{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T14:53:28Z","timestamp":1789829608503,"version":"4.0.1"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzhen","doi-asserted-by":"publisher","award":["SZSM201502035"],"award-info":[{"award-number":["SZSM201502035"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFC1003900\/2018YFC1003904"],"award-info":[{"award-number":["2018YFC1003900\/2018YFC1003904"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","ejog.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Obstetrics &amp; Gynecology and Reproductive Biology"],"published-print":{"date-parts":[[2023,2]]},"DOI":"10.1016\/j.ejogrb.2022.12.012","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T11:15:07Z","timestamp":1670411707000},"page":"68-75","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Prolong cryopreservation duration negatively affects pregnancy outcomes of vitrified-warmed blastocyst transfers using an open-device system: A retrospective cohort study"],"prefix":"10.1016","volume":"281","author":[{"given":"Qizhen","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meilan","family":"Mo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiru","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sisi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"10","key":"10.1016\/j.ejogrb.2022.12.012_b0005","doi-asserted-by":"crossref","first-page":"2593","DOI":"10.1093\/humrep\/der251","article-title":"An OHSS-free clinic by segmentation of IVF treatment","volume":"26","author":"Devroey","year":"2011","journal-title":"Hum Reprod"},{"issue":"2","key":"10.1016\/j.ejogrb.2022.12.012_b0010","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.rbmo.2020.03.017","article-title":"Vitrification and the demise of fresh treatment cycles in ART","volume":"41","author":"Ahuja","year":"2020","journal-title":"Reprod Biomed Online"},{"issue":"2","key":"10.1016\/j.ejogrb.2022.12.012_b0015","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/s00404-018-4786-5","article-title":"Clinical outcomes of frozen embryo versus fresh embryo transfer following in vitro fertilization: a meta-analysis of randomized controlled trials","volume":"298","author":"Zhang","year":"2018","journal-title":"Arch Gynecol Obstet"},{"issue":"6003","key":"10.1016\/j.ejogrb.2022.12.012_b0020","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1038\/313573a0","article-title":"Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification","volume":"313","author":"Rall","year":"1985","journal-title":"Nature"},{"issue":"9","key":"10.1016\/j.ejogrb.2022.12.012_b0025","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1093\/humrep\/dez095","article-title":"Perinatal outcomes of singletons following vitrification versus slow-freezing of embryos: a multicenter cohort study using propensity score analysis","volume":"34","author":"Gu","year":"2019","journal-title":"Hum Reprod"},{"issue":"2","key":"10.1016\/j.ejogrb.2022.12.012_b0030","first-page":"139","article-title":"Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance","volume":"23","author":"Rienzi","year":"2017","journal-title":"Hum Reprod Update"},{"issue":"3","key":"10.1016\/j.ejogrb.2022.12.012_b0035","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/S1472-6483(10)60869-3","article-title":"Three years of routine vitrification of human zygotes: is it still fair to advocate slow-rate freezing?","volume":"14","author":"Al-Hasani","year":"2007","journal-title":"Reprod Biomed Online"},{"issue":"1","key":"10.1016\/j.ejogrb.2022.12.012_b0040","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.fertnstert.2008.09.084","article-title":"Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human embryos","volume":"93","author":"Riggs","year":"2010","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2022.12.012_b0045","first-page":"1","article-title":"Effect of embryo cryopreservation duration on pregnancy-related complications and birthweight after frozen-thawed embryo transfer: a retrospective cohort study","author":"Xu","year":"2021","journal-title":"J Dev Orig Health Dis"},{"issue":"3","key":"10.1016\/j.ejogrb.2022.12.012_b0050","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1002\/ijgo.13381","article-title":"Does duration of cryostorage affect survival rate, pregnancy, and neonatal outcomes? Large-scale single-center study of slush nitrogen (SN2) vitrified-warmed blastocysts","volume":"152","author":"Lee","year":"2021","journal-title":"Int J Gynaecol Obstet"},{"key":"10.1016\/j.ejogrb.2022.12.012_b0055","article-title":"The effect of storage time after vitrification on pregnancy and neonatal outcomes among 24 698 patients following the first embryo transfer cycles","author":"Li","year":"2020","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2022.12.012_b0060","article-title":"Prolonged Cryopreservation Negatively Affects Embryo Transfer Outcomes Following the Elective Freeze-All Strategy: A Multicenter Retrospective Study","volume":"12","author":"Zhang","year":"2021","journal-title":"Front Endocrinol (Lausanne)"},{"key":"10.1016\/j.ejogrb.2022.12.012_b0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.fertnstert.2022.06.003","article-title":"The association between embryo storage time and treatment success in women undergoing freeze-all embryo transfer","author":"Hu","year":"2022","journal-title":"Fertil