{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T02:18:32Z","timestamp":1793153912440,"version":"4.5.0"},"reference-count":109,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100006755","name":"School of Life Sciences, Arizona State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006755","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004853","name":"Chinese University of Hong Kong","doi-asserted-by":"publisher","award":["2016-18"],"award-info":[{"award-number":["2016-18"]}],"id":[{"id":"10.13039\/501100004853","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ecotoxicology and Environmental Safety"],"published-print":{"date-parts":[[2019,10]]},"DOI":"10.1016\/j.ecoenv.2019.109442","type":"journal-article","created":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T19:14:59Z","timestamp":1564082099000},"page":"109442","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":268,"special_numbering":"C","title":["Acute toxic effects of polyethylene microplastic on adult zebrafish"],"prefix":"10.1016","volume":"182","author":[{"given":"Chu Wa","family":"Mak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Ching-Fong Yeung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"King Ming","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ecoenv.2019.109442_bib1","series-title":"Mulit-residue Analysis of PAHs, PCBs and OCPs on an Agilent J&W FactorFour VF-5ms GC Column Application Note","author":"Agilent Technology","year":"2010"},{"key":"10.1016\/j.ecoenv.2019.109442_bib2","series-title":"Polycyclic Aromatic Hydrocarbon (PAH) Analysis in Fish byGC\/MS Using Agilent Bond Elut QuEChERS dSPE Sample Preparation and a High Efficiency DB-5ms Ultra Inert GC Column Application Note","author":"Agilent Technology","year":"2010"},{"key":"10.1016\/j.ecoenv.2019.109442_bib3","series-title":"Agilent QuEChERS Selection Guide","author":"Agilent Technology","year":"2011"},{"key":"10.1016\/j.ecoenv.2019.109442_bib5","first-page":"769","article-title":"Journal of AOAC International, 86(2)","volume":"386","author":"Anastassiades","year":"2003","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.ecoenv.2019.109442_bib6","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1016\/j.marpolbul.2011.05.030","article-title":"Microplastics in the marine environment","volume":"62","author":"Andrady","year":"2011","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2019.109442_bib100","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1476-5926-2-4","article-title":"Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption","volume":"2","author":"Arukwe","year":"2003","journal-title":"Comp. Hepatol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib7","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1196\/annals.1300.022","article-title":"Dopamine-acetylcholine interactions in the modulation of glutamate release","volume":"1003","author":"Atzori","year":"2003","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.ecoenv.2019.109442_bib8","doi-asserted-by":"crossref","first-page":"459","DOI":"10.3233\/JAD-2011-110704","article-title":"Evaluation of color preference in zebrafish for learning and memory","volume":"28","author":"Avdesh","year":"2011","journal-title":"J. Alzheimer's Dis."},{"key":"10.1016\/j.ecoenv.2019.109442_bib9","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.envpol.2013.10.007","article-title":"Enhanced desorption of persistent organic pollutants from microplastics under simulated physiological conditions","volume":"185","author":"Bakir","year":"2014","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.ecoenv.2019.109442_bib10","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.ecss.2014.01.004","article-title":"Transport of persistent organic pollutants by microplastics in estuarine conditions","volume":"140","author":"Bakir","year":"2014","journal-title":"Estuar. Coast Shelf Sci."},{"key":"10.1016\/j.ecoenv.2019.109442_bib101","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.aquatox.2017.12.008","article-title":"Microplastics cause neurotoxicity, oxidative damage and energy-related changes and interact with the bioaccumulation of mercury in the European seabass, Dicentrarchus labrax (Linnaeus, 1758)","volume":"195","author":"Barboza","year":"2018","journal-title":"Aqua. Toxicol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib11","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.chemosphere.2015.03.010","article-title":"Toxicity of leachate from weathering plastics: An exploratory screening study with Nitocra spinipes","volume":"132","author":"Bejgarn","year":"2015","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2019.109442_bib12","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/j.1528-1167.2010.02522.x","article-title":"Revised terminology and concepts for organization of seizures and epilepsies: Report of the ILAE Commission on Classification and Terminology, 2005-2009","volume":"51","author":"Berg","year":"2010","journal-title":"Epilepsia"},{"key":"10.1016\/j.ecoenv.2019.109442_bib13","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1021\/es302763x","article-title":"Effects of microplastic on fitness and PCB bioaccumulation by the lugworm Arenicola marina (L.)","volume":"47","author":"Besseling","year":"2013","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib14","doi-asserted-by":"crossref","first-page":"567","DOI":"10.2147\/NDT.S2056","article-title":"Zebrafish: An in vivo model for the study of neurological diseases","volume":"4","author":"Best","year":"2008","journal-title":"Neuropsychiatric Dis. Treat."