{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:50:35Z","timestamp":1781535035973,"version":"3.54.5"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007303","name":"University of Balamand","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007303","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000855","name":"University of Birmingham","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000704","name":"Royal Society of Chemistry","doi-asserted-by":"publisher","award":["EP\/J003220\/1"],"award-info":[{"award-number":["EP\/J003220\/1"]}],"id":[{"id":"10.13039\/501100000704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Medicinal Chemistry"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.ejmech.2025.117889","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T11:33:30Z","timestamp":1750764810000},"page":"117889","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Discovery of novel fluorescent amino-pyrazolines that detect and kill Mycobacterium tuberculosis"],"prefix":"10.1016","volume":"297","author":[{"given":"Yixin","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Lanne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreenivas","family":"Avula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariwan A.","family":"Hama Salih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudan","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin","family":"Milne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geraint","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Ritchie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon","family":"Frampton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micky","family":"Tortorella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John S.","family":"Fossey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke J.","family":"Alderwick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2289-3949","authenticated-orcid":false,"given":"Cleopatra","family":"Neagoie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejmech.2025.117889_bib1","series-title":"Global Tuberculosis Report","year":"2023"},{"key":"10.1016\/j.ejmech.2025.117889_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2024.107074","article-title":"Advancements and challenges in tuberculosis drug discovery: a comprehensive overview","volume":"198","author":"Agnivesh","year":"2025","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.ejmech.2025.117889_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejmech.2024.116785","article-title":"Recent advances and challenges of revolutionizing drug-resistant tuberculosis treatment","volume":"277","author":"Wei","year":"2024","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib4","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1093\/cid\/cix992","article-title":"Bedaquiline resistance: its emergence, mechanism, and prevention","volume":"66","author":"Nguyen","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ejmech.2025.117889_bib5","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.cmi.2021.08.007","article-title":"Pretomanid for tuberculosis: a systematic review","volume":"28","author":"Gils","year":"2022","journal-title":"Clin. Microbiol. Infection"},{"key":"10.1016\/j.ejmech.2025.117889_bib6","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1056\/NEJMoa1112433","article-title":"Delamanid for multidrug-resistant pulmonary tuberculosis","volume":"366","author":"Gler","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.ejmech.2025.117889_bib7","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1093\/jac\/dkaa136","article-title":"Systematic review of mutations associated with resistance to the new and repurposed mycobacterium tuberculosis drugs bedaquiline, clofazimine, linezolid, delamanid and pretomanid","volume":"75","author":"Kadura","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.ejmech.2025.117889_bib8","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1021\/acs.jmedchem.3c01643","article-title":"Azetidines kill multidrug-resistant mycobacterium tuberculosis without detectable resistance by blocking mycolate assembly","volume":"67","author":"Cui","year":"2024","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib9","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.2174\/1871520620666200705214917","article-title":"Nitrogen-containing heterocycles as anticancer agents: an overview","volume":"20","author":"Lang","year":"2020","journal-title":"Anti-Cancer Agent Med Chem- Anti-Cancer Agent"},{"key":"10.1016\/j.ejmech.2025.117889_bib10","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1021\/acs.jmedchem.3c01703","article-title":"Discovery