{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:10:13Z","timestamp":1790611813260,"version":"4.1.0"},"reference-count":43,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","ejog.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Obstetrics & Gynecology and Reproductive Biology"],"published-print":{"date-parts":[[2008,11]]},"DOI":"10.1016\/j.ejogrb.2008.06.004","type":"journal-article","created":{"date-parts":[[2008,8,13]],"date-time":"2008-08-13T17:14:07Z","timestamp":1218647647000},"page":"39-43","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"title":["The effect of condition\/state of testicular spermatozoa injected to the outcome of TESE\u2013ICSI\u2013ET cycles"],"prefix":"10.1016","volume":"141","author":[{"given":"Janos","family":"Konc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Kany\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandor","family":"Cseh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejogrb.2008.06.004_bib1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0140-6736(92)92425-F","article-title":"Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte","volume":"340","author":"Palermo","year":"1992","journal-title":"Lancet"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib2","series-title":"Principles and practice of assisted human reproduction","author":"Edwards","year":"1995"},{"issue":"12","key":"10.1016\/j.ejogrb.2008.06.004_bib3","doi-asserted-by":"crossref","first-page":"2822","DOI":"10.1093\/humrep\/deh490","article-title":"Should diagnostic testicular sperm retrieval followed by cryopreservation for later ICSI be the procedure of choice for all patients with non-obstructive azoospermia?","volume":"19","author":"Verheyen","year":"2004","journal-title":"Hum Reprod"},{"issue":"7","key":"10.1016\/j.ejogrb.2008.06.004_bib4","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1093\/humrep\/14.7.1794","article-title":"Testicular sperm retrieval and cryopreservation prior to initiating ovarian stimulation as the first line approach in patients with non-obstructive azoospermia","volume":"14","author":"Ben-Yosef","year":"1999","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib5","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S0022-5347(01)62079-2","article-title":"Tesicular sperm extraction with intracytoplasmic sperm injection for nonobstructive azoospermia: testicular histology can predict success of sperm retrieval","volume":"161","author":"Lin-Ming","year":"1999","journal-title":"J Urol"},{"issue":"4","key":"10.1016\/j.ejogrb.2008.06.004_bib6","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/S0015-0282(16)57486-X","article-title":"Using ejaculated, fresh and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection","volume":"63","author":"Nagy","year":"1995","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib7","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S1472-6483(10)60839-5","article-title":"Crude cumulative delivery rate following ICSI using intentionally frozen-thawed testicular spermatozoa in 51 men with non-obstructive azoospermia","volume":"11","author":"Giorgetti","year":"2005","journal-title":"Reprod BioMed Online"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib8","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1093\/humrep\/deg346","article-title":"Cumulative delivery rates after ICSI treatment cycles with freshly retrieved testicular sperm: a 7 year follow-up study","volume":"18","author":"Osmanagaoglu","year":"2003","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib9","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/S0015-0282(97)00358-0","article-title":"Intracytoplasmic injection of fresh and cryopreserved testicular spermatozoa in patients with nonobstructive azoospermia\u2014a comparative study","volume":"68","author":"Friedler","year":"1997","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib10","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1439-0272.2004.00649.x","article-title":"Intracytoplasmic injection with motile and immotile frozen-thawed teticular spermatozoa (the Hungarian experiences)","volume":"35","author":"Matyas","year":"2005","journal-title":"Andrologia"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib11","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1046\/j.1439-0272.2002.00503.x","article-title":"Five years experiences with microinjection of testicular spermatozoa into oocytes in Hungary","volume":"34","author":"Matyas","year":"2002","journal-title":"Andrologia"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib12","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/j.fertnstert.2004.02.116","article-title":"Use of surgical sperm retrieval in azoospermic men: a meta-analysis","volume":"82","author":"Nicopoullos","year":"2004","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib13","unstructured":"Packing slip of SpermFreeze Solution (FertiPro, Beemen, Belgium), 2005, indicating the recommended freezing procedure."