{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:19:39Z","timestamp":1792700379643,"version":"4.5.0"},"reference-count":50,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Cell Biology"],"published-print":{"date-parts":[[2017,8]]},"DOI":"10.1016\/j.ejcb.2017.02.002","type":"journal-article","created":{"date-parts":[[2017,3,7]],"date-time":"2017-03-07T06:45:15Z","timestamp":1488869115000},"page":"440-456","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"title":["Interdependence of thyroglobulin processing and thyroid hormone export in the mouse thyroid gland"],"prefix":"10.1016","volume":"96","author":[{"given":"Jonas","family":"Weber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"McInnes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cise","family":"Kizilirmak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maren","family":"Rehders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Qatato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva K.","family":"Wirth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Schweizer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Verrey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Heuer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaudia","family":"Brix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"12","key":"10.1016\/j.ejcb.2017.02.002_bib0005","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1515\/hsz-2012-0204","article-title":"Effects of cathepsin K deficiency on intercellular junction proteins, luminal mucus layers, and extracellular matrix constituents in the mouse colon","volume":"393","author":"Arampatzidou","year":"2012","journal-title":"Biol. Chem."},{"issue":"5","key":"10.1016\/j.ejcb.2017.02.002_bib0010","first-page":"1918","article-title":"Multimerization of thyroglobulin (TG) during extracellular storage: isolation of highly cross-linked TG from human thyroids","volume":"81","author":"Berndorfer","year":"1996","journal-title":"J. Clin. Endocrinol. Metab."},{"issue":"5","key":"10.1016\/j.ejcb.2017.02.002_bib0015","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1210\/endo.137.5.8612537","article-title":"Evidence for extracellularly acting cathepsins mediating thyroid hormone liberation in thyroid epithelial cells","volume":"137","author":"Brix","year":"1996","journal-title":"Endocrinology"},{"issue":"2","key":"10.1016\/j.ejcb.2017.02.002_bib0020","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1172\/JCI1614","article-title":"Extracellularly occurring histone H1 mediates the binding of thyroglobulin to the cell surface of mouse macrophages","volume":"102","author":"Brix","year":"1998","journal-title":"J. Clin. Invest."},{"issue":"5","key":"10.1016\/j.ejcb.2017.02.002_bib0025","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1515\/bchm.2001.382.5.717","article-title":"Cysteine proteinases mediate extracellular prohormone processing in the thyroid","volume":"382","author":"Brix","year":"2001","journal-title":"Biol. Chem."},{"issue":"2","key":"10.1016\/j.ejcb.2017.02.002_bib0030","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.biochi.2007.07.024","article-title":"Cysteine cathepsins: cellular roadmap to different functions","volume":"90","author":"Brix","year":"2008","journal-title":"Biochimie"},{"issue":"Suppl. 1","key":"10.1016\/j.ejcb.2017.02.002_bib0035","doi-asserted-by":"crossref","first-page":"S9","DOI":"10.1186\/1756-6614-4-S1-S9","article-title":"Molecules important for thyroid hormone synthesis and action\u2013known facts and future perspectives","volume":"4","author":"Brix","year":"2011","journal-title":"Thyroid Res."},{"issue":"3","key":"10.1016\/j.ejcb.2017.02.002_bib0040","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1007\/s00709-014-0730-0","article-title":"Proteolysis mediated by cysteine cathepsins and legumain-recent advances and cell biological challenges","volume":"252","author":"Brix","year":"2015","journal-title":"Protoplasma"},{"issue":"2","key":"10.1016\/j.ejcb.2017.02.002_bib0045","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1007\/s12018-011-9093-7","article-title":"Thyroid cathepsin K: roles in physiology and thyroid disease","volume":"9","author":"Dauth","year":"2011","journal-title":"Clin. Rev. Bone Miner. Metab."},{"issue":"9","key":"10.1016\/j.ejcb.2017.02.002_bib0050","doi-asserted-by":"crossref","first-page":"3377","DOI":"10.1172\/JCI42113","article-title":"Mice deficient in MCT8 reveal a mechanism regulating thyroid hormone secretion","volume":"120","author":"Di Cosmo","year":"2010","journal-title":"J. Clin. Invest."