{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T06:42:22Z","timestamp":1782542542858,"version":"3.54.5"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100009010","name":"Henan Agricultural University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Protistology"],"published-print":{"date-parts":[[2021,6]]},"DOI":"10.1016\/j.ejop.2021.125796","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T03:38:47Z","timestamp":1619149127000},"page":"125796","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Molecular identification and subtyping of Blastocystis sp. in hospital patients in Central China"],"prefix":"10.1016","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6417-0901","authenticated-orcid":false,"given":"Junqiang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiju","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Robiul","family":"Karim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianli","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqin","family":"Chao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hairong","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejop.2021.125796_b0005","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.actatropica.2012.12.011","article-title":"Variable geographic distribution of Blastocystis subtypes and its potential implications","volume":"126","author":"Alfellani","year":"2013","journal-title":"Acta Trop."},{"key":"10.1016\/j.ejop.2021.125796_b0010","doi-asserted-by":"crossref","first-page":"25255","DOI":"10.1038\/srep25255","article-title":"Colonization with the enteric protozoa Blastocystis is associated with increased diversity of human gut bacterial microbiota","volume":"6","author":"Audebert","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ejop.2021.125796_b0015","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1007\/s10096-019-03793-8","article-title":"Blastocystis, urticaria, and skin disorders: review of the current evidences","volume":"39","author":"Bahrami","year":"2020","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ejop.2021.125796_b0025","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1756-3305-1-40","article-title":"Oh my aching gut: irritable bowel syndrome, Blastocystis, and asymptomatic infection","volume":"1","author":"Boorom","year":"2008","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ejop.2021.125796_b0030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0169659","article-title":"Molecular Epidemiology of Blastocystis sp. in Various Animal Groups from Two French Zoos and Evaluation of Potential Zoonotic Risk","volume":"12","author":"Cian","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.ejop.2021.125796_b0035","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1016\/j.apjtm.2016.07.018","article-title":"Blastocystis in ulcerative colitis patients: Genetic diversity and analysis of laboratory findings","volume":"9","author":"Coskun","year":"2016","journal-title":"Asian Pac. J. Trop. Med."},{"key":"10.1016\/j.ejop.2021.125796_b0040","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1051\/parasite\/2019042","article-title":"Epidemiology of Blastocystis sp. infection in China: a systematic review","volume":"26","author":"Deng","year":"2019","journal-title":"Parasite."},{"key":"10.1016\/j.ejop.2021.125796_b0045","article-title":"Lower prevalence of Blastocystis sp. infections in HIV positive compared to HIV negative adults in Ghana","volume":"14","author":"Di Cristanziano","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.ejop.2021.125796_b0050","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1186\/s12879-019-4537-7","article-title":"Molecular characterization of Blastocystis subtypes in HIV-positive patients and evaluation of risk factors for colonization","volume":"19","author":"Fontanelli","year":"2019","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.ejop.2021.125796_b0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.parepi.2020.e00134","article-title":"Occurrence of Blastocystis-subtypes in patients from Italy revealed association of ST3 with a healthy gut microbiota","volume":"9","author":"Gabrielli","year":"2020","journal-title":"Parasite Epidemiol. Control"},{"key":"10.1016\/j.ejop.2021.125796_b0060","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.8554","article-title":"Molecular detection and genotyping of intestinal protozoa from different biogeographical regions of Colombia","volume":"8","author":"Higuera","year":"2020","journal-title":"PeerJ"},{"key":"10.1016\/j.ejop.2021.125796_b0065","first-page":"63","article-title":"Subtypes distribution and frequency of Blastocystis sp. isolated from diarrheic and non-diarrheic patients","volume":"12","author":"Jalallou","year":"2017","journal-title":"Iran J. Parasitol."