{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:19:44Z","timestamp":1791515984851,"version":"4.3.4"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1300901"],"award-info":[{"award-number":["2021YFA1300901"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["eBioMedicine"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.ebiom.2025.105776","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T09:52:51Z","timestamp":1748598771000},"page":"105776","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Diverse impacts of different rpoB mutations on the anti-tuberculosis efficacy of capreomycin"],"prefix":"10.1016","volume":"117","author":[{"given":"Jin-Tian","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3229-0108","authenticated-orcid":false,"given":"Yao-Kai","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Fang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao-Yu","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ebiom.2025.105776_bib1","series-title":"Global tuberculosis report 2022","year":"2022"},{"issue":"3","key":"10.1016\/j.ebiom.2025.105776_bib2","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/0005-2787(67)90147-5","article-title":"The specific inhibition of the DNA-directed RNA synthesis by rifamycin","volume":"145","author":"Hartmann","year":"1967","journal-title":"Biochim Biophys Acta"},{"issue":"2","key":"10.1016\/j.ebiom.2025.105776_bib3","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1021\/cr030112j","article-title":"Rifamycin-mode of action, resistance, and biosynthesis","volume":"105","author":"Floss","year":"2005","journal-title":"Chem Rev"},{"issue":"8846","key":"10.1016\/j.ebiom.2025.105776_bib4","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/0140-6736(93)90417-F","article-title":"Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis","volume":"341","author":"Telenti","year":"1993","journal-title":"Lancet"},{"issue":"4","key":"10.1016\/j.ebiom.2025.105776_bib5","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1128\/CMR.8.4.496","article-title":"Antimicrobial agent resistance in mycobacteria: molecular genetic insights","volume":"8","author":"Musser","year":"1995","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"10.1016\/j.ebiom.2025.105776_bib6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1054\/tuld.1998.0002","article-title":"Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update","volume":"79","author":"Ramaswamy","year":"1998","journal-title":"Tuber Lung Dis"},{"issue":"4","key":"10.1016\/j.ebiom.2025.105776_bib7","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1128\/jcm.32.4.1095-1098.1994","article-title":"Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas","volume":"32","author":"Kapur","year":"1994","journal-title":"J Clin Microbiol"},{"issue":"10","key":"10.1016\/j.ebiom.2025.105776_bib8","doi-asserted-by":"crossref","first-page":"2380","DOI":"10.1128\/AAC.38.10.2380","article-title":"Characterization of rifampin-resistance in pathogenic mycobacteria","volume":"38","author":"Williams","year":"1994","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"10.1016\/j.ebiom.2025.105776_bib9","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1038\/ng.2735","article-title":"Genome sequencing of 161 Mycobacterium tuberculosis isolates from China identifies genes and intergenic regions associated with drug resistance","volume":"45","author":"Zhang","year":"2013","journal-title":"Nat Genet"},{"issue":"2","key":"10.1016\/j.ebiom.2025.105776_bib10","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1038\/s41588-017-0029-0","article-title":"Genome-wide analysis of multi- and extensively drug-resistant Mycobacterium tuberculosis","volume":"50","author":"Coll","year":"2018","journal-title":"Nat Genet"},{"issue":"9","key":"10.1016\/j.ebiom.2025.105776_bib11","doi-asserted-by":"crossref","first-page":"e672","DOI":"10.1016\/S2666-5247(22)00116-1","article-title":"Investigating resistance in clinical Mycobacterium tuberculosis complex isolates with genomic and phenotypic antimicrobial susceptibility testing: a multicentre observational study","volume":"3","author":"Finci","year":"2022","journal-title":"Lancet Microbe"},{"issue":"2","key":"10.1016\/j.ebiom.2025.105776_bib12","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1111\/j.1749-6632.1966.tb45543.x","article-title":"Capreomycin