{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:39:18Z","timestamp":1790746758429,"version":"4.1.0"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515012252"],"award-info":[{"award-number":["2024A1515012252"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022B1515130003"],"award-info":[{"award-number":["2022B1515130003"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20240"],"award-info":[{"award-number":["U23A20240"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32102718"],"award-info":[{"award-number":["32102718"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ecotoxicology and Environmental Safety"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.ecoenv.2025.118489","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T12:35:46Z","timestamp":1749731746000},"page":"118489","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Mechanistic insights into deoxynivalenol-Induced hepatic cholestasis via IRE1\u03b1\/HNF1\u03b1\/FXR signaling dysregulation in mice"],"prefix":"10.1016","volume":"301","author":[{"given":"Yuting","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruqin","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianqing","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiqiang","family":"Mu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4010-4416","authenticated-orcid":false,"given":"Jikai","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqun","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"7916","key":"10.1016\/j.ecoenv.2025.118489_bib1","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1038\/s41586-022-04845-4","article-title":"Structure of the bile acid transporter and HBV receptor NTCP","volume":"606","author":"Asami","year":"2022","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib2","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-12629-4","article-title":"The FXR agonist, obeticholic acid, suppresses HCC proliferation & metastasis: role of IL-6\/STAT3 signalling pathway","volume":"7","author":"Attia","year":"2017","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib3","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-68712-w","article-title":"The food contaminant deoxynivalenol provokes metabolic impairments resulting in non-alcoholic fatty liver (NAFL) in mice","volume":"10","author":"Barbouche","year":"2020","journal-title":"Sci. Rep."},{"issue":"8","key":"10.1016\/j.ecoenv.2025.118489_bib4","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/nm.1853","article-title":"Hepatocyte-specific ablation of Foxa2 alters bile acid homeostasis and results in endoplasmic reticulum stress","volume":"14","author":"Bochkis","year":"2008","journal-title":"Nat. Med"},{"issue":"2","key":"10.1016\/j.ecoenv.2025.118489_bib5","doi-asserted-by":"crossref","first-page":"81","DOI":"10.3390\/toxins10020081","article-title":"Natural occurrence of Nivalenol, Deoxynivalenol, and Deoxynivalenol-3-Glucoside in Polish Winter Wheat","volume":"10","author":"Bryla","year":"2018","journal-title":"Toxins"},{"issue":"11","key":"10.1016\/j.ecoenv.2025.118489_bib6","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1016\/j.molp.2023.09.010","article-title":"TBtools-II: A \"one for all, all for one\"bioinformatics platform for biological big-data mining","volume":"16","author":"Chen","year":"2023","journal-title":"Mol. Plant"},{"key":"10.1016\/j.ecoenv.2025.118489_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.steroids.2020.108757","article-title":"Enterohepatic circulation of bile acids and their emerging roles on glucolipid metabolism","volume":"165","author":"Chen","year":"2021","journal-title":"Steroids"},{"key":"10.1016\/j.ecoenv.2025.118489_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.fct.2022.113289","article-title":"Exposure assessment of urinary deoxynivalenol in pregnant women in Wuhan, China","volume":"167","author":"Chen","year":"2022","journal-title":"Food Chem. Toxicol."},{"issue":"9","key":"10.1016\/j.ecoenv.2025.118489_bib9","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1038\/s42255-022-00631-8","article-title":"Adipocyte IRE1\u03b1 promotes PGC1\u03b1 mRNA decay and restrains adaptive thermogenesis","volume":"4","author":"Chen","year":"2022","journal-title":"Nat. Metab."