Steril"},{"issue":"4","key":"10.1016\/j.ejogrb.2022.12.012_b0070","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.rbmo.2020.01.005","article-title":"Association between the number of top-quality blastocysts and live births after single blastocyst transfer in the first fresh or vitrified-warmed IVF\/ICSI cycle","volume":"40","author":"Xiong","year":"2020","journal-title":"Reprod Biomed Online"},{"issue":"1","key":"10.1016\/j.ejogrb.2022.12.012_b0075","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s00404-020-05928-1","article-title":"High grade trophectoderm is associated with monozygotic twinning in frozen-thawed single blastocyst transfer","volume":"304","author":"Xu","year":"2021","journal-title":"Arch Gynecol Obstet"},{"issue":"5","key":"10.1016\/j.ejogrb.2022.12.012_b0080","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.rbmo.2014.01.006","article-title":"Laser-assisted hatching improves clinical outcomes of vitrified-warmed blastocysts developed from low-grade cleavage-stage embryos: a prospective randomized study","volume":"28","author":"Wan","year":"2014","journal-title":"Reprod Biomed Online"},{"issue":"6","key":"10.1016\/j.ejogrb.2022.12.012_b0085","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/S0015-0282(00)00518-5","article-title":"Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer","volume":"73","author":"Gardner","year":"2000","journal-title":"Fertil Steril"},{"issue":"3","key":"10.1016\/j.ejogrb.2022.12.012_b0090","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s00404-019-05236-3","article-title":"Clinical outcomes after transfer of blastocysts derived from frozen-thawed cleavage embryos: a retrospective propensity-matched cohort study","volume":"300","author":"Xiong","year":"2019","journal-title":"Arch Gynecol Obstet"},{"issue":"2","key":"10.1016\/j.ejogrb.2022.12.012_b0095","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.rbmo.2017.10.111","article-title":"Artificial blastocoel collapse of human blastocysts before vitrification and its effect on re-expansion after warming - a prospective observational study using time-lapse microscopy","volume":"36","author":"Kovacic","year":"2018","journal-title":"Reprod Biomed Online"},{"issue":"2","key":"10.1016\/j.ejogrb.2022.12.012_b0100","first-page":"170","article-title":"Expansion and herniation: evaluation of the best pregnancy rate predictor after quarter laser assisted hatching in frozen blastocyst transfers","volume":"24","author":"Brogliato","year":"2020","journal-title":"JBRA Assist Reprod"},{"issue":"10","key":"10.1016\/j.ejogrb.2022.12.012_b0105","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1007\/s43032-021-00538-x","article-title":"Optimal Endometrial Preparation Protocols for Frozen-thawed Embryo Transfer Cycles by Maternal Age","volume":"28","author":"Zheng","year":"2021","journal-title":"Reprod Sci"},{"key":"10.1016\/j.ejogrb.2022.12.012_b0110","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3389\/fendo.2020.00067","article-title":"The Future of Cryopreservation in Assisted Reproductive Technologies","volume":"11","author":"Bosch","year":"2020","journal-title":"Front Endocrinol (Lausanne)"},{"key":"10.1016\/j.ejogrb.2022.12.012_b0115","article-title":"Effect of embryo vitrification on the expression of brain tissue proteins in mouse offspring","volume":"1\u20135","author":"Zhu","year":"2020","journal-title":"Gynecol Endocrinol"},{"issue":"1","key":"10.1016\/j.ejogrb.2022.12.012_b0120","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s40659-018-0176-5","article-title":"Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis","volume":"51","author":"Turathum","year":"2018","journal-title":"Biol Res"},{"issue":"5","key":"10.1016\/j.ejogrb.2022.12.012_b0125","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/j.1439-0531.2005.00585.x","article-title":"Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants","volume":"40","author":"Rajaei","year":"2005","journal-title":"Reprod Domest Anim"},{"issue":"4","key":"10.1016\/j.ejogrb.2022.12.012_b0130","first-page":"254","article-title":"Chromosome abnormalities and viability of vitrified eight-cell mouse embryos at presence of two different cryoprotectants at different storage durations","volume":"14","author":"Zarei Moradi","year":"2013","journal-title":"Cell J"},{"issue":"4","key":"10.1016\/j.ejogrb.2022.12.012_b0135","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1016\/j.theriogenology.2011.03.018","article-title":"Does storage time in LN2 influence survival and pregnancy outcome of vitrified rabbit embryos?","volume":"76","author":"Lavara","year":"2011","journal-title":"Theriogenology"},{"issue":"5","key":"10.1016\/j.ejogrb.2022.12.012_b0140","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1016\/j.fertnstert.2008.02.126","article-title":"Long-term liquid nitrogen vapor storage of mouse embryos cryopreserved using vitrification or slow cooling","volume":"91","author":"Eum","year":"2009","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2022.12.012_b0145","article-title":"Long-term cryopreservation and frozen embryo transfer do not impact clinical and neonatal outcomes: a retrospective cohort study of slow-frozen early-cleavage human embryos","volume":"1\u20137","author":"Shi","year":"2022","journal-title":"Zygote"},{"issue":"1","key":"10.1016\/j.ejogrb.2022.12.012_b0150","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0015-0282(16)59298-X","article-title":"Factors influencing the success rate of human embryo freezing in an in vitro fertilization and embryo transfer program","volume":"48","author":"Testart","year":"1987","journal-title":"Fertil Steril"},{"issue":"11","key":"10.1016\/j.ejogrb.2022.12.012_b0155","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1093\/humrep\/det361","article-title":"The time aspect in storing vitrified blastocysts: its impact on survival rate, implantation potential and babies born","volume":"28","author":"Wirleitner","year":"2013","journal-title":"Hum Reprod"},{"issue":"6","key":"10.1016\/j.ejogrb.2022.12.012_b0160","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.rbmo.2018.03.008","article-title":"Cryostorage duration does not affect pregnancy and neonatal outcomes: a retrospective single-centre cohort study of vitrified-warmed blastocysts","volume":"36","author":"Ueno","year":"2018","journal-title":"Reprod Biomed Online"},{"issue":"2","key":"10.1016\/j.ejogrb.2022.12.012_b0165","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.rbmo.2021.09.027","article-title":"Clinical, obstetric and perinatal outcomes after vitrified-warmed euploid blastocyst transfer are independent of cryo-storage duration","volume":"44","author":"Cimadomo","year":"2022","journal-title":"Reprod Biomed Online"},{"issue":"4","key":"10.1016\/j.ejogrb.2022.12.012_b0170","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1007\/s10815-022-02405-3","article-title":"The effect of extended cryo-storage following vitrification on embryo competence: a systematic review and meta-analysis","volume":"39","author":"Canosa","year":"2022","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.ejogrb.2022.12.012_b0175","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2021.724853","article-title":"The Impact of Embryo Storage Time on Pregnancy and Perinatal Outcomes and the Time Limit of Vitrification: A Retrospective Cohort Study","volume":"12","author":"Cui","year":"2021","journal-title":"Front Endocrinol (Lausanne)"},{"issue":"3","key":"10.1016\/j.ejogrb.2022.12.012_b0180","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.rbmo.2010.11.004","article-title":"Effects of two vitrification protocols on the developmental potential of human mature oocytes","volume":"22","author":"Paffoni","year":"2011","journal-title":"Reprod Biomed Online"},{"issue":"1","key":"10.1016\/j.ejogrb.2022.12.012_b0185","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1186\/s12958-018-0440-0","article-title":"Open versus closed vitrification system of human oocytes and embryos: a systematic review and meta-analysis of embryologic and clinical outcomes","volume":"16","author":"Cai","year":"2018","journal-title":"Reproductive biology and endocrinology : RB&E"},{"issue":"10","key":"10.1016\/j.ejogrb.2022.12.012_b0190","first-page":"843","article-title":"Duration of storage does not influence pregnancy outcome in cryopreserved human embryos","volume":"11","author":"Aflatoonian","year":"2013","journal-title":"Iran J Reprod Med"},{"issue":"4","key":"10.1016\/j.ejogrb.2022.12.012_b0195","article-title":"Experimental Evidence Reveals Both Cross-Infection and Cross-Contamination Risk of Embryo Storage in Liquid Nitrogen Biobanks","volume":"10","author":"Marin","year":"2020","journal-title":"Animals (Basel)"},{"issue":"3","key":"10.1016\/j.ejogrb.2022.12.012_b0200","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1007\/s10815-021-02062-y","article-title":"High-security closed devices are efficient and safe to protect human oocytes from potential risk of viral contamination during vitrification and storage especially in the COVID-19 pandemic","volume":"38","author":"Porcu","year":"2021","journal-title":"J Assist Reprod Genet"},{"issue":"8","key":"10.1016\/j.ejogrb.2022.12.012_b0205","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1093\/humrep\/deac101","article-title":"Fertility intentions to have a second or third child under China's three-child policy: a national cross-sectional study","volume":"37","author":"Jing","year":"2022","journal-title":"Hum Reprod"}],"container-title":["European Journal of Obstetrics &amp; Gynecology and Reproductive Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301211522006248?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301211522006248?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T05:59:09Z","timestamp":1759557549000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0301211522006248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2]]},"references-count":41,"alternative-id":["S0301211522006248"],"URL":"https:\/\/doi.org\/10.1016\/j.ejogrb.2022.12.012","relation":{},"ISSN":["0301-2115"],"issn-type":[{"value":"0301-2115","type":"print"}],"subject":[],"published":{"date-parts":[[2023,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Prolong cryopreservation duration negatively affects pregnancy outcomes of vitrified-warmed blastocyst transfers using an open-device system: A retrospective cohort study","name":"articletitle","label":"Article Title"},{"value":"European Journal of Obstetrics & Gynecology and Reproductive Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejogrb.2022.12.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}