{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:02:37Z","timestamp":1790830957007,"version":"4.1.0"},"reference-count":34,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002402","name":"Sun Yat-sen University","doi-asserted-by":"publisher","award":["2023ww601"],"award-info":[{"award-number":["2023ww601"]}],"id":[{"id":"10.13039\/501100002402","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002402","name":"Sun Yat-sen University","doi-asserted-by":"publisher","award":["2019006"],"award-info":[{"award-number":["2019006"]}],"id":[{"id":"10.13039\/501100002402","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016692","name":"Key Research and Development Program of Ningxia","doi-asserted-by":"publisher","award":["2022BEG02040"],"award-info":[{"award-number":["2022BEG02040"]}],"id":[{"id":"10.13039\/100016692","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271148"],"award-info":[{"award-number":["82271148"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U20A20399"],"award-info":[{"award-number":["U20A20399"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82401331"],"award-info":[{"award-number":["82401331"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015956","name":"Special Project for Research and Development in Key areas of Guangdong Province","doi-asserted-by":"publisher","award":["2023B1111040004"],"award-info":[{"award-number":["2023B1111040004"]}],"id":[{"id":"10.13039\/501100015956","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015956","name":"Special Project for Research and Development in Key areas of Guangdong Province","doi-asserted-by":"publisher","award":["2020B0101130015"],"award-info":[{"award-number":["2020B0101130015"]}],"id":[{"id":"10.13039\/501100015956","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2504100"],"award-info":[{"award-number":["2022YFC2504100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Cell Biology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.ejcb.2026.151562","type":"journal-article","created":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T14:02:30Z","timestamp":1786284150000},"page":"151562","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of human nasolacrimal duct epithelial organoid model"],"prefix":"10.1016","volume":"105","author":[{"given":"Mingrui","family":"Pan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaojuan","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qintai","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanwei","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejcb.2026.151562_bib1","series-title":"Atlas of Lacrimal Drainage Disorders","article-title":"The Lacrimal Drainage Anatomy","author":"Ali","year":"2018"},{"key":"10.1016\/j.ejcb.2026.151562_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.preteyeres.2023.101193","article-title":"Etiopathogenesis of primary acquired nasolacrimal duct obstruction (PANDO)","volume":"96","author":"Ali","year":"2023","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.ejcb.2026.151562_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.preteyeres.2023.101193","article-title":"Etiopathogenesis of primary acquired nasolacrimal duct obstruction (PANDO)","volume":"96","author":"Ali","year":"2023","journal-title":"Prog. Retin. Eye Res."},{"issue":"3","key":"10.1016\/j.ejcb.2026.151562_bib4","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1080\/02713683.2019.1580376","article-title":"Human Lacrimal Drainage System Reconstruction, Recanalization, and Regeneration","volume":"45","author":"Ali","year":"2020","journal-title":"Curr. Eye Res."},{"issue":"8","key":"10.1016\/j.ejcb.2026.151562_bib5","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1002\/alr.21345","article-title":"Anatomic relationship of nasolacrimal duct and major lateral wall landmarks: cadaveric study with surgical implications","volume":"4","author":"Ali","year":"2014","journal-title":"Int. Forum Allergy Rhinol."},{"issue":"23","key":"10.1016\/j.ejcb.2026.151562_bib6","doi-asserted-by":"crossref","first-page":"12994","DOI":"10.3390\/ijms222312994","article-title":"The communication between ocular surface and Nasal Epithelia in 3D cell culture technology for translational research: a narrative review","volume":"22","author":"Aydin","year":"2021","journal-title":"Int. J. Mol. Sci."},{"issue":"23","key":"10.1016\/j.ejcb.2026.151562_bib7","doi-asserted-by":"crossref","first-page":"12994","DOI":"10.3390\/ijms222312994","article-title":"The communication between ocular surface and nasal epithelia in 3D cell culture technology for translational research: a narrative review","volume":"22","author":"Aydin","year":"2021","journal-title":"Int. J. Mol. Sci."