},{"key":"10.1016\/j.ecoenv.2019.109442_bib15","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.bbr.2009.11.009","article-title":"Behavioral measures of anxiety in zebrafish (Danio rerio)","volume":"208","author":"Blaser","year":"2010","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.ecoenv.2019.109442_bib16","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1016\/j.marpolbul.2010.08.007","article-title":"Plastic ingestion by planktivorous fishes in the north pacific central gyre","volume":"60","author":"Boerger","year":"2010","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2019.109442_bib17","doi-asserted-by":"crossref","first-page":"5026","DOI":"10.1021\/es800249a","article-title":"Ingested microscopic plastic translocates to the circulatory system of the mussel, Mytilus edulis (L)","volume":"42","author":"Browne","year":"2008","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib18","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1016\/S0025-326X(01)00147-3","article-title":"Marine debris and human impacts on sea turtles in southern Brazil","volume":"42","author":"Bugoni","year":"2001","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2019.109442_bib105","doi-asserted-by":"crossref","first-page":"e17597","DOI":"10.1371\/journal.pone.0017597","article-title":"Three-dimensional neurophenotyping of adult zebrafish behavior","volume":"6","author":"Cachat","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.ecoenv.2019.109442_bib19","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.bbr.2010.06.001","article-title":"Translating rodent behavioral repertoire to zebrafish (Danio rerio): Relevance for stress research","volume":"214","author":"Champagne","year":"2010","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.ecoenv.2019.109442_bib20","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.3390\/ijerph110303156","article-title":"Toxicity and estrogenic endocrine disrupting activity of phthalates and their mixtures","volume":"11","author":"Chen","year":"2014","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.ecoenv.2019.109442_bib21","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s00418-009-0619-8","article-title":"Complementary developmental expression of the two tyrosine hydroxylase transcripts in zebrafish","volume":"132","author":"Chen","year":"2009","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib22","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.chemosphere.2015.12.083","article-title":"Regulation of the expression of estrogen receptor pathway related genes by TCDD, Cd2+ and mixtures of Cd2+ and TCDD in zebrafish","volume":"147","author":"Chen","year":"2016","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2019.109442_bib23","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.chemosphere.2018.07.032","article-title":"Modulations of TCDD-mediated induction of zebrafish cytochrome P450 1A1 by heavy metal ions in zebrafish","volume":"210","author":"Chen","year":"2018","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2019.109442_bib24","doi-asserted-by":"crossref","first-page":"597","DOI":"10.3390\/ijerph15040597","article-title":"Microplastic contamination of wild and captive flathead grey mullet (Mugil cephalus)","volume":"15","author":"Cheung","year":"2018","journal-title":"Environ. Res. Public Health"},{"key":"10.1016\/j.ecoenv.2019.109442_bib26","doi-asserted-by":"crossref","first-page":"2588","DOI":"10.1016\/j.marpolbul.2011.09.025","article-title":"Microplastics as contaminants in the marine environment: A review","volume":"62","author":"Cole","year":"2011","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2019.109442_bib27","doi-asserted-by":"crossref","first-page":"315","DOI":"10.2174\/1570159X11311030006","article-title":"Acetylcholinesterase inhibitors: pharmacology and toxicology","volume":"11","author":"Colovic","year":"2013","journal-title":"Curr. Neuropharmacol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib28","series-title":"Materials Safety Data Sheet of PE Microplastic","author":"Cospheric","year":"2017"},{"key":"10.1016\/j.ecoenv.2019.109442_bib111","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1124\/dmd.115.064485","article-title":"Activity Suppression Behavior Phenotype in SULT4A1 Frameshift Mutant Zebrafish","volume":"43","author":"Crittenden","year":"2015","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.ecoenv.2019.109442_bib29","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1007\/s11356-011-0579-0","article-title":"The seasonal and spatial patterns of ingestion of polyfilament nylon fragments by estuarine drums (Sciaenidae)","volume":"19","author":"Dantas","year":"2012","journal-title":"Environ. Sci. Pollut. Control Ser."