{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T13:04:44Z","timestamp":1781615084307,"version":"3.54.5"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T00:00:00Z","timestamp":1600128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004110","name":"Shanghai Normal University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004110","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["EBioMedicine"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.ebiom.2020.103036","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T01:44:06Z","timestamp":1602207846000},"page":"103036","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":39,"special_numbering":"C","title":["A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection"],"prefix":"10.1016","volume":"61","author":[{"given":"Weiren","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghua","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huailong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayong","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohui","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyi","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongliang","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liman","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yikun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5432-3225","authenticated-orcid":false,"given":"Bo","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renli","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuming","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dechang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoliang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanzheng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunying","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunshan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tieying","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuping","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wujiao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejiao","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huajun","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Weng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoping","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4008-9284","authenticated-orcid":false,"given":"Jin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"7798","key":"10.1016\/j.ebiom.2020.103036_bib0001","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/s41586-020-2008-3","article-title":"A new coronavirus associated with human respiratory disease in China","volume":"579","author":"Wu","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.ebiom.2020.103036_bib0002","series-title":"Cornavirus disease 2019 (COVID-19) Situation Report-131","author":"Organization","year":"2020"},{"issue":"3","key":"10.1016\/j.ebiom.2020.103036_bib0003","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","article-title":"Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR","volume":"25","author":"Corman","year":"2020","journal-title":"Euro Surveill"},{"issue":"5","key":"10.1016\/j.ebiom.2020.103036_bib0004","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s11604-020-00967-9","article-title":"A case report of COVID-19 with false negative RT-PCR test: necessity of chest CT","volume":"38","author":"Feng","year":"2020","journal-title":"Japan J Radiol"},{"key":"10.1016\/j.ebiom.2020.103036_bib0005","article-title":"Stability issues of RT-PCR testing of SARS-CoV-2 for hospitalized patients clinically diagnosed with COVID-19","author":"Li","year":"2020","journal-title":"J Med Virol"},{"issue":"6","key":"10.1016\/j.ebiom.2020.103036_bib0006","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1093\/clinchem\/hvaa091","article-title":"Potential false-negative nucleic acid testing results for severe acute respiratory syndrome coronavirus 2 from thermal inactivation of samples with low viral loads","volume":"66","author":"Pan","year":"2020","journal-title":"Clin Chem"},{"key":"10.1016\/j.ebiom.2020.103036_bib0007","doi-asserted-by":"crossref","DOI":"10.1016\/j.tmaid.2020.101673","article-title":"Detection and analysis of nucleic acid in various biological samples of COVID-19 patients","author":"Wu","year":"2020","journal-title":"Travel Med Infect Dis"},{"key":"10.1016\/j.ebiom.2020.103036_bib0008","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.25855","article-title":"False-negative of RT-PCR and prolonged nucleic acid conversion in COVID-19: Rather than recurrence","author":"Xiao","year":"2020","journal-title":"J Med Virol"},{"issue":"6336","key":"10.1016\/j.ebiom.2020.103036_bib0009","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"issue":"6387","key":"10.1016\/j.ebiom.2020.103036_bib0010","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"issue":"4","key":"10.1016\/j.ebiom.2020.103036_bib0011","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/s41422-018-0022-x","article-title":"CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA","volume":"28","author":"Li","year":"2018","journal-title":"Cell