{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:33:03Z","timestamp":1792456383025,"version":"4.5.0"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T00:00:00Z","timestamp":1605916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2013 1348"],"award-info":[{"award-number":["PICT 2013 1348"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007070","name":"Universidad Nacional de Mar del Plata","doi-asserted-by":"publisher","award":["EXA 795\/16"],"award-info":[{"award-number":["EXA 795\/16"]}],"id":[{"id":"10.13039\/501100007070","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007070","name":"Universidad Nacional de Mar del Plata","doi-asserted-by":"publisher","award":["EXA899\/18"],"award-info":[{"award-number":["EXA899\/18"]}],"id":[{"id":"10.13039\/501100007070","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ecotoxicology and Environmental Safety"],"published-print":{"date-parts":[[2020,12]]},"DOI":"10.1016\/j.ecoenv.2020.111157","type":"journal-article","created":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T12:53:27Z","timestamp":1597928007000},"page":"111157","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["Sublethal effects of carbendazim in Jenynsia multidentata detected by a battery of molecular, biochemical and genetic biomarkers"],"prefix":"10.1016","volume":"205","author":[{"given":"Jesica Y.","family":"G\u00f6tte","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6175-6245","authenticated-orcid":false,"given":"Juan Cruz","family":"Carrizo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3935-8758","authenticated-orcid":false,"given":"Ana M.","family":"Panzeri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Valeria","family":"Am\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirta L.","family":"Menone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ecoenv.2020.111157_bib1","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1080\/03601230902997766","article-title":"An evaluation of the cytochrome P450 inhibition potential of selected pesticides in human hepatic microsomes","volume":"44","author":"Abass","year":"2009","journal-title":"Environ. Sc. Health Part B"},{"key":"10.1016\/j.ecoenv.2020.111157_bib2","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1016\/j.chemosphere.2008.11.068","article-title":"Effects of microcystin\u2013LR on the expression of P-glycoprotein in Jenynsia multidentata","volume":"74","author":"Am\u00e9","year":"2009","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2020.111157_bib3","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.aquatox.2015.11.017","article-title":"Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos","volume":"170","author":"Andrade","year":"2016","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib4","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1046\/j.1432-1327.1999.00225.x","article-title":"Structural and mechanistic aspects of transcriptional induction of cytochrome P450 1A1 by benzimidazole derivatives in rat hepatoma H4IIE cells","volume":"261","author":"Backlund","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.ecoenv.2020.111157_bib5","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1080\/03601230701309577","article-title":"Endosulfan acute toxicity and histomorphological alterations in Jenynsia multidentata (Anablepidae, Cyprinodontiformes)","volume":"42","author":"Ballesteros","year":"2007","journal-title":"J. Environ. Sci. Health Part B Pestic. Food Contam. Agric. Wastes"},{"key":"10.1016\/j.ecoenv.2020.111157_bib6","doi-asserted-by":"crossref","first-page":"S93","DOI":"10.1016\/j.aquatox.2006.02.015","article-title":"Assessment of micronuclei induction in peripheral erythrocytes of fish exposed to xenobiotics under controlled conditions","volume":"78","author":"Bolognesi","year":"2006","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib7","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1002\/etc.3613","article-title":"Tissue-specific bioconcentration and biotransformation of cypermethrin and chlorpyrifos in a native fish (Jenynsia multidentata) exposed to these insecticides singly and in mixture","volume":"36","author":"Bonansea","year":"2017","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.ecoenv.2020.111157_bib8","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein- dyebinding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ecoenv.2020.111157_bib9","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1139\/f90-237","article-title":"Assessment of the piscine micronucleus test as an in situ biological indicator of chemical contaminant effects","volume":"47","author":"Carrasco","year":"1990","journal-title":"Can. J. Fish. Aquat. Sci."