.ebiom.2020.102825_bib0062","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/j.1460-2075.1989.tb03350.x","article-title":"Calmodulin is required for cell-cycle progression during G1 and mitosis","volume":"8","author":"Rasmussen","year":"1989","journal-title":"The EMBO journal"},{"issue":"15","key":"10.1016\/j.ebiom.2020.102825_bib0063","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1016\/S0960-9822(02)01019-9","article-title":"Centrin-2 is required for centriole duplication in mammalian cells","volume":"12","author":"Salisbury","year":"2002","journal-title":"Current biology: CB"},{"key":"10.1016\/j.ebiom.2020.102825_bib0064","doi-asserted-by":"crossref","first-page":"38281","DOI":"10.1038\/srep38281","article-title":"Calcium ions function as a booster of chromosome condensation","volume":"6","author":"Phengchat","year":"2016","journal-title":"Scientific reports"},{"issue":"12","key":"10.1016\/j.ebiom.2020.102825_bib0065","doi-asserted-by":"crossref","first-page":"14602","DOI":"10.1096\/fj.201901662R","article-title":"A centrosome-localized calcium signal is essential for mammalian cell mitosis","volume":"33","author":"Helassa","year":"2019","journal-title":"FASEB journal: official publication of the Federation of American Societies for Experimental Biology"},{"issue":"4","key":"10.1016\/j.ebiom.2020.102825_bib0066","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.tcb.2018.01.002","article-title":"Calcium Dynamics as a Machine for Decoding Signals","volume":"28","author":"Giorgi","year":"2018","journal-title":"Trends in cell biology"},{"issue":"5","key":"10.1016\/j.ebiom.2020.102825_bib0067","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1165\/rcmb.2008-0367OC","article-title":"Pannexin 1 contributes to ATP release in airway epithelia","volume":"41","author":"Ransford","year":"2009","journal-title":"American journal of respiratory cell and molecular biology"},{"issue":"4","key":"10.1016\/j.ebiom.2020.102825_bib0068","doi-asserted-by":"crossref","first-page":"H1146","DOI":"10.1152\/ajpheart.00301.2010","article-title":"Pannexin 1 is the conduit for low oxygen tension-induced ATP release from human erythrocytes","volume":"299","author":"Sridharan","year":"2010","journal-title":"American journal of physiology Heart and circulatory physiology"},{"issue":"10","key":"10.1016\/j.ebiom.2020.102825_bib0069","doi-asserted-by":"crossref","first-page":"H1208","DOI":"10.1152\/ajpheart.00251.2012","article-title":"Cardiomyocyte ATP release through pannexin 1 aids in early fibroblast activation","volume":"303","author":"Dolmatova","year":"2012","journal-title":"American journal of physiology Heart and circulatory physiology"},{"issue":"14","key":"10.1016\/j.ebiom.2020.102825_bib0070","doi-asserted-by":"crossref","first-page":"11303","DOI":"10.1074\/jbc.M111.323378","article-title":"Pannexin 1, an ATP release channel, is activated by caspase cleavage of its pore-associated C-terminal autoinhibitory region","volume":"287","author":"Sandilos","year":"2012","journal-title":"The Journal of biological chemistry"},{"issue":"3","key":"10.1016\/j.ebiom.2020.102825_bib0071","doi-asserted-by":"crossref","first-page":"C279","DOI":"10.1152\/ajpcell.00303.2017","article-title":"Cationic control of Panx1 channel function","volume":"315","author":"Wang","year":"2018","journal-title":"American journal of physiology Cell physiology"},{"issue":"24","key":"10.1016\/j.ebiom.2020.102825_bib0072","doi-asserted-by":"crossref","first-page":"4776","DOI":"10.1091\/mbc.e11-09-0778","article-title":"The Cx26-G45E mutation displays increased hemichannel activity in a mouse model of the lethal form of keratitis-ichthyosis-deafness syndrome","volume":"22","author":"Mese","year":"2011","journal-title":"Molecular biology of the cell"},{"issue":"15","key":"10.1016\/j.ebiom.2020.102825_bib0073","doi-asserted-by":"crossref","first-page":"1904","DOI":"10.1016\/j.febslet.2015.05.047","article-title":"Altered epidermal lipid processing and calcium distribution in the KID syndrome mouse model Cx26S17F","volume":"589","author":"Bosen","year":"2015","journal-title":"FEBS letters"},{"key":"10.1016\/j.ebiom.2020.102825_bib0074","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3389\/fnmol.2018.00227","article-title":"What's the Function of Connexin 32 in the Peripheral Nervous System","volume":"11","author":"Bortolozzi","year":"2018","journal-title":"Frontiers in molecular neuroscience"},{"issue":"2","key":"10.1016\/j.ebiom.2020.102825_bib0075","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1056\/NEJMoa1314768","article-title":"Dupilumab treatment in adults with moderate-to-severe atopic dermatitis","volume":"371","author":"Beck","year":"2014","journal-title":"The New England journal of medicine"},{"issue":"6","key":"10.1016\/j.ebiom.2020.102825_bib0076","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1080\/1744666X.2019.1601014","article-title":"Guselkumab for the treatment