{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T17:54:36Z","timestamp":1755798876705,"version":"3.44.0"},"reference-count":89,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009133","name":"Karlsruhe Institute of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009133","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research Bonn Office","doi-asserted-by":"publisher","award":["02WCL1471N"],"award-info":[{"award-number":["02WCL1471N"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ecotoxicology and Environmental Safety"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.ecoenv.2025.118526","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T03:19:33Z","timestamp":1750648773000},"page":"118526","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["S9 metabolism integrated CALUX\u00ae assay reveals invisible endocrine activities of sediments from two eutrophic freshwater lakes in China"],"prefix":"10.1016","volume":"302","author":[{"given":"Feifei","family":"Xue","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1533-798X","authenticated-orcid":false,"given":"Cora","family":"Schmid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunlu","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inska S.","family":"Reichstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9898-5722","authenticated-orcid":false,"given":"Elke","family":"Dopp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"aus der Beek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Tiehm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daqiang","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Schiwy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henner","family":"Hollert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.ecoenv.2025.118526_bib1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0378-4274(85)90046-3","article-title":"Toxicity determined in vitro by morphological alterations and neutral red absorption","volume":"24","author":"Borenfreund","year":"1985","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.ecoenv.2025.118526_bib2","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.scitotenv.2016.10.104","article-title":"Towards the review of the European Union water framework directive: recommendations for more efficient assessment and management of chemical contamination in European surface water resources","volume":"576","author":"Brack","year":"2017","journal-title":"Sci. Total Environ."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118526_bib3","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s12302-019-0192-2","article-title":"Effect-based methods are key. The European Collaborative Project SOLUTIONS recommends integrating effect-based methods for diagnosis and monitoring of water quality","volume":"31","author":"Brack","year":"2019","journal-title":"Environ. Sci. Eur."},{"key":"10.1016\/j.ecoenv.2025.118526_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.142269","article-title":"Using a high-throughput method in the micronucleus assay to compare animal-free with rat-derived S9","volume":"751","author":"Brendt","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.143522","article-title":"Is a liver comparable to a liver? a comparison of different rat-derived S9-fractions with a biotechnological animal-free alternative in the Ames fluctuation assay","volume":"759","author":"Brendt","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib6","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jes.2015.06.008","article-title":"Spatial variations of macrozoobenthos and sediment nutrients in Lake Yangcheng: emphasis on effect of pen culture of Chinese mitten crab","volume":"37","author":"Chen","year":"2015","journal-title":"J. Environ. Sci. (China)"},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118526_bib7","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12302-022-00613-3","article-title":"Commentary: assessing the endocrine disrupting effects of chemicals on invertebrates in the European Union","volume":"34","author":"Crane","year":"2022","journal-title":"Environ. Sci. Eur."