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib10","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1053\/gast.2001.25503","article-title":"The orphan nuclear receptor, shp, mediates bile acid-induced inhibition of the rat bile acid transporter, ntcp","volume":"121","author":"Denson","year":"2001","journal-title":"Gastroenterology"},{"key":"10.1016\/j.ecoenv.2025.118489_bib11","unstructured":"DSM, 2023. DSM World Mycotoxin Survey. https:\/\/www.dsm.com\/content\/dam\/dsm\/anh\/en\/documents\/REP_MTXsurvey_Q2_2023_EN_0723_AUE%20(2).pdf."},{"issue":"12","key":"10.1016\/j.ecoenv.2025.118489_bib12","doi-asserted-by":"crossref","first-page":"4343","DOI":"10.3390\/nu13124343","article-title":"FXR, a key regulator of lipid metabolism, is inhibited by ER stress-mediated activation of JNK and p38 MAPK in large yellow Croakers (Larimichthys crocea) fed high fat diets","volume":"13","author":"Du","year":"2021","journal-title":"Nutrients"},{"issue":"2","key":"10.1016\/j.ecoenv.2025.118489_bib13","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1002\/hep.32101","article-title":"Pharmacologic activation of hepatic farnesoid X receptor prevents parenteral nutrition-associated cholestasis in mice","volume":"75","author":"El Kasmi","year":"2022","journal-title":"Hepatology"},{"issue":"11","key":"10.1016\/j.ecoenv.2025.118489_bib14","article-title":"Non-steroidal FXR agonist cilofexor improves cholestatic liver injury in the Mdr2-\/- mouse model of sclerosing cholangitis","volume":"5","author":"Fuchs","year":"2023","journal-title":"JHEP Rep."},{"issue":"6","key":"10.1016\/j.ecoenv.2025.118489_bib15","doi-asserted-by":"crossref","first-page":"3284","DOI":"10.1002\/hep.32071","article-title":"NF-\u03baB Regulation of LRH-1 and ABCG5\/8 potentiates phytosterol role in the pathogenesis of parenteral nutrition-associated cholestasis","volume":"74","author":"Ghosh","year":"2021","journal-title":"Hepatology"},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib16","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.jhep.2012.08.002","article-title":"Bile acid transporters and regulatory nuclear receptors in the liver and beyond","volume":"58","author":"Halilbasic","year":"2013","journal-title":"J. Hepatol."},{"issue":"4","key":"10.1016\/j.ecoenv.2025.118489_bib17","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.cmet.2017.03.007","article-title":"Farnesoid X receptor regulation of the NLRP3 inflammasome underlies cholestasis-associated sepsis","volume":"25","author":"Hao","year":"2017","journal-title":"Cell Metab."},{"issue":"8","key":"10.1016\/j.ecoenv.2025.118489_bib18","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1194\/jlr.R049437","article-title":"Key discoveries in bile acid chemistry and biology and their clinical applications: history of the last eight decades","volume":"55","author":"Hofmann","year":"2014","journal-title":"J. Lipid Res."},{"issue":"6","key":"10.1016\/j.ecoenv.2025.118489_bib19","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1080\/10408398.2021.1954598","article-title":"The toxicity mechanisms of DON to humans and animals and potential biological treatment strategies","volume":"63","author":"Hou","year":"2023","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"10.1016\/j.ecoenv.2025.118489_bib20","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2022.1022809","article-title":"Involvement of endoplasmic reticulum stress in rifampicin-induced liver injury","volume":"13","author":"Hou","year":"2022","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.ecoenv.2025.118489_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2023.114705","article-title":"Dietary taurine supplementation counteracts deoxynivalenol-induced liver injury via alleviating oxidative stress, mitochondrial dysfunction, apoptosis, and inflammation in piglets","volume":"253","author":"Ji","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"issue":"12","key":"10.1016\/j.ecoenv.2025.118489_bib22","doi-asserted-by":"crossref","first-page":"6660","DOI":"10.1021\/acs.jafc.4c00556","article-title":"Ferritinophagy Is Critical for Deoxynivalenol-Induced Liver Injury in Mice","volume":"72","author":"Junze","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.ecoenv.2025.118489_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.molmet.2023.101846","article-title":"Inhibiting the endoplasmic reticulum stress response enhances the effect of doxorubicin by altering the lipid metabolism of liver cancer cells","volume":"79","author":"Kopsida","year":"2024","journal-title":"Mol. Metab."