{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:13:11Z","timestamp":1793661191227,"version":"4.5.1"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T00:00:00Z","timestamp":1620864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA016086"],"award-info":[{"award-number":["P30CA016086"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009915","name":"Mbarara University of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM007092"],"award-info":[{"award-number":["T32GM007092"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K24AI134990"],"award-info":[{"award-number":["K24AI134990"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI121558"],"award-info":[{"award-number":["R01AI121558"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI148579"],"award-info":[{"award-number":["R21AI148579"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001316","name":"Abbott Laboratories","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001316","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007878","name":"Diabetes Research Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007878","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["MRA-150G"],"award-info":[{"award-number":["MRA-150G"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["MRA-178"],"award-info":[{"award-number":["MRA-178"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["MRA-179"],"award-info":[{"award-number":["MRA-179"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["MRA-180"],"award-info":[{"award-number":["MRA-180"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["MRA-456G"],"award-info":[{"award-number":["MRA-456G"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["UL1TR002489"],"award-info":[{"award-number":["UL1TR002489"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016920","name":"Sari Agricultural Sciences and Natural Resources University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016920","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005564","name":"Gilead Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005564","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006808","name":"University of North Carolina","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006808","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004417","name":"Global Fund to Fight AIDS, Tuberculosis and Malaria","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004417","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004423","name":"World Health Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004423","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["EBioMedicine"],"published-print":{"date-parts":[[2021,6]]},"DOI":"10.1016\/j.ebiom.2021.103415","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T04:51:16Z","timestamp":1623646276000},"page":"103415","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":101,"special_numbering":"C","title":["A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping"],"prefix":"10.1016","volume":"68","author":[{"given":"Clark H.","family":"Cunningham","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Hennelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica T.","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratawan","family":"Ubalee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross M.","family":"Boyce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar M.","family":"Mulogo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Hathaway","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyaw L.","family":"Thwai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernandine","family":"Phanzu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Kalonji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kashamuka","family":"Mwandagalirwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoinette","family":"Tshefu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan J.","family":"Juliano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan B.","family":"Parr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ebiom.2021.103415_bib0001","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.4269\/ajtmh.17-0996","article-title":"Characterization of Plasmodium Lactate Dehydrogenase and Histidine-Rich Protein 2 Clearance Patterns via Rapid On-Bead Detection from a Single Dried Blood Spot","volume":"98","author":"Markwalter","year":"2018","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.ebiom.2021.103415_bib0002","doi-asserted-by":"crossref","first-page":"462","DOI":"10.3201\/eid2403.171723","article-title":"Major Threat to Malaria Control Programs by Plasmodium falciparum Lacking Histidine-Rich Protein 2, Eritrea","volume":"24","author":"Berhane","year":"2018","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.ebiom.2021.103415_bib0003","doi-asserted-by":"crossref","first-page":"5636","DOI":"10.1128\/JCM.42.12.5636-5643.2004","article-title":"Detection of Four Plasmodium Species in Blood from Humans by 18S rRNA Gene Subunit-Based and Species-Specific Real-Time PCR Assays","volume":"42","author":"Rougemont","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ebiom.2021.103415_bib0004","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01879-19","article-title":"Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections","volume":"58","author":"Lalremruata","year":"2020","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ebiom.2021.103415_bib0005","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1093\/infdis\/jit184","article-title":"Highly sensitive detection of malaria parasitemia in a malaria-endemic setting: Performance of a new loop-mediated isothermal amplification kit in a remote clinic in Uganda","volume":"208","author":"Hopkins","year":"2013","journal-title":"J Infect Dis"},{"key":"10.1016\/j.ebiom.2021.103415_bib0006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12936-018-2329-y","article-title":"A novel field-based molecular assay to detect validated artemisinin-resistant k13 mutants","volume":"17","author":"Vachot-Gan\u00e9e","year":"2018","journal-title":"Malar