{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:16:21Z","timestamp":1790997381680,"version":"4.1.0"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018627","name":"Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018627","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010029","name":"Taishan Scholar Foundation of Shandong Province","doi-asserted-by":"publisher","award":["tsqn202211359"],"award-info":[{"award-number":["tsqn202211359"]}],"id":[{"id":"10.13039\/501100010029","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ecotoxicology and Environmental Safety"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.ecoenv.2025.119089","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T01:48:07Z","timestamp":1758764887000},"page":"119089","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Single-cell RNA sequencing across diverse cell types to identify PFOA-induced ovarian cellular senescence"],"prefix":"10.1016","volume":"304","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4186-7706","authenticated-orcid":false,"given":"Ning","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahao","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ecoenv.2025.119089_bib1","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1080\/15376516.2024.2335966","article-title":"Assessment of TGx-DDI genes for genotoxicity in a comprehensive panel of chemicals","volume":"34","author":"Barutcu","year":"2024","journal-title":"Toxicol. Mech. Methods"},{"key":"10.1016\/j.ecoenv.2025.119089_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2021.106589","article-title":"Persistent organic pollutants and the size of ovarian reserve in reproductive-aged women","volume":"155","author":"Bj\u00f6rvang","year":"2021","journal-title":"Environ. Int."},{"key":"10.1016\/j.ecoenv.2025.119089_bib3","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1093\/biolre\/ioae048","article-title":"Quantification of follicles in human ovarian tissue using image processing software and trained artificial intelligence","volume":"110","author":"Blevins","year":"2024","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ecoenv.2025.119089_bib4","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.reprotox.2022.03.011","article-title":"Perfluorooctanoic acid promotes proliferation of the human granulosa cell line HGrC1 and alters expression of cell cycle genes and hippo pathway effector YAP1","volume":"110","author":"Clark","year":"2022","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.ecoenv.2025.119089_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2024.108850","article-title":"Quantifying levels of per- and polyfluoroalkyl substances (PFAS) in water and serum after contamination from agricultural biosolid application","volume":"190","author":"Criswell","year":"2024","journal-title":"Environ. Int."},{"key":"10.1016\/j.ecoenv.2025.119089_bib6","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/s41422-022-00718-7","article-title":"Unraveling female reproductive senescence to enhance healthy longevity","volume":"33","author":"Dong","year":"2023","journal-title":"Cell Res"},{"key":"10.1016\/j.ecoenv.2025.119089_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2025.117816","article-title":"Prenatal perfluorooctanoic acid (PFOA) exposure causes reproductive toxicity by disrupting the formation of transzonal projections (TZPs) and down-regulating Wnt4\/\u03b2-catenin signaling pathway in progeny","volume":"291","author":"Du","year":"2025","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2025.119089_bib8","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2024.1396192","article-title":"Insulin-like growth factor family and prostate cancer: new insights and emerging opportunities","volume":"15","author":"Elemam","year":"2024","journal-title":"Front. Endocrinol."},{"issue":"4","key":"10.1016\/j.ecoenv.2025.119089_bib9","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1007\/s43032-021-00691-3","article-title":"The differential metabolomes in cumulus and mural granulosa cells from human preovulatory follicles","volume":"29","author":"Gao","year":"2022","journal-title":"Reprod. Sci."},{"key":"10.1016\/j.ecoenv.2025.119089_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2024.108703","article-title":"Sub-acute exposure of Male guppies (poecilia reticulata) to environmentally relevant concentrations of PFOA and GenX induces significant changes in the testis transcriptome and reproductive traits","volume":"187","author":"Gasparini","year":"2024","journal-title":"Environ. Int."},{"key":"10.1016\/j.ecoenv.2025.119089_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2021.106000","article-title":"Elucidation of the anti-inflammatory mechanism of er miao san by integrative approach of network pharmacology and experimental verification","volume":"175","author":"Guo","year":"2022","journal-title":"Pharm. Res"},{"key":"10.1016\/j.ecoenv.2025.119089_bib12","doi-asserted-by":"crossref","DOI":"10.1093\/humrep\/deae108.711","article-title":"P-353 perfluorooctanoic acid (PFOA) impacts murine ovarian follicle development through apoptotic signaling pathways and exhibits transgenerational effects","volume":"39","author":"Guo","year":"2024","journal-title":"Hum. Reprod."},{"issue":"5","key":"10.1016\/j.ecoenv.2025.119089_bib13","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1111\/jog.15918","article-title":"Cellular senescence in the pathogenesis of ovarian dysfunction","volume":"50","author":"Harada","year":"2024","journal-title":"J. Obstet. Gynaecol. Res"},{"key":"10.1016\/j.ecoenv.2025.119089_bib14","doi-asserted-by":"crossref","DOI":"10.1530\/REP-23-0499","article-title":"Reproductive ageing: inflammation, immune cells, and cellular senescence in the aging ovary","volume":"168","author":"Isola","year":"2024","journal-title":"Reproduction"},{"key":"10.1016\/j.ecoenv.2025.119089_bib15","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2023.1124405","article-title":"Roles of endoplasmic reticulum stress in the pathophysiology of polycystic ovary syndrome","volume":"14","author":"Koike","year":"2023","journal-title":"Front Endocrinol. (Lausanne)"},{"key":"10.1016\/j.ecoenv.2025.119089_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2024.175609","article-title":"Adolescent F-53B exposure induces ovarian toxicity in rats: Autophagy-apoptosis interplay","volume":"951","author":"Lan","year":"2024","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.119089_bib17","doi-asserted-by":"crossref","first-page":"2699","DOI":"10.2147\/JIR.S496130","article-title":"Single-Cell RNA sequencing reveals peripheral immune cell senescence and inflammatory phenotypes in patients with premature ovarian failure","volume":"18","author":"Liu","year":"2025","journal-title":"J. Inflamm. Res"},{"issue":"1","key":"10.1016\/j.ecoenv.2025.119089_bib18","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1002\/tox.22661","article-title":"Perfluorooctanoic acid disrupts gap junction intercellular communication and induces reactive oxygen species formation and apoptosis in mouse ovaries","volume":"34","author":"L\u00f3pez-Arellano","year":"2019","journal-title":"Environ. Toxicol."