{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T17:02:31Z","timestamp":1772816551903,"version":"3.50.1"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ecotoxicology and Environmental Safety"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1016\/j.ecoenv.2020.111777","type":"journal-article","created":{"date-parts":[[2020,12,19]],"date-time":"2020-12-19T00:08:04Z","timestamp":1608336484000},"page":"111777","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissues"],"prefix":"10.1016","volume":"209","author":[{"given":"P.R.","family":"Deepa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Nalini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Surianarayanan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Krishnakumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ecoenv.2020.111777_bib1","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/nmat2448","article-title":"Ionic-liquid materials for the electrochemical challenges of the future","volume":"8","author":"Armand","year":"2009","journal-title":"Nat. Mater."},{"key":"10.1016\/j.ecoenv.2020.111777_bib2","doi-asserted-by":"crossref","DOI":"10.4081\/ejh.2012.12","article-title":"Application of alternative fixatives to formalin in diagnostic pathology","volume":"56","author":"Benerini Gatta","year":"2012","journal-title":"Eur. J. Histochem."},{"key":"10.1016\/j.ecoenv.2020.111777_bib3","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.anndiagpath.2008.07.004","article-title":"Histology without formalin?","volume":"12","author":"Buesa","year":"2008","journal-title":"Ann. Diagn. Pathol."},{"key":"10.1016\/j.ecoenv.2020.111777_bib4","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.1002\/jcc.24042","article-title":"Enhanced stability of the model mini-protein in amino acid ionic liquids and their aqueous solutions","volume":"36","author":"Chevrot","year":"2015","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.ecoenv.2020.111777_bib5","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/S1470-2045(04)01562-1","article-title":"Advice on formaldehyde and glycol ethers","volume":"5","author":"Cogliano","year":"2004","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.ecoenv.2020.111777_bib6","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0027-5107(97)00314-X","article-title":"DNA damage caused by common cytological fixatives","volume":"401","author":"Douglas","year":"1998","journal-title":"Mutat. Res."},{"key":"10.1016\/j.ecoenv.2020.111777_bib7","article-title":"From green chemistry to nature: the versatile role of low transition temperature mixtures","volume":"120","author":"Durand","year":"2015","journal-title":"Biochimie"},{"key":"10.1016\/j.ecoenv.2020.111777_bib8","doi-asserted-by":"crossref","first-page":"7132","DOI":"10.1021\/acs.chemrev.6b00562","article-title":"Biological activity of ionic liquids and their application in pharmaceutics and medicine","volume":"117","author":"Egorova","year":"2017","journal-title":"Chem. Rev."},{"key":"10.1016\/j.ecoenv.2020.111777_bib9","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1179\/his.2001.24.3.201","article-title":"Advanced concepts in fixation: 1. Effects of fixation on immunohistochemistry, reversibility of fixation and recovery of proteins, nucleic acids, and other molecules from fixed and processed tissues 2. Developmental methods of fixation","volume":"24","author":"Eltoum","year":"2001","journal-title":"J. Histotechnol."},{"key":"10.1016\/j.ecoenv.2020.111777_bib10","series-title":"Histotechnology","author":"FL","year":"2007"},{"key":"10.1016\/j.ecoenv.2020.111777_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0014253","article-title":"Elevated pressure improves the extraction and identification of proteins recovered from formalin-fixed, paraffin-embedded tissue surrogates","volume":"5","author":"Fowler","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.ecoenv.2020.111777_bib12","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1002\/bit.20928","article-title":"Unexpected improvement in stability and utility of cytochrome c by solution in biocompatible ionic liquids","volume":"94","author":"Fujita","year":"2006","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ecoenv.2020.111777_bib13","doi-asserted-by":"crossref","first-page":"4804","DOI":"10.1039\/b508238b","article-title":"Protein solubilising and stabilising ionic liquids","author":"Fujita","year":"2005","journal-title":"Chem. Commun."