Res"},{"issue":"6387","key":"10.1016\/j.ebiom.2020.103036_bib0012","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"issue":"6416","key":"10.1016\/j.ebiom.2020.103036_bib0013","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1126\/science.aav4294","article-title":"Programmed DNA destruction by miniature CRISPR-Cas14 enzymes","volume":"362","author":"Harrington","year":"2018","journal-title":"Science"},{"issue":"10","key":"10.1016\/j.ebiom.2020.103036_bib0014","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1021\/acssynbio.9b00209","article-title":"HOLMESv2: A CRISPR-Cas12b-assisted platform for nucleic acid detection and DNA methylation quantitation","volume":"8","author":"Li","year":"2019","journal-title":"ACS Synth Biol"},{"issue":"6387","key":"10.1016\/j.ebiom.2020.103036_bib0015","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1126\/science.aat4982","article-title":"Next-generation diagnostics with CRISPR","volume":"360","author":"Chertow","year":"2018","journal-title":"Science"},{"issue":"39","key":"10.1016\/j.ebiom.2020.103036_bib0016","doi-asserted-by":"crossref","first-page":"E2579","DOI":"10.1073\/pnas.1208507109","article-title":"Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria","volume":"109","author":"Gasiunas","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6096","key":"10.1016\/j.ebiom.2020.103036_bib0017","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.ebiom.2020.103036_bib0018","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41421-018-0028-z","article-title":"CRISPR-Cas12a-assisted nucleic acid detection","volume":"4","author":"Li","year":"2018","journal-title":"Cell Discov"},{"issue":"4","key":"10.1016\/j.ebiom.2020.103036_bib0019","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat Methods"},{"key":"10.1016\/j.ebiom.2020.103036_bib0020","unstructured":"W.Z. Tieying Hou MY, Wenjing Chen, Lili Ren, Jingwen Ai, Ji Wu, Yalong Liao, Xuejing Gou, Yongjun Li, Xiaorui Wang, Hang Su, Bing Gu, Jianwei Wang, Teng Xu. Development and evaluation of A CRISPR-based diagnostic For 2019-novel coronavirus. medRxiv preprint2020; February 25, 2020. 10.1101\/2020.02.22.20025460."},{"key":"10.1016\/j.ebiom.2020.103036_bib0021","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bdq.2017.04.001","article-title":"Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)","volume":"12","author":"Forootan","year":"2017","journal-title":"Biomol Detect Quantif"},{"issue":"3","key":"10.1016\/j.ebiom.2020.103036_bib0022","first-page":"155","article-title":"Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction","volume":"15","author":"Bustin","year":"2004","journal-title":"J Biomol Techn JBT"},{"issue":"6","key":"10.1016\/j.ebiom.2020.103036_bib0023","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1007\/s00217-007-0683-z","article-title":"Modelling the limit of detection in real-time quantitative PCR","volume":"226","author":"Burns","year":"2007","journal-title":"Eur Food Res Technol"},{"key":"10.1016\/j.ebiom.2020.103036_bib0024","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.25968","article-title":"Letter to the editor: three cases of re-detectable positive SARS-CoV-2 RNA in recovered COVID-19 patients with antibodies","author":"Fu","year":"2020","journal-title":"J Med Virol"},{"issue":"1","key":"10.1016\/j.ebiom.2020.103036_bib0025","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1089\/adt.2011.438","article-title":"The evaluation of statistical methods for estimating the lower limit of detection","volume":"11","author":"Huang","year":"2013","journal-title":"ASSAY and Drug Dev Technol"},{"key":"10.1016\/j.ebiom.2020.103036_bib0026","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.jclinepi.2017.12.006","article-title":"Linear regression and the normality assumption","volume":"98","author":"Schmidt","year":"2018","journal-title":"J Clin Epidemiol"},{"issue":"2","key":"10.1016\/j.ebiom.2020.103036_bib0027","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.mam.2005.12.007","article-title":"The real-time polymerase chain reaction","volume":"27","author":"Kubista","year":"2006","journal-title":"Mol Asp Med"},{"key":"10.1016\/j.ebiom.2020.103036_bib0028","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.bdq.2015.07.001","article-title":"Removal of between-run variation in a multi-plate qPCR experiment","volume":"5","author":"Ruijter","year":"2015","journal-title":"Biomol Detect Quantif"}],"container-title":["EBioMedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396420304126?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396420304126?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T19:01:05Z","timestamp":1762455665000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352396420304126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":28,"alternative-id":["S2352396420304126"],"URL":"https:\/\/doi.org\/10.1016\/j.ebiom.2020.103036","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.06.02.20119735","asserted-by":"object"}]},"ISSN":["2352-3964"],"issn-type":[{"value":"2352-3964","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection","name":"articletitle","label":"Article Title"},{"value":"EBioMedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ebiom.2020.103036","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"103036"}}