},{"key":"10.1016\/j.ejop.2021.125796_b0070","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1007\/s00436-011-2626-7","article-title":"Blastocystis infection is associated with irritable bowel syndrome in a Mexican patient population","volume":"110","author":"Jimenez-Gonzalez","year":"2012","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ejop.2021.125796_b0075","first-page":"479","article-title":"Prevalence and risk factors of Blastocystis hominis infection in inpatients in Jiangjin District, Chongqing City","volume":"31","author":"Kang","year":"2019","journal-title":"Chin. J. Schi. Contl."},{"key":"10.1016\/j.ejop.2021.125796_b0080","doi-asserted-by":"crossref","first-page":"321","DOI":"10.3347\/kjp.2020.58.3.321","article-title":"Molecular detection and subtyping of human Blastocystis and the clinical implications: comparisons between diarrheal and non-diarrheal groups in Korean populations","volume":"58","author":"Kim","year":"2020","journal-title":"Korean J. Parasitol."},{"key":"10.1016\/j.ejop.2021.125796_b0085","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.ijppaw.2019.11.003","article-title":"Molecular characterization of Blastocystis sp. in captive wildlife in Bangladesh National Zoo: Non-human primates with high prevalence and zoonotic significance","volume":"10","author":"Li","year":"2019","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"10.1016\/j.ejop.2021.125796_b0090","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s00436-007-0727-0","article-title":"Cross-sectional surveys and subtype classification of human Blastocystis isolates from four epidemiological settings in China","volume":"102","author":"Li","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ejop.2021.125796_b0095","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.parint.2007.06.001","article-title":"Molecular epidemiology of human Blastocystis in a village in Yunnan province, China","volume":"56","author":"Li","year":"2007","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ejop.2021.125796_b0100","first-page":"348","article-title":"Diagnosis and treatment of two cases of Blastocystis hominis","volume":"28","author":"Li","year":"1990","journal-title":"Chinese J. Pediatr."},{"key":"10.1016\/j.ejop.2021.125796_b0105","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.actatropica.2015.05.001","article-title":"Prevalence and risk factors of intestinal protozoan and helminth infections among pulmonary tuberculosis patients without HIV infection in a rural county in P. R. China","volume":"149","author":"Li","year":"2015","journal-title":"Acta Trop."},{"key":"10.1016\/j.ejop.2021.125796_b0110","doi-asserted-by":"crossref","DOI":"10.1111\/tbed.13644","article-title":"Molecular prevalence and subtypes of Blastocystis sp. in primates in northern China","author":"Ma","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.ejop.2021.125796_b0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.parepi.2019.e00124","article-title":"Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, S\u00e3o Paulo, Brazil","volume":"7","author":"Melo","year":"2019","journal-title":"Parasite Epidemiol. Control"},{"key":"10.1016\/j.ejop.2021.125796_b0120","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1111\/j.1445-5994.2011.02626.x","article-title":"Blastocystis subtypes in symptomatic and asymptomatic family members and pets and response to therapy","volume":"42","author":"Nagel","year":"2012","journal-title":"Intern. Med. J."},{"key":"10.1016\/j.ejop.2021.125796_b0125","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/s40168-016-0191-0","article-title":"Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients","volume":"4","author":"Nagel","year":"2016","journal-title":"Microbiome"},{"key":"10.1016\/j.ejop.2021.125796_b0130","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s12639-019-01177-5","article-title":"Molecular epidemiology of Blastocystis spp. in children referred to Qods hospital in northwest of Iran","volume":"44","author":"Niaraki","year":"2020","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.ejop.2021.125796_b0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.parepi.2020.e00139","article-title":"Prevalence and subtype distribution of Blastocystis sp. infecting children from a rural community in Panama","volume":"9","author":"Perea","year":"2020","journal-title":"Parasite Epidemiol. Control."},{"key":"10.1016\/j.ejop.2021.125796_b0140","doi-asserted-by":"crossref","unstructured":"Qi, M., Wei, Z., Zhang, Y., Zhang, Q., Li, J., Zhang, L., Wang, R., 2020. Genetic diversity of Blastocystis in kindergarten children in southern Xinjiang, China. Parasit. Vectors 13, 15.","DOI":"10.1186\/s13071-020-3890-0"},{"key":"10.1016\/j.ejop.2021.125796_b0145","doi-asserted-by":"crossref","first-page":"308","DOI":"10.4269\/ajtmh.2011.10-0447","article-title":"Comparison of microscopy, culture, and conventional polymerase chain reaction for detection of Blastocystis sp. in clinical stool samples","volume":"84","author":"Roberts","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.ejop.2021.125796_b0150","first-page":"1","article-title":"Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey","volume":"28","author":"Sarzhanov","year":"2020","journal-title":"Parasitology."