},{"issue":"4","key":"10.1016\/j.ecoenv.2025.118489_bib24","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.1074\/jbc.M209525200","article-title":"The farnesoid X-receptor is an essential regulator of cholesterol homeostasis","volume":"278","author":"Lambert","year":"2003","journal-title":"J. Biol. Chem."},{"issue":"2","key":"10.1016\/j.ecoenv.2025.118489_bib25","doi-asserted-by":"crossref","first-page":"152","DOI":"10.21037\/hbsn.2019.09.03","article-title":"Bile acid-based therapies for non-alcoholic steatohepatitis and alcoholic liver disease","volume":"9","author":"Li","year":"2020","journal-title":"Hepatobiliary Surg. Nutr."},{"key":"10.1016\/j.ecoenv.2025.118489_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcp.2020.113868","article-title":"Lactobacillus rhamnosus GG supplementation modulates the gut microbiota to promote butyrate production, protecting against deoxynivalenol exposure in nude mice","volume":"175","author":"Lin","year":"2020","journal-title":"Biochem. Pharmacol."},{"issue":"3","key":"10.1016\/j.ecoenv.2025.118489_bib27","first-page":"1455","article-title":"GW4064 attenuates lipopolysaccharide\u2011induced hepatic inflammation and apoptosis through inhibition of the Toll\u2011like receptor 4\u2011mediated p38 mitogen\u2011activated protein kinase signaling pathway in mice","volume":"41","author":"Liu","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.ecoenv.2025.118489_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.fct.2023.113751","article-title":"Aflatoxin B1 induces liver injury by disturbing gut microbiota-bile acid-FXR axis in mice","volume":"176","author":"Liu","year":"2023","journal-title":"Food Chem. Toxicol."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib29","doi-asserted-by":"crossref","DOI":"10.1080\/2162402X.2023.2217024","article-title":"FXR agonist GW4064 enhances anti-PD-L1 immunotherapy in colorectal cancer","volume":"12","author":"Lu","year":"2023","journal-title":"OncoImmunology"},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib30","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/s41467-023-44416-3","article-title":"A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice","volume":"15","author":"Lu","year":"2024","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib31","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1042\/BST20210658","article-title":"Bile acid metabolism and FXR-mediated effects in human cholestatic liver disorders","volume":"50","author":"Molinaro","year":"2022","journal-title":"Biochem. Soc. Trans."},{"issue":"9972","key":"10.1016\/j.ecoenv.2025.118489_bib32","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/S0140-6736(14)61933-4","article-title":"Farnesoid X nuclear receptor ligand obeticholic acid for non-cirrhotic, non-alcoholic steatohepatitis (FLINT): a multicentre, randomised, placebo-controlled trial","volume":"385","author":"Neuschwander-Tetri","year":"2015","journal-title":"Lancet"},{"issue":"6","key":"10.1016\/j.ecoenv.2025.118489_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.clinre.2021.101641","article-title":"Regulatory mechanisms of the bile salt export pump (BSEP\/ABCB11) and its role in related diseases","volume":"45","author":"Ren","year":"2021","journal-title":"Clin. Res. Hepatol. Gastroenterol."},{"key":"10.1016\/j.ecoenv.2025.118489_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2019.108909","article-title":"Adolescent exposure to environmental level of PCBs (Aroclor 1254) induces non-alcoholic fatty liver disease in male mice","volume":"181","author":"Ruan","year":"2020","journal-title":"Environ. Res."},{"issue":"2","key":"10.1016\/j.ecoenv.2025.118489_bib35","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.cmet.2013.01.003","article-title":"Gut microbiota regulates bile acid metabolism by reducing the levels of Tauro-beta-muricholic Acid, a naturally occurring FXR antagonist","volume":"17","author":"Sayin","year":"2013","journal-title":"Cell Metab."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib36","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1186\/s12974-023-02903-w","article-title":"Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1\u03b1\/ASK1\/JNK signaling pathway in mice with traumatic brain injury","volume":"20","author":"Shi","year":"2023","journal-title":"J. Neuroinflamm."