},{"issue":"1","key":"10.1016\/j.ejcb.2017.02.002_bib0055","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1210\/er.2015-1090","article-title":"Thyroglobulin from molecular and cellular biology to clinical endocrinology","volume":"37","author":"Di Jeso","year":"2016","journal-title":"Endocr. Rev."},{"issue":"9","key":"10.1016\/j.ejcb.2017.02.002_bib0060","doi-asserted-by":"crossref","first-page":"4036","DOI":"10.1210\/en.2006-0390","article-title":"Tissue-specific thyroid hormone deprivation and excess in monocarboxylate transporter (mct) 8-deficient mice","volume":"147","author":"Dumitrescu","year":"2006","journal-title":"Endocrinology"},{"issue":"30","key":"10.1016\/j.ejcb.2017.02.002_bib0065","doi-asserted-by":"crossref","first-page":"20198","DOI":"10.1016\/S0021-9258(18)54909-7","article-title":"Thyroglobulin processing by thyroidal proteases Major sites of cleavage by cathepsins B, D, and L","volume":"266","author":"Dunn","year":"1991","journal-title":"J. Biol. Chem."},{"issue":"8","key":"10.1016\/j.ejcb.2017.02.002_bib0070","doi-asserted-by":"crossref","first-page":"3279","DOI":"10.1210\/endo.137.8.8754751","article-title":"The combined action of two thyroidal proteases releases T4 from the dominant hormone-forming site of thyroglobulin","volume":"137","author":"Dunn","year":"1996","journal-title":"Endocrinology"},{"issue":"Suppl. 1","key":"10.1016\/j.ejcb.2017.02.002_bib0075","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000431318","article-title":"Understanding the healthy thyroid state in 2015","volume":"4","author":"F\u00fchrer","year":"2015","journal-title":"Eur. Thyroid J."},{"issue":"5","key":"10.1016\/j.ejcb.2017.02.002_bib0080","doi-asserted-by":"crossref","first-page":"R197","DOI":"10.1530\/EJE-14-0285","article-title":"Beyond the fixed setpoint of the hypothalamus-pituitary-thyroid axis","volume":"171","author":"Fliers","year":"2014","journal-title":"Eur. J. Endocrinol.\/Eur. Feder. Endocr. Soc."},{"issue":"11","key":"10.1016\/j.ejcb.2017.02.002_bib0085","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1172\/JCI15990","article-title":"Thyroid functions of mouse cathepsins B, K, and L","volume":"111","author":"Friedrichs","year":"2003","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.ejcb.2017.02.002_bib0090","first-page":"145","article-title":"Functional morphology of the thyroid","volume":"vol. 113","author":"Fujita","year":"1988"},{"issue":"11","key":"10.1016\/j.ejcb.2017.02.002_bib0095","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1002\/elps.200800720","article-title":"Quantifying Western blots: pitfalls of densitometry","volume":"30","author":"Gassmann","year":"2009","journal-title":"Electrophoresis"},{"issue":"5","key":"10.1016\/j.ejcb.2017.02.002_bib0100","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1083\/jcb.118.5.1071","article-title":"Isolation of insoluble secretory product from bovine thyroid: extracellular storage of thyroglobulin in covalently cross-linked form","volume":"118","author":"Herzog","year":"1992","journal-title":"J. Cell Biol."},{"issue":"7","key":"10.1016\/j.ejcb.2017.02.002_bib0105","doi-asserted-by":"crossref","first-page":"3974","DOI":"10.1016\/j.bbagen.2012.04.009","article-title":"The pathophysiological consequences of thyroid hormone transporter deficiencies: insights from mouse models","volume":"1830","author":"Heuer","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"issue":"1","key":"10.1016\/j.ejcb.2017.02.002_bib0110","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/elps.1150090106","article-title":"Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels","volume":"9","author":"Heukeshoven","year":"1988","journal-title":"Electrophoresis"},{"key":"10.1016\/j.ejcb.2017.02.002_bib0115","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1471-2091-10-23","article-title":"Monitoring compartment-specific substrate cleavage by cathepsins B, K, L, and S at physiological pH and redox conditions","volume":"10","author":"Jordans","year":"2009","journal-title":"BMC Biochem."},{"issue":"8","key":"10.1016\/j.ejcb.2017.02.002_bib0120","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1093\/bioinformatics\/btr095","article-title":"Improved structure, function and compatibility for CellProfiler: modular high-throughput image analysis software","volume":"27","author":"Kamentsky","year":"2011","journal-title":"Bioinformatics (Oxford, England)"},{"issue":"7","key":"10.1016\/j.ejcb.2017.02.002_bib0125","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1515\/BC.2000.076","article-title":"The thioredoxin boxes of thyroglobulin: possible implications for intermolecular disulfide bond formation in the follicle lumen","volume":"381","author":"Klein","year":"2000","journal-title":"Biol. Chem."