{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T01:14:57Z","timestamp":1788052497439,"version":"build-2784847793"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100024370","name":"Governo Italiano Ministero dell'Istruzione dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024370","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Medicinal Chemistry"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.ejmech.2026.119195","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:03:37Z","timestamp":1785251017000},"page":"119195","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Targeting oxysterol-binding protein (OSBP) as a novel strategy to inhibit herpes simplex virus 2 (HSV-2) replication: First structure-activity relationship study of antiviral cholenamide derivatives"],"prefix":"10.1016","volume":"318","author":[{"given":"Andrea","family":"Civra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Nigro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domiziana","family":"Porporato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Costantino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Bianconi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Zambri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"De March","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"De Conto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Carosati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Macchiarulo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lembo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5994-9390","authenticated-orcid":false,"given":"Maura","family":"Marinozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejmech.2026.119195_bib1","first-page":"315","article-title":"Herpes simplex virus : global infection prevalence and incidence estimates, 2016","volume":"98","author":"James","year":"2020","journal-title":"Bull. WHO"},{"key":"10.1016\/j.ejmech.2026.119195_bib2","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1038\/nm.3103","article-title":"Rapid host immune response and viral dynamics in Herpes simplex Virus-2 infection","volume":"19","author":"Schiffer","year":"2013","journal-title":"Nat. Med."},{"key":"10.1016\/j.ejmech.2026.119195_bib3","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1001\/jama.2011.420","article-title":"Genital shedding of Herpes simplex virus among symptomatic and asymptomatic persons with HSV-2 infection","volume":"305","author":"Tronstein","year":"2011","journal-title":"JAMA"},{"key":"10.1016\/j.ejmech.2026.119195_bib4","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1097\/01.AOG.0000180779.35572.3a","article-title":"Genital herpes complicating pregnancy","volume":"106","author":"Brown","year":"2005","journal-title":"Obstet. Gynecol."},{"key":"10.1016\/j.ejmech.2026.119195_bib5","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1053\/j.semperi.2018.02.004","article-title":"Neonatal Herpes simplex virus infections","volume":"42","author":"Pinninti","year":"2018","journal-title":"Semin. Perinatol."},{"key":"10.1016\/j.ejmech.2026.119195_bib6","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/S0140-6736(11)61750-9","article-title":"Standard-dose and high-dose daily antiviral therapy for short episodes of genital HSV-2 reactivation: three randomised, open-label, cross-over trials","volume":"379","author":"Johnston","year":"2012","journal-title":"Lancet"},{"key":"10.1016\/j.ejmech.2026.119195_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ijos.2016.3","article-title":"New strategies against drug resistance to Herpes simplex virus","volume":"8","author":"Jiang","year":"2016","journal-title":"Int. J. Oral Sci."},{"key":"10.1016\/j.ejmech.2026.119195_bib8","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/S1473-3099(08)70181-6","article-title":"Herpes simplex virus and HIV-1 : deciphering viral synergy","volume":"8","author":"Van De Perre","year":"2008","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.ejmech.2026.119195_bib9","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1016\/S1473-3099(17)30405-X","article-title":"Effect of HSV-2 infection on subsequent HIV acquisition: an updated systematic review and meta-analysis","volume":"17","author":"Looker","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.ejmech.2026.119195_bib10","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.immuni.2012.11.004","article-title":"The transcription factor STAT-1 couples macrophage synthesis of 25-Hydroxycholesterol to the interferon antiviral response","volume":"38","author":"Blanc","year":"2013","journal-title":"Immunity"},{"key":"10.1016\/j.ejmech.2026.119195_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2022.105368","article-title":"27-Hydroxycholesterol inhibits rhinovirus replication in vitro and on human nasal and bronchial histocultures without selecting viral resistant variants","volume":"204","author":"Civra","year":"2022","journal-title":"Antivir. Res."},{"key":"10.1016\/j.ejmech.2026.119195_bib12","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.redox.2017.03.016","article-title":"Inhibition of herpes Simplex-1 virus replication by 25-Hydroxycholesterol and 27-Hydroxycholesterol","volume":"12","author":"Cagno","year":"2017","journal-title":"Redox Biol."