of N-(1-(6-oxo-1,6-dihydropyrimidine)-pyrazole) acetamide derivatives as novel noncovalent DprE1 inhibitors against mycobacterium tuberculosis","volume":"67","author":"Yang","year":"2024","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejmech.2020.112666","article-title":"Recent advancements in the development of bioactive pyrazoline derivatives","volume":"205","author":"Nehra","year":"2020","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib12","doi-asserted-by":"crossref","first-page":"154","DOI":"10.2174\/157489109789318569","article-title":"Biological activities of pyrazoline derivatives -a recent development","volume":"4","author":"Kumar","year":"2009","journal-title":"Recent Pat. Anti-Infect. Drug Discov."},{"key":"10.1016\/j.ejmech.2025.117889_bib13","doi-asserted-by":"crossref","first-page":"921","DOI":"10.2174\/1389557511313060012","article-title":"Pyrazolines: a biological review","volume":"13","author":"Marella","year":"2013","journal-title":"Mini Rev. Med. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib14","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.2174\/1871520618666180313153407","article-title":"2-pyrazolines as biologically active and fluorescent agents, an overview","volume":"18","author":"Singh","year":"2018","journal-title":"Anti-Cancer Agent Med Chem- Anti-Cancer Agent"},{"key":"10.1016\/j.ejmech.2025.117889_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.poly.2024.117028","article-title":"Synthesis, characterization, and antimicrobial activity of novel ferrocene-containing 1-(thio)acetyl pyrazolines","volume":"258","author":"Todosijevi\u0107","year":"2024","journal-title":"Polyhedron"},{"key":"10.1016\/j.ejmech.2025.117889_bib16","doi-asserted-by":"crossref","first-page":"500","DOI":"10.4274\/tjps.galenos.2019.42650","article-title":"Antimicrobial activities of some pyrazoline and hydrazone derivatives","volume":"17","author":"Evranos Aks\u00f6z","year":"2020","journal-title":"Turk. J. Pharm. Sci."},{"key":"10.1016\/j.ejmech.2025.117889_bib17","doi-asserted-by":"crossref","first-page":"5319","DOI":"10.3390\/ijms24065319","article-title":"Antimicrobial evaluation of new pyrazoles, indazoles and pyrazolines prepared in continuous flow mode","volume":"24","author":"Burke","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.ejmech.2025.117889_bib18","article-title":"Synthesis and anti-inflammatory activity of pyrazolines","volume":"7","author":"Ramesh","year":"2010","journal-title":"J. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib19","doi-asserted-by":"crossref","first-page":"3439","DOI":"10.3390\/molecules26113439","article-title":"Pyrazoles and pyrazolines as anti-inflammatory agents","volume":"26","author":"Mantzanidou","year":"2021","journal-title":"Molecules"},{"key":"10.1016\/j.ejmech.2025.117889_bib20","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.bmc.2018.12.012","article-title":"Synthesis of novel pyrazoline derivatives and the evaluation of death mechanisms involved in their antileukemic activity","volume":"27","author":"Stefanes","year":"2019","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib21","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.3390\/molecules23092151","article-title":"Design, synthesis, and neuroprotective effects of a series of pyrazolines against 6-hydroxydopamine-induced oxidative stress","volume":"23","author":"\u00d6zdemir","year":"2018","journal-title":"Molecules"},{"key":"10.1016\/j.ejmech.2025.117889_bib22","doi-asserted-by":"crossref","first-page":"38207","DOI":"10.1021\/acsomega.2c05339","article-title":"Pyrazoline containing compounds as therapeutic targets for neurodegenerative disorders","volume":"7","author":"Ahsan","year":"2022","journal-title":"ACS Omega"},{"key":"10.1016\/j.ejmech.2025.117889_bib23","doi-asserted-by":"crossref","first-page":"5507","DOI":"10.3390\/ijms21155507","article-title":"Pyrazoline hybrids as promising anticancer agents: an up-to-date overview","volume":"21","author":"Matiadis","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.ejmech.2025.117889_bib24","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1016\/j.ejmech.2008.09.032","article-title":"Synthesis of novel thiazolone-based compounds containing pyrazoline moiety and evaluation of their anticancer activity","volume":"44","author":"Havrylyuk","year":"2009","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib25","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.ejmech.2009.09.032","article-title":"Pyrazoline bearing benzimidazoles: search for anticancer agent","volume":"45","author":"Shaharyar","year":"2010","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib26","doi-asserted-by":"crossref","first-page":"5044","DOI":"10.1021\/ol102215e","article-title":"Diastereoselective preparation of azetidines and pyrrolidines","volume":"12","author":"Feula","year":"2010","journal-title":"Org. Lett."