},{"key":"10.1016\/j.ejogrb.2008.06.004_bib14","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/S0301-2115(03)00215-X","article-title":"Follow-up study of children born after diode laser assisted hatching","volume":"100","author":"Kany\u00f3","year":"2003","journal-title":"Eur J Obst Gynecol Reprod Biol"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib15","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1023\/B:JARG.0000046202.00570.1d","article-title":"Visualization and examination of the meiotic spindle in human oocytes with polscope","volume":"10","author":"Konc","year":"2004","journal-title":"J Assist Reprod Genet"},{"issue":"1","key":"10.1016\/j.ejogrb.2008.06.004_bib16","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1556\/avet.52.2004.1.11","article-title":"Assisted reproductive research: Laser assisted hatching and spindle detection (spindle view technique)","volume":"52","author":"Kany\u00f3","year":"2004","journal-title":"Acta Vet Hung"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib17","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1093\/humrep\/14.3.749","article-title":"In vitro fertilization treatment for severe male factor: the fertilization potential of immotile spermatozoa obtained by testicular extraction","volume":"14","author":"Shulman","year":"1999","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib18","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1023\/A:1009435732258","article-title":"Pentoxifylline initiates motility in spontaneously immotile epididymal and testicular spermatozoa and allows normal fertilization, pregnancy, and birth after intracytoplasmic sperm injection","volume":"17","author":"Terriou","year":"2000","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib19","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/S1472-6483(10)60974-1","article-title":"Interest of pentoxifylline in ICSI with frozen-thawed testicular spermatozoa from patients with non-obstructive azoospermia","volume":"12","author":"Griveau","year":"2005","journal-title":"Reprod BioMed Online"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib20","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1093\/humrep\/13.4.890","article-title":"Correlation between motility of testicular spermatozoa, testicular histology and the outcome of intracytoplasmic sperm injection","volume":"13","author":"Nagy","year":"1998","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib21","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1097\/00005392-199904010-00372","article-title":"Microdissection TESE: testicular sperm retrieval in non-obstructive azoospermia","volume":"161","author":"Schlegel","year":"1999","journal-title":"J Urol Suppl"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib22","doi-asserted-by":"crossref","first-page":"2630","DOI":"10.1093\/oxfordjournals.humrep.a135758","article-title":"Analysis of 76 total fertilization failure cycles out of 2732 intracytoplasmic sperm injection cycles","volume":"10","author":"Liu","year":"1995","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib23","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/S0090-4295(97)00032-0","article-title":"Testicular sperm extraction with intracytoplasmic sperm injection for nonobstructive azoospermia","volume":"49","author":"Schlegel","year":"1997","journal-title":"Urology"},{"issue":"4","key":"10.1016\/j.ejogrb.2008.06.004_bib24","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1093\/humrep\/14.4.1034","article-title":"Diagnostic testicular biopsy and cryopreservation of testicular tissue as an alternative to repeated surgical openings in the treatment of azoospermic men","volume":"14","author":"Gianaroli","year":"1999","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib25","doi-asserted-by":"crossref","first-page":"13501","DOI":"10.1073\/pnas.241517598","article-title":"Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa","volume":"98","author":"Kusakabe","year":"2001","journal-title":"PNAS"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib26","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1095\/biolreprod.103.025957","article-title":"Freeze-dried sperm fertilization leads to full-term development in rabbits","volume":"70","author":"Liu","year":"2004","journal-title":"Biol Reprod"},{"issue":"3","key":"10.1016\/j.ejogrb.2008.06.004_bib27","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/S0015-0282(03)00798-2","article-title":"Influence of motility on the outcome of in vitro fertilization\/intracytoplasmic sperm injection with fresh vs. frozen testicular sperm from men with obstructive azoospermia","volume":"80","author":"Park","year":"2003","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib28","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1007\/BF02766832","article-title":"Effect of pentoxifylline on immoile testicular spermatozoa","volume":"15","author":"Tasdemir","year":"1998","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib29","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1093\/humrep\/dei249","article-title":"The use of the modified hypo-osotic swelling test for the selection of immotile testicular spermatozoa in patients treated with ICSI: a randomized controlled study","volume":"20","author":"Sallam","year":"2005","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib30","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1016\/S0015-0282(16)58271-5","article-title":"The hypoosmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia","volume":"65","author":"Casper","year":"1996","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib31","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1016\/S0015-0282(98)00351-3","article-title":"Plasma