},{"key":"10.1016\/j.ecoenv.2025.118526_bib8","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.ecoenv.2017.02.045","article-title":"Distribution and bioaccumulation of endocrine disrupting chemicals in water, sediment and fishes in a shallow Chinese freshwater lake: Implications for ecological and human health risks","volume":"140","author":"Dan","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2025.118526_bib9","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.vascn.2018.03.002","article-title":"mRNA transfection retrofits cell-based assays with xenobiotic metabolism","volume":"92","author":"DeGroot","year":"2018","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"10.1016\/j.ecoenv.2025.118526_bib10","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.scitotenv.2016.07.059","article-title":"Downscaling procedures reduce chemical use in androgen receptor reporter gene assay","volume":"571","author":"Di Paolo","year":"2016","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib11","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.watres.2016.08.018","article-title":"Bioassay battery interlaboratory investigation of emerging contaminants in spiked water extracts \u2013 towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring","volume":"104","author":"Di Paolo","year":"2016","journal-title":"Water Res"},{"key":"10.1016\/j.ecoenv.2025.118526_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.150845","article-title":"Acute and chronic toxicity assessments of 17\u03b2-estradiol (E2) and 17\u03b1-ethinylestradiol (EE2) on the calanoid copepod Acartia clausi: effects on survival, development, sex-ratio and reproduction","volume":"807","author":"Djebbi","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib13","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.watres.2015.05.049","article-title":"Effect-based trigger values for in vitro bioassays: reading across from existing water quality guideline values","volume":"81","author":"Escher","year":"2015","journal-title":"Water Res"},{"key":"10.1016\/j.ecoenv.2025.118526_bib14","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/j.scitotenv.2018.01.340","article-title":"Effect-based trigger values for in vitro and in vivo bioassays performed on surface water extracts supporting the environmental quality standards (EQS) of the European Water framework directive","volume":"628-629","author":"Escher","year":"2018","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2021.131396","article-title":"Occurrence, spatial distribution and risk assessment of high concern endocrine-disrupting chemicals in Jiangsu Province, China","volume":"285","author":"Fan","year":"2021","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2025.118526_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2022.107608","article-title":"Endocrine disrupting chemicals entering European rivers: occurrence and adverse mixture effects in treated wastewater","volume":"170","author":"Finckh","year":"2022","journal-title":"Environ. Int."},{"issue":"21","key":"10.1016\/j.ecoenv.2025.118526_bib17","doi-asserted-by":"crossref","first-page":"13365","DOI":"10.1021\/acs.est.0c04226","article-title":"Endocrine disruption in invertebrates: a survey of research progress","volume":"54","author":"Ford","year":"2020","journal-title":"Environ. Sci. Technol."},{"issue":"4","key":"10.1016\/j.ecoenv.2025.118526_bib18","doi-asserted-by":"crossref","DOI":"10.1065\/jss2007.07.240","article-title":"Environmental quality standards (EQS) applicable to sediment and\/or biota","volume":"7","author":"F\u00f6rstner","year":"2007","journal-title":"J. Soils Sediment."},{"key":"10.1016\/j.ecoenv.2025.118526_bib19","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.ecoenv.2019.01.001","article-title":"Exposure and ecological risk of phthalate esters in the Taihu Lake basin, China","volume":"171","author":"Gao","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"issue":"5-12","key":"10.1016\/j.ecoenv.2025.118526_bib20","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.marpolbul.2011.01.026","article-title":"Occurrence of endocrine-disrupting chemicals in riverine sediments from the Pearl River Delta, China","volume":"63","author":"Gong","year":"2011","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2025.118526_bib21","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.scitotenv.2014.06.037","article-title":"Occurrence and spatial distribution of EDCs and related compounds in waters and sediments of Iberian rivers","volume":"503-504","author":"Gorga","year":"2015","journal-title":"Sci. Total Environ."