J"},{"key":"10.1016\/j.ebiom.2021.103415_bib0007","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1475-2875-11-23","article-title":"Development of a TaqMan Allelic Discrimination assay for detection of single nucleotides polymorphisms associated with anti-malarial drug resistance","volume":"11","author":"Kamau","year":"2012","journal-title":"Malar J"},{"key":"10.1016\/j.ebiom.2021.103415_bib0008","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofy011","article-title":"A Novel Single-Nucleotide Polymorphism Loop Mediated Isothermal Amplification Assay for Detection of Artemisinin-Resistant Plasmodium falciparum Malaria","volume":"5","author":"Mohon","year":"2018","journal-title":"Open Forum Infectious Diseases"},{"key":"10.1016\/j.ebiom.2021.103415_bib0009","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.ebiom.2021.103415_bib0010","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.ebiom.2021.103415_bib0011","doi-asserted-by":"crossref","unstructured":"Yuan C-Q, Tian T, Sun J, Hu M-L, Wang X-S, Xiong E-H, et\u00a0al. Universal and naked-eye gene detection platform based on CRISPR\/Cas12a\/13a system n.d. https:\/\/doi.org\/10.1101\/615724. 2021","DOI":"10.1101\/615724"},{"key":"10.1016\/j.ebiom.2021.103415_bib0012","doi-asserted-by":"crossref","first-page":"19702","DOI":"10.1038\/s41598-019-56170-y","article-title":"CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp","volume":"9","author":"Sullivan","year":"2019","journal-title":"Sci Rep"},{"key":"10.1016\/j.ebiom.2021.103415_bib0013","article-title":"Visual detection of porcine reproductive and respiratory syndrome virus using CRISPR-Cas13a","author":"Chang","year":"2019","journal-title":"Transbound Emerg Dis"},{"key":"10.1016\/j.ebiom.2021.103415_bib0014","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/S1473-3099(18)30732-1","article-title":"Effect of Plasmodium falciparum sulfadoxine-pyrimethamine resistance on the effectiveness of intermittent preventive therapy for malaria in pregnancy in Africa: a systematic review and meta-analysis","volume":"19","author":"van Eijk","year":"2019","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.ebiom.2021.103415_bib0015","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"10.1016\/j.ebiom.2021.103415_bib0016","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/S0020-7519(02)00111-X","article-title":"Three multigene families in Plasmodium parasites: facts and questions","volume":"32","author":"Mercereau-Puijalon","year":"2002","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.ebiom.2021.103415_bib0017","doi-asserted-by":"crossref","first-page":"6495","DOI":"10.1038\/s41598-021-85913-z","article-title":"Analysis of false-negative rapid diagnostic tests for symptomatic malaria in the Democratic Republic of the Congo","volume":"11","author":"Parr","year":"2021","journal-title":"Sci Rep"},{"key":"10.1016\/j.ebiom.2021.103415_bib0018","doi-asserted-by":"crossref","unstructured":"Boyce RM, Collins M, Muhindo R, Nakakande R, Ciccone EJ, Grounds SC, et\u00a0al. Dengue in western Uganda: A prospective cohort of children presenting with undifferentiated febrile illness. Infectious Diseases (except HIV\/AIDS) 2020. doi:10.1101\/2020.08.21.20179002.","DOI":"10.1101\/2020.08.21.20179002"},{"key":"10.1016\/j.ebiom.2021.103415_bib0019","doi-asserted-by":"crossref","first-page":"565","DOI":"10.4269\/ajtmh.1995.52.565","article-title":"Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa","volume":"52","author":"Plowe","year":"1995","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.ebiom.2021.103415_bib0020","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/s12936-021-03630-4","article-title":"Optimization of whole-genome sequencing of Plasmodium falciparum from low-density dried blood spot samples","volume":"20","author":"Teyssier","year":"2021","journal-title":"Malar J"},{"key":"10.1016\/j.ebiom.2021.103415_bib0021","doi-asserted-by":"crossref","first-page":"e71539","DOI":"10.1371\/journal.pone.0071539","article-title":"Multiplex qPCR for detection and absolute quantification of malaria","volume":"8","author":"Kamau","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ebiom.2021.103415_bib0022","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.exppara.2008.12.012","article-title":"Multiplex real-time PCR detection of P. falciparum, P. vivax and P. malariae in human blood samples","volume":"121","author":"Veron","year":"2009","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.ebiom.2021.103415_bib0023","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0175771","article-title":"Four human Plasmodium species quantification using droplet digital PCR","volume":"12","author":"Srisutham","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.ebiom.2021.103415_bib0024","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1128\/JCM.42.3.1214-1219.2004","article-title":"Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis","volume":"42","author":"Perandin","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ebiom.2021.103415_bib0025","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1186\/s12936-017-2110-7","article-title":"Individual and household characteristics of persons with Plasmodium falciparum malaria in sites with varying endemicities in Kinshasa Province, Democratic Republic of the Congo","volume":"16","author":"Mwandagalirwa","year":"2017","journal-title":"Malar J"},{"key":"10.1016\/j.ebiom.2021.103415_bib0026","doi-asserted-by":"crossref","first-page":"2418","DOI":"10.1128\/AAC.47.8.2418-2423.2003","article-title":"Resistance to Antimalarials in Southeast Asia and Genetic Polymorphisms in pfmdr1","volume":"47","author":"Pickard","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.ebiom.2021.103415_bib0027","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1093\/infdis\/jiv599","article-title":"Microscopic Plasmodium falciparum gametocytemia and infectivity to mosquitoes in Cambodia","volume":"213","author":"Lin","year":"2015","journal-title":"J Infect