{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T02:24:01Z","timestamp":1792549441904,"version":"4.5.0"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2022YFC3703000"],"award-info":[{"award-number":["2022YFC3703000"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2022YFC3702603"],"award-info":[{"award-number":["2022YFC3702603"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2024YFC3044700"],"award-info":[{"award-number":["2024YFC3044700"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ecotoxicology and Environmental Safety"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.ecoenv.2024.117545","type":"journal-article","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T13:30:32Z","timestamp":1736343032000},"page":"117545","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Traffic-related air pollution (TRAP) exposure, lung function, airway inflammation and expiratory microbiota: A randomized crossover study"],"prefix":"10.1016","volume":"289","author":[{"given":"Rongrong","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhao","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanshan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Te","family":"Ba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baorong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2580-8052","authenticated-orcid":false,"given":"Yongjie","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ecoenv.2024.117545_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2022.107262","article-title":"Long-term exposure to traffic-related air pollution and selected health outcomes: a systematic review and meta-analysis","volume":"164","author":"Boogaard","year":"2022","journal-title":"Environ. Int."},{"key":"10.1016\/j.ecoenv.2024.117545_bib2","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.envint.2018.01.028","article-title":"Traffic related air pollution and development and persistence of asthma and low lung function","volume":"113","author":"Bowatte","year":"2018","journal-title":"Environ. Int."},{"key":"10.1016\/j.ecoenv.2024.117545_bib3","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1164\/rccm.201407-1314OC","article-title":"Timing and duration of traffic-related air pollution exposure and the risk for childhood wheeze and asthma","volume":"192","author":"Brunst","year":"2015","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.ecoenv.2024.117545_bib4","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2020.570484","article-title":"Fine particulate matter exposure alters pulmonary microbiota composition and aggravates pneumococcus-induced lung pathogenesis","volume":"8","author":"Chen","year":"2020","journal-title":"Front Cell Dev. Biol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib5","doi-asserted-by":"crossref","first-page":"2846","DOI":"10.1111\/all.14438","article-title":"Integration of metagenomics-metabolomics reveals specific signatures and functions of airway microbiota in mite-sensitized childhood asthma","volume":"75","author":"Chiu","year":"2020","journal-title":"Allergy"},{"key":"10.1016\/j.ecoenv.2024.117545_bib6","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41586-019-1517-4","article-title":"Organization and regulation of gene transcription","volume":"573","author":"Cramer","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.ecoenv.2024.117545_bib7","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1513\/AnnalsATS.201501-029OC","article-title":"Spatial variation in the healthy human lung microbiome and the adapted island model of lung biogeography","volume":"12","author":"Dickson","year":"2015","journal-title":"Ann. Am. Thorac. Soc."},{"key":"10.1016\/j.ecoenv.2024.117545_bib8","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1038\/s41587-020-0548-6","article-title":"PICRUSt2 for prediction of metagenome functions","volume":"38","author":"Douglas","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2021.127359","article-title":"Dynamic molecular choreography induced by traffic exposure: a randomized, crossover trial using multi-omics profiling","volume":"424","author":"Du","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.ecoenv.2024.117545_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.157968","article-title":"Traffic-related air pollution and genome-wide DNA methylation: a randomized, crossover trial","volume":"850","author":"Du","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2024.117545_bib11","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1183\/13993003.01176-2015","article-title":"Traffic-related air pollution and alveolar nitric oxide in southern California children","volume":"47","author":"Eckel","year":"2016","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.ecoenv.2024.117545_bib12","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.00515-17","article-title":"Microbiota in exhaled breath condensate and the lung","volume":"83","author":"Glendinning","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib13","doi-asserted-by":"crossref","first-page":"e70","DOI":"10.1164\/rccm.201908-1590ST","article-title":"Standardization of spirometry 2019 update. An official American thoracic society and european respiratory society technical statement","volume":"200","author":"Graham","year":"2019","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.ecoenv.2024.117545_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2023.132138","article-title":"Source apportionment of consumed volatile organic compounds in the