{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T04:53:18Z","timestamp":1771649598673,"version":"3.50.1"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41967051"],"award-info":[{"award-number":["41967051"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42167059"],"award-info":[{"award-number":["42167059"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ecotoxicology and Environmental Safety"],"published-print":{"date-parts":[[2022,10]]},"DOI":"10.1016\/j.ecoenv.2022.114106","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T12:53:41Z","timestamp":1663851221000},"page":"114106","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Sulphur dioxide and fluoride co-exposure induce incisor hypomineralization and amelogenin upregulation via YAP\/RUNX2 signaling pathway"],"prefix":"10.1016","volume":"245","author":[{"given":"Junlin","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongtong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianghui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guohui","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenglong","family":"Tu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ecoenv.2022.114106_bib1","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1177\/08959374940080011201","article-title":"Strategies for improving the assessment of dental fluorosis: focus on chemical and biochemical aspects","volume":"8","author":"Aoba","year":"1994","journal-title":"Adv. Dent. Res."},{"key":"10.1016\/j.ecoenv.2022.114106_bib2","article-title":"Dental enamel development: proteinases and their enamel matrix substrates","volume":"2013","author":"Bartlett","year":"2013","journal-title":"ISRN Dent."},{"key":"10.1016\/j.ecoenv.2022.114106_bib3","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.ydbio.2004.02.012","article-title":"Runx2 mediates FGF signaling from epithelium to mesenchyme during tooth morphogenesis","volume":"270","author":"Berg","year":"2004","journal-title":"Dev. Biol."},{"key":"10.1016\/j.ecoenv.2022.114106_bib4","first-page":"36","article-title":"Experimental study on reducing fluorine and sulfur dioxide content in indoor air by mixing coal with lime","volume":"12","author":"Chen","year":"1997","journal-title":"Chin. J. Endem. Dis. Control"},{"key":"10.1016\/j.ecoenv.2022.114106_bib5","doi-asserted-by":"crossref","first-page":"9594","DOI":"10.1038\/s41598-018-27873-5","article-title":"Ablation of Runx2 in Ameloblasts Suppresses Enamel Maturation in Tooth Development","volume":"8","author":"Chu","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ecoenv.2022.114106_bib6","doi-asserted-by":"crossref","first-page":"435","DOI":"10.3389\/fphys.2017.00435","article-title":"Amelogenesis imperfecta; genes, proteins, and pathways","volume":"8","author":"Claire","year":"2017","journal-title":"Front. Physiol."},{"key":"10.1016\/j.ecoenv.2022.114106_bib7","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.catena.2017.01.013","article-title":"Runoff and erosion processes on bare slopes in the Karst Rocky Desertification Area","volume":"152","author":"Dai","year":"2017","journal-title":"Catena"},{"key":"10.1016\/j.ecoenv.2022.114106_bib8","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1159\/000327028","article-title":"Chronic fluoride toxicity: dental fluorosis","volume":"22","author":"Denbesten","year":"2011","journal-title":"Monogr. Oral. Sci."},{"key":"10.1016\/j.ecoenv.2022.114106_bib9","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1177\/00220345860650101401","article-title":"Effects of fluoride on protein secretion and removal during enamel development in the rat","volume":"65","author":"DenBesten","year":"1986","journal-title":"J. Dent. Res."},{"key":"10.1016\/j.ecoenv.2022.114106_bib10","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S0003-9969(02)00117-6","article-title":"Effects of fluoride on rat dental enamel matrix proteinases","volume":"47","author":"DenBesten","year":"2002","journal-title":"Arch. Oral. Biol."