},{"issue":"3\u20134","key":"10.1016\/j.ejcb.2017.02.002_bib0130","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/0165-022X(84)90040-X","article-title":"Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose","volume":"10","author":"Kyhse-Andersen","year":"1984","journal-title":"J. Biochem. Biophys. Methods"},{"issue":"21","key":"10.1016\/j.ejcb.2017.02.002_bib0135","doi-asserted-by":"crossref","first-page":"13899","DOI":"10.1074\/jbc.272.21.13899","article-title":"Equistatin, a new inhibitor of cysteine proteinases from Actinia equina, is structurally related to thyroglobulin type-1 domain","volume":"272","author":"Lenarcic","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejcb.2017.02.002_bib0140","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1210\/en.2010-0900","article-title":"Distinct roles of deiodinases on the phenotype of Mct8 defect: a comparison of eight different mouse genotypes","volume":"152","author":"Liao","year":"2011","journal-title":"Endocrinology"},{"issue":"Pt 24","key":"10.1016\/j.ejcb.2017.02.002_bib0145","doi-asserted-by":"crossref","first-page":"4877","DOI":"10.1242\/jcs.00184","article-title":"Trafficking of lysosomal cathepsin B-green fluorescent protein to the surface of thyroid epithelial cells involves the endosomal\/lysosomal compartment","volume":"115","author":"Linke","year":"2002","journal-title":"J. Cell Sci."},{"issue":"5","key":"10.1016\/j.ejcb.2017.02.002_bib0150","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1515\/BC.2002.081","article-title":"Thyroid stimulating hormone upregulates secretion of cathepsin B from thyroid epithelial cells","volume":"383","author":"Linke","year":"2002","journal-title":"Biol. Chem."},{"key":"10.1016\/j.ejcb.2017.02.002_bib0155","first-page":"92","article-title":"Expression pattern of thyroid hormone transporters in the postnatal mouse brain","volume":"5","author":"M\u00fcller","year":"2014","journal-title":"Front. Endocrinol."},{"issue":"24","key":"10.1016\/j.ejcb.2017.02.002_bib0160","doi-asserted-by":"crossref","first-page":"6413","DOI":"10.1113\/jphysiol.2012.239574","article-title":"T-type amino acid transporter TAT1 (Slc16a10) is essential for extracellular aromatic amino acid homeostasis control","volume":"590","author":"Mariotta","year":"2012","journal-title":"J. Physiol."},{"key":"10.1016\/j.ejcb.2017.02.002_bib0165","series-title":"37th Annual Meeting of the European Thyroid Association","first-page":"75","article-title":"Correlation of the expression and localization of thyroid hormone transporters with thyroglobulin procesing cathepsins in mouse thyroid epithelial cells","author":"McInnes","year":"2013"},{"issue":"1","key":"10.1016\/j.ejcb.2017.02.002_bib0170","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1210\/en.2013-1800","article-title":"Tissue-specific alterations in thyroid hormone homeostasis in combined Mct10 and Mct8 deficiency","volume":"155","author":"Muller","year":"2014","journal-title":"Endocrinology"},{"issue":"3","key":"10.1016\/j.ejcb.2017.02.002_bib0175","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1210\/mend.12.3.0069","article-title":"Enhanced binding to the molecular chaperone BiP slows thyroglobulin export from the endoplasmic reticulum","volume":"12","author":"Muresan","year":"1998","journal-title":"Mol. Endocrinol."},{"issue":"11","key":"10.1016\/j.ejcb.2017.02.002_bib0180","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1515\/bchm2.1979.360.2.1657","article-title":"A simple, versatile, sensitive and volume-independent method for quantitative protein determination which is independent of other external influences","volume":"360","author":"Neuhoff","year":"1979","journal-title":"Hoppe-Seyler's Zeitschrift fur Physiologische Chemie"},{"key":"10.1016\/j.ejcb.2017.02.002_bib0185","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/B978-0-12-416021-7.00004-3","article-title":"Mechanisms of thyroid development and dysgenesis: an analysis based on developmental stages and concurrent embryonic anatomy","volume":"106","author":"Nilsson","year":"2013","journal-title":"Curr. Top. Dev. Biol."},{"key":"10.1016\/j.ejcb.2017.02.002_bib0190","series-title":"Endocrinology: An Integrated Approach","article-title":"Chapter 3: the thyroid gland","author":"Nussey","year":"2001"},{"issue":"1\u20132","key":"10.1016\/j.ejcb.2017.02.002_bib0195","doi-asserted-by":"crossref","first-page":"C89","DOI":"10.1016\/0303-7207(91)90176-S","article-title":"The thyroid hormone secretory pathway-current dogmas and alternative hypotheses","volume":"78","author":"Rousset","year":"1991","journal-title":"Mol. Cell. Endocrinol."