},{"issue":"3","key":"10.1016\/j.ecoenv.2025.118526_bib22","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1007\/s11356-010-0390-3","article-title":"The endocrine disrupting potential of sediments from the Upper Danube River (Germany) as revealed by in vitro bioassays and chemical analysis","volume":"18","author":"Grund","year":"2011","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.ecoenv.2025.118526_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2024.135920","article-title":"Do pyrethroid pesticide residues in Chinese mitten crab aquaculture areas have an impact on the ecological environment? \u2014\u2014a case study of Yangcheng Lake","volume":"480","author":"Gu","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.ecoenv.2025.118526_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2021.112136","article-title":"Combined toxicity of endocrine-disrupting chemicals: a review","volume":"215","author":"Hamid","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2025.118526_bib25","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.jes.2022.09.013","article-title":"Polycyclic aromatic hydrocarbons (PAHs) in a sediment core from Lake Taihu and their associations with sedimentary organic matter","volume":"129","author":"He","year":"2023","journal-title":"J. Environ. Sci."},{"key":"10.1016\/j.ecoenv.2025.118526_bib26","doi-asserted-by":"crossref","DOI":"10.3389\/ftox.2023.1196245","article-title":"Development of a bioprinter-based method for incorporating metabolic competence into high-throughput in vitro assays","volume":"5","author":"Hopperstad","year":"2023","journal-title":"Front. Toxicol."},{"issue":"20","key":"10.1016\/j.ecoenv.2025.118526_bib27","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1016\/j.jchromb.2011.04.020","article-title":"Quantitative analysis of estrogens and estrogen metabolites in endogenous MCF-7 breast cancer cells by liquid chromatography-tandem mass spectrometry","volume":"879","author":"Huang","year":"2011","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.ecoenv.2025.118526_bib28","unstructured":"ISO 19040-3, 2018. Water quality \u2014 Determination of the estrogenic potential of water and waste water\u2014Part 3: In vitro human cell-based reporter gene assay. \u3008https:\/\/www.iso.org\/standard\/66297.html\u3009."},{"issue":"Pt 1","key":"10.1016\/j.ecoenv.2025.118526_bib29","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1016\/j.scitotenv.2018.09.199","article-title":"Effect-based and chemical analyses of agonistic and antagonistic endocrine disruptors in multiple matrices of eutrophic freshwaters","volume":"651","author":"Jia","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib30","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.envpol.2018.11.021","article-title":"Cyanobacterial blooms act as sink and source of endocrine disruptors in the third largest freshwater lake in China","volume":"245","author":"Jia","year":"2019","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.ecoenv.2025.118526_bib31","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.scitotenv.2019.02.435","article-title":"Toxicological and ecotoxicological evaluation of the water quality in a large and eutrophic freshwater lake of China","volume":"667","author":"Jia","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib32","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.watres.2016.07.059","article-title":"Occurrence and partitioning of bisphenol analogues in water and sediment from Liaohe River Basin and Taihu Lake, China","volume":"103","author":"Jin","year":"2016","journal-title":"Water Res"},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118526_bib33","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1289\/ehp.8046","article-title":"Introduction: the ecological relevance of chemically induced endocrine disruption in wildlife","volume":"114","author":"Jobling","year":"2006","journal-title":"Environ. Health Perspect."},{"issue":"7","key":"10.1016\/j.ecoenv.2025.118526_bib34","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1124\/dmd.108.020370","article-title":"Bioactivation of flutamide metabolites by human liver microsomes","volume":"36","author":"Kang","year":"2008","journal-title":"Drug Metab. Dispos."},{"issue":"5","key":"10.1016\/j.ecoenv.2025.118526_bib35","doi-asserted-by":"crossref","first-page":"4051","DOI":"10.1007\/s11356-017-0155-3","article-title":"A hierarchical testing strategy for micropollutants in drinking water regarding their potential endocrine-disrupting effects\u2014towards health-related indicator values","volume":"25","author":"Kuckelkorn","year":"2018","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.ecoenv.2025.118526_bib36","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.watres.2016.10.062","article-title":"Effect-based tools for monitoring estrogenic mixtures: evaluation of five in vitro bioassays","volume":"110","author":"Kunz","year":"2017","journal-title":"Water Res"},{"key":"10.1016\/j.ecoenv.2025.118526_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2023.119692","article-title":"Effect-based monitoring of two rivers under urban and agricultural influence reveals a range of biological activities in sediment and water extracts","volume":"351","author":"Leusch","year":"2024","journal-title":"J. Environ. Manag."