{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T06:52:06Z","timestamp":1793429526062,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004739","name":"Chinese Academy of Sciences Youth Innovation Promotion Association","doi-asserted-by":"publisher","award":["Y2022037"],"award-info":[{"award-number":["Y2022037"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012492","name":"Youth Innovation Promotion Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021831","name":"Institute of Microbiology, Chinese Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021831","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82225021"],"award-info":[{"award-number":["82225021"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171428"],"award-info":[{"award-number":["32171428"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["eBioMedicine"],"published-print":{"date-parts":[[2024,11]]},"DOI":"10.1016\/j.ebiom.2024.105392","type":"journal-article","created":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T21:33:52Z","timestamp":1729200832000},"page":"105392","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"special_numbering":"C","title":["Single-chain A35R-M1R-B6R trivalent mRNA vaccines protect mice against both mpox virus and vaccinia virus"],"prefix":"10.1016","volume":"109","author":[{"given":"Tianxiang","family":"Kong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6152-9050","authenticated-orcid":false,"given":"Renyi","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuehui","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengrong","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanlang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5076-3215","authenticated-orcid":false,"given":"Zejun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qihui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3869-615X","authenticated-orcid":false,"given":"George F.","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.ebiom.2024.105392_bib1","first-page":"165","article-title":"Human monkeypox, 1970-79","volume":"58","author":"Breman","year":"1980","journal-title":"Bull World Health Organ"},{"issue":"2","key":"10.1016\/j.ebiom.2024.105392_bib2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010141","article-title":"The changing epidemiology of human monkeypox-A potential threat? A systematic review","volume":"16","author":"Bunge","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"10.1016\/j.ebiom.2024.105392_bib3","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.medmal.2018.02.010","article-title":"Investigation of an outbreak of monkeypox in an area occupied by armed groups, Central African Republic","volume":"48","author":"Kalthan","year":"2018","journal-title":"Med Mal Infect"},{"issue":"38","key":"10.1016\/j.ebiom.2024.105392_bib4","first-page":"847","article-title":"Neglected zoonotic monkeypox in Africa but now back in the spotlight worldwide","volume":"4","author":"Tan","year":"2022","journal-title":"China CDC Wkly"},{"issue":"38","key":"10.1016\/j.ebiom.2024.105392_bib5","first-page":"853","article-title":"The first imported case of monkeypox in the mainland of China - chongqing Municipality, China, September 16, 2022","volume":"4","author":"Zhao","year":"2022","journal-title":"China CDC Wkly"},{"key":"10.1016\/j.ebiom.2024.105392_bib6","series-title":"Medical microbiology. Galveston (TX)","author":"Baxby","year":"1996"},{"issue":"Pt 11","key":"10.1016\/j.ebiom.2024.105392_bib7","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1099\/vir.0.055921-0","article-title":"Vaccinia virus immune evasion: mechanisms, virulence and immunogenicity","volume":"94","author":"Smith","year":"2013","journal-title":"J\u00a0Gen Virol"},{"issue":"22","key":"10.1016\/j.ebiom.2024.105392_bib9","doi-asserted-by":"crossref","first-page":"734","DOI":"10.15585\/mmwr.mm7122e1","article-title":"Use of JYNNEOS (smallpox and monkeypox vaccine, live, nonreplicating) for preexposure vaccination of persons at risk for occupational exposure to orthopoxviruses: recommendations of the advisory committee on immunization practices - United States, 2022","volume":"71","author":"Rao","year":"2022","journal-title":"MMWR Morb Mortal Wkly Rep"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib10","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/s41541-022-00527-4","article-title":"Current status of monkeypox vaccines","volume":"7","author":"Gruber","year":"2022","journal-title":"NPJ Vaccines"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib11","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41591-022-02090-w","article-title":"Low levels of monkeypox virus-neutralizing antibodies after MVA-BN vaccination in healthy individuals","volume":"29","author":"Zaeck","year":"2023","journal-title":"Nat Med"},{"issue":"6","key":"10.1016\/j.ebiom.2024.105392_bib12","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/j.chom.2023.05.001","article-title":"Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals","volume":"31","author":"Hubert","year":"2023","journal-title":"Cell Host Microbe"},{"issue":"26","key":"10.1016\/j.ebiom.2024.105392_bib13","doi-asserted-by":"crossref","first-page":"2434","DOI":"10.1056\/NEJMoa2215201","article-title":"Vaccine effectiveness of JYNNEOS against mpox disease in the United States","volume":"388","author":"Deputy","year":"2023","journal-title":"N\u00a0Engl J Med"},{"issue":"10","key":"10.1016\/j.ebiom.2024.105392_bib14","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.tim.2008.07.009","article-title":"Vaccinia virus morphogenesis