{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:48:47Z","timestamp":1792442927657,"version":"4.5.0"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pharmaceutics and Biopharmaceutics"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.ejpb.2020.04.010","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T02:36:28Z","timestamp":1587609388000},"page":"189-198","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":59,"special_numbering":"C","title":["Lipid-polymer hybrid nanoparticles carrying linezolid improve treatment of methicillin-resistant Staphylococcus aureus (MRSA) harbored inside bone cells and biofilms"],"prefix":"10.1016","volume":"151","author":[{"given":"Pengbo","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina A.","family":"Buttaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui Yi","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ngoc T.","family":"Tran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho Lun","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9431","key":"10.1016\/j.ejpb.2020.04.010_b0005","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0140-6736(04)16727-5","volume":"364","author":"Lew","year":"2004","journal-title":"The Lancet"},{"issue":"9","key":"10.1016\/j.ejpb.2020.04.010_b0010","first-page":"1027","article-title":"Diagnosis and management of osteomyelitis","volume":"84","author":"Hatzenbuehler","year":"2011","journal-title":"Am. Family Phys."},{"issue":"3","key":"10.1016\/j.ejpb.2020.04.010_b0015","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1093\/cid\/cir842","article-title":"Systemic antibiotic therapy for chronic osteomyelitis in adults","volume":"54","author":"Spellberg","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ejpb.2020.04.010_b0020","doi-asserted-by":"crossref","unstructured":"H.M. Kremers, M.E. Nwojo, J.E. Ransom, C.M. Wood-Wentz, L.J. Melton III, P.M. Huddleston III, Trends in the epidemiology of osteomyelitis: a population-based study, 1969 to 2009, J. Bone Joint Surgery Am. 97(10) (2015) 837.","DOI":"10.2106\/JBJS.N.01350"},{"issue":"6","key":"10.1016\/j.ejpb.2020.04.010_b0025","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1128\/AAC.37.6.1214","article-title":"Oral rifampin plus ofloxacin for treatment of Staphylococcus-infected orthopedic implants","volume":"37","author":"Drancourt","year":"1993","journal-title":"Antimicrob. Agents Chemother."},{"issue":"2","key":"10.1016\/j.ejpb.2020.04.010_b0030","doi-asserted-by":"crossref","first-page":"85","DOI":"10.2165\/00003495-199958002-00017","article-title":"Use of quinolones in osteomyelitis and infected orthopaedic prosthesis","volume":"58","author":"Lew","year":"1999","journal-title":"Drugs"},{"issue":"7","key":"10.1016\/j.ejpb.2020.04.010_b0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002158","article-title":"Selection of resistant bacteria at very low antibiotic concentrations","volume":"7","author":"Gullberg","year":"2011","journal-title":"PLoS Pathog."},{"issue":"1","key":"10.1016\/j.ejpb.2020.04.010_b0040","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1086\/595011","article-title":"Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America","volume":"48","author":"Boucher","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ejpb.2020.04.010_b0045","doi-asserted-by":"crossref","unstructured":"L.B. Rice, Federal funding for the study of antimicrobial resistance in nosocomial pathogens: no ESKAPE (2008).","DOI":"10.1086\/533452"},{"key":"10.1016\/j.ejpb.2020.04.010_b0050","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1136\/pmj.76.898.479","article-title":"Pathophysiology of chronic bacterial osteomyelitis: why do antibiotics fail so often?","volume":"76","author":"Ciampolini","year":"2000","journal-title":"Postgrad. Med. J."