},{"key":"10.1016\/j.ejmech.2026.119195_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2023.105634","article-title":"Identification of oxysterol synthetic analogs as a novel class of late-stage inhibitors of Herpes simplex virus 2 replication","volume":"215","author":"Civra","year":"2023","journal-title":"Antivir. Res."},{"key":"10.1016\/j.ejmech.2026.119195_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2025.106113","article-title":"25R,26-Hydroxycholesterol and an oxysterol synthetic analog inhibit Varicella zoster virus replication","volume":"236","author":"Civra","year":"2025","journal-title":"Antivir. Res."},{"key":"10.1016\/j.ejmech.2026.119195_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemphyslip.2021.105151","article-title":"In search for novel liver X receptors modulators by extending the structure\u2013activity relationships of cholenamide derivatives","volume":"241","author":"Pontini","year":"2021","journal-title":"Chem. Phys. Lipids"},{"key":"10.1016\/j.ejmech.2026.119195_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioorg.2024.107922","article-title":"Cholenamide-based, antiviral fluorescent probes targeting oxysterol-binding protein","volume":"153","author":"Nigro","year":"2024","journal-title":"Bioorg. Chem."},{"key":"10.1016\/j.ejmech.2026.119195_bib17","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1139\/v56-130","article-title":"The conversion of hyodesoxycholic acid to progesterone","volume":"34","author":"Bharucha","year":"1956","journal-title":"Can. J. Chem."},{"key":"10.1016\/j.ejmech.2026.119195_bib18","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1080\/00304948.2021.1974260","article-title":"Novel synthetic access to 24a-Homochol-5-en-24a-oate scaffold from stigmasterol","volume":"53","author":"Pontini","year":"2021","journal-title":"Org. Prep. Proced. Int."},{"key":"10.1016\/j.ejmech.2026.119195_bib19","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.celrep.2014.12.054","article-title":"Itraconazole inhibits enterovirus replication by targeting the oxysterol-binding protein","volume":"10","author":"Strating","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.ejmech.2026.119195_bib20","doi-asserted-by":"crossref","first-page":"19678","DOI":"10.1073\/pnas.0603873103","article-title":"Why molecules move along a temperature gradient","volume":"103","author":"Duhr","year":"2006","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.ejmech.2026.119195_bib21","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.molstruc.2014.03.009","article-title":"MicroScale thermophoresis: interaction analysis and beyond","volume":"1077","author":"Jerabek-Willemsen","year":"2014","journal-title":"J. Mol. Struct."},{"key":"10.1016\/j.ejmech.2026.119195_bib22","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1021\/acscentsci.4c01657","article-title":"Identification of a privileged scaffold for inhibition of sterol transport proteins through the synthesis and ring distortion of diverse, pseudo-natural products","volume":"11","author":"Bro","year":"2025","journal-title":"ACS Cent. Sci."},{"key":"10.1016\/j.ejmech.2026.119195_bib23","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/j.cell.2013.09.056","article-title":"A four-step cycle driven by PI(4)P hydrolysis directs sterol\/PI(4)P exchange by the ER-Golgi tether OSBP","volume":"255","author":"Mesmin","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.ejmech.2026.119195_bib24","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1146\/annurev-biochem-061516-044924","article-title":"The oxysterol-binding protein cycle: burning off PI(4)P to transport cholesterol","volume":"87","author":"Antonny","year":"2018","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.ejmech.2026.119195_bib25","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ceb.2017.02.005","article-title":"Viral rewiring of cellular lipid metabolism to create membranous replication compartments","volume":"47","author":"Strating","year":"2017","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.ejmech.2026.119195_bib26","doi-asserted-by":"crossref","first-page":"6503","DOI":"10.1021\/acs.chemrev.5b00590","article-title":"Protein allostery and conformational dynamics","volume":"116","author":"Guo","year":"2016","journal-title":"Chem. Rev."},{"key":"10.1016\/j.ejmech.2026.119195_bib27","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.sbi.2020.01.017","article-title":"Allostery of multidomain proteins with disordered linkers","volume":"62","author":"Huang","year":"2020","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.ejmech.2026.119195_bib28","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1093\/glycob\/cwh085","article-title":"Brefeldin A and filipin distinguish two globotriaosyl ceramide\/verotoxin-1 intracellular trafficking pathways involved in vero cell cytotoxicity","volume":"14","author":"Khine","year":"2004","journal-title":"Glicobiology"},{"key":"10.1016\/j.ejmech.2026.119195_bib29","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/s11745-009-3305-7","article-title":"Cholesterol synthesis inhibitor U18666A and the role of sterol metabolism and trafficking in numerous pathophysiological processes","volume":"44","author":"Cenedella","year":"2009","journal-title":"Lipids"},{"key":"10.1016\/j.ejmech.2026.119195_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2019.104548","article-title":"Differing activities of oxysterol-binding protein (OSBP) targeting anti-viral compounds","volume":"170","author":"Roberts","year":"2019","journal-title":"Antivir. Res."