{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:10:31Z","timestamp":1793211031601,"version":"4.5.0"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","ejog.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Obstetrics &amp; Gynecology and Reproductive Biology"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.ejogrb.2026.115195","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:08:04Z","timestamp":1778861284000},"page":"115195","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Use of ejaculated versus testicular sperm for assisted reproduction outcomes in patients with oligozoospermia in the absence of sperm DNA fragmentation testing"],"prefix":"10.1016","volume":"323","author":[{"given":"Jo\u00e3o Roberto","family":"Paladino Jr.","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamilto Akihissa","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejogrb.2026.115195_b0005","doi-asserted-by":"crossref","unstructured":"World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: WHO; 2010.","DOI":"10.1038\/aja.2008.57"},{"key":"10.1016\/j.ejogrb.2026.115195_b0010","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1055\/s-0029-1202300","article-title":"Semen analysis and sperm function assays: what do they mean?","volume":"27","author":"Sigman","year":"2009","journal-title":"Semin Reprod Med"},{"key":"10.1016\/j.ejogrb.2026.115195_b0015","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/j.fertnstert.2019.03.005","article-title":"Limits of current male fertility testing","volume":"111","author":"Oehninger","year":"2019","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2026.115195_b0020","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/j.fertnstert.2006.02.111","article-title":"Reactive oxygen species as an independent marker of male factor infertility","volume":"86","author":"Agarwal","year":"2006","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2026.115195_b0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4103\/aja.aja_7_17","article-title":"Use of testicular sperm for intracytoplasmic sperm injection in men with high sperm DNA fragmentation: a SWOT analysis","volume":"20","author":"Esteves","year":"2018","journal-title":"Asian J Androl"},{"key":"10.1016\/j.ejogrb.2026.115195_b0030","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1016\/j.fertnstert.2015.08.028","article-title":"Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated and testicular sperm","volume":"104","author":"Esteves","year":"2015","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2026.115195_b0035","doi-asserted-by":"crossref","first-page":"9601","DOI":"10.1073\/pnas.0506468103","article-title":"Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm","volume":"103","author":"Wyrobek","year":"2006","journal-title":"PNAS"},{"key":"10.1016\/j.ejogrb.2026.115195_b0040","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1016\/j.fertnstert.2016.02.013","article-title":"Use of testicular versus ejaculated sperm for intracytoplasmic sperm injection among men with cryptozoospermia: a meta-analysis","volume":"105","author":"Abhyankar","year":"2016","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2026.115195_b0045","doi-asserted-by":"crossref","DOI":"10.1136\/bmj.c332","article-title":"CONSORT 2010 statement: updated guidelines for reporting parallel group randomised trials","volume":"340","author":"Schulz","year":"2010","journal-title":"BMJ"},{"key":"10.1016\/j.ejogrb.2026.115195_b0050","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1093\/humrep\/der092","article-title":"ESHRE consensus on the definition of poor response to ovarian stimulation for in vitro fertilization: the Bologna criteria","volume":"26","author":"Ferraretti","year":"2011","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2026.115195_b0055","first-page":"1371","article-title":"Randomized phase II clinical trials","volume":"69","author":"Simon","year":"1985","journal-title":"Cancer Treat Rep"},{"key":"10.1016\/j.ejogrb.2026.115195_b0060","doi-asserted-by":"crossref","first-page":"103","DOI":"10.4055\/cios.2014.6.1.103","article-title":"How to do random allocation (randomization)","volume":"6","author":"Kim","year":"2014","journal-title":"Clin Orthop Surg"},{"issue":"4","key":"10.1016\/j.ejogrb.2026.115195_b0065","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.fertnstert.2021.05.087","article-title":"Testicular sperm extraction vs. ejaculated sperm use for nonazoospermic male factor infertility","volume":"116","author":"Kendall Rauchfuss","year":"2021","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2026.115195_b0070","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1093\/humrep\/12.8.1688","article-title":"Physiological consequences of testicular sperm extraction","volume":"12","author":"Schlegel","year":"1997","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2026.115195_b0075","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1093\/humrep\/15.3.653","article-title":"Prospective