},{"issue":"3","key":"10.1016\/j.ejpb.2020.04.010_b0055","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens6030031","article-title":"Staphylococcus aureus Regulator Sigma B is important to develop chronic infections in hematogenous murine osteomyelitis model","volume":"6","author":"Tuchscherr","year":"2017","journal-title":"Pathogens"},{"issue":"2","key":"10.1016\/j.ejpb.2020.04.010_b0060","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1093\/jac\/dkv371","article-title":"Staphylococcus aureus develops increased resistance to antibiotics by forming dynamic small colony variants during chronic osteomyelitis","volume":"71","author":"Tuchscherr","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.ejpb.2020.04.010_b0065","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/j.1574-695X.2007.00357.x","article-title":"Osteomyelitis and the role of biofilms in chronic infection","volume":"52","author":"Brady","year":"2008","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.ejpb.2020.04.010_b0070","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.colsurfa.2011.08.035","article-title":"The roles of lipid in anti-biofilm efficacy of lipid\u2013polymer hybrid nanoparticles encapsulating antibiotics","volume":"389","author":"Cheow","year":"2011","journal-title":"Colloids Surf. A"},{"key":"10.1016\/j.ejpb.2020.04.010_b0075","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1007\/s11095-010-0142-6","article-title":"Antibacterial efficacy of inhalable levofloxacin-loaded polymeric nanoparticles against E. coli biofilm cells: the effect of antibiotic release profile","volume":"27","author":"Cheow","year":"2010","journal-title":"Pharm. Res."},{"key":"10.1016\/j.ejpb.2020.04.010_b0080","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ijpharm.2009.02.001","article-title":"Bismuth-thiol incorporation enhances biological activities of liposomal tobramycin against bacterial biofilm and quorum sensing molecules production by Pseudomonas aeruginosa","volume":"373","author":"Halwani","year":"2009","journal-title":"Int. J. Pharm."},{"issue":"7","key":"10.1016\/j.ejpb.2020.04.010_b0085","doi-asserted-by":"crossref","first-page":"941","DOI":"10.2174\/1567201815666180228162949","article-title":"Therapeutic nanotechnology for bone infection treatment - State of the art","volume":"15","author":"Guo","year":"2018","journal-title":"Curr. Drug Deliv."},{"key":"10.1016\/j.ejpb.2020.04.010_b0090","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.micpath.2016.11.016","article-title":"Approach to osteomyelitis treatment with antibiotic loaded PMMA","volume":"102","author":"Wentao","year":"2017","journal-title":"Microb. Pathog."},{"issue":"2","key":"10.1016\/j.ejpb.2020.04.010_b0095","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1089\/sur.2010.050","article-title":"Experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus treated with a polylactide carrier releasing linezolid","volume":"12","author":"Tsiolis","year":"2011","journal-title":"Surg. Infect. (Larchmt)"},{"key":"10.1016\/j.ejpb.2020.04.010_b0100","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1081\/LPR-120016195","article-title":"The application of confocal microscopy to the study of liposome adsorption onto bacterial biofilms","volume":"12","author":"Ahmed","year":"2002","journal-title":"J. Liposome Res."},{"key":"10.1016\/j.ejpb.2020.04.010_b0105","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1163\/156856200744408","article-title":"The use of immunoliposomes for specific delivery of antimicrobial agents to oral bacteria immobilized on polystyrene","volume":"11","author":"Robinson","year":"2000","journal-title":"J. Biomat. Sci. Polym. Ed"},{"key":"10.1016\/j.ejpb.2020.04.010_b0110","doi-asserted-by":"crossref","first-page":"2694","DOI":"10.1128\/AAC.00235-13","article-title":"Efficacy and safety of liposomal clarithromycin and its effect on Pseudomonas aeruginosa virulence factors","volume":"57","author":"Alhajlan","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"issue":"5","key":"10.1016\/j.ejpb.2020.04.010_b0115","doi-asserted-by":"crossref","first-page":"ofz181","DOI":"10.1093\/ofid\/ofz181","article-title":"The history of antibiotic treatment of osteomyelitis","author":"Cort\u00e9s-Penfield","year":"2019","journal-title":"Open Forum Infect. Dis. 6"},{"key":"10.1016\/j.ejpb.2020.04.010_b0120","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.msec.2016.04.034","article-title":"Loading of atorvastatin and linezolid in \u03b2-cyclodextrin-conjugated cadmium selenide\/silica nanoparticles: a spectroscopic study","volume":"65","author":"Antony","year":"2016","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"issue":"1\u20132","key":"10.1016\/j.ejpb.2020.04.010_b0125","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.ijpharm.2013.11.048","article-title":"Enhanced cellular uptake by \u201cpharmaceutically oriented devices\u201d of new simplified analