{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T02:54:54Z","timestamp":1783392894978,"version":"3.54.6"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Protistology"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.ejop.2019.125640","type":"journal-article","created":{"date-parts":[[2019,9,28]],"date-time":"2019-09-28T13:50:30Z","timestamp":1569678630000},"page":"125640","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["A comparative study of the membrane proteins from Naegleria species: A 23-kDa protein participates in the virulence of Naegleria fowleri"],"prefix":"10.1016","volume":"72","author":[{"given":"Nadia","family":"Flores-Huerta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"S\u00e1nchez-Monroy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario A.","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Serrano-Luna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mineko","family":"Shibayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejop.2019.125640_bib0005","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1006\/expr.1994.1023","article-title":"Naegleria fowleri: characterization of a secreted histolytic cysteine protease","volume":"78","author":"Aldape","year":"1994","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.ejop.2019.125640_bib0010","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1046\/j.1365-2958.1999.01476.x","article-title":"Antisense inhibition of expression of the light subunit (35kDa) of the Gal\/GalNac lectin complex inhibits Entamoeba histolytica virulence","volume":"33","author":"Ankri","year":"1999","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.ejop.2019.125640_bib0015","first-page":"1","article-title":"Structural prediction and mutational analysis of Rv3906c gene of Mycobacterium tuberculosis H(37)Rv to determine its essentiality in survival","volume":"2018","author":"Beg","year":"2018","journal-title":"Adv. Bioinf."},{"key":"10.1016\/j.ejop.2019.125640_bib0020","doi-asserted-by":"crossref","first-page":"3615","DOI":"10.1007\/s00436-013-3549-2","article-title":"Naegleria fowleri glycoconjugates with residues of alpha-d-mannose are involved in adherence of trophozoites to mouse nasal mucosa","volume":"112","author":"Carrasco-Y\u00e9pez","year":"2013","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ejop.2019.125640_bib0025","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/path.1711000402","article-title":"Description of a Naegleria sp. isolated from two cases of primary amoebic meningo-encephalitis, and of the experimental pathological changes induced by it","volume":"100","author":"Carter","year":"1970","journal-title":"J. Pathol."},{"key":"10.1016\/j.ejop.2019.125640_bib0030","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jhin.2014.05.001","article-title":"Free-living amoebae: what part do they play in healthcare-associated infections?","volume":"87","author":"Cateau","year":"2014","journal-title":"J. Hosp. Infect."},{"key":"10.1016\/j.ejop.2019.125640_bib0035","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.parint.2008.01.006","article-title":"Characterization of brain inflammation during primary amoebic meningoencephalitis","volume":"57","author":"Cervantes-Sandoval","year":"2008","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ejop.2019.125640_bib0040","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1007\/s00436-010-1727-z","article-title":"Differences between Naegleria fowleri and Naegleria gruberi in expression of mannose and fucose glycoconjugates","volume":"106","author":"Cervantes-Sandoval","year":"2010","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ejop.2019.125640_bib0045","first-page":"433","article-title":"Posttranslational modification of proteins","volume":"5","author":"Green","year":"2010","journal-title":"Compr. Nat. Prod. II Chem. Biol."},{"key":"10.1016\/j.ejop.2019.125640_bib0050","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1016\/j.meegid.2011.07.023","article-title":"Origin and evolution of the worldwide distributed pathogenic amoeboflagellate Naegleria fowleri","volume":"11","author":"De Jonckheere","year":"2011","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.ejop.2019.125640_bib0055","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.exppara.2014.07.011","article-title":"What do we know by now about the genus Naegleria?","volume":"145","author":"De Jonckheere","year":"2014","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.ejop.2019.125640_bib0060","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S1383-5769(97)00032-9","article-title":"Comparison of adherence, cytotoxicity, and Gal\/GalNAc lectin gene structure in Entamoeba histolytica and Entamoeba dispar","volume":"46","author":"Dodson","year":"1997","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ejop.2019.125640_bib0065","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1136\/bmj.2.5464.734-a","article-title":"Acute pyogenic meningitis probably due to Acanthamoeba sp.: a preliminary report","volume":"2","author":"Fowler","year":"1965","journal-title":"Br. Med. J."},{"key":"10.1016\/j.ejop.2019.125640_bib0070","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.cell.2010.01.032","article-title":"The genome of Naegleria gruberi illuminates early eukaryotic versatility","volume":"140","author":"Fritz-Laylin","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.ejop.2019.125640_bib0075","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.exppara.2007.05.007","article-title":"Cell surface differences of Naegleria fowleri and Naegleria lovaniensis exposed with surface markers","volume":"117","author":"Gonz\u00e1lez-Robles","year":"2007","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.ejop.2019.125640_bib0080","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s00436-004-1158-9","article-title":"The involvement of an integrin-like protein and protein kinase C in amoebic adhesion to fibronectin and amoebic cytotoxicity","volume":"94","author":"Han","year":"2004","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ejop.2019.125640_bib0085","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1099\/mic.0.055020-0","article-title":"Pathogenic Naegleria fowleri and non-pathogenic Naegleria lovaniensis exhibit differential adhesion to, and invasion of, extracellular matrix proteins","volume":"158","author":"Jamerson","year":"2012","journal-title":"Microbiology"},{"key":"10.1016\/j.ejop.2019.125640_bib0090","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1099\/mic.0.000428","article-title":"Identification of Naegleria fowleri proteins linked to primary amoebic meningoencephalitis","volume":"163","author":"Jamerson","year":"2017","journal-title":"Microbiology"},{"key":"10.1016\/j.ejop.2019.125640_bib0095","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1645\/0022-3395(2000)086[0050:ALMSOT]2.0.CO;2","article-title":"A light microscopy study of the migration of Naegleria fowleri from the nasal submucosa to the central nervous system during the early stage of primary amebic meningoencephalitis in mice","volume":"86","author":"Jarolim","year":"2000","journal-title":"J. Parasitol."},{"key":"10.1016\/j.ejop.2019.125640_bib0100","first-page":"309","article-title":"A century of research on the amoeboflagellate genus Naegleria","volume":"41","author":"Johan","year":"2002","journal-title":"Acta Protozool."},{"key":"10.1016\/j.ejop.2019.125640_bib0105","first-page":"187","article-title":"Virulence of Naegleria fowleri affected by axenic cultivation and passage in mice","volume":"40","author":"John","year":"1993","journal-title":"Folia Parasitol."},{"key":"10.1016\/j.ejop.2019.125640_bib0110","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ejop.2019.125640_bib0115","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1099\/mic.0.000537","article-title":"Expression of matrix metalloproteinases in Naegleria fowleri and their role in invasion of the central nervous system","volume":"163","author":"Lam","year":"2017","journal-title":"Microbiology"},{"key":"10.1016\/j.ejop.2019.125640_bib0120","doi-asserted-by":"crossref","first-page":"018","DOI":"10.1186\/s12864-018-4994-1","article-title":"The genome of Naegleria lovaniensis, the basis for a comparative approach to unravel pathogenicity factors of the human pathogenic amoeba N. fowleri","volume":"19","author":"Liechti","year":"2018","journal-title":"BMC Genomics"},{"key":"10.1016\/j.ejop.2019.125640_bib0125","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.ejop.2019.125640_bib0130","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1128\/IAI.74.1.340-351.2006","article-title":"Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species: potential implications for amebic pathogenesis","volume":"74","author":"MacFarlane","year":"2006","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ejop.2019.125640_bib0135","doi-asserted-by":"crossref","first-page":"781","DOI":"10.2217\/fmb-2016-0230","article-title":"Nf-GH, a glycosidase secreted by Naegleria fowleri, causes mucin degradation: an in vitro and in vivo study","volume":"12","author":"Mart\u00ednez-Castillo","year":"2017","journal-title":"Future Microbiol."},{"key":"10.1016\/j.ejop.2019.125640_bib0140","first-page":"121","article-title":"Experimental Naegleria meningoencephalitis in mice. Penetration of the olfactory mucosal epithelium by Naegleria and pathologic changes produced: a light and electron microscope study","volume":"29","author":"Mart\u00ednez","year":"1973","journal-title":"Lab. Invest."},{"key":"10.1016\/j.ejop.2019.125640_bib0145","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.4161\/cc.28401","article-title":"Partial inhibition of Cdk1 in G2 phase overrides the SAC and decouples mitotic events","volume":"13","author":"McCloy","year":"2014","journal-title":"Cell Cycle"},{"key":"10.1016\/j.ejop.2019.125640_bib0150","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/jeu.12178","article-title":"Detection of biomarkers of pathogenic Naegleria fowleri through mass spectrometry and proteomics","volume":"62","author":"Moura","year":"2015","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.ejop.2019.125640_bib0155","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/j.1550-7408.2001.tb00208.x","article-title":"Isolation of a unique membrane protein from Naegleria fowleri","volume":"48","author":"Reveiller","year":"2001","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.ejop.2019.125640_bib0160","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00436-004-1177-6","article-title":"Immunohistochemical characterization of the initial stages of Naegleria fowleri meningoencephalitis in