},{"key":"10.1016\/j.ejmech.2026.119195_bib31","doi-asserted-by":"crossref","first-page":"e01904","DOI":"10.1128\/JVI.01904-16","article-title":"Adenovirus modulates toll-like receptor 4 signaling by reprogramming ORP1L-VAP protein contacts for cholesterol transport from endosomes to the endoplasmic reticulum","volume":"91","author":"Cianciola","year":"2017","journal-title":"J. Virol."},{"key":"10.1016\/j.ejmech.2026.119195_bib32","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1021\/acsinfecdis.2c00108","article-title":"Ligand recognition by the lipid transfer domain of human OSBP is important for enterovirus replication","volume":"8","author":"Kobayashi","year":"2022","journal-title":"ACS Infect. Dis."},{"key":"10.1016\/j.ejmech.2026.119195_bib33","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.ejmech.2026.119195_bib34","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1021\/acs.jcim.1c00203","article-title":"AutoDock vina 1.2.0: new docking methods, expanded force field, and Python bindings","volume":"61","author":"Eberhardt","year":"2021","journal-title":"J. Chem. Inf. Model."},{"key":"10.1016\/j.ejmech.2026.119195_bib37","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/nar\/28.1.235","article-title":"The protein data bank","volume":"28","author":"Berman","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ejmech.2026.119195_bib38","doi-asserted-by":"crossref","first-page":"W296","DOI":"10.1093\/nar\/gky427","article-title":"SWISS-MODEL: homology modelling of protein structures and complexes","volume":"46","author":"Waterhouse","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ejmech.2026.119195_bib40","doi-asserted-by":"crossref","first-page":"2882","DOI":"10.1002\/jcc.26054","article-title":"AutoGridFR: improvements on AutoDock affinity maps and associated software tools","volume":"40","author":"Zhang","year":"2019","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.ejmech.2026.119195_bib42","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/ng.625","article-title":"Genome-Wide Association Study identifies variants in the MHC class I, IL10, and IL23R-IL12RB2 regions associated with Beh\u00e7et\u2019s disease","volume":"42","author":"Remmers","year":"2010","journal-title":"Nat. Genet."},{"key":"10.1016\/j.ejmech.2026.119195_bib43","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.jmgm.2005.12.005","article-title":"Automatic atom type and bond type perception in molecular mechanical calculations","volume":"25","author":"Wang","year":"2006","journal-title":"J. Mol. Graph. Model."},{"key":"10.1016\/j.ejmech.2026.119195_bib44","doi-asserted-by":"crossref","first-page":"6183","DOI":"10.1021\/acs.jcim.3c01153","article-title":"AmberTools","volume":"63","author":"Case","year":"2023","journal-title":"J. Chem. Inf. Model."},{"key":"10.1016\/j.ejmech.2026.119195_bib45","doi-asserted-by":"crossref","first-page":"7835","DOI":"10.1021\/acs.jcim.5c01063","article-title":"Recent developments in amber biomolecular simulations","volume":"65","author":"Case","year":"2025","journal-title":"J. Chem. Inf. Model."},{"key":"10.1016\/j.ejmech.2026.119195_bib46","series-title":"Physics Computing Vol. 92","first-page":"252","article-title":"Gromacs: a parallel computer for molecular dynamics simulations","author":"Bekker","year":"1993"},{"key":"10.1016\/j.ejmech.2026.119195_bib47","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.1002\/prot.22711","article-title":"Improved side-chain torsion potentials for the amber Ff99SB protein force field","volume":"78","author":"Lindorff-Larsen","year":"2010","journal-title":"Proteins"},{"key":"10.1016\/j.ejmech.2026.119195_bib48","doi-asserted-by":"crossref","DOI":"10.1063\/5.0013849","article-title":"A protocol for preparing explicitly solvated systems for stable molecular dynamics simulations","volume":"153","author":"Roe","year":"2020","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.ejmech.2026.119195_bib49","unstructured":"https:\/\/doi.org\/10.5281\/zenodo.16992569."}],"container-title":["European Journal of Medicinal Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0223523426006409?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0223523426006409?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:21:04Z","timestamp":1788049264000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0223523426006409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":45,"alternative-id":["S0223523426006409"],"URL":"https:\/\/doi.org\/10.1016\/j.ejmech.2026.119195","relation":{},"ISSN":["0223-5234"],"issn-type":[{"value":"0223-5234","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Targeting oxysterol-binding protein (OSBP) as a novel strategy to inhibit herpes simplex virus 2 (HSV-2) replication: First structure-activity relationship study of antiviral cholenamide derivatives","name":"articletitle","label":"Article Title"},{"value":"European Journal of Medicinal Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejmech.2026.119195","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Masson SAS.","name":"copyright","label":"Copyright"}],"article-number":"119195"}}