},{"key":"10.1016\/j.ejop.2021.125796_b0155","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.protis.2005.12.001","article-title":"DNA barcoding of Blastocystis","volume":"157","author":"Scicluna","year":"2006","journal-title":"Protist"},{"key":"10.1016\/j.ejop.2021.125796_b0160","doi-asserted-by":"crossref","first-page":"90","DOI":"10.2478\/s11686-019-00131-y","article-title":"Prevalence and molecular subtyping of Blastocystis from patients with irritable bowel syndrome, inflammatory bowel disease and chronic Urticaria in Iran","volume":"65","author":"Shirvani","year":"2020","journal-title":"Acta Parasitol."},{"key":"10.1016\/j.ejop.2021.125796_b0165","article-title":"Subtypes of Blastocystis sp. isolated in fecal samples from transplant candidates in S\u00e3o Paulo, Brazil","volume":"8","author":"Silva","year":"2019","journal-title":"Parasite Epidemiol. Control."},{"key":"10.1016\/j.ejop.2021.125796_b0170","doi-asserted-by":"crossref","first-page":"411","DOI":"10.26444\/aaem\/81315","article-title":"Genetic diversity and pathogenicity of Blastocystis","volume":"25","author":"Skotarczak","year":"2018","journal-title":"Ann. Agric. Environ. Med."},{"key":"10.1016\/j.ejop.2021.125796_b0175","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.parint.2016.05.015","article-title":"Current status of Blastocystis: A personal view","volume":"65","author":"Stensvold","year":"2016","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ejop.2021.125796_b0180","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.pt.2019.12.009","article-title":"Pre-empting Pandora\u2019s box: Blastocystis subtypes revisited","volume":"36","author":"Stensvold","year":"2020","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ejop.2021.125796_b0185","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.pt.2019.12.008","article-title":"Blastocystis","volume":"36","author":"Stensvold","year":"2020","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ejop.2021.125796_b0195","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.cimid.2019.06.005","article-title":"Blastocystis subtype 1 (allele 4); Predominant subtype among tuberculosis patients in Iran","volume":"65","author":"Taghipour","year":"2019","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ejop.2021.125796_b0200","first-page":"167","article-title":"Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis","volume":"1","author":"Wawrzyniak","year":"2013","journal-title":"Ther. Adv. Infect. Dis."},{"key":"10.1016\/j.ejop.2021.125796_b0210","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/s40249-019-0596-7","article-title":"Risk factors for Blastocystis infection in HIV\/AIDS patients with highly active antiretroviral therapy in Southwest China","volume":"8","author":"Zhang","year":"2019","journal-title":"Infect. Dis. Poverty"},{"key":"10.1016\/j.ejop.2021.125796_b0215","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.3389\/fmicb.2017.01835","article-title":"Genotyping of Enterocytozoon bieneusi and subtyping of Blastocystis in cancer patients: relationship to diarrhea and assessment of zoonotic transmission","volume":"8","author":"Zhang","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ejop.2021.125796_b0220","first-page":"607","article-title":"Survey and Analysis of epidemic status of principal human parasitosis in ecological region of Huaiyang hills of Henan Province in 2015","volume":"29","author":"Zhang","year":"2017","journal-title":"Chin. J. Schi. Contl."},{"key":"10.1016\/j.ejop.2021.125796_b0225","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1007\/s00436-020-06761-w","article-title":"Prevalence and subtype diversity of Blastocystis in human and nonhuman primates in North China","volume":"119","author":"Zhu","year":"2020","journal-title":"Parasitol. Res."}],"container-title":["European Journal of Protistology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0932473921000316?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0932473921000316?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T15:00:33Z","timestamp":1760540433000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0932473921000316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6]]},"references-count":42,"alternative-id":["S0932473921000316"],"URL":"https:\/\/doi.org\/10.1016\/j.ejop.2021.125796","relation":{},"ISSN":["0932-4739"],"issn-type":[{"value":"0932-4739","type":"print"}],"subject":[],"published":{"date-parts":[[2021,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular identification and subtyping of Blastocystis sp. in hospital patients in Central China","name":"articletitle","label":"Article Title"},{"value":"European Journal of Protistology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejop.2021.125796","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"125796"}}