},{"key":"10.1016\/j.ecoenv.2025.118526_bib38","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.scitotenv.2016.11.097","article-title":"National investigation of semi-volatile organic compounds (PAHs, OCPs, and PCBs) in lake sediments of China: occurrence, spatial variation and risk assessment","volume":"579","author":"Li","year":"2017","journal-title":"Sci. Total Environ."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118526_bib39","first-page":"155","article-title":"Fluctuations of nutrient concentrations and their influencing factors under various net-pen aquaculture scales in East Lake Taihu from 1990 to 2021","volume":"35","author":"Li","year":"2023","journal-title":"J. Lake Sci."},{"issue":"1-2","key":"10.1016\/j.ecoenv.2025.118526_bib40","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.marpolbul.2016.08.026","article-title":"Occurrence, distribution, and risk assessment of alkylphenols, bisphenol A, and tetrabromobisphenol A in surface water, suspended particulate matter, and sediment in Taihu Lake and its tributaries","volume":"112","author":"Liu","year":"2016","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.ecoenv.2025.118526_bib41","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.ecoenv.2017.02.045","article-title":"Distribution and bioaccumulation of endocrine disrupting chemicals in water, sediment and fishes in a shallow Chinese freshwater lake: implications for ecological and human health risks","volume":"140","author":"Liu","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2025.118526_bib42","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.envpol.2017.05.031","article-title":"Steroid bioaccumulation profiles in typical freshwater aquaculture environments of South China and their human health risks via fish consumption","volume":"228","author":"Liu","year":"2017","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.ecoenv.2025.118526_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2020.111064","article-title":"Impact of rainfall on the occurrence, spatiotemporal distribution, and partition trend of micropollutants in Taihu Lake, China: bisphenol A and 4-nonylphenol as examples","volume":"204","author":"Liu","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2025.118526_bib44","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2024.120188","article-title":"Occurrence, bioaccumulation, and partitioning of phthalate acid esters in the third largest freshwater lake (Lake Taihu) in China","volume":"263","author":"Liu","year":"2024","journal-title":"Environ. Res."},{"issue":"3","key":"10.1016\/j.ecoenv.2025.118526_bib45","doi-asserted-by":"crossref","first-page":"389","DOI":"10.14573\/altex.1611021","article-title":"Incorporation of a metabolizing system in biodetection assays for endocrine active substances","volume":"34","author":"Mollergues","year":"2017","journal-title":"ALTEX"},{"key":"10.1016\/j.ecoenv.2025.118526_bib46","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.watres.2019.06.020","article-title":"Bioavailability of estrogenic compounds from sediment in the context of flood events evaluated by passive sampling","volume":"161","author":"M\u00fcller","year":"2019","journal-title":"Water Res"},{"issue":"17","key":"10.1016\/j.ecoenv.2025.118526_bib47","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1289\/ehp.7209","article-title":"Long-term exposure to environmental concentrations of the pharmaceutical ethynylestradiol causes reproductive failure in fish","volume":"112","author":"Nash","year":"2004","journal-title":"Environ. Health Perspect."},{"year":"2014","series-title":"Detailed Review Paper on the Use of Metabolising Systems for In Vitro Testing of Endocrine Disruptors, OECD Series on Testing and Assessment","author":"OECD","key":"10.1016\/j.ecoenv.2025.118526_bib48"},{"year":"2018","series-title":"Guidance Document on Good In Vitro Method Practices (GIVIMP), OECD Series on Testing and Assessment","author":"OECD","key":"10.1016\/j.ecoenv.2025.118526_bib49"},{"key":"10.1016\/j.ecoenv.2025.118526_bib50","series-title":"OECD Guidelines for the Testing of Chemicals, Section 4","article-title":"Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists","author":"OECD TG 455","year":"2021"},{"year":"2023","series-title":"Stably Transfected Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals, OECD Guidelines for the Testing of Chemicals, Section 4","author":"OECD TG 458","key":"10.1016\/j.ecoenv.2025.118526_bib51"},{"issue":"4","key":"10.1016\/j.ecoenv.2025.118526_bib52","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.ijheh.2019.04.008","article-title":"Evaluation