and dissemination","volume":"16","author":"Roberts","year":"2008","journal-title":"Trends Microbiol"},{"issue":"25-26","key":"10.1016\/j.ebiom.2024.105392_bib15","doi-asserted-by":"crossref","first-page":"3358","DOI":"10.1016\/j.vaccine.2004.02.034","article-title":"Differential efficacy of vaccinia virus envelope proteins administered by DNA immunisation in protection of BALB\/c mice from a lethal intranasal poxvirus challenge","volume":"22","author":"Pulford","year":"2004","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib16","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1006\/viro.1998.9516","article-title":"Neutralizing and protective antibodies directed against vaccinia virus envelope antigens","volume":"254","author":"Galmiche","year":"1999","journal-title":"Virology"},{"issue":"3","key":"10.1016\/j.ebiom.2024.105392_bib17","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.cell.2016.09.049","article-title":"Cross-neutralizing and protective human antibody specificities to poxvirus infections","volume":"167","author":"Gilchuk","year":"2016","journal-title":"Cell"},{"issue":"4","key":"10.1016\/j.ebiom.2024.105392_bib18","doi-asserted-by":"crossref","first-page":"2552","DOI":"10.4049\/jimmunol.177.4.2552","article-title":"Subunit recombinant vaccine protects against monkeypox","volume":"177","author":"Heraud","year":"2006","journal-title":"J\u00a0Immunol"},{"issue":"2","key":"10.1016\/j.ebiom.2024.105392_bib19","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.vaccine.2012.11.007","article-title":"Adsorption of recombinant poxvirus L1-protein to aluminum hydroxide\/CpG vaccine adjuvants enhances immune responses and protection of mice from vaccinia virus challenge","volume":"31","author":"Xiao","year":"2013","journal-title":"Vaccine"},{"issue":"19","key":"10.1016\/j.ebiom.2024.105392_bib20","doi-asserted-by":"crossref","first-page":"10230","DOI":"10.1128\/JVI.78.19.10230-10237.2004","article-title":"Protective immunity to vaccinia virus induced by vaccination with multiple recombinant outer membrane proteins of intracellular and extracellular virions","volume":"78","author":"Fogg","year":"2004","journal-title":"J\u00a0Virol"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib21","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0042-6822(02)00038-7","article-title":"Four-gene-combination DNA vaccine protects mice against a lethal vaccinia virus challenge and elicits appropriate antibody responses in nonhuman primates","volume":"306","author":"Hooper","year":"2003","journal-title":"Virology"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib22","doi-asserted-by":"crossref","DOI":"10.1080\/22221751.2023.2204151","article-title":"Mpox multi-antigen mRNA vaccine candidates by a simplified manufacturing strategy afford efficient protection against lethal orthopoxvirus challenge","volume":"12","author":"Zeng","year":"2023","journal-title":"Emerg Microbes Infect"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib23","doi-asserted-by":"crossref","DOI":"10.1080\/22221751.2023.2192815","article-title":"Rational development of multicomponent mRNA vaccine candidates against mpox","volume":"12","author":"Zhang","year":"2023","journal-title":"Emerg Microbes Infect"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib24","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1038\/s41392-023-01432-5","article-title":"Monkeypox virus quadrivalent mRNA vaccine induces immune response and protects against vaccinia virus","volume":"8","author":"Sang","year":"2023","journal-title":"Signal Transduct Target Ther"},{"key":"10.1016\/j.ebiom.2024.105392_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2023.105668","article-title":"Mpox virus mRNA-lipid nanoparticle vaccine candidates evoke antibody responses and drive protection against the Vaccinia virus challenge in mice","volume":"216","author":"Xia","year":"2023","journal-title":"Antiviral Res"},{"issue":"6","key":"10.1016\/j.ebiom.2024.105392_bib26","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1016\/j.cell.2024.01.017","article-title":"A\u00a0multivalent mRNA monkeypox virus vaccine (BNT166) protects mice and macaques from orthopoxvirus disease","volume":"187","author":"Zuiani","year":"2024","journal-title":"Cell"},{"issue":"716","key":"10.1016\/j.ebiom.2024.105392_bib27","doi-asserted-by":"crossref","first-page":"eadg3540","DOI":"10.1126\/scitranslmed.adg3540","article-title":"An mpox virus mRNA-lipid nanoparticle vaccine confers protection against lethal orthopoxviral challenge","volume":"15","author":"Freyn","year":"2023","journal-title":"Sci Transl Med"},{"issue":"5","key":"10.1016\/j.ebiom.2024.105392_bib28","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/s41422-023-00792-5","article-title":"Polyvalent mRNA vaccination elicited potent immune response to monkeypox virus surface antigens","volume":"33","author":"Fang","year":"2023","journal-title":"Cell Res"},{"issue":"4","key":"10.1016\/j.ebiom.2024.105392_bib29","doi-asserted-by":"crossref","first-page":"385","DOI":"10.3390\/vaccines12040385","article-title":"A\u00a0quadrivalent mRNA immunization elicits potent immune responses against multiple orthopoxviral antigens and neutralization of monkeypox virus in rodent models","volume":"12","author":"Su","year":"2024","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.ebiom.2024.105392_bib30","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1203410","article-title":"Recombinant proteins A29L, M1R, A35R, and B6R vaccination protects mice from mpox virus challenge","volume":"14","author":"Tang","year":"2023","journal-title":"Front