},{"issue":"18","key":"10.1016\/j.ecoenv.2025.118489_bib37","doi-asserted-by":"crossref","first-page":"14066","DOI":"10.3390\/ijms241814066","article-title":"Unfolded protein response signaling in liver disorders: a 2023 updated review","volume":"24","author":"Shreya","year":"2023","journal-title":"Int. J. Mol. Sci."},{"issue":"10","key":"10.1016\/j.ecoenv.2025.118489_bib38","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1007\/s00204-022-03337-8","article-title":"Toxicokinetics and metabolism of deoxynivalenol in animals and humans","volume":"96","author":"Sun","year":"2022","journal-title":"Arch. Toxicol."},{"issue":"4","key":"10.1016\/j.ecoenv.2025.118489_bib39","doi-asserted-by":"crossref","first-page":"271","DOI":"10.3390\/toxins15040271","article-title":"Impact of Enniatin and Deoxynivalenol Co-occurrence on plant, microbial, insect, animal and human systems: current knowledge and future perspectives","volume":"15","author":"Valenti","year":"2023","journal-title":"Toxins"},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib40","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-33070-1","article-title":"FXR agonist obeticholic acid induces liver growth but exacerbates biliary injury in rats with obstructive cholestasis","volume":"8","author":"Van Golen","year":"2018","journal-title":"Sci. Rep."},{"issue":"5","key":"10.1016\/j.ecoenv.2025.118489_bib41","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1007\/s00204-022-03256-8","article-title":"Exposure to the mycotoxin deoxynivalenol reduces the transport of conjugated bile acids by intestinal Caco-2 cells","volume":"96","author":"Wang","year":"2022","journal-title":"Arch. Toxicol."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib42","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jhep.2020.02.007","article-title":"Genotype correlates with the natural history of severe bile salt export pump deficiency","volume":"3","author":"Wessel","year":"2020","journal-title":"J. Hepatol."},{"issue":"3","key":"10.1016\/j.ecoenv.2025.118489_bib43","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1007\/s00204-022-03431-x","article-title":"The circadian rhythm gene Bmal1 ameliorates acute deoxynivalenol-induced liver damage","volume":"97","author":"Yang","year":"2023","journal-title":"Arch. Toxicol."},{"issue":"3","key":"10.1016\/j.ecoenv.2025.118489_bib44","doi-asserted-by":"crossref","first-page":"327","DOI":"10.3390\/ijms17030327","article-title":"Endoplasmic reticulum stress and associated ROS","volume":"17","author":"Zeeshan","year":"2016","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118489_bib45","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/s41392-021-00713-1","article-title":"AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma","volume":"6","author":"Zhu","year":"2021","journal-title":"Signal Transduct. Target Ther."},{"issue":"4","key":"10.1016\/j.ecoenv.2025.118489_bib46","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1097\/HEP.0000000000000611","article-title":"Bile acid\u2013induced IRF3 phosphorylation mediates cell death, inflammatory responses, and fibrosis in cholestasis-induced liver and kidney injury via regulation of ZBP1","volume":"79","author":"Zhuang","year":"2024","journal-title":"Hepatology"},{"key":"10.1016\/j.ecoenv.2025.118489_bib47","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2020.107328","article-title":"An insight into the mechanism and molecular basis of dysfunctional immune response involved in cholestasis","volume":"92","author":"Zou","year":"2021","journal-title":"Int. Immunopharmacol."}],"container-title":["Ecotoxicology and Environmental Safety"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651325008346?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651325008346?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T08:47:15Z","timestamp":1762246035000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147651325008346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":47,"alternative-id":["S0147651325008346"],"URL":"https:\/\/doi.org\/10.1016\/j.ecoenv.2025.118489","relation":{},"ISSN":["0147-6513"],"issn-type":[{"value":"0147-6513","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mechanistic insights into deoxynivalenol-Induced hepatic cholestasis via IRE1\u03b1\/HNF1\u03b1\/FXR signaling dysregulation in mice","name":"articletitle","label":"Article Title"},{"value":"Ecotoxicology and Environmental Safety","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ecoenv.2025.118489","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"118489"}}