of mixture effects of endocrine active substances in wastewater using CALUX reporter-gene assays","volume":"222","author":"Pannekens","year":"2019","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"10.1016\/j.ecoenv.2025.118526_bib53","series-title":"Casarett and Doull's Toxicology: The Basic Science of Poisons","article-title":"Biotransformation of xenobiotics","author":"Parkinson","year":"2013"},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118526_bib54","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s10750-006-0521-5","article-title":"Environmental issues of Lake Taihu, China","volume":"581","author":"Qin","year":"2007","journal-title":"Hydrobiologia"},{"issue":"3S","key":"10.1016\/j.ecoenv.2025.118526_bib55","doi-asserted-by":"crossref","first-page":"05","DOI":"10.33545\/26174693.2024.v8.i3Sa.677","article-title":"Endocrine disrupting chemicals and their harmful effects in fish: a comprehensive review","volume":"8","author":"Ramasre","year":"2024","journal-title":"Int. J. Adv. Biochem. Res."},{"key":"10.1016\/j.ecoenv.2025.118526_bib56","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.161454","article-title":"Replacing animal-derived components in in vitro test guidelines OECD 455 and 487","volume":"868","author":"Reichstein","year":"2023","journal-title":"Sci. Total Environ."},{"issue":"4","key":"10.1016\/j.ecoenv.2025.118526_bib57","doi-asserted-by":"crossref","first-page":"500","DOI":"10.2174\/0929867321666140916123022","article-title":"Biotransformation of endocrine disrupting compounds by selected phase I and phase II enzymes \u2013 formation of estrogenic and chemically reactive metabolites by cytochromes P450 and sulfotransferases","volume":"22","author":"Reinen","year":"2015","journal-title":"Curr. Med. Chem."},{"issue":"7","key":"10.1016\/j.ecoenv.2025.118526_bib58","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1038\/nprot.2008.75","article-title":"Neutral red uptake assay for the estimation of cell viability\/cytotoxicity","volume":"3","author":"Repetto","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ecoenv.2025.118526_bib59","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.scitotenv.2015.05.048","article-title":"Occurrence, distribution and bioaccumulation of endocrine disrupting compounds in water, sediment and biota samples from a European river basin","volume":"529","author":"Salgueiro-Gonzalez","year":"2015","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib60","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jhazmat.2015.05.046","article-title":"The effect of particle size on sorption of estrogens, androgens and progestagens in aquatic sediment","volume":"299","author":"Sangster","year":"2015","journal-title":"J. Hazard. Mater."},{"issue":"8","key":"10.1016\/j.ecoenv.2025.118526_bib61","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1007\/s11356-012-0852-x","article-title":"Effect directed analysis and mixture effects of estrogenic compounds in a sediment of the river Elbe","volume":"19","author":"Schmitt","year":"2012","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.ecoenv.2025.118526_bib62","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.15192","article-title":"Chemical and toxicological assessment of leachates from UV-degraded plastic materials using in-vitro bioassays","volume":"11","author":"Schwarz","year":"2023","journal-title":"PeerJ"},{"key":"10.1016\/j.ecoenv.2025.118526_bib63","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.141468","article-title":"Optimization of a pre-metabolization procedure using rat liver S9 and cell-extracted S9 in the Ames fluctuation test","volume":"749","author":"Shao","year":"2020","journal-title":"Sci. Total Environ."},{"issue":"11","key":"10.1016\/j.ecoenv.2025.118526_bib64","first-page":"1298","article-title":"Metabolism of the antiandrogenic drug (Flutamide) by human CYP1A2","volume":"25","author":"Shet","year":"1997","journal-title":"Drug Metab. Dispos."},{"issue":"Pt 2","key":"10.1016\/j.ecoenv.2025.118526_bib65","article-title":"Extensive rain events have a more substantial impact than advanced effluent treatment on the endocrine-disrupting activity in an effluent-dominated small river","volume":"807","author":"Shuliakevich","year":"2022","journal-title":"Sci. Total Environ."},{"issue":"8","key":"10.1016\/j.ecoenv.2025.118526_bib66","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1021\/es0101227","article-title":"Something from \u201cnothing\u201d \u2212 eight weak estrogenic chemicals combined at concentrations below NOECs produce significant mixture effects","volume":"36","author":"Silva","year":"2002","journal-title":"Environ. Sci. Technol."