},{"key":"10.1016\/j.ecoenv.2019.109442_bib30","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.bbi.2006.09.006","article-title":"Twenty years of research on cytokine-induced sickness behavior","volume":"21","author":"Dantzer","year":"2007","journal-title":"Brain Behav. Immun."},{"key":"10.1016\/j.ecoenv.2019.109442_bib31","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.envpol.2014.10.026","article-title":"Effects of microplastics on juveniles of the common goby Pomatoschistus microps: Confusion with prey, reduction of the predatory performance and efficiency, and possible influence of developmental conditions","author":"de Sa","year":"2015","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.ecoenv.2019.109442_bib32","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.marpolbul.2013.12.035","article-title":"Widespread distribution of microplastics in subsurface seawater in the NE Pacific Ocean","volume":"79","author":"Desforges","year":"2014","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2019.109442_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12302-015-0069-y","article-title":"Microplastics in the aquatic and terrestrial environment: Sources (with a specific focus on personal care products), fate and effects","volume":"28","author":"Duis","year":"2016","journal-title":"Environ. Sci. Eur."},{"key":"10.1016\/j.ecoenv.2019.109442_bib34","first-page":"4501","article-title":"Presence of microplastics and nanoplastics in food, with particular focus on seafood","volume":"14","year":"2016","journal-title":"Eur. Food Saf. Auth."},{"key":"10.1016\/j.ecoenv.2019.109442_bib35","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.bbr.2009.06.022","article-title":"Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish","volume":"205","author":"Egan","year":"2009","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.ecoenv.2019.109442_bib36","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.ecoenv.2006.06.003","article-title":"Hematological and gill histopathological parameters of three tropical fish species from a polluted lagoon on the west coast of Mauritius","volume":"68","author":"Elahee","year":"2007","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2019.109442_bib37","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jsbmb.2009.07.001","article-title":"Characterization of vtg-1 mRNA expression during ontogeny in Oreochromis mossambicus (PETERS)","volume":"117","author":"Esterhuyse","year":"2009","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib38","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.chemosphere.2014.08.047","article-title":"The partition behavior of perfluorooctanesulfonate (PFOS) and perfluorooctanesulfonamide (FOSA) on microplastics","volume":"119","author":"Fei","year":"2015","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2019.109442_bib39","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.cbpc.2005.03.002","article-title":"Annual changes in serum levels of two choriogenins and vitellogenin in masu salmon, Oncorhynchus masou","volume":"141","author":"Fujita","year":"2005","journal-title":"Comp. Biochem. Physiol., B"},{"key":"10.1016\/j.ecoenv.2019.109442_bib40","doi-asserted-by":"crossref","first-page":"11376","DOI":"10.1523\/JNEUROSCI.2311-11.2011","article-title":"Inflammation-induced lethargy is mediated by suppression of orexin neuron activity","volume":"31","author":"Grossberg","year":"2011","journal-title":"J. Neurosci."},{"key":"10.1016\/j.ecoenv.2019.109442_bib41","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1080\/19440049.2011.555845","article-title":"Migration of plasticizersphthalates, bisphenol A and alkylphenols from plastic containers and evaluation of risk","volume":"28","author":"Guart","year":"2011","journal-title":"Food Addit. Contam."},{"key":"10.1016\/j.ecoenv.2019.109442_bib42","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.bbr.2011.12.027","article-title":"Lipopolysaccharide affects exploratory behaviors toward novel objects by impairing cognition and\/or motivation in mice: possible role of activation of the central amygdala","volume":"228","author":"Haba","year":"2012","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.ecoenv.2019.109442_bib43","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.bbi.2013.10.021","article-title":"Functions beyond times of pathogen exposure?","volume":"2943","author":"Hennesey","year":"2014","journal-title":"Brain Behav. Immun."