of adults with moderate to severe plaque psoriasis","volume":"15","author":"Puig","year":"2019","journal-title":"Expert Rev Clin Immunol"},{"issue":"5","key":"10.1016\/j.ebiom.2020.102825_bib0077","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1007\/s00401-018-1911-2","article-title":"Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau","volume":"136","author":"Courade","year":"2018","journal-title":"Acta neuropathologica"},{"key":"10.1016\/j.ebiom.2020.102825_bib0078","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/nrd.2018.138","article-title":"Therapeutic strategies targeting connexins","volume":"17","author":"Laird","year":"2018","journal-title":"Nat Rev Drug Discov"},{"issue":"12","key":"10.1016\/j.ebiom.2020.102825_bib0079","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/j.molmed.2018.10.005","article-title":"Therapeutic Targeting of Connexin Channels: New Views and Challenges","volume":"24","author":"Delvaeye","year":"2018","journal-title":"Trends in molecular medicine"},{"issue":"1","key":"10.1016\/j.ebiom.2020.102825_bib0080","doi-asserted-by":"crossref","first-page":"16623","DOI":"10.1038\/s41598-019-52900-4","article-title":"Blocking connexin43 hemichannels protects mice against tumour necrosis factor-induced inflammatory shock","volume":"9","author":"Delvaeye","year":"2019","journal-title":"Scientific reports"},{"issue":"8","key":"10.1016\/j.ebiom.2020.102825_bib0081","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1016\/j.febslet.2014.03.004","article-title":"Hunting for connexin hemichannels","volume":"588","author":"Saez","year":"2014","journal-title":"FEBS letters"},{"key":"10.1016\/j.ebiom.2020.102825_bib0082","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.neuropharm.2013.02.021","article-title":"Antibodies targeting extracellular domain of connexins for studies of hemichannels","volume":"75","author":"Riquelme","year":"2013","journal-title":"Neuropharmacology"},{"issue":"4","key":"10.1016\/j.ebiom.2020.102825_bib0083","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1007\/s10517-014-2603-0","article-title":"Treatment of Poorly Differentiated Glioma Using a Combination of Monoclonal Antibodies to Extracellular Connexin-43 Fragment, Temozolomide, and Radiotherapy","volume":"157","author":"Yusubalieva","year":"2014","journal-title":"B Exp Biol Med+"},{"key":"10.1016\/j.ebiom.2020.102825_bib0084","unstructured":"Jiang JX, An Z, Zhang N, et al., inventors; Connexin (cx) 43 hemichannel-binding antibodies and uses thereof. USA patent US 20190359696Al. 2019 Nov. 28, 2019."},{"issue":"8","key":"10.1016\/j.ebiom.2020.102825_bib0085","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nri.2016.67","article-title":"Combinatorial antibody libraries: new advances, new immunological insights","volume":"16","author":"Lerner","year":"2016","journal-title":"Nature reviews Immunology"},{"key":"10.1016\/j.ebiom.2020.102825_bib0086","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/978-1-62703-586-6_12","article-title":"Construction of human antibody gene libraries and selection of antibodies by phage display","volume":"1060","author":"Frenzel","year":"2014","journal-title":"Methods in molecular biology"},{"issue":"6301","key":"10.1016\/j.ebiom.2020.102825_bib0087","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1038\/348552a0","article-title":"Phage antibodies: filamentous phage displaying antibody variable domains","volume":"348","author":"McCafferty","year":"1990","journal-title":"Nature"},{"issue":"30","key":"10.1016\/j.ebiom.2020.102825_bib0088","doi-asserted-by":"crossref","first-page":"14971","DOI":"10.1073\/pnas.1908571116","article-title":"A cell-cell interaction format for selection of high-affinity antibodies to membrane proteins","volume":"116","author":"Yang","year":"2019","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"}],"container-title":["EBioMedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396420302000?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396420302000?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T19:32:00Z","timestamp":1778873520000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352396420302000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7]]},"references-count":88,"alternative-id":["S2352396420302000"],"URL":"https:\/\/doi.org\/10.1016\/j.ebiom.2020.102825","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.738172811.793580505","asserted-by":"object"}]},"ISSN":["2352-3964"],"issn-type":[{"value":"2352-3964","type":"print"}],"subject":[],"published":{"date-parts":[[2020,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A potent antagonist antibody targeting connexin hemichannels alleviates Clouston syndrome symptoms in mutant mice","name":"articletitle","label":"Article Title"},{"value":"EBioMedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ebiom.2020.102825","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"102825"}}