},{"key":"10.1016\/j.ecoenv.2022.114106_bib11","doi-asserted-by":"crossref","first-page":"18","DOI":"10.3390\/molecules25184214","article-title":"Identification of key functional motifs of native amelogenin protein for dental enamel remineralisation","volume":"25","author":"Dissanayake","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.ecoenv.2022.114106_bib12","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1242\/dev.126.13.2911","article-title":"Cbfa1 is required for epithelial-mesenchymal interactions regulating tooth development in mice","volume":"126","author":"D'Souza","year":"1999","journal-title":"Development"},{"key":"10.1016\/j.ecoenv.2022.114106_bib13","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1038\/nature10137","article-title":"Role of YAP\/TAZ in mechanotransduction","volume":"474","author":"Dupont","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.ecoenv.2022.114106_bib14","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.archoralbio.2017.08.008","article-title":"Structural, mechanical and chemical evaluation of molar-incisor hypomineralization-affected enamel: a systematic review","volume":"83","author":"Elhennawy","year":"2017","journal-title":"Arch. Oral. Biol."},{"key":"10.1016\/j.ecoenv.2022.114106_bib15","doi-asserted-by":"crossref","first-page":"4458","DOI":"10.3390\/ijms21124458","article-title":"Tooth enamel and its dynamic protein matrix","volume":"21","author":"Gil-Bona","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.ecoenv.2022.114106_bib16","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.17219\/acem\/128227","article-title":"The impact of environmental air pollution on the prevalence of molar incisor hypomineralization in schoolchildren: a cross-sectional study","volume":"29","author":"G\u00f3dkowska","year":"2020","journal-title":"Adv. Clin. Exp. Med."},{"key":"10.1016\/j.ecoenv.2022.114106_bib17","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1177\/0022034514564186","article-title":"Amelogenins as potential buffers during secretory-stage amelogenesis","volume":"94","author":"Guo","year":"2015","journal-title":"J. Dent. Res.: Off. Publ. Int. Assoc. Dent. Res."},{"key":"10.1016\/j.ecoenv.2022.114106_bib18","doi-asserted-by":"crossref","first-page":"3398","DOI":"10.1073\/pnas.79.11.3398","article-title":"P1 site-specific recombination: nucleotide sequence of the recombining sites","volume":"79","author":"Hoess","year":"1982","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.ecoenv.2022.114106_bib19","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1021\/ed043p202","article-title":"The photochemical reaction of xenon with fluorine at room temperature: a demonstration of the reactivity of xenon","volume":"43","author":"Holloway","year":"1966","journal-title":"J. Chem. Educ."},{"key":"10.1016\/j.ecoenv.2022.114106_bib20","first-page":"1","article-title":"Analysis on the status que and causes of fluorine and acid pollution around coal-rich areas in China","volume":"5","author":"Hong","year":"2020","journal-title":"J. Min. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2022.114106_bib21","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0925-4773(98)00232-9","article-title":"Expression of core binding factor Osf2\/Cbfa-1 and bone sialoprotein in tooth development","volume":"81","author":"Jiang","year":"1999","journal-title":"Mech. Dev."},{"key":"10.1016\/j.ecoenv.2022.114106_bib22","first-page":"199","article-title":"Effects of Overdosed Fluoride on Rat's Incisor Expression of Matrixmetalloproteinase-20 and Tissue Inhibitors of Metalloproteinase-2","volume":"24","author":"Jing","year":"2006","journal-title":"Hua Xi Kou Qiang Yi Xue Za Zhi"},{"key":"10.1016\/j.ecoenv.2022.114106_bib23","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1007\/s00204-020-02687-5","article-title":"Principles of fluoride toxicity and the cellular response: a review","volume":"94","author":"Johnston","year":"2020","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.ecoenv.2022.114106_bib24","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.bone.2005.10.026","article-title":"Type II\/III Runx2\/Cbfa1 is required for tooth germ development","volume":"38","author":"Kobayashi","year":"2006","journal-title":"Bone"},{"key":"10.1016\/j.ecoenv.2022.114106_bib25","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1152\/physrev.00030.2016","article-title":"Dental enamel formation and implications for oral health and disease","volume":"97","author":"Lacruz","year":"2017","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.ecoenv.2022.114106_bib26","doi-asserted-by":"crossref","unstructured":"Lamar, J.M., Stern, P., Liu, H., Schindler, J.W., Jiang, Z., Hynes, R.O., 2012. The Hippo pathway target, YAP, promotes metastasis through its TEAD-interaction