},{"key":"10.1016\/j.ecoenv.2019.109442_bib44","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s00227-005-0214-z","article-title":"Multiple piscine vitellogenins: Biomarkers of fish exposure to estrogenic endocrine disruptors in aquatic environments","volume":"149","author":"Hiramatsu","year":"2006","journal-title":"Mar. Biol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib45","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.chemosphere.2016.09.002","article-title":"Uptake, accumulation and elimination of polystyrene microspheres in tadpoles of Xenopus tropicals","volume":"164","author":"Hu","year":"2016","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2019.109442_bib46","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.watres.2016.03.015","article-title":"Pigments and plastic in limnetic ecosystems: A qualitative and quantitative study on microparticles of different size classes","volume":"98","author":"Imhof","year":"2016","journal-title":"Water Res."},{"key":"10.1016\/j.ecoenv.2019.109442_bib47","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/dneu.20873","article-title":"Fear, anxiety and control in the zebrafish","volume":"72","author":"Jesuthasan","year":"2012","journal-title":"Dev. Neurobiol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib48","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1021\/jf0345696","article-title":"Migration of nonylphenol from plastic containers to water and a milk surrogate","volume":"52","author":"Jorge","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.ecoenv.2019.109442_bib49","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1002\/ieam.1913","article-title":"Ingestion of microplastic by fish and its potential consequences from a physical perspective","volume":"13","author":"Jovanovi\u0107","year":"2017","journal-title":"Integr. Environ. Assess. Manag."},{"key":"10.1016\/j.ecoenv.2019.109442_bib50","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1089\/zeb.2012.0861","article-title":"Towards a comprehensive catalog of zebrafish behavior 1.0 and beyond","volume":"10","author":"Kalueff","year":"2013","journal-title":"Zebrafish"},{"key":"10.1016\/j.ecoenv.2019.109442_bib51","series-title":"The Rights and Wrongs of Zebrafish: Behavioral Phenotyping of Zebrafish","author":"Kalueff","year":"2017"},{"key":"10.1016\/j.ecoenv.2019.109442_bib52","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.envpol.2017.01.047","article-title":"Biomarker responses in zebrafish (Danio rerio) larvae exposed to pristine low-density polyethylene fragments","volume":"223","author":"Karami","year":"2017","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.ecoenv.2019.109442_bib53","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.envres.2016.07.024","article-title":"Virgin microplastics cause toxicity and modulate the impacts of phenanthrene on biomarker responses in African catfish (Clarias gariepinus)","volume":"151","author":"Karami","year":"2016","journal-title":"Environ. Res."},{"key":"10.1016\/j.ecoenv.2019.109442_bib54","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S0889-1591(02)00077-6","article-title":"Cytokine-induced sickness behavior","volume":"17","author":"Kelley","year":"2003","journal-title":"Brain Behav. Immun."},{"key":"10.1016\/j.ecoenv.2019.109442_bib55","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.scitotenv.2018.08.310","article-title":"Zebrafish can recognize microplastics as inedible materials: Quantitative evidence of ingestion behavior","volume":"649","author":"Kim","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2019.109442_bib56","series-title":"Marine Debris. Springer Series on Environmental Management","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/978-1-4613-8486-1_10","article-title":"Impacts of marine debris: Entanglement of marine life in marine debris including a comprehensive list of species with entanglement and ingestion records","author":"Laist","year":"1997"},{"key":"10.1016\/j.ecoenv.2019.109442_bib57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aquaculture.2007.04.077","article-title":"The husbandry of zebrafish (Danio rerio): A review","volume":"269","author":"Lawrence","year":"2017","journal-title":"Aquaculture"},{"key":"10.1016\/j.ecoenv.2019.109442_bib58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.scitotenv.2017.11.103","article-title":"Microplastic particles cause intestinal damage and other adverse effects in zebrafish Danio rerio and nematode Caenorhabditis elegans","volume":"619\u2013620","author":"Lei","year":"2018","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2019.109442_bib59","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1073\/pnas.1606615113","article-title":"Microplastic exposure studies should be environmentally realistic","volume":"113","author":"Lenz","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"10.1016\/j.ecoenv.2019.109442_bib60","doi-asserted-by":"crossref","first-page":"3309","DOI":"10.1016\/j.scitotenv.2011.04.038","article-title":"Environmental and health hazard ranking and assessment of plastic polymers based