},{"issue":"7","key":"10.1016\/j.ejcb.2026.151562_bib8","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1016\/j.stem.2021.02.024","article-title":"Exploring the human lacrimal gland using organoids and single-cell sequencing","volume":"28","author":"Bannier-H\u00e9laou\u00ebt","year":"2021","journal-title":"Cell. Stem Cell."},{"issue":"2","key":"10.1016\/j.ejcb.2026.151562_bib9","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.stem.2023.12.008","article-title":"Human conjunctiva organoids to study ocular surface homeostasis and disease","volume":"31","author":"Bannier-H\u00e9laou\u00ebt","year":"2024","journal-title":"Cell. Stem Cell."},{"issue":"9","key":"10.1016\/j.ejcb.2026.151562_bib10","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1038\/s41551-020-0565-2","article-title":"High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays","volume":"4","author":"Brandenberg","year":"2020","journal-title":"Nat. Biomed. Eng."},{"issue":"7","key":"10.1016\/j.ejcb.2026.151562_bib11","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1016\/j.cell.2016.05.082","article-title":"Modeling Development and Disease with Organoids","volume":"165","author":"Clevers","year":"2016","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.ejcb.2026.151562_bib12","doi-asserted-by":"crossref","first-page":"C151","DOI":"10.1152\/ajpcell.00120.2020","article-title":"A brief history of organoids","volume":"319","author":"Corr\u00f2","year":"2020","journal-title":"Am. J. Physiol. Cell. Physiol."},{"issue":"3","key":"10.1016\/j.ejcb.2026.151562_bib13","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1097\/SCS.0000000000006108","article-title":"Relationship between primary acquired nasolacrimal duct obstruction, paranasal abnormalities and nasal septal deviation","volume":"31","author":"Dikici","year":"2020","journal-title":"J. Craniofac Surg."},{"issue":"29","key":"10.1016\/j.ejcb.2026.151562_bib14","doi-asserted-by":"crossref","DOI":"10.12998\/wjcc.v13.i29.108046","article-title":"High-resolution optical coherence tomography for screening ocular surface tumors: historical markers and future directions","volume":"13","author":"Enaholo","year":"2025","journal-title":"World J. Clin. Cases"},{"issue":"5","key":"10.1016\/j.ejcb.2026.151562_bib15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3390\/bios14050225","article-title":"A Comprehensive Review of Organ-on-a-Chip Technology and Its Applications","volume":"14","author":"Farhang Doost","year":"2024","journal-title":"Biosensors"},{"key":"10.1016\/j.ejcb.2026.151562_bib16","series-title":"Principles and Practice of Lacrimal Surgery","article-title":"Anatomy, Physiology, and Immunology of the Lacrimal System","author":"Kakizaki","year":"2018"},{"issue":"6","key":"10.1016\/j.ejcb.2026.151562_bib17","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1007\/s00417-023-06352-2","article-title":"Are inflammation-related diseases risk factors for primary acquired nasolacrimal duct obstruction? A large scale, national case-control study","volume":"262","author":"Kerber","year":"2024","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"issue":"17","key":"10.1016\/j.ejcb.2026.151562_bib18","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2300376120","article-title":"Human airway and nasal organoids reveal escalating replicative fitness of SARS-CoV-2 emerging variants","volume":"120","author":"Li","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"8","key":"10.1016\/j.ejcb.2026.151562_bib19","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1111\/all.13424","article-title":"Role of IL-13R\u03b12 in modulating IL-13-induced MUC5AC and ciliary changes in healthy and CRSwNP mucosa","volume":"73","author":"Liu","year":"2018","journal-title":"Allergy"},{"key":"10.1016\/j.ejcb.2026.151562_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115285","article-title":"Biosensors integrated 3D organoid\/organ-on-a-chip system: A real-time biomechanical, biophysical, and biochemical monitoring and characterization","volume":"231","author":"Liu","year":"2023","journal-title":"Biosens. Bioelectron."},{"issue":"1","key":"10.1016\/j.ejcb.2026.151562_bib21","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1097\/00002341-199203000-00002","article-title":"Clinicopathologic study of lacrimal sac and nasal mucosa in 44 patients with complete acquired nasolacrimal duct obstruction","volume":"8","author":"Mauriello","year":"1992","journal-title":"Ophthalmic Plast. Reconstr. Surg."