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118526_bib67","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1093\/toxsci\/kfi005","article-title":"Development of androgen- and estrogen-responsive bioassays, members of a panel of human cell line-based highly selective steroid-responsive bioassays","volume":"83","author":"Sonneveld","year":"2004","journal-title":"Toxicol. Sci."},{"issue":"2","key":"10.1016\/j.ecoenv.2025.118526_bib68","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1007\/s11368-019-02429-w","article-title":"Sedimentary records of historical occurrence and sources of 21 organochlorine pesticides in Lake Taihu, China","volume":"20","author":"Tao","year":"2019","journal-title":"J. Soils Sediment."},{"issue":"11-12","key":"10.1016\/j.ecoenv.2025.118526_bib69","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1351\/pac200375111917","article-title":"Role of metabolism in the endocrine-disrupting effects of chemicals in aquatic and terrestrial systems","volume":"75","author":"van den Berg","year":"2003","journal-title":"Pure Appl. Chem."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118526_bib70","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.reprotox.2010.04.007","article-title":"Optimization and prevalidation of the in vitro ER\u03b1 CALUX method to test estrogenic and antiestrogenic activity of compounds","volume":"30","author":"van der Burg","year":"2010","journal-title":"Reprod. Toxicol."},{"issue":"15","key":"10.1016\/j.ecoenv.2025.118526_bib71","doi-asserted-by":"crossref","first-page":"5814","DOI":"10.1021\/es702897y","article-title":"Detection of multiple hormonal activities in wastewater effluents and surface water, using a panel of steroid receptor CALUX bioassays","volume":"42","author":"van der Linden","year":"2008","journal-title":"Environ. Sci. Technol."},{"issue":"9","key":"10.1016\/j.ecoenv.2025.118526_bib72","doi-asserted-by":"crossref","first-page":"2385","DOI":"10.1002\/etc.3836","article-title":"SIMONI (smart integrated monitoring) as a novel bioanalytical strategy for water quality assessment: Part i-model design and effect-based trigger values","volume":"36","author":"van der Oost","year":"2017","journal-title":"Environ. Toxicol. Chem."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118526_bib73","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.etap.2004.03.010","article-title":"Formation of estrogenic metabolites of benzo[a]pyrene and chrysene by cytochrome P450 activity and their combined and supra-maximal estrogenic activity","volume":"19","author":"van Lipzig","year":"2005","journal-title":"Environ. Toxicol. Pharm."},{"key":"10.1016\/j.ecoenv.2025.118526_bib74","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.reprotox.2017.11.005","article-title":"Incorporation of metabolic enzymes to improve predictivity of reporter gene assay results for estrogenic and anti-androgenic activity","volume":"75","author":"van Vugt-Lussenburg","year":"2018","journal-title":"Reprod. Toxicol."},{"issue":"1","key":"10.1016\/j.ecoenv.2025.118526_bib75","doi-asserted-by":"crossref","DOI":"10.1016\/j.jece.2020.104558","article-title":"Endocrine-disrupting compounds: Occurrence, detection methods, effects and promising treatment pathways\u2014a critical review","volume":"9","author":"Vieira","year":"2021","journal-title":"J. Environ. Chem. Eng."},{"key":"10.1016\/j.ecoenv.2025.118526_bib76","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.aquatox.2015.10.022","article-title":"Exposing native cyprinid (Barbus plebejus) juveniles to river sediments leads to gonadal alterations, genotoxic effects and thyroid disruption","volume":"169","author":"Vigan\u00f2","year":"2015","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.ecoenv.2025.118526_bib77","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2020.106191","article-title":"Receptor-based in vitro activities to assess human exposure to chemical mixtures and related health impacts","volume":"146","author":"Vinggaard","year":"2021","journal-title":"Environ. Int."},{"key":"10.1016\/j.ecoenv.2025.118526_bib78","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.ecolind.2017.06.038","article-title":"Ecological and health risk assessment of PAHs, OCPs, and PCBs in Taihu Lake basin","volume":"92","author":"Wang","year":"2018","journal-title":"Ecol. Indic."},{"key":"10.1016\/j.ecoenv.2025.118526_bib79","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.146826","article-title":"Occurrence, spatiotemporal distribution, and risk assessment of current-use pesticides in surface water: a case study near Taihu Lake, China","volume":"782","author":"Wang","year":"2021","journal-title":"Sci. Total Environ."