in the treatment of pulmonary tuberculosis","volume":"135","author":"Donomae","year":"1966","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.ebiom.2025.105776_bib13","series-title":"Guidelines for the management of drug-resistant tuberculosis","year":"1997"},{"issue":"3","key":"10.1016\/j.ebiom.2025.105776_bib14","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1038\/nsmb.1755","article-title":"The structures of the anti-tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome","volume":"17","author":"Stanley","year":"2010","journal-title":"Nat Struct Mol Biol"},{"issue":"19","key":"10.1016\/j.ebiom.2025.105776_bib15","doi-asserted-by":"crossref","first-page":"10271","DOI":"10.1073\/pnas.2002888117","article-title":"The structural basis for inhibition of ribosomal translocation by viomycin","volume":"117","author":"Zhang","year":"2020","journal-title":"Proc Natl Acad Sci USA"},{"issue":"2","key":"10.1016\/j.ebiom.2025.105776_bib16","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.molcel.2006.05.044","article-title":"Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2\u2019-O-methylations in 16S and 23S rRNAs","volume":"23","author":"Johansen","year":"2006","journal-title":"Mol Cell"},{"issue":"2","key":"10.1016\/j.ebiom.2025.105776_bib17","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1128\/AAC.49.2.571-577.2005","article-title":"Mutation of tlyA confers capreomycin resistance in Mycobacterium tuberculosis","volume":"49","author":"Maus","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"10.1016\/j.ebiom.2025.105776_bib18","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.diagmicrobio.2013.06.031","article-title":"Mycobacterium tuberculosis rrs A1401G mutation correlates with high-level resistance to kanamycin, amikacin, and capreomycin in clinical isolates from mainland China","volume":"77","author":"Du","year":"2013","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"7","key":"10.1016\/j.ebiom.2025.105776_bib19","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.1128\/AAC.00222-16","article-title":"Frequency and distribution of tuberculosis resistance-associated mutations between Mumbai, Moldova, and Eastern Cape","volume":"60","author":"Georghiou","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.ebiom.2025.105776_bib20","series-title":"WHO treatment guidelines for drug-resistant tuberculosis, 2016 update","year":"2016"},{"issue":"10150","key":"10.1016\/j.ebiom.2025.105776_bib21","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/S0140-6736(18)31644-1","article-title":"Treatment correlates of successful outcomes in pulmonary multidrug-resistant tuberculosis: an individual patient data meta-analysis","volume":"392","author":"Ahmad","year":"2018","journal-title":"Lancet"},{"issue":"11","key":"10.1016\/j.ebiom.2025.105776_bib22","doi-asserted-by":"crossref","first-page":"e3929","DOI":"10.1093\/cid\/ciaa621","article-title":"Aminoglycosides and capreomycin in the treatment of multidrug-resistant tuberculosis: individual patient data meta-analysis of 12 030 patients from 25 countries, 2009-2016","volume":"73","author":"Cegielski","year":"2021","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.ebiom.2025.105776_bib23","series-title":"Rapid Communication: Key changes to treatment of multidrug-and rifampicin-resistant tuberculosis (MDR\/RR-TB)","year":"2018"},{"issue":"5","key":"10.1016\/j.ebiom.2025.105776_bib24","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1378\/chest.111.5.1162","article-title":"Retreatment tuberculosis cases. Factors associated with drug resistance and adverse outcomes","volume":"111","author":"Kritski","year":"1997","journal-title":"Chest"},{"issue":"4","key":"10.1016\/j.ebiom.2025.105776_bib25","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1183\/09031936.00168008","article-title":"Epidemiology and clinical management of XDR-TB: a systematic review by TBNET","volume":"33","author":"Sotgiu","year":"2009","journal-title":"Eur Respir J"},{"issue":"10","key":"10.1016\/j.ebiom.2025.105776_bib26","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.1021\/acs.accounts.0c00878","article-title":"Strategies to combat multi-drug resistance in