comparative study between microsurgical and conventional testicular sperm extraction in non-obstructive azoospermia: follow-up by serial ultrasound examinations","volume":"15","author":"Amer","year":"2000","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2026.115195_b0080","doi-asserted-by":"crossref","first-page":"523","DOI":"10.5534\/wjmh.240129","article-title":"Changes in testosterone levels following surgical sperm retrieval in men with non-obstructive azoospermia: systematic review and meta-analysis","volume":"43","author":"Zohdy","year":"2025","journal-title":"World J Mens Health"},{"key":"10.1016\/j.ejogrb.2026.115195_b0085","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1093\/humrep\/deh024","article-title":"Relationship between ROS production, apoptosis, and DNA denaturation in spermatozoa from patients examined for infertility","volume":"19","author":"Moustafa","year":"2004","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2026.115195_b0090","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1093\/humrep\/den321","article-title":"Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysis","volume":"23","author":"Zini","year":"2008","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2026.115195_b0095","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1093\/humrep\/deh590","article-title":"Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa","volume":"20","author":"Greco","year":"2005","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2026.115195_b0100","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1016\/j.fertnstert.2015.08.008","article-title":"Higher pregnancy rates using testicular sperm in men with severe oligospermia","volume":"104","author":"Mehta","year":"2015","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2026.115195_b0105","doi-asserted-by":"crossref","first-page":"178","DOI":"10.23736\/S0031-0808.18.03534-6","article-title":"Testicular sperm for intracytoplasmic sperm injection in non-azoospermic men: a paradigm shift","volume":"61","author":"Lopes","year":"2019","journal-title":"Panminerva Med"},{"key":"10.1016\/j.ejogrb.2026.115195_b0110","doi-asserted-by":"crossref","first-page":"2908","DOI":"10.1093\/humrep\/des261","article-title":"The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis","volume":"27","author":"Robinson","year":"2012","journal-title":"Hum Reprod"},{"key":"10.1016\/j.ejogrb.2026.115195_b0115","doi-asserted-by":"crossref","DOI":"10.1177\/2058915815594454","article-title":"Unpacking the mysteries of sperm DNA fragmentation","volume":"4","author":"Gos\u00e1lvez","year":"2015","journal-title":"J Reprod Biotechnol Fertil"},{"key":"10.1016\/j.ejogrb.2026.115195_b0120","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.fertnstert.2010.05.055","article-title":"The effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality","volume":"95","author":"Meseguer","year":"2011","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2026.115195_b0125","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.fertnstert.2015.01.014","article-title":"Effect of sperm DNA fragmentation on the clinical outcomes for in vitro fertilization and intracytoplasmic sperm injection in women with different ovarian reserves","volume":"103","author":"Jin","year":"2015","journal-title":"Fertil Steril"},{"key":"10.1016\/j.ejogrb.2026.115195_b0130","unstructured":"Practice Committee of the American Society for Reproductive Medicine. Guidance on the limits to the number of embryos to transfer: a committee opinion. Fertil Steril. 2021;116:10\u201324."},{"key":"10.1016\/j.ejogrb.2026.115195_b0135","article-title":"Effect of day-3 and day-5\/6 embryo quality on the live birth rate and neonatal outcome after single vitrified embryo transfer: a retrospective cohort study","volume":"12","author":"Abuzeid","year":"2021","journal-title":"Front Endocrinol (Lausanne)"}],"container-title":["European Journal of Obstetrics &amp; Gynecology and Reproductive Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301211526002630?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301211526002630?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:45:47Z","timestamp":1787921147000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0301211526002630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":27,"alternative-id":["S0301211526002630"],"URL":"https:\/\/doi.org\/10.1016\/j.ejogrb.2026.115195","relation":{},"ISSN":["0301-2115"],"issn-type":[{"value":"0301-2115","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Use of ejaculated versus testicular sperm for assisted reproduction outcomes in patients with oligozoospermia in the absence of sperm DNA fragmentation testing","name":"articletitle","label":"Article Title"},{"value":"European Journal of Obstetrics & Gynecology and Reproductive Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejogrb.2026.115195","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"115195"}}