},{"issue":"1","key":"10.1016\/j.ejcb.2026.151562_bib22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s004290050160","article-title":"Functional anatomy of human lacrimal duct epithelium","volume":"198","author":"Paulsen","year":"1998","journal-title":"Anat. Embryol. (Berl. )"},{"issue":"5","key":"10.1016\/j.ejcb.2026.151562_bib23","first-page":"965","article-title":"The cavernous body of the human efferent tear ducts: function in tear outflow mechanism","volume":"41","author":"Paulsen","year":"2000","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"issue":"2","key":"10.1016\/j.ejcb.2026.151562_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbcan.2021.188527","article-title":"Recent advances in organoid development and applications in disease modeling","volume":"1875","author":"Rauth","year":"2021","journal-title":"Biochim. Biophys. Acta Rev. Cancer"},{"issue":"1","key":"10.1016\/j.ejcb.2026.151562_bib25","doi-asserted-by":"crossref","first-page":"21","DOI":"10.5152\/eurasianjmed.2019.19034","article-title":"Sinonasal anatomical variations and primary acquired nasolacrimal duct obstruction: a single centre, case-control investigation","volume":"52","author":"Samarei","year":"2020","journal-title":"Eurasia J. Med."},{"issue":"1","key":"10.1016\/j.ejcb.2026.151562_bib26","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1038\/s42003-022-03499-2","article-title":"Cell response analysis in SARS-CoV-2 infected bronchial organoids","volume":"5","author":"Sano","year":"2022","journal-title":"Commun. Biol."},{"issue":"7244","key":"10.1016\/j.ejcb.2026.151562_bib27","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1038\/nature07935","article-title":"Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche","volume":"459","author":"Sato","year":"2009","journal-title":"Nature"},{"issue":"5","key":"10.1016\/j.ejcb.2026.151562_bib28","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3928\/0191-3913-19810901-06","article-title":"Development and congenital abnormalities of the nasolacrimal apparatus","volume":"18","author":"Sevel","year":"1981","journal-title":"J. Pediatr. Ophthalmol. Strabismus"},{"key":"10.1016\/j.ejcb.2026.151562_bib29","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.ejpb.2023.01.013","article-title":"Air-liquid interface (ALI) impact on different respiratory cell cultures","volume":"184","author":"Silva","year":"2023","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.ejcb.2026.151562_bib30","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.ejpb.2023.01.013","article-title":"Air-liquid interface (ALI) impact on different respiratory cell cultures","volume":"184","author":"Silva","year":"2023","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.ejcb.2026.151562_bib31","series-title":"Gray's Anatomy Series","article-title":"Gray's Anatomy: The Anatomical Basis of Clinical Practice","author":"Standring","year":"2016"},{"key":"10.1016\/j.ejcb.2026.151562_bib32","doi-asserted-by":"crossref","unstructured":"Georgios Stroulios, Mathieu Hubert, Wing Chang, Allen Eaves, Sharon Louis, Philipp Kramer, Caroline Tapparel, Salvatore Simmini. Generation of apical-out nasal organoids to facilitate viral infection and drug screening bioRxiv 2025.10.07.680885; doi: https:\/\/doi.org\/10.1101\/2025.10.07.680885.","DOI":"10.1101\/2025.10.07.680885"},{"issue":"11","key":"10.1016\/j.ejcb.2026.151562_bib33","doi-asserted-by":"crossref","DOI":"10.1111\/cpr.13704","article-title":"Establishment of human corneal epithelial organoids for ex vivo modelling dry eye disease","volume":"57","author":"Wan","year":"2024","journal-title":"Cell. Prolif."},{"issue":"4","key":"10.1016\/j.ejcb.2026.151562_bib34","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1007\/s00405-024-09193-9","article-title":"Anatomical factors behind acquired primary nasolacrimal duct obstruction and acute dacryocystitis","volume":"282","author":"Yaz\u0131c\u0131o\u011flu","year":"2025","journal-title":"Eur. Arch. Otorhinolaryngol."}],"container-title":["European Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0171933526000336?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0171933526000336?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:35:46Z","timestamp":1790829346000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0171933526000336"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["S0171933526000336"],"URL":"https:\/\/doi.org\/10.1016\/j.ejcb.2026.151562","relation":{},"ISSN":["0171-9335"],"issn-type":[{"value":"0171-9335","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Establishment of human nasolacrimal duct epithelial organoid model","name":"articletitle","label":"Article Title"},{"value":"European Journal of Cell Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejcb.2026.151562","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier GmbH.","name":"copyright","label":"Copyright"}],"article-number":"151562"}}