atmosphere","volume":"459","author":"Gu","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.ecoenv.2024.117545_bib15","doi-asserted-by":"crossref","DOI":"10.1097\/EE9.0000000000000066","article-title":"A randomized, double-blind, crossover intervention study of traffic-related air pollution and airway inflammation in healthy adults","volume":"3","author":"Han","year":"2019","journal-title":"Environ. Epidemiol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib16","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1183\/09031936.05.00029705","article-title":"Exhaled breath condensate: methodological recommendations and unresolved questions","volume":"26","author":"Horvath","year":"2005","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.ecoenv.2024.117545_bib17","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s12940-020-00678-8","article-title":"Exposure to traffic-related air pollution and changes in exhaled nitric oxide and DNA methylation in arginase and nitric oxide synthase in children with asthma","volume":"20","author":"Ji","year":"2021","journal-title":"Environ. Health"},{"key":"10.1016\/j.ecoenv.2024.117545_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.154974","article-title":"Lung microbiome and transcriptome reveal mechanisms underlying PM(2.5) induced pulmonary fibrosis","volume":"831","author":"Jia","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2024.117545_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.137432","article-title":"PM2.5 exposure perturbs lung microbiome and its metabolic profile in mice","volume":"721","author":"Li","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2024.117545_bib20","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.04594-2020","article-title":"Long-term air pollution and road traffic noise exposure and COPD: the Danish Nurse Cohort","volume":"58","author":"Liu","year":"2021","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.ecoenv.2024.117545_bib21","article-title":"Controlled human exposure to diesel exhaust: results illuminate health effects of traffic-related air pollution and inform future directions","volume":"19","author":"Long","year":"2022","journal-title":"Part. Fibre Toxicol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib22","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.envint.2016.10.011","article-title":"Acute respiratory response to traffic-related air pollution during physical activity performance","volume":"97","author":"Matt","year":"2016","journal-title":"Environ. Int."},{"key":"10.1016\/j.ecoenv.2024.117545_bib23","first-page":"279","article-title":"Air pollution and cardiovascular disease: car sick","volume":"116","author":"Miller","year":"2020","journal-title":"Cardiovasc. Res."},{"key":"10.1016\/j.ecoenv.2024.117545_bib24","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1016\/S0140-6736(20)30752-2","article-title":"Global burden of 87 risk factors in 204 countries and territories, 1990\u20132019: a systematic analysis for the Global Burden of Disease Study 2019","volume":"396","author":"Murray","year":"2020","journal-title":"Lancet"},{"key":"10.1016\/j.ecoenv.2024.117545_bib25","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/s41579-022-00821-x","article-title":"The dynamic lung microbiome in health and disease","volume":"21","author":"Natalini","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib26","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1183\/09059180.00000714","article-title":"Volatile organic compounds and risk of asthma and allergy: a systematic review","volume":"24","author":"Nurmatov","year":"2015","journal-title":"Eur. Respir. Rev."},{"key":"10.1016\/j.ecoenv.2024.117545_bib27","doi-asserted-by":"crossref","DOI":"10.3390\/jcm10122697","article-title":"Exhaled breath condensate-a non-invasive approach for diagnostic methods in asthma","volume":"10","author":"Polomska","year":"2021","journal-title":"J. Clin. Med"},{"key":"10.1016\/j.ecoenv.2024.117545_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2020.115987","article-title":"Subchronic exposure to concentrated ambient PM2.5 perturbs gut and lung microbiota as well as metabolic profiles in mice","volume":"272","author":"Ran","year":"2021","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.ecoenv.2024.117545_bib29","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1038\/nbt.2170","article-title":"FLASH assembly of TALENs for high-throughput genome editing","volume":"30","author":"Reyon","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib30","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.2584","article-title":"VSEARCH: a versatile open source tool for metagenomics","volume":"4","author":"Rognes","year":"2016","journal-title":"PeerJ"},{"key":"10.1016\/j.ecoenv.2024.117545_bib31","doi-asserted-by":"crossref","DOI":"10.1186\/s12866-016-0803-7","article-title":"Household air pollution and the lung microbiome of healthy adults in Malawi: a cross-sectional study","volume":"16","author":"Rylance","year":"2016","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.ecoenv.2024.117545_bib32","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.envres.2014.05.004","article-title":"Exposure to traffic pollution, acute inflammation and autonomic response in a panel of car commuters","volume":"133","author":"Sarnat","year":"2014","journal-title":"Environ. Res."