on chemical composition","volume":"409","author":"Lithner","year":"2011","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2019.109442_bib108","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s00359-011-0682-1","article-title":"Alternative startle motor pat- terns and behaviors in the larval zebrafish (Danio rerio)","volume":"198","author":"Liu","year":"2012","journal-title":"J. Comp. Physiol. A. Neuroethol. Sens. Neural. Behav. Physiol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib61","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.ecoenv.2019.109442_bib62","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.marpolbul.2012.11.028","article-title":"Occurrence of microplastics in the gastrointestinal tract of pelagic and demersal fish from the English Channel","volume":"67","author":"Lusher","year":"2013","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2019.109442_bib63","doi-asserted-by":"crossref","first-page":"4054","DOI":"10.1021\/acs.est.6b00183","article-title":"Uptake and accumulation of polystyrene microplastics in zebrafish (Danio rerio) and toxic effects in liver","volume":"50","author":"Lu","year":"2016","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib64","series-title":"Microplastic and Microbead Contamination Entering into Victoria Harbor, Hong Kong","author":"Mak","year":"2019"},{"key":"10.1016\/j.ecoenv.2019.109442_bib65","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1021\/es5053655","article-title":"Altered behavior, physiology, and metabolism in fish exposed to polystyrene nanoparticles","volume":"49","author":"Mattsson","year":"2015","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib104","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.bbr.2010.05.031","article-title":"Measuring anxiety in zebrafish: A critical review","volume":"214","author":"Maximino","year":"2010","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.ecoenv.2019.109442_bib66","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.marenvres.2015.09.009","article-title":"Evaluation of the impact of polyethylene microbeads ingestion in European sea bass (Dicentrarchus labrax) larvae","volume":"112","author":"Mazurais","year":"2015","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.ecoenv.2019.109442_bib67","doi-asserted-by":"crossref","first-page":"13379","DOI":"10.1039\/c3ta12555f","article-title":"Progress in bio-based plastics and plasticizing modifications","volume":"1","author":"Mekonnen","year":"2013","journal-title":"J. Mater. Chem."},{"key":"10.1016\/j.ecoenv.2019.109442_bib68","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1897\/05-683R.1","article-title":"Vitellogenin 1 mRNA as an early molecular biomarker for endocrine disruption in developing zebrafish (Danio rerio)","volume":"25","author":"Muncke","year":"2006","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.ecoenv.2019.109442_bib69","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1023\/A:1007702106948","article-title":"Intrahepatic expression of genes encoding choriogenins: precursor proteins of the egg envelope of fish, the medaka, Oryzias latipes","volume":"17","author":"Murata","year":"1997","journal-title":"Fish Physiol. Biochem."},{"key":"10.1016\/j.ecoenv.2019.109442_bib70","first-page":"107","article-title":"Additives for Plastics Handbook. 2nd ed. Oxford (UK)","volume":"1\u20134","author":"Murphy","year":"2001","journal-title":"Elsevier Science"},{"key":"10.1016\/j.ecoenv.2019.109442_bib71","doi-asserted-by":"crossref","first-page":"2377","DOI":"10.1021\/ma902740t","article-title":"Phthalate plasticizers covalently bound to PVC: plasticization with suppressed migration","volume":"43","author":"Navarro","year":"2010","journal-title":"Macromolecules"},{"key":"10.1016\/j.ecoenv.2019.109442_bib72","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1098\/rstb.2008.0242","article-title":"A critical analysis of the biological impacts of plasticizers on wildlife","volume":"364","author":"Oehlmann","year":"2009","journal-title":"Phil. Trans. Roy. Soc."},{"key":"10.1016\/j.ecoenv.2019.109442_bib106","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1089\/zeb.2011.0690","article-title":"Fighting zebrafish: Characterization of aggressive behavior and winner-loser effects","volume":"8","author":"Oliveira","year":"2011","journal-title":"Zebrafish"},{"key":"10.1016\/j.ecoenv.2019.109442_bib73","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S0301-0082(97)00085-3","article-title":"Glutamate receptors in the mammalian central nervous system","volume":"54","author":"Ozawa","year":"1998","journal-title":"Prog. Neurobiol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib74","series-title":"Practical Guide to Polyvinyl Chloride","author":"Patrick","year":"2005"},{"key":"10.1016\/j.ecoenv.2019.109442_bib75","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1016\/j.bcp.2007.01.014","article-title":"Identification of zebrafish histamine H1, H2 and H3 receptors