domain. Proceedings of the National Academy of Sciences. \u3008https:\/\/doi.org\/10.1073\/pnas.1212021109\u3009.","DOI":"10.1073\/pnas.1212021109"},{"key":"10.1016\/j.ecoenv.2022.114106_bib27","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.toxlet.2017.12.020","article-title":"Sulfur dioxide exposure enhances Th2 inflammatory responses via activating STAT6 pathway in asthmatic mice","volume":"285","author":"Li","year":"2017","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.ecoenv.2022.114106_bib28","first-page":"2081","article-title":"Research progress in the regulation of pathogenicity by Runx2","volume":"24","author":"Li","year":"2018","journal-title":"Med. Recapitul."},{"key":"10.1016\/j.ecoenv.2022.114106_bib29","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1007\/s12011-018-1325-y","article-title":"NaF reduces KLK4 gene expression by decreasing Foxo1 in LS8 cells","volume":"186","author":"Li","year":"2018","journal-title":"Biol. Trace Elem. Res."},{"key":"10.1016\/j.ecoenv.2022.114106_bib30","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s10456-021-09814-5","article-title":"Extracellular matrix stiffness controls VEGF165 secretion and neuroblastoma angiogenesis via the YAP\/RUNX2\/SRSF1 axis","volume":"25","author":"Li","year":"2022","journal-title":"Angiogenesis"},{"key":"10.1016\/j.ecoenv.2022.114106_bib31","first-page":"148","article-title":"Simulation experiment of soot fluorosis and the effect of sulfur dioxide on fluorosis","volume":"3","author":"Liang","year":"1993","journal-title":"Health Res."},{"key":"10.1016\/j.ecoenv.2022.114106_bib32","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1007\/s11434-011-4560-6","article-title":"Potential release of hydrogen fluoride from domestic coal in endemic fluorosis area in Guizhou, China","volume":"56","author":"Liang","year":"2011","journal-title":"Chin. Sci. Bull."},{"key":"10.1016\/j.ecoenv.2022.114106_bib33","article-title":"Sodium fluoride and sulfur dioxide derivatives induce TGF-\u03b21-mediated NBCe1 downregulation causing acid\u2013base disorder of LS8","author":"Lv","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.ecoenv.2022.114106_bib34","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.matbio.2007.09.006","article-title":"The anion exchanger Ae2 is required for enamel maturation in mouse teeth","volume":"27","author":"Lyaruu","year":"2008","journal-title":"Matrix Biol."},{"key":"10.1016\/j.ecoenv.2022.114106_bib35","article-title":"Amelogenesis: Transformation of a protein-mineral matrix into tooth enamel","volume":"213","author":"Mirali","year":"2021","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.ecoenv.2022.114106_bib36","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1590\/0103-6440201600424","article-title":"Peptide Characterization of Mature Fluorotic and Control Human Enamel","volume":"27","author":"Porto","year":"2016","journal-title":"Braz. Dent. J."},{"key":"10.1016\/j.ecoenv.2022.114106_bib37","first-page":"325","article-title":"Delayed dental maturation in cleidocranial dysplasia","volume":"65","author":"Shaikh","year":"1998","journal-title":"Asdc J. Dent. Child"},{"key":"10.1016\/j.ecoenv.2022.114106_bib38","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0010895","article-title":"The acid test of fluoride: how pH modulates toxicity","volume":"5","author":"Sharma","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.ecoenv.2022.114106_bib39","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1177\/0022034511398868","article-title":"Assessment of dental fluorosis in Mmp20 +\/- mice","volume":"90","author":"Sharma","year":"2011","journal-title":"J. Dent. Res."},{"key":"10.1016\/j.ecoenv.2022.114106_bib40","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1080\/03008200290001159","article-title":"Expression, structure, and function of enamel proteinases","volume":"43","author":"Simmer","year":"2002","journal-title":"Connect. Tissue Res."},{"key":"10.1016\/j.ecoenv.2022.114106_bib41","unstructured":"Strober, R., 2013. How Bad Is Pollution for Your Teeth? \u3008https:\/\/www.countryclubdentistry.com\/blog\/bad-pollution-teeth\/\u3009."},{"key":"10.1016\/j.ecoenv.2022.114106_bib42","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1177\/0022034514545629","article-title":"Fluoride affects enamel protein content via TGF-\u03b21-mediated KLK4 inhibition","volume":"93","author":"Suzuki","year":"2014","journal-title":"J. Dent. Res."