},{"key":"10.1016\/j.ejmech.2025.117889_bib27","doi-asserted-by":"crossref","first-page":"5083","DOI":"10.1039\/c3ob41007b","article-title":"Synthesis of azetidines and pyrrolidines via iodocyclisation of homoallyl amines and exploration of activity in a zebrafish embryo assay","volume":"11","author":"Feula","year":"2013","journal-title":"Org. Biomol. Chem."},{"key":"10.1016\/j.ejmech.2025.117889_bib28","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1039\/C5QO00183H","article-title":"\u03b3-lactams and furan bispyrrolidines via iodine mediated cyclisation of homoallylamines","volume":"2","author":"Salih","year":"2015","journal-title":"Org. Chem. Front."},{"key":"10.1016\/j.ejmech.2025.117889_bib29","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1039\/c39760000734","article-title":"Rules for ring closure","volume":"0","author":"Baldwin","year":"1976","journal-title":"J. Chem. Soc., Chem. Commun."},{"key":"10.1016\/j.ejmech.2025.117889_bib30","doi-asserted-by":"crossref","first-page":"4471","DOI":"10.1007\/s00018-016-2302-2","article-title":"Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action","volume":"73","author":"Cushnie","year":"2016","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.ejmech.2025.117889_bib31","first-page":"11","article-title":"How do bacteria localize proteins to the cell pole?","volume":"127","author":"Laloux","year":"2014","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ejmech.2025.117889_bib32","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1073\/pnas.0305439101","article-title":"A polyketide synthase catalyzes the last condensation step of mycolic acid biosynthesis in mycobacteria and related organisms","volume":"101","author":"Portevin","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ejmech.2025.117889_bib33","first-page":"1660","article-title":"The polyketide synthase Pks13 catalyzes a novel mechanism of lipid transfer in mycobacteria","volume":"32","author":"Malaga","year":"2014","journal-title":"Cell Press"},{"key":"10.1016\/j.ejmech.2025.117889_bib34","doi-asserted-by":"crossref","first-page":"11241","DOI":"10.1073\/pnas.1901346116","article-title":"MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine","volume":"166","author":"Su","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ejmech.2025.117889_bib35","doi-asserted-by":"crossref","first-page":"7993","DOI":"10.1073\/pnas.1700062114","article-title":"MmpL3 is the flippase for mycolic acids in mycobacteria","volume":"114","author":"Xu","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ejmech.2025.117889_bib36","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1126\/science.276.5317.1420","article-title":"Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis","volume":"276","author":"Belisle","year":"1997","journal-title":"Science"},{"key":"10.1016\/j.ejmech.2025.117889_bib42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nmicrobiol.2015.6","article-title":"THPP target assignment reveals EchA6 as an essential fatty acid shuttle in mycobacteria","volume":"1","author":"Cox","year":"2016","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.ejmech.2025.117889_bib43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-017-09642-y","article-title":"Inhibiting mycobacterial tryptophan synthase by targeting the inter-subunit interface","volume":"7","author":"Abrahams","year":"2017","journal-title":"Sci. Rep."}],"container-title":["European Journal of Medicinal Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0223523425006543?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0223523425006543?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T04:58:50Z","timestamp":1776920330000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0223523425006543"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":38,"alternative-id":["S0223523425006543"],"URL":"https:\/\/doi.org\/10.1016\/j.ejmech.2025.117889","relation":{},"ISSN":["0223-5234"],"issn-type":[{"value":"0223-5234","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Discovery of novel fluorescent amino-pyrazolines that detect and kill Mycobacterium tuberculosis","name":"articletitle","label":"Article Title"},{"value":"European Journal of Medicinal Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejmech.2025.117889","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Masson SAS.","name":"copyright","label":"Copyright"}],"article-number":"117889"}}