and effects of histaminergic ligands on behavior","volume":"73","author":"Peitsaro","year":"2007","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib76","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1016\/j.marpolbul.2011.01.036","article-title":"Plastic debris ingestion by marine catfish: An unexpected fisheries impact","volume":"62","author":"Possatto","year":"2011","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2019.109442_bib77","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1002\/tox.20484","article-title":"Sulfur dioxide and benzo(a)pyrene modulates CYP1A and tumor-related gene expression in rat liver","volume":"25","author":"Qin","year":"2010","journal-title":"Environ. Toxicol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib78","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1021\/es103934x","article-title":"Species-specific differences and structure- activity relationships in the debromination of PBDE congeners in three fish species","volume":"45","author":"Roberts","year":"2011","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib79","first-page":"1646","article-title":"Long-term field measurement of sorption of organic contaminants to five types of plastic pellets: Implications for plastic marine debris","volume":"47","author":"Rochman","year":"2013","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib80","doi-asserted-by":"crossref","first-page":"3263","DOI":"10.1038\/srep03263","article-title":"Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress","volume":"3","author":"Rochman","year":"2013","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ecoenv.2019.109442_bib102","doi-asserted-by":"crossref","first-page":"e0187664","DOI":"10.1371\/journal.pone.0187664","article-title":"Direct and indirect effects of different types of microplastics on freshwater prey (Corbicula fulminea) and their predator (Acipenser transmontanus)","volume":"12","author":"Rochman","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.ecoenv.2019.109442_bib81","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.1897\/08-559.1","article-title":"A comparison of nanoparticle and fine particle uptake by Daphnia magna","volume":"28","author":"Rosenkranz","year":"2009","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.ecoenv.2019.109442_bib82","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1002\/ieam.1681","article-title":"Out of sight, but no longer out of mind: Microplastics as a global pollutant","volume":"11","author":"Ross","year":"2015","journal-title":"Integr. Environ. Assess. Manag."},{"key":"10.1016\/j.ecoenv.2019.109442_bib109","doi-asserted-by":"crossref","first-page":"43","DOI":"10.46867\/IJCP.2010.23.01.06","article-title":"Zebrafish behavior in novel environments: Effects of acute exposure to anxiolytic compounds and choice of Danio rerio Line","volume":"23","author":"Sackerman","year":"2010","journal-title":"Int. J. Comp. Psychol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib83","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1002\/ieam.1909","article-title":"Microplastic as contaminants in commercially important seafood species","volume":"13","author":"Santillo","year":"2017","journal-title":"Integr. Environ. Assess. Manag."},{"key":"10.1016\/j.ecoenv.2019.109442_bib84","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.aca.2012.10.034","article-title":"Multi-class, multi-residue analysis of pesticides, polychlorinated biphenyls, polycyclic aromatic hydrocarbons, polybrominated diphenyl ethers and novel flame retardants in fish using fast, low pressure gas chromatography-tandem mass spectrometry","volume":"758","author":"Sapozhnikova","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.ecoenv.2019.109442_bib85","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0048-9697(99)00500-8","article-title":"Cytochrome P4501A (CYP1A) in teleostean fishes. A review of immunohistochemical studies","volume":"247","author":"Sarasquete","year":"2000","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2019.109442_bib107","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/978-1-60761-953-6_10","article-title":"Measuring agonistic behavior in zebrafish. Zebrafish Neurobehavior Protocol","volume":"51","author":"Schneider","year":"2011","journal-title":"Neuromethods"},{"key":"10.1016\/j.ecoenv.2019.109442_bib86","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1242\/dev.02827","article-title":"Zebrafish curly up encodes a Pkd2 ortholog that restricts left-side-specific expression of southpaw","volume":"134","author":"Schottenfeld","year":"2007","journal-title":"Development"},{"key":"10.1016\/j.ecoenv.2019.109442_bib87","doi-asserted-by":"crossref","first-page":"380","DOI":"10.4103\/0250-474X.117432","article-title":"Effect of Eclipta Alba on acute seizure models: A GABA-mediated effect","volume":"75","author":"Shaikh","year":"2013","journal-title":"Indian J. Pharm. Sci."