},{"key":"10.1016\/j.ecoenv.2020.111777_bib14","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1369\/jhc.7A7177.2007","article-title":"Proteome analysis of microdissected formalin-fixed and paraffin-embedded tissue specimens","volume":"55","author":"Guo","year":"2007","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.ecoenv.2020.111777_bib15","first-page":"1","article-title":"Working group on the evaluation of carcinogenic risks to humans formaldehyde, 2-butoxyethanol and 1-tert butoxypropan-2-ol","volume":"88","author":"IARC","year":"2006","journal-title":"IARC Monogr. Eval. Carcinog. Risks Hum."},{"key":"10.1016\/j.ecoenv.2020.111777_bib16","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1177\/002215549804600314","article-title":"Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tissue sections","volume":"46","author":"Ikeda","year":"1998","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.ecoenv.2020.111777_bib17","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.ssi.2017.11.012","article-title":"Ionic liquids in biotechnology and beyond","volume":"314","author":"Claus","year":"2018","journal-title":"Solid State Ion."},{"key":"10.1016\/j.ecoenv.2020.111777_bib18","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1179\/his.1991.14.4.251","article-title":"Comparison of fixatives for histologic evaluation of the canine eye","volume":"14","author":"Saby","year":"1991","journal-title":"J. Histotechnol."},{"key":"10.1016\/j.ecoenv.2020.111777_bib19","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1017\/S1551929500057060","article-title":"Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: what they are and what they do","volume":"1","author":"Kiernan","year":"2000","journal-title":"Microsc. Today"},{"key":"10.1016\/j.ecoenv.2020.111777_bib20","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1177\/0300985812441031","article-title":"Weigners fixative-an alternative to formalin fixation for histology with improved preservation of nucleic acids","volume":"50","author":"Klopfleisch","year":"2013","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.ecoenv.2020.111777_bib21","series-title":"Microwaves for the Art of Microscopy","author":"Kok","year":"2003"},{"key":"10.1016\/j.ecoenv.2020.111777_bib22","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1007\/s00216-008-2445-6","article-title":"Ionic liquids in sample preparation","volume":"393","author":"Liu","year":"2009","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.ecoenv.2020.111777_bib23","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1078\/0065-1281-00707","article-title":"Ionic liquids in embalming and tissue preservation. Can traditional formalin-fixation be replaced safely","volume":"105","author":"Majewski","year":"2003","journal-title":"Acta Histochem"},{"key":"10.1016\/j.ecoenv.2020.111777_bib24","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1351\/pac200072071391","article-title":"Ionicliquids. Green solvents for the future","volume":"72","author":"Earle","year":"2000","journal-title":"Pure Appl. Chem."},{"key":"10.1016\/j.ecoenv.2020.111777_bib25","doi-asserted-by":"crossref","first-page":"13457","DOI":"10.1039\/C4CC05086J","article-title":"Biological stability and activity of siRNA in ionic liquids","volume":"50","author":"Mazid","year":"2014","journal-title":"Chem. Commun."},{"key":"10.1016\/j.ecoenv.2020.111777_bib26","doi-asserted-by":"crossref","first-page":"8089","DOI":"10.1039\/C5CC01877C","article-title":"Inhibited fragmentation of mAbs in buffered ionic liquids","volume":"51","author":"Mazid","year":"2015","journal-title":"Chem. Commun."},{"key":"10.1016\/j.ecoenv.2020.111777_bib27","doi-asserted-by":"crossref","first-page":"5254","DOI":"10.1021\/jp068446a","article-title":"Choline saccharinate and choline acesulfamate: ionic liquids with low toxicities","volume":"111","author":"Nockemann","year":"2007","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.ecoenv.2020.111777_bib28","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.acthis.2005.02.003","article-title":"Ionic liquids as an alternative to formalin in histopathological diagnosis","volume":"107","author":"Pernak","year":"2005","journal-title":"Acta Histochem."