mice","volume":"94","author":"Rojas-Hern\u00e1ndez","year":"2004","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ejop.2019.125640_bib0165","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1093\/nar\/gks372","article-title":"COFACTOR: an accurate comparative algorithm for structure-based protein function annotation","volume":"40","author":"Roy","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ejop.2019.125640_bib0170","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/prot.21078","article-title":"Prediction of continuous B-cell epitopes in an antigen using recurrent neural network","volume":"65","author":"Saha","year":"2006","journal-title":"Proteins"},{"key":"10.1016\/j.ejop.2019.125640_bib0175","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.ijpara.2004.06.004","article-title":"Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals","volume":"34","author":"Schuster","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ejop.2019.125640_bib0180","first-page":"35","article-title":"Post-translational modifications and their biological functions: proteomic analysis and systematic approaches","volume":"37","author":"Seo","year":"2004","journal-title":"J. Biochem. Mol. Biol."},{"key":"10.1016\/j.ejop.2019.125640_bib0185","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/j.1550-7408.2007.00280.x","article-title":"A biochemical comparison of proteases from pathogenic Naegleria fowleri and non-pathogenic Naegleria gruberi","volume":"54","author":"Serrano-Luna","year":"2007","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.ejop.2019.125640_bib0190","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.actatropica.2016.09.009","article-title":"Biology and pathogenesis of Naegleria fowleri","volume":"164","author":"Siddiqui","year":"2016","journal-title":"Acta Trop."},{"key":"10.1016\/j.ejop.2019.125640_bib0195","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0020-7519(80)90064-8","article-title":"Naegleria lovaniensis new species: isolation and identification of six thermophilic strains of a new species found in association with Naegleria fowleri","volume":"10","author":"Stevens","year":"1980","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ejop.2019.125640_bib0200","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.patbio.2012.03.002","article-title":"Pathogenic free-living amoebae: epidemiology and clinical review","volume":"60","author":"Trabelsi","year":"2012","journal-title":"Pathol. Biol."},{"key":"10.1016\/j.ejop.2019.125640_bib0205","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/jeu.12152","article-title":"Identification of peptidases in highly pathogenic vs. weakly pathogenic Naegleria fowleri amebae","volume":"62","author":"Vyas","year":"2015","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.ejop.2019.125640_bib0210","doi-asserted-by":"crossref","first-page":"872","DOI":"10.2307\/3279897","article-title":"Changes in the virulence of Naegleria fowleri maintained in vitro","volume":"63","author":"Wong","year":"1977","journal-title":"J. Parasitol."},{"key":"10.1016\/j.ejop.2019.125640_bib0215","doi-asserted-by":"crossref","first-page":"W174","DOI":"10.1093\/nar\/gkv342","article-title":"I-TASSER server: new development for protein structure and function predictions","volume":"43","author":"Yang","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ejop.2019.125640_bib0220","doi-asserted-by":"crossref","first-page":"W291","DOI":"10.1093\/nar\/gkx366","article-title":"COFACTOR: improved protein function prediction by combining structure, sequence and protein\u2013protein interaction information","volume":"45","author":"Zhang","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ejop.2019.125640_bib0225","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1111\/j.1550-7408.2003.tb00617.x","article-title":"Genetic variations in the internal transcribed spacer and mitochondrial small subunit rRNA gene of Naegleria spp","volume":"50","author":"Zhou","year":"2003","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.ejop.2019.125640_bib0230","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1186\/1471-2164-15-496","article-title":"Genome-wide identification of pathogenicity factors of the free-living amoeba Naegleria fowleri","volume":"15","author":"Zysset-Burri","year":"2014","journal-title":"BMC Genomics"}],"container-title":["European Journal of Protistology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S093247391930077X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S093247391930077X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T14:59:09Z","timestamp":1760540349000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S093247391930077X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":46,"alternative-id":["S093247391930077X"],"URL":"https:\/\/doi.org\/10.1016\/j.ejop.2019.125640","relation":{},"ISSN":["0932-4739"],"issn-type":[{"value":"0932-4739","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A comparative study of the membrane proteins from Naegleria species: A 23-kDa protein participates in the virulence of Naegleria fowleri","name":"articletitle","label":"Article Title"},{"value":"European Journal of Protistology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejop.2019.125640","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"125640"}}