},{"key":"10.1016\/j.ecoenv.2024.117545_bib33","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1111\/cea.12373","article-title":"The role of non-invasive biomarkers in detecting acute respiratory effects of traffic-related air pollution","volume":"44","author":"Scarpa","year":"2014","journal-title":"Clin. Exp. Allergy"},{"key":"10.1016\/j.ecoenv.2024.117545_bib34","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib35","doi-asserted-by":"crossref","first-page":"2739","DOI":"10.1007\/s00018-020-03452-8","article-title":"The lung microbiota: role in maintaining pulmonary immune homeostasis and its implications in cancer development and therapy","volume":"77","author":"Sommariva","year":"2020","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.ecoenv.2024.117545_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2020.122817","article-title":"Inflammatory cytokines and DNA methylation in healthy young adults exposure to fine particulate matter: a randomized, double-blind crossover trial of air filtration","volume":"398","author":"Sun","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.ecoenv.2024.117545_bib37","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.chest.2021.10.020","article-title":"Effects of traffic-related air pollution on exercise endurance, dyspnea, and cardiorespiratory responses in health and COPD: a randomized, placebo-controlled, crossover trial","volume":"161","author":"Syed","year":"2022","journal-title":"Chest"},{"key":"10.1016\/j.ecoenv.2024.117545_bib38","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.3389\/fimmu.2018.01873","article-title":"The role of histamine and histamine receptors in mast cell-mediated allergy and inflammation: the hunt for new therapeutic targets","volume":"9","author":"Thangam","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib39","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1007\/s12016-018-8719-7","article-title":"The human microbiota and asthma","volume":"57","author":"Ver Heul","year":"2019","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib40","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1007\/s11427-017-9151-1","article-title":"Role of microbiota on lung homeostasis and diseases","volume":"60","author":"Wang","year":"2017","journal-title":"Sci. China Life Sci."},{"key":"10.1016\/j.ecoenv.2024.117545_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2021.127463","article-title":"Traffic-related air pollution associated pulmonary pathophysiologic changes and cardiac injury in elderly patients with COPD","volume":"424","author":"Wang","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.ecoenv.2024.117545_bib42","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2022.113745","article-title":"Short-term effects of the toxic component of traffic-related air pollution (TRAP) on lung function in healthy adults using a powered air purifying respirator (PAPR)","volume":"214","author":"Xu","year":"2022","journal-title":"Environ. Res."},{"key":"10.1016\/j.ecoenv.2024.117545_bib43","doi-asserted-by":"crossref","DOI":"10.1093\/bioinformatics\/btad470","article-title":"ggpicrust2: an R package for PICRUSt2 predicted functional profile analysis and visualization","volume":"39","author":"Yang","year":"2023","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ecoenv.2024.117545_bib44","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jes.2021.01.035","article-title":"Characteristics, sources and health risks assessment of VOCs in Zhengzhou, China during haze pollution season","volume":"108","author":"Zhang","year":"2021","journal-title":"J. Environ. Sci."},{"key":"10.1016\/j.ecoenv.2024.117545_bib45","doi-asserted-by":"crossref","first-page":"6282","DOI":"10.1021\/acs.est.2c00688","article-title":"Insights into the Profile of the Human Expiratory Microbiota and Its Associations with Indoor Microbiotas","volume":"56","author":"Zhang","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib46","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1165\/rcmb.2022-0208TR","article-title":"The human respiratory microbiome: current understandings and future directions","volume":"68","author":"Zhao","year":"2023","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"10.1016\/j.ecoenv.2024.117545_bib47","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2022.137489","article-title":"Environmental and human health impacts of volatile organic compounds: a perspective review","volume":"313","author":"Zhou","year":"2023","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2024.117545_bib48","doi-asserted-by":"crossref","first-page":"57002","DOI":"10.1289\/EHP11139","article-title":"Respiratory effects of traffic-related air pollution: a randomized, crossover analysis of lung function, airway metabolome, and biomarkers of airway injury","volume":"131","author":"Zhu","year":"2023","journal-title":"Environ. Health Perspect."}],"container-title":["Ecotoxicology and Environmental Safety"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S014765132401621X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S014765132401621X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T08:45:33Z","timestamp":1762245933000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S014765132401621X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":48,"alternative-id":["S014765132401621X"],"URL":"https:\/\/doi.org\/10.1016\/j.ecoenv.2024.117545","relation":{},"ISSN":["0147-6513"],"issn-type":[{"value":"0147-6513","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Traffic-related air pollution (TRAP) exposure, lung function, airway inflammation and expiratory microbiota: A randomized crossover study","name":"articletitle","label":"Article Title"},{"value":"Ecotoxicology and Environmental Safety","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ecoenv.2024.117545","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117545"}}