},{"key":"10.1016\/j.ecoenv.2020.111777_bib29","doi-asserted-by":"crossref","first-page":"6817","DOI":"10.1002\/chem.200700285","article-title":"Choline-derivative-based ionic liquids","volume":"13","author":"Pernak","year":"2007","journal-title":"Chemistry"},{"key":"10.1016\/j.ecoenv.2020.111777_bib30","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.ecoenv.2016.05.005","article-title":"Comparative in vitro study of cholinium-based ionic liquids and deep eutectic solvents toward fish cell line","volume":"131","author":"Radosevic","year":"2016","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2020.111777_bib31","doi-asserted-by":"crossref","first-page":"43839","DOI":"10.1039\/C5RA05518K","article-title":"Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids","volume":"5","author":"Romiza","year":"2015","journal-title":"RSC Adv."},{"key":"10.1016\/j.ecoenv.2020.111777_bib32","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1038\/labinvest.3700551","article-title":"Why microscopy will remain a cornerstone of surgical pathology","volume":"87","author":"Rosai","year":"2007","journal-title":"Lab. Investig."},{"key":"10.1016\/j.ecoenv.2020.111777_bib33","doi-asserted-by":"crossref","first-page":"502","DOI":"10.5858\/2005-129-502-HAOFSF","article-title":"Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology","volume":"129","author":"Titford","year":"2005","journal-title":"Arch. Pathol. Lab. Med."},{"key":"10.1016\/j.ecoenv.2020.111777_bib34","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1007\/s12551-018-0417-4","article-title":"Ionic liquid-based transmission electron microscopy for herpes simplex virus type 1","volume":"10","author":"Tsuda","year":"2018","journal-title":"Biophys. Rev."},{"key":"10.1016\/j.ecoenv.2020.111777_bib35","doi-asserted-by":"crossref","first-page":"2965","DOI":"10.1039\/C2JM15288F","article-title":"Plastic crystal phases with high proton conductivity","volume":"22","author":"Usman Ali Rana","year":"2012","journal-title":"J. Mater. Chem."},{"key":"10.1016\/j.ecoenv.2020.111777_bib36","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1002\/anie.200906610","article-title":"Long-term structural and chemical stability of DNA in hydrated ionic liquids","volume":"49","author":"Vijayaraghavan","year":"2010","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.ecoenv.2020.111777_bib37","doi-asserted-by":"crossref","first-page":"5363","DOI":"10.1002\/anie.200460575","article-title":"Ionic liquids as moderators in exothermic polymerization reactions","volume":"43","author":"Vijayaraghavan","year":"2004","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.ecoenv.2020.111777_bib38","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1039\/B910601D","article-title":"Biocompatibility of choline salts as crosslinking agents for collagen based biomaterials","volume":"46","author":"Vijayaraghavan","year":"2010","journal-title":"Chem. Commun."},{"key":"10.1016\/j.ecoenv.2020.111777_bib39","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1039\/C1CP22965F","article-title":"Structure and function of proteins in hydrated choline dihydrogen phosphate ionic liquid","volume":"14","author":"Weaver","year":"2012","journal-title":"Phys. Chem. Chem. Phys."},{"key":"10.1016\/j.ecoenv.2020.111777_bib40","first-page":"601","article-title":"Assessment of murine brain tissue shrinkage caused by different histological fixatives using magnetic resonance and computed tomography imaging","volume":"30","author":"Wehrl","year":"2015","journal-title":"Histol. Histopathol."},{"key":"10.1016\/j.ecoenv.2020.111777_bib41","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1476-069X-11-59","article-title":"Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde-free laboratory is possible","volume":"11","author":"Zanini","year":"2012","journal-title":"Environ. Health"}],"container-title":["Ecotoxicology and Environmental Safety"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651320316134?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651320316134?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T05:24:30Z","timestamp":1758950670000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147651320316134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":41,"alternative-id":["S0147651320316134"],"URL":"https:\/\/doi.org\/10.1016\/j.ecoenv.2020.111777","relation":{},"ISSN":["0147-6513"],"issn-type":[{"value":"0147-6513","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissues","name":"articletitle","label":"Article Title"},{"value":"Ecotoxicology and Environmental Safety","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ecoenv.2020.111777","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"111777"}}