tuberculosis","volume":"54","author":"Singh","year":"2021","journal-title":"Acc Chem Res"},{"issue":"2","key":"10.1016\/j.ebiom.2025.105776_bib27","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1093\/jac\/dku384","article-title":"Evaluation of the microscopic observation drug susceptibility assay for the rapid detection of MDR-TB and XDR-TB in China: a prospective multicentre study","volume":"70","author":"Huang","year":"2015","journal-title":"J Antimicrob Chemother"},{"issue":"1","key":"10.1016\/j.ebiom.2025.105776_bib28","doi-asserted-by":"crossref","first-page":"96","DOI":"10.7705\/biomedica.v37i2.3204","article-title":"[Prevalence of Mycobacterium tuberculosis resistance to quinolones and injectables in Colombia, 2012-2013]","volume":"37","author":"Llerena","year":"2017","journal-title":"Biomedica"},{"issue":"3","key":"10.1016\/j.ebiom.2025.105776_bib29","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1128\/JCM.01865-16","article-title":"Evaluation of the GenoType MTBDRsl version 2.0 assay for second-line drug resistance detection of Mycobacterium tuberculosis isolates in South Africa","volume":"55","author":"Gardee","year":"2017","journal-title":"J Clin Microbiol"},{"issue":"7","key":"10.1016\/j.ebiom.2025.105776_bib30","doi-asserted-by":"crossref","first-page":"2680","DOI":"10.1128\/JCM.02144-10","article-title":"Impact of rpoB mutations on reduced vancomycin susceptibility in Staphylococcus aureus","volume":"49","author":"Watanabe","year":"2011","journal-title":"J Clin Microbiol"},{"issue":"204","key":"10.1016\/j.ebiom.2025.105776_bib31","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3006609","article-title":"Use of collateral sensitivity networks to design drug cycling protocols that avoid resistance development","volume":"5","author":"Imamovic","year":"2013","journal-title":"Sci Transl Med"},{"issue":"10","key":"10.1016\/j.ebiom.2025.105776_bib32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3000515","article-title":"Pervasive and diverse collateral sensitivity profiles inform optimal strategies to limit antibiotic resistance","volume":"17","author":"Maltas","year":"2019","journal-title":"PLoS Biol"},{"issue":"3","key":"10.1016\/j.ebiom.2025.105776_bib33","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1128\/JCM.41.3.1235-1239.2003","article-title":"Susceptibility testing with the manual mycobacteria growth indicator tube (MGIT) and the MGIT 960 system provides rapid and reliable verification of multidrug-resistant tuberculosis","volume":"41","author":"Adjers-Koskela","year":"2003","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.ebiom.2025.105776_bib34","doi-asserted-by":"crossref","DOI":"10.1002\/jcla.22184","article-title":"Identification of nontuberculous mycobacteria using multilocous sequence analysis of 16S rRNA, hsp65, and rpoB","volume":"32","author":"Kim","year":"2018","journal-title":"J Clin Lab Anal"},{"key":"10.1016\/j.ebiom.2025.105776_bib35","series-title":"Policy guidance on drug-susceptibility testing (DST) of second-line antituberculosis drugs","article-title":"WHO guidelines approved by the guidelines review committee","author":"Barrera","year":"2008"},{"issue":"1","key":"10.1016\/j.ebiom.2025.105776_bib36","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.01465-21","article-title":"Decreased methylenetetrahydrofolate reductase activity leads to increased sensitivity to para-aminosalicylic acid in Mycobacterium tuberculosis","volume":"66","author":"Yu","year":"2022","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"10.1016\/j.ebiom.2025.105776_bib37","doi-asserted-by":"crossref","DOI":"10.1080\/22221751.2024.2374030","article-title":"The T120P or M172V mutation on rv2172c confers high level para-aminosalicylic acid resistance in Mycobacterium tuberculosis","volume":"13","author":"Xu","year":"2024","journal-title":"Emerg Microbes Infect"},{"key":"10.1016\/j.ebiom.2025.105776_bib38","series-title":"A quality control tool for high throughput sequence data","year":"2022"},{"issue":"17","key":"10.1016\/j.ebiom.2025.105776_bib39","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"issue":"3","key":"10.1016\/j.ebiom.2025.105776_bib41","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gku1196","article-title":"Rapid phylogenetic analysis of large