Dis"},{"key":"10.1016\/j.ebiom.2021.103415_bib0028","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1093\/nar\/gkx1201","article-title":"SeekDeep: single-base resolution de novo clustering for amplicon deep sequencing","volume":"46","author":"Hathaway","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.ebiom.2021.103415_bib0029","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1186\/1475-2875-12-277","article-title":"Comparative evaluation of published real-time PCR assays for the detection of malaria following MIQE guidelines","volume":"12","author":"Alemayehu","year":"2013","journal-title":"Malar J"},{"key":"10.1016\/j.ebiom.2021.103415_bib0030","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00565-18","article-title":"Operational Performance of a Plasmodium falciparum Ultrasensitive Rapid Diagnostic Test for Detection of Asymptomatic Infections in Eastern Myanmar","volume":"56","author":"Landier","year":"2018","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ebiom.2021.103415_bib0031","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/s12936-018-2268-7","article-title":"Performance of an ultra-sensitive Plasmodium falciparum HRP2-based rapid diagnostic test with recombinant HRP2, culture parasites, and archived whole blood samples","volume":"17","author":"Das","year":"2018","journal-title":"Malar J"},{"key":"10.1016\/j.ebiom.2021.103415_bib0032","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.1001788","article-title":"Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets","volume":"12","author":"Hofmann","year":"2015","journal-title":"PLoS Med"},{"key":"10.1016\/j.ebiom.2021.103415_bib0033","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1128\/JCM.01057-14","article-title":"High-throughput ultrasensitive molecular techniques for quantifying low-density malaria parasitemias","volume":"52","author":"Imwong","year":"2014","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ebiom.2021.103415_bib0034","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1093\/infdis\/jiu252","article-title":"Field evaluation of a real-time fluorescence loop-mediated isothermal amplification assay, RealAmp, for the diagnosis of malaria in Thailand and India","volume":"210","author":"Patel","year":"2014","journal-title":"J Infect Dis"},{"key":"10.1016\/j.ebiom.2021.103415_bib0035","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1056\/NEJMcibr1704902","article-title":"A CRISPR Way to Diagnose Infectious Diseases","volume":"377","author":"Caliendo","year":"2017","journal-title":"N Engl J Med"},{"key":"10.1016\/j.ebiom.2021.103415_bib0036","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1017\/S0031182017001329","article-title":"PacBio assembly of a Plasmodium knowlesi genome sequence with Hi-C correction and manual annotation of the SICAvar gene family","volume":"145","author":"Lapp","year":"2018","journal-title":"Parasitology"},{"key":"10.1016\/j.ebiom.2021.103415_bib0037","doi-asserted-by":"crossref","first-page":"42","DOI":"10.12688\/wellcomeopenres.16168.1","article-title":"An open dataset of Plasmodium falciparum genome variation in 7,000 worldwide samples","volume":"6","author":"MalariaGEN","year":"2021","journal-title":"Wellcome Open Res"},{"key":"10.1016\/j.ebiom.2021.103415_bib0038","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1093\/infdis\/jiu836","article-title":"The A581 G mutation in the gene encoding Plasmodium falciparum dihydropteroate synthetase reduces the effectiveness of sulfadoxine-pyrimethamine preventive therapy in Malawian pregnant women","volume":"211","author":"Gutman","year":"2015","journal-title":"J Infect Dis"},{"key":"10.1016\/j.ebiom.2021.103415_bib0039","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S0140-6736(13)60024-0","article-title":"Malaria","volume":"383","author":"White","year":"2014","journal-title":"Lancet North Am Ed"},{"key":"10.1016\/j.ebiom.2021.103415_bib0040","doi-asserted-by":"crossref","first-page":"25722","DOI":"10.1073\/pnas.2010196117","article-title":"Ultrasensitive CRISPR-based diagnostic for field-applicable detection of Plasmodium species in symptomatic and asymptomatic malaria","volume":"117","author":"Lee","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.ebiom.2021.103415_bib0041","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.ebiom.2021.103415_bib0042","doi-asserted-by":"crossref","first-page":"aaf5573","DOI":"10.1126\/science.aaf5573","article-title":"C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector","volume":"353","author":"Abudayyeh","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.ebiom.2021.103415_bib0043","doi-asserted-by":"crossref","first-page":"2986","DOI":"10.1038\/s41596-019-0210-2","article-title":"SHERLOCK: nucleic acid detection with CRISPR nucleases","volume":"14","author":"Kellner","year":"2019","journal-title":"Nat Protoc"},{"key":"10.1016\/j.ebiom.2021.103415_bib0044","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2020.12.001","article-title":"Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy","volume":"184","author":"Fozouni","year":"2021","journal-title":"Cell"}],"container-title":["EBioMedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396421002085?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396421002085?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T19:06:08Z","timestamp":1762455968000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352396421002085"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6]]},"references-count":44,"alternative-id":["S2352396421002085"],"URL":"https:\/\/doi.org\/10.1016\/j.ebiom.2021.103415","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.04.01.017962","asserted-by":"object"}]},"ISSN":["2352-3964"],"issn-type":[{"value":"2352-3964","type":"print"}],"subject":[],"published":{"date-parts":[[2021,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping","name":"articletitle","label":"Article Title"},{"value":"EBioMedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ebiom.2021.103415","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"103415"}}