},{"issue":"7","key":"10.1016\/j.ejcb.2017.02.002_bib0200","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1096\/fasebj.14.7.1005","article-title":"Covalent cross-linking of secreted bovine thyroglobulin by transglutaminase","volume":"14","author":"Saber-Lichtenberg","year":"2000","journal-title":"FASEB J."},{"issue":"23","key":"10.1016\/j.ejcb.2017.02.002_bib0205","doi-asserted-by":"crossref","first-page":"13453","DOI":"10.1073\/pnas.95.23.13453","article-title":"Impaired osteoclastic bone resorption leads to osteopetrosis in cathepsin-K-deficient mice","volume":"95","author":"Saftig","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ejcb.2017.02.002_bib0210","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/S0076-6879(02)48649-1","article-title":"Protein cross-linking by self-assisted intermolecular disulfide bond formation","volume":"348","author":"Schmitz","year":"2002","journal-title":"Methods Enzymol."},{"issue":"10","key":"10.1016\/j.ejcb.2017.02.002_bib0215","doi-asserted-by":"crossref","first-page":"3013","DOI":"10.1016\/j.optcom.2008.01.012","article-title":"Accurate step wedge calibration for densitometry of electrophoresis gels","volume":"281","author":"Tan","year":"2008","journal-title":"Opt. Commun."},{"issue":"Pt 24","key":"10.1016\/j.ejcb.2017.02.002_bib0220","doi-asserted-by":"crossref","first-page":"4487","DOI":"10.1242\/jcs.113.24.4487","article-title":"Cathepsin K in thyroid epithelial cells: sequence, localization and possible function in extracellular proteolysis of thyroglobulin","volume":"113","author":"Tepel","year":"2000","journal-title":"J. Cell Sci."},{"issue":"3","key":"10.1016\/j.ejcb.2017.02.002_bib0225","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1172\/JCI28253","article-title":"Abnormal thyroid hormone metabolism in mice lacking the monocarboxylate transporter 8","volume":"117","author":"Trajkovic","year":"2007","journal-title":"J. Clin. Invest."},{"issue":"2","key":"10.1016\/j.ejcb.2017.02.002_bib0230","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1210\/en.2009-1053","article-title":"Consequences of monocarboxylate transporter 8 deficiency for renal transport and metabolism of thyroid hormones in mice","volume":"151","author":"Trajkovic-Arsic","year":"2010","journal-title":"Endocrinology"},{"issue":"10","key":"10.1016\/j.ejcb.2017.02.002_bib0235","doi-asserted-by":"crossref","first-page":"5053","DOI":"10.1210\/en.2010-0593","article-title":"Impact of monocarboxylate transporter-8 deficiency on the hypothalamus-pituitary-thyroid axis in mice","volume":"151","author":"Trajkovic-Arsic","year":"2010","journal-title":"Endocrinology"},{"issue":"03","key":"10.1016\/j.ejcb.2017.02.002_bib0240","first-page":"P12","article-title":"Functional analysis of the angio-follicular unit of the mouse thyroid gland","volume":"123","author":"Weber","year":"2015","journal-title":"Exp. Clin. Endocrinol. Diabetes"},{"issue":"30","key":"10.1016\/j.ejcb.2017.02.002_bib0245","doi-asserted-by":"crossref","first-page":"9439","DOI":"10.1523\/JNEUROSCI.6055-08.2009","article-title":"Neuronal 3',3,5-triiodothyronine (T3) uptake and behavioral phenotype of mice deficient in Mct8, the neuronal T3 transporter mutated in Allan-Herndon-Dudley syndrome","volume":"29","author":"Wirth","year":"2009","journal-title":"J. Neurosci."},{"issue":"4","key":"10.1016\/j.ejcb.2017.02.002_bib0250","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1530\/EJE-11-0369","article-title":"Monocarboxylate transporter 8 deficiency: altered thyroid morphology and persistent high triiodothyronine\/thyroxine ratio after thyroidectomy","volume":"165","author":"Wirth","year":"2011","journal-title":"Eur. J. Endocrinol.\/Eur. Feder. Endocr. Soc."}],"container-title":["European Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0171933516303041?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0171933516303041?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T10:13:22Z","timestamp":1658744002000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0171933516303041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":50,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["S0171933516303041"],"URL":"https:\/\/doi.org\/10.1016\/j.ejcb.2017.02.002","relation":{},"ISSN":["0171-9335"],"issn-type":[{"value":"0171-9335","type":"print"}],"subject":[],"published":{"date-parts":[[2017,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Interdependence of thyroglobulin processing and thyroid hormone export in the mouse thyroid gland","name":"articletitle","label":"Article Title"},{"value":"European Journal of Cell Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejcb.2017.02.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 The Authors. Published by Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}]}}