},{"key":"10.1016\/j.ecoenv.2020.111157_bib10","series-title":"Gu\u00eda de Productos Fitosanitarios 2017\/2019","author":"CASAFE","year":"2017"},{"key":"10.1016\/j.ecoenv.2020.111157_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aquatox.2005.08.011","article-title":"Differential detoxification and antioxidant response in diverse organs of Corydoras paleatus experimentally exposed to microcystin-RR","volume":"76","author":"Cazenave","year":"2006","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib12","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ecoenv.2020.111157_bib13","series-title":"CRC Handbook of Methods in Oygen Radical Research","first-page":"283","article-title":"Catalase activity","author":"Claiborne","year":"1985"},{"key":"10.1016\/j.ecoenv.2020.111157_bib14","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00128-019-02758-z","article-title":"Pesticides in surface waters in Argentina monitored using polar organic chemical integrative samplers","volume":"104","author":"Corcoran","year":"2020","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib16","author":"EC"},{"key":"10.1016\/j.ecoenv.2020.111157_bib17","first-page":"4791","article-title":"The 2015 European Union report on pesticide residues in food. European Food Safety Authority","volume":"15","year":"2017","journal-title":"EFSA Journal"},{"key":"10.1016\/j.ecoenv.2020.111157_bib18","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid colorimetric determination of acetylcholinesterase activity","volume":"7","author":"Ellman","year":"1961","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib19","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1002\/bdrb.20290","article-title":"Developmental toxicity of fungicide carbendazim in female mice","volume":"92","author":"Farag","year":"2011","journal-title":"Birth Def. Res. (Part B)"},{"key":"10.1016\/j.ecoenv.2020.111157_bib20","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1093\/mutage\/geq052","article-title":"Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells","volume":"26","author":"Fenech","year":"2011","journal-title":"Mutagenesis"},{"key":"10.1016\/j.ecoenv.2020.111157_bib21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mrrev.2018.11.001","article-title":"The molecular origins and pathophysiological consequences of micronuclei: new insights into an age-old problem","volume":"779","author":"Guo","year":"2019","journal-title":"Mutat. Res."},{"key":"10.1016\/j.ecoenv.2020.111157_bib22","first-page":"1730","article-title":"Glutathione S- transferase, the first step in mercapturic acid formation","volume":"249","author":"Habig","year":"1974","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ecoenv.2020.111157_bib23","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1002\/etc.3574","article-title":"Uptake, distribution in different tissues and genotoxicity of imidacloprid in the freshwater fish Australoheros facetus","volume":"36","author":"Iturburu","year":"2017","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.ecoenv.2020.111157_bib24","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.scitotenv.2019.07.044","article-title":"Ecological Risk Assessment (ERA) of pesticides from freshwater ecosystems in Pampas region of Argentina: legacy and current use chemicals contribution","volume":"691","author":"Iturburu","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2020.111157_bib25","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.fsi.2014.09.037","article-title":"Embryonic exposure to carbendazim induces the transcription of genes related to apoptosis, immunotoxicity and endocrine disruption in zebrafish (Danio rerio)","volume":"41","author":"Jiang","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib26","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1016\/j.tiv.2015.06.003","article-title":"Carbendazim has the potential to induce oxidative stress, apoptosis, immunotoxicity and endocrine disruption during zebrafish larvae development","volume":"29","author":"Jiang","year":"2015","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.ecoenv.2020.111157_bib27","series-title":"Proposed EQS for Water Framework Directive Annex VIII Substances: Carbendazim (For Consultation)","first-page":"60","author":"Johnson","year":"2012"},{"key":"10.1016\/j.ecoenv.2020.111157_bib28","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1016\/j.scitotenv.2017.12.225","article-title":"Biocides in the river system of a highly urbanized region: a systematic investigation involving runoff input","volume":"624","author":"Liu","year":"2018","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2020.111157_bib29","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.talanta.2012.08.006","article-title":"Determination of some organic contaminants in water samples by solid-phase extraction and liquid chromatography-tandem mass spectrometry","volume":"100","author":"Maldaner","year":"2012","journal-title":"Talanta"},{"key":"10.1016\/j.ecoenv.2020.111157_bib30","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.scitotenv.2014.06.095","article-title":"Pesticide monitoring in the basin of Llobregat River (Catalonia, Spain) and comparison with historical data","volume":"503\u2013504","author":"Masi\u00e1","year":"2015","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2020.111157_bib31","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.envpol.2018.04.040","article-title":"Occurrence and overlooked sources of the biocide carbendazim in wastewater and surface water","volume":"239","author":"Merel","year":"2018","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.ecoenv.2020.111157_bib32","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.pestbp.2008.07.007","article-title":"Effect of waterborne benomyl on the hematological and antioxidant parameters of the Nile tilapia","volume":"92","author":"Min","year":"2008","journal-title":"Oreochromis niloticus. Pest. Biochem. Physiol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib34","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0166-445X(02)00204-7","article-title":"Utility of TBARS assay in detecting oxidative stress in white sucker (Catostomus commersoni) populations exposed to pulp mill effluent","volume":"63","author":"Oakes","year":"2003","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib35","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1007\/s13762-013-0264-6","article-title":"Toxicity, biochemical and clastogenic response of chlorpyrifos and carbendazim in milkfish Chanos chanos","volume":"11","author":"Palanikumar","year":"2014","journal-title":"Int. J. Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib36","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.chemosphere.2004.08.047","article-title":"Pesticide levels in surface waters in an agricultural\u2013forestry basin in Southern Chile","volume":"57","author":"Palma","year":"2004","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2020.111157_bib37","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1080\/10807039.2012.743827","article-title":"Applying health risk analysis to assess the chemical quality of water for recreational bathing: case of Tres Arroyos Creek, Buenos Aires, Argentina","volume":"20","author":"Peluso","year":"2014","journal-title":"Hum. Ecol. Risk Assess."},{"key":"10.1016\/j.ecoenv.2020.111157_bib38","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ecoenv.2020.111157_bib39","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1002\/hep.23945","article-title":"Mmp23b promotes liver development and hepatocyte proliferation through the tumor necrosis factor pathway in zebrafish","volume":"52","author":"Qi","year":"2010","journal-title":"Hepatology"},{"key":"10.1016\/j.ecoenv.2020.111157_bib40","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1006\/bbrc.1996.0482","article-title":"Structural requirements for the induction of cytochromes P450 by benzimidazole anthelmintic derivatives in cultured rabbit hepatocytes","volume":"220","author":"Rey-Grobellet","year":"1996","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ecoenv.2020.111157_bib41","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.etap.2009.06.005","article-title":"The inducibility of human cytochrome P450 1A by environmental-relevant xenobiotics in the human hepatoma derived cell line HepG2","volume":"28","author":"Rudzok","year":"2009","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib42","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0027-5107(94)90018-3","article-title":"Evaluation of benomyl and carbendazim in the in vivo aneuploidy\/micronucleus assay in BDF1 mouse bone marrow","volume":"310","author":"Sarrif","year":"1994","journal-title":"Mutat. Res."},{"key":"10.1016\/j.ecoenv.2020.111157_bib43","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/s10311-016-0566-2","article-title":"Toxicity, monitoring and biodegradation of the fungicide carbendazim","volume":"14","author":"Singh","year":"2016","journal-title":"Environ. Chem. Lett."},{"key":"10.1016\/j.ecoenv.2020.111157_bib44","series-title":"Desarrollos de niveles gu\u00eda nacionales de calidad de agua ambiente correspondientes a carbendazim","author":"S","year":"2014"},{"key":"10.1016\/j.ecoenv.2020.111157_bib45","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.ecoenv.2005.03.013","article-title":"Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants","volume":"64","author":"Valavanidis","year":"2006","journal-title":"Ecotoxicol. Environ. Safe"},{"key":"10.1016\/j.ecoenv.2020.111157_bib46","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.taap.2016.06.004","article-title":"Activation of aryl hydrocarbon receptor reduces carbendazim-induced cell death","volume":"306","author":"Wei","year":"2016","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib47","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1021\/tx050321u","article-title":"Endogenous reactive intermediates as modulators of cell signaling and cell death","volume":"19","author":"West","year":"2006","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib48","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1002\/1522-2632(200008)85:4<413::AID-IROH413>3.0.CO;2-3","article-title":"Activity development of selected detoxication enzymes during the ontogenesis of the zebrafish (Danio rerio)","volume":"85","author":"Wiegand","year":"2000","journal-title":"Int. Rev. Hydrobiol."},{"key":"10.1016\/j.ecoenv.2020.111157_bib49","series-title":"Biostatistical Analysis","author":"Zar","year":"1999"},{"key":"10.1016\/j.ecoenv.2020.111157_bib50","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3390\/biology5020023","article-title":"The function of fish cytokines","volume":"5","author":"Zou","year":"2016","journal-title":"Biology"}],"container-title":["Ecotoxicology and Environmental Safety"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651320309969?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651320309969?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T05:17:56Z","timestamp":1758950276000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147651320309969"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":48,"alternative-id":["S0147651320309969"],"URL":"https:\/\/doi.org\/10.1016\/j.ecoenv.2020.111157","relation":{},"ISSN":["0147-6513"],"issn-type":[{"value":"0147-6513","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Sublethal effects of carbendazim in Jenynsia multidentata detected by a battery of molecular, biochemical and genetic biomarkers","name":"articletitle","label":"Article Title"},{"value":"Ecotoxicology and Environmental Safety","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ecoenv.2020.111157","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"111157"}}