Immunol"},{"issue":"2","key":"10.1016\/j.ebiom.2024.105392_bib31","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1038\/s41590-023-01715-7","article-title":"Rational design of a 'two-in-one' immunogen DAM drives potent immune response against mpox virus","volume":"25","author":"Wang","year":"2024","journal-title":"Nat Immunol"},{"issue":"11","key":"10.1016\/j.ebiom.2024.105392_bib32","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1038\/s41422-022-00720-z","article-title":"mRNA vaccines expressing homo-prototype\/Omicron and hetero-chimeric RBD-dimers against SARS-CoV-2","volume":"32","author":"Han","year":"2022","journal-title":"Cell Res"},{"issue":"9","key":"10.1016\/j.ebiom.2024.105392_bib33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005148","article-title":"Structural and functional characterization of anti-A33 antibodies reveal a potent cross-species orthopoxviruses neutralizer","volume":"11","author":"Matho","year":"2015","journal-title":"PLoS Pathog"},{"issue":"3","key":"10.1016\/j.ebiom.2024.105392_bib34","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1016\/j.cell.2020.06.035","article-title":"A\u00a0universal design of betacoronavirus vaccines against COVID-19, MERS, and SARS","volume":"182","author":"Dai","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.ebiom.2024.105392_bib35","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.jviromet.2013.10.026","article-title":"Development and comparison of a quantitative TaqMan-MGB real-time PCR assay to three other methods of quantifying vaccinia virions","volume":"196","author":"Baker","year":"2014","journal-title":"J\u00a0Virol Methods"},{"key":"10.1016\/j.ebiom.2024.105392_bib36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11427-023-2378-x","article-title":"Multi-valent mRNA vaccines against monkeypox enveloped or mature viron surface antigens demonstrate robust immune response and neutralizing activity","volume":"66","author":"Zhang","year":"2023","journal-title":"Sci China Life Sci"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib37","doi-asserted-by":"crossref","first-page":"5925","DOI":"10.1038\/s41467-023-41628-5","article-title":"mRNA vaccines encoding fusion proteins of monkeypox virus antigens protect mice from vaccinia virus challenge","volume":"14","author":"Hou","year":"2023","journal-title":"Nat Commun"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib38","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1080\/22221751.2022.2056524","article-title":"A\u00a0tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primates","volume":"11","author":"An","year":"2022","journal-title":"Emerg Microbes Infect"},{"issue":"13","key":"10.1016\/j.ebiom.2024.105392_bib39","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1016\/j.cell.2022.04.029","article-title":"Protective prototype-beta and delta-omicron chimeric RBD-dimer vaccines against SARS-CoV-2","volume":"185","author":"Xu","year":"2022","journal-title":"Cell"},{"issue":"23","key":"10.1016\/j.ebiom.2024.105392_bib40","doi-asserted-by":"crossref","first-page":"3003","DOI":"10.1016\/j.scib.2023.09.048","article-title":"Classification of five SARS-CoV-2 serotypes based on RBD antigenicities","volume":"68","author":"Hu","year":"2023","journal-title":"Sci Bull (Beijing)"},{"issue":"7","key":"10.1016\/j.ebiom.2024.105392_bib41","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.scib.2023.03.027","article-title":"A\u00a0broadly neutralizing nanobody targeting the highly conserved S2 subunit of sarbecoviruses","volume":"68","author":"Wang","year":"2023","journal-title":"Sci Bull (Beijing)"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib42","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.hlife.2023.10.002","article-title":"Efficient inhibition of SARS-CoV-2 emerging EG.5, EG.5.1 and BA.2.86 variants by fusion inhibitor HY3000 peptide","volume":"2","author":"Wu","year":"2024","journal-title":"hLife"},{"issue":"1","key":"10.1016\/j.ebiom.2024.105392_bib43","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1128\/JVI.72.1.294-302.1998","article-title":"Functional analysis of vaccinia virus B5R protein: essential role in virus envelopment is independent of a large portion of the extracellular domain","volume":"72","author":"Herrera","year":"1998","journal-title":"J\u00a0Virol"},{"issue":"12","key":"10.1016\/j.ebiom.2024.105392_bib44","doi-asserted-by":"crossref","first-page":"4240","DOI":"10.1073\/pnas.0501103102","article-title":"The 1.51-Angstrom structure of the poxvirus L1 protein, a target of potent neutralizing antibodies","volume":"102","author":"Su","year":"2005","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["eBioMedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396424004286?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396424004286?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T19:38:33Z","timestamp":1732045113000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352396424004286"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":43,"alternative-id":["S2352396424004286"],"URL":"https:\/\/doi.org\/10.1016\/j.ebiom.2024.105392","relation":{},"ISSN":["2352-3964"],"issn-type":[{"value":"2352-3964","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-chain A35R-M1R-B6R trivalent mRNA vaccines protect mice against both mpox virus and vaccinia virus","name":"articletitle","label":"Article Title"},{"value":"eBioMedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ebiom.2024.105392","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"105392"}}