},{"issue":"19","key":"10.1016\/j.ecoenv.2025.119089_bib19","doi-asserted-by":"crossref","first-page":"8160","DOI":"10.1021\/es1038694","article-title":"Association of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) with age of puberty among children living near a chemical plant","volume":"45","author":"Lopez-Espinosa","year":"2011","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.ecoenv.2025.119089_bib20","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1177\/0192623308317428","article-title":"Ovarian follicle counts using proliferating cell nuclear antigen (PCNA) and Semi-Automated image analysis in rats","volume":"36","author":"Picut","year":"2008","journal-title":"Toxicol. Pathol."},{"issue":"2","key":"10.1016\/j.ecoenv.2025.119089_bib21","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1093\/humupd\/dmab038","article-title":"The role of cellular senescence in female reproductive aging and the potential for senotherapeutic interventions","volume":"28","author":"Secomandi","year":"2022","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.ecoenv.2025.119089_bib22","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s12958-023-01056-y","article-title":"Effect of PFOA exposure on diminished ovarian reserve and its metabolism","volume":"21","author":"Shen","year":"2023","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.ecoenv.2025.119089_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2025.137375","article-title":"Insight into perfluorooctanoic acid-induced impairment of mouse embryo implantation via single-cell RNA-seq","volume":"488","author":"Sheng","year":"2025","journal-title":"J. Hazard Mater."},{"key":"10.1016\/j.ecoenv.2025.119089_bib24","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2024.1347286","article-title":"Effects of reactive oxygen species and mitochondrial dysfunction on reproductive aging","volume":"12","author":"Song","year":"2024","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.ecoenv.2025.119089_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.tox.2025.154083","article-title":"Mitochondrial activity and steroid secretion in mouse ovarian granulosa cells are suppressed by a PFAS mixture","volume":"512","author":"Tatarczuch","year":"2025","journal-title":"Toxicology"},{"key":"10.1016\/j.ecoenv.2025.119089_bib26","doi-asserted-by":"crossref","DOI":"10.4028\/b-wjQRP6","article-title":"Sleep deprivation triggers the excessive activation of ovarian primordial follicles via \u03b22 adrenergic receptor signaling","volume":"11","author":"Weng","year":"2024","journal-title":"Adv. Sci."},{"key":"10.1016\/j.ecoenv.2025.119089_bib27","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1038\/s41580-024-00775-3","article-title":"Roles of chromatin and genome instability in cellular senescence and their relevance to ageing and related diseases","volume":"25","author":"Wu","year":"2024","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.ecoenv.2025.119089_bib28","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1093\/toxsci\/kfac005","article-title":"Perfluorooctanoic acid disrupts ovarian steroidogenesis and folliculogenesis in adult mice","volume":"186","author":"Yang","year":"2022","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.ecoenv.2025.119089_bib29","article-title":"PM2.5 exposure reprograms cell cycle dynamics in uterine immune cells at single-cell resolution","volume":"16","author":"Zhang","year":"2025","journal-title":"Front Immunol."},{"key":"10.1016\/j.ecoenv.2025.119089_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.166954","article-title":"Perfluorooctanoic acid exposure leads to defect in follicular development through disrupting the mitochondrial electron transport chain in granulosa cells","volume":"905","author":"Zhang","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ecoenv.2025.119089_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2024.116907","article-title":"Environmentally relevant concentration PFNA promotes degradation of SMAD7 to drive progression of ovarian cancer via TGF-\u03b2\/SMADs signaling pathway","volume":"284","author":"Zhong","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2025.119089_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2024.117374","article-title":"Potential causal associations between perfluoroalkyl substances exposure and adverse pregnancy outcomes: a bidirectional two-sample mendelian randomization study","volume":"288","author":"Zhou","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.ecoenv.2025.119089_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2022.107413","article-title":"Perfluorooctanoic acid (PFOA) exposure affects early embryonic development and offspring oocyte quality via inducing mitochondrial dysfunction","volume":"167","author":"Zhou","year":"2022","journal-title":"Environ. Int."}],"container-title":["Ecotoxicology and Environmental Safety"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651325014344?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147651325014344?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:09:43Z","timestamp":1777489783000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147651325014344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":33,"alternative-id":["S0147651325014344"],"URL":"https:\/\/doi.org\/10.1016\/j.ecoenv.2025.119089","relation":{},"ISSN":["0147-6513"],"issn-type":[{"value":"0147-6513","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-cell RNA sequencing across diverse cell types to identify PFOA-induced ovarian cellular senescence","name":"articletitle","label":"Article Title"},{"value":"Ecotoxicology and Environmental Safety","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ecoenv.2025.119089","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"119089"}}