},{"key":"10.1016\/j.ecoenv.2022.114106_bib43","first-page":"304","article-title":"Challenges of fluoride pollution in environment: mechanisms and pathological significance of toxicity \u2013 a review","author":"Sw","year":"2022","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.ecoenv.2022.114106_bib44","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1159\/000151431","article-title":"Lysosomal protease expression in mature enamel","volume":"189","author":"Tye","year":"2009","journal-title":"Cells Tissues Organs"},{"key":"10.1016\/j.ecoenv.2022.114106_bib45","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1177\/0022034510390043","article-title":"Fluoride does not inhibit enamel protease activity","volume":"90","author":"Tye","year":"2011","journal-title":"J. Dent. Res."},{"key":"10.1016\/j.ecoenv.2022.114106_bib46","first-page":"546","article-title":"Effect of magnesium and selenium on the expression of matrix metalloproteinases-20 and kallikrein 4 in fluorosis mice","volume":"51","author":"Wang","year":"2016","journal-title":"Zhonghua kou qiang yi xue za zhi"},{"key":"10.1016\/j.ecoenv.2022.114106_bib47","first-page":"226","article-title":"Determinants and mechanisms of enamel fluorosis","volume":"205","author":"Whitford","year":"1997","journal-title":"Ciba Found. Symp."},{"key":"10.1016\/j.ecoenv.2022.114106_bib48","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/j.1600-0722.2011.00866.x","article-title":"Enamel proteins and proteases in Mmp20 and Klk4 null and double-null mice","volume":"119","author":"Yamakoshi","year":"2011","journal-title":"Eur. J. Oral. Sci."},{"key":"10.1016\/j.ecoenv.2022.114106_bib49","first-page":"188","article-title":"UV\u2013visible absorption spectral properties of sulfur dioxide and its derivatives","volume":"32","author":"Yang","year":"2013","journal-title":"Environ. Chem."},{"key":"10.1016\/j.ecoenv.2022.114106_bib50","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.scitotenv.2019.01.026","article-title":"Properties of particulate matter and gaseous pollutants in Shandong, China: Daily fluctuation, influencing factors, and spatiotemporal distribution","volume":"660","author":"Yao","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2022.114106_bib51","first-page":"1094","article-title":"Occurrence and geological genesis of pyrite in permian high sulfur coal in Guizhou Province","volume":"43","author":"Zhang","year":"2018","journal-title":"China J. China Coal Soc."},{"key":"10.1016\/j.ecoenv.2022.114106_bib52","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.matbio.2007.06.002","article-title":"JNK\/c-Jun signaling pathway mediates the fluoride-induced down-regulation of MMP-20 in vitro","volume":"26","author":"Zhang","year":"2007","journal-title":"Matrix Biol. J. Int. Soc. Matrix Biol."},{"key":"10.1016\/j.ecoenv.2022.114106_bib53","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1600-0722.2006.00303.x","article-title":"Fluoride down-regulates the expression of matrix metalloproteinase-20 in human fetal tooth ameloblast-lineage cells in vitro","volume":"114","author":"Zhang","year":"2010","journal-title":"Eur. J. Oral. Sci."},{"key":"10.1016\/j.ecoenv.2022.114106_bib54","unstructured":"Zhu, Q.L., 2005. Study of Cbf\u03b11 on Amelogenin transcriptional regulation. Fourth Military Medical University."}],"container-title":["Ecotoxicology and Environmental Safety"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651322009460?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651322009460?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T05:58:11Z","timestamp":1758952691000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147651322009460"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":54,"alternative-id":["S0147651322009460"],"URL":"https:\/\/doi.org\/10.1016\/j.ecoenv.2022.114106","relation":{},"ISSN":["0147-6513"],"issn-type":[{"value":"0147-6513","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Sulphur dioxide and fluoride co-exposure induce incisor hypomineralization and amelogenin upregulation via YAP\/RUNX2 signaling pathway","name":"articletitle","label":"Article Title"},{"value":"Ecotoxicology and Environmental Safety","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ecoenv.2022.114106","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"114106"}}