},{"issue":"5","key":"10.1016\/j.ecoenv.2025.118526_bib80","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1007\/s00216-009-2807-8","article-title":"Masking effect of anti-androgens on androgenic activity in European river sediment unveiled by effect-directed analysis","volume":"394","author":"Weiss","year":"2009","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.ecoenv.2025.118526_bib81","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.149756","article-title":"Improvement of wastewater and water quality via a full-scale ozonation plant? - A comprehensive analysis of the endocrine potential using effect-based methods","volume":"803","author":"Wolf","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib82","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.chemosphere.2017.06.010","article-title":"Bisphenol analogues in surface water and sediment from the shallow Chinese freshwater lakes: occurrence, distribution, source apportionment, and ecological and human health risk","volume":"184","author":"Yan","year":"2017","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2025.118526_bib83","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.169821","article-title":"Thirty years of experience in water pollution control in Taihu Lake: a review","volume":"914","author":"Yan","year":"2024","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.118526_bib84","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2021.114380","article-title":"Pollution gradients shape the co-occurrence networks and interactions of sedimentary bacterial communities in Taihu Lake, a shallow eutrophic lake","volume":"305","author":"Zhang","year":"2022","journal-title":"J. Environ. Manag."},{"issue":"9","key":"10.1016\/j.ecoenv.2025.118526_bib85","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/j.chemosphere.2009.09.022","article-title":"Distribution and bioaccumulation of organochlorine pesticides in surface sediments and benthic organisms from Taihu Lake, China","volume":"77","author":"Zhao","year":"2009","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2025.118526_bib86","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.ecoenv.2017.03.039","article-title":"Spatial correlation analysis of polycyclic aromatic hydrocarbons (PAHs) and organochlorine pesticides (OCPs) in sediments between Taihu Lake and its tributary rivers","volume":"142","author":"Zhao","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2025.118526_bib87","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2024.141598","article-title":"Assessment of occurrence, partitioning and ecological risk for 144 steroid hormones in Taihu Lake using UPLC-MS\/MS with machine learning model","volume":"354","author":"Zhao","year":"2024","journal-title":"Chemosphere"},{"key":"10.1016\/j.ecoenv.2025.118526_bib88","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.scitotenv.2016.03.059","article-title":"Occurrence, spatiotemporal distribution, and ecological risks of steroids in a large shallow Chinese lake, Lake Taihu","volume":"557-558","author":"Zhou","year":"2016","journal-title":"Sci. Total Environ."},{"issue":"4","key":"10.1016\/j.ecoenv.2025.118526_bib89","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.steroids.2005.01.002","article-title":"NADPH-dependent metabolism of 17beta-estradiol and estrone to polar and nonpolar metabolites by human tissues and cytochrome P450 isoforms","volume":"70","author":"Zhu","year":"2005","journal-title":"Steroids"}],"container-title":["Ecotoxicology and Environmental Safety"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651325008711?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651325008711?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T14:49:54Z","timestamp":1755614994000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147651325008711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":89,"alternative-id":["S0147651325008711"],"URL":"https:\/\/doi.org\/10.1016\/j.ecoenv.2025.118526","relation":{},"ISSN":["0147-6513"],"issn-type":[{"type":"print","value":"0147-6513"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"S9 metabolism integrated CALUX\u00ae assay reveals invisible endocrine activities of sediments from two eutrophic freshwater lakes in China","name":"articletitle","label":"Article Title"},{"value":"Ecotoxicology and Environmental Safety","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ecoenv.2025.118526","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"118526"}}