samples of recombinant bacterial whole genome sequences using Gubbins","volume":"43","author":"Croucher","year":"2015","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"10.1016\/j.ebiom.2025.105776_bib42","article-title":"SNP-sites: rapid efficient extraction of SNPs from multi-FASTA alignments","volume":"2","author":"Page","year":"2016","journal-title":"Microb Genom"},{"issue":"17","key":"10.1016\/j.ebiom.2025.105776_bib43","doi-asserted-by":"crossref","first-page":"4658","DOI":"10.1093\/bioinformatics\/btaa582","article-title":"Very Fast Tree: speeding up the estimation of phylogenies for large alignments through parallelization and vectorization strategies","volume":"36","author":"Pi\u00f1eiro","year":"2020","journal-title":"Bioinformatics"},{"issue":"2","key":"10.1016\/j.ebiom.2025.105776_bib44","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1005958","article-title":"A phylogenetic method to perform genome-wide association studies in microbes that accounts for population structure and recombination","volume":"14","author":"Collins","year":"2018","journal-title":"PLoS Comput Biol"},{"key":"10.1016\/j.ebiom.2025.105776_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.tube.2024.102551","article-title":"The rv2820c K114N mutation is related with capreomycin tolerance","volume":"148","author":"Xu","year":"2024","journal-title":"Tuberculosis (Edinb)"},{"key":"10.1016\/j.ebiom.2025.105776_bib46","series-title":"Meeting report of the WHO expert consultation on the definition of extensively drug-resistant tuberculosis","year":"2020"},{"issue":"6327","key":"10.1016\/j.ebiom.2025.105776_bib47","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1126\/science.aaj2191","article-title":"Antibiotic tolerance facilitates the evolution of resistance","volume":"355","author":"Levin-Reisman","year":"2017","journal-title":"Science"},{"issue":"12","key":"10.1016\/j.ebiom.2025.105776_bib48","article-title":"Whole genome sequencing reveals large deletions and other loss of function mutations in Mycobacterium tuberculosis drug resistance genes","volume":"7","author":"Gomes","year":"2021","journal-title":"Microb Genom"},{"key":"10.1016\/j.ebiom.2025.105776_bib49","first-page":"712","article-title":"Collateral susceptibility of isoniazid-resistant tubercle bacilli to nitrofurans","volume":"88","author":"Beutner","year":"1963","journal-title":"Am Rev Respir Dis"},{"key":"10.1016\/j.ebiom.2025.105776_bib50","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.ebiom.2016.05.041","article-title":"Paradoxical hypersusceptibility of drug-resistant Mycobacterium tuberculosis to \u03b2-lactam antibiotics","volume":"9","author":"Cohen","year":"2016","journal-title":"EBioMedicine"},{"issue":"1","key":"10.1016\/j.ebiom.2025.105776_bib51","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1038\/s41467-023-37184-7","article-title":"The evolution of antibiotic resistance is associated with collateral drug phenotypes in Mycobacterium tuberculosis","volume":"14","author":"Waller","year":"2023","journal-title":"Nat Commun"},{"issue":"6","key":"10.1016\/j.ebiom.2025.105776_bib52","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.02795-22","article-title":"Origin and dynamics of Mycobacterium tuberculosis subpopulations that predictably generate drug tolerance and resistance","volume":"13","author":"Sebastian","year":"2022","journal-title":"mBio"}],"container-title":["eBioMedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396425002208?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352396425002208?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T21:14:13Z","timestamp":1752614053000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352396425002208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":51,"alternative-id":["S2352396425002208"],"URL":"https:\/\/doi.org\/10.1016\/j.ebiom.2025.105776","relation":{},"ISSN":["2352-3964"],"issn-type":[{"value":"2352-3964","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Diverse impacts of different rpoB mutations on the anti-tuberculosis efficacy of capreomycin","name":"articletitle","label":"Article Title"},{"value":"eBioMedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ebiom.2025.105776","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"105776"}}