},{"key":"10.1016\/j.ecoenv.2019.109442_bib88","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1002\/neu.10169","article-title":"Molecular and cellular cognitive studies of the role of synaptic plasticity in memory","volume":"54","author":"Silva","year":"2003","journal-title":"J. Neurobiol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib103","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.bbr.2007.10.031","article-title":"Alarm substance induced behavioral responses in zebrafish (Danio rerio)","volume":"188","author":"Speedie","year":"2008","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.ecoenv.2019.109442_bib89","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1515\/rns.2011.011","article-title":"Zebrafish models to study drug abuse\u2013related phenotypes","volume":"22","author":"Stweart","year":"2010","journal-title":"Rev. Neurosci."},{"key":"10.1016\/j.ecoenv.2019.109442_bib90","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.marpolbul.2016.11.003","article-title":"Microplastic pollution in the marine waters and sediments of Hong Kong","volume":"115","author":"Tsang","year":"2017","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2019.109442_bib91","doi-asserted-by":"crossref","first-page":"11327","DOI":"10.1021\/es302332w","article-title":"Uptake and effects of microplastics on cells and tissue of the blue mussel Mytilus edulis L. after an experimental exposure","volume":"46","author":"Von","year":"2012","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib92","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.gene.2005.03.041","article-title":"Hepatic and extrahepatic expression of vitellogenin genes in the zebrafish, Danio rerio","volume":"356","author":"Wang","year":"2005","journal-title":"Gene"},{"key":"10.1016\/j.ecoenv.2019.109442_bib93","doi-asserted-by":"crossref","first-page":"8823","DOI":"10.1021\/es501090e","article-title":"Uptake and retention of microplastics by the shore crab Carcinus maenas","volume":"48","author":"Watts","year":"2014","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2019.109442_bib94","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1016\/j.marpolbul.2006.06.009","article-title":"Trace organic contamination in biota collected from the Pearl River Estuary, China: A preliminary risk assessment","volume":"52","author":"Wei","year":"2006","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2019.109442_bib95","doi-asserted-by":"crossref","first-page":"4933","DOI":"10.2174\/092986711797535191","article-title":"Classical neurotransmitters and neuropeptides involved in generalized epilepsy: A focus on antiepileptic drugs","volume":"18","author":"Werner","year":"2011","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.ecoenv.2019.109442_bib110","series-title":"The zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Danio Rerio)","volume":"1","author":"Westerfield","year":"2007"},{"key":"10.1016\/j.ecoenv.2019.109442_bib96","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.envpol.2013.02.031","article-title":"The physical impacts of microplastics on marine organisms: A review","volume":"178","author":"Wright","year":"2013","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.ecoenv.2019.109442_bib97","series-title":"Handbook of Plasticizers","first-page":"7","article-title":"Plasticizer types","author":"Wypych","year":"2017"},{"key":"10.1016\/j.ecoenv.2019.109442_bib98","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1289\/ehp.1003220","article-title":"Most plastic products release estrogenic chemicals: A potential health problem that can be solved","volume":"119","author":"Yang","year":"2011","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.ecoenv.2019.109442_bib99","doi-asserted-by":"crossref","first-page":"3794","DOI":"10.1021\/acs.est.7b00369","article-title":"Occurrence and characteristics of microplastic pollution in Xiangxi Bay of three gorges Reservoir, China","volume":"51","author":"Zhang","year":"2017","journal-title":"Environ. Sci. Technol."}],"container-title":["Ecotoxicology and Environmental Safety"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651319307730?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651319307730?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T18:57:37Z","timestamp":1777489057000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147651319307730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":109,"alternative-id":["S0147651319307730"],"URL":"https:\/\/doi.org\/10.1016\/j.ecoenv.2019.109442","relation":{},"ISSN":["0147-6513"],"issn-type":[{"value":"0147-6513","type":"print"}],"subject":[],"published":{"date-parts":[[2019,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Acute toxic effects of polyethylene microplastic on adult zebrafish","name":"articletitle","label":"Article Title"},{"value":"Ecotoxicology and Environmental Safety","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ecoenv.2019.109442","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109442"}}