membrane integrity of cryopreserved human sperm: an investigation of the results of the hypoosmotic swelling test, the water test, and eosin-Y staining","volume":"70","author":"Lin","year":"1998","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib32","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1093\/humrep\/12.5.1111","article-title":"Establishment of an optimal hypo-osmotic swelling test by examining single spermatozoa in four different hypo-osmotic solutions","volume":"12","author":"Tsai","year":"1997","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib33","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/S0015-0282(97)81444-6","article-title":"Pregnancy and birth after intracytoplasmic sperm injection with totally immotile sperm recovered from the ejaculate","volume":"67","author":"Barros","year":"1997","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib34","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/BF01239219","article-title":"Chemical removal of the outside of the zona pellucida of day 3 human embryos has no impact on implantation rate","volume":"10","author":"Tucker","year":"1993","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib35","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1093\/HUMREP\/11.3.590","article-title":"Zona thinning with the use of laser: a new approach to assisted hatching in humans","volume":"11","author":"Antinori","year":"1996","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib36","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1093\/oxfordjournals.humrep.a137720","article-title":"Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis","volume":"7","author":"Cohen","year":"1992","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib37","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/BF02068864","article-title":"Does assisted hatching improve implantation rates after in vitro fertilization or intracytoplasmic sperm injection in all patients? A prospective randomized study","volume":"13","author":"Hellebaut","year":"1996","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib38","doi-asserted-by":"crossref","first-page":"2434","DOI":"10.1093\/oxfordjournals.humrep.a019131","article-title":"Enhancement of outcome from intracytoplasmic sperm injection: does co-culture or assisted hatching improve implantation rates?","volume":"11","author":"Tucker","year":"1996","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib39","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1016\/S0015-0282(97)81404-5","article-title":"Assisted hatching increases the implantation and pregnancy rate of in vitro fertilization (IVF)-embryo transfer (ET), but not that of IVF-tubal ET inpatients with repeated IVF failures","volume":"67","author":"Chao","year":"1997","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib40","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1016\/S0015-0282(99)00131-4","article-title":"Clinical application of a new assisted hatching method using a piezo-micromanipulator for morphologically low-quality embryos in poor-prognosis infertility patients","volume":"71","author":"Nakayama","year":"1999","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib41","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1093\/humrep\/15.5.1061","article-title":"Transfer of zona free embryos improves outcome in poor prognosis patients: a prospective randomized controlled study","volume":"15","author":"Mansour","year":"2000","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib42","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1007\/BF02766826","article-title":"Assisted hatching does not enhance IVF success in good prognosis patients","volume":"15","author":"Hurst","year":"1998","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.ejogrb.2008.06.004_bib43","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1093\/humrep\/del124","article-title":"A randomized double-blind controlled study on the efficiency of laser zona pellucida thinning on live birth rates in cases of advanced female age","volume":"21","author":"Frydman","year":"2006","journal-title":"Hum Reprod"}],"container-title":["European Journal of Obstetrics & Gynecology and Reproductive Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S030121150800239X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S030121150800239X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T05:18:57Z","timestamp":1759555137000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S030121150800239X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,11]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,11]]}},"alternative-id":["S030121150800239X"],"URL":"https:\/\/doi.org\/10.1016\/j.ejogrb.2008.06.004","relation":{},"ISSN":["0301-2115"],"issn-type":[{"value":"0301-2115","type":"print"}],"subject":[],"published":{"date-parts":[[2008,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The effect of condition\/state of testicular spermatozoa injected to the outcome of TESE\u2013ICSI\u2013ET cycles","name":"articletitle","label":"Article Title"},{"value":"European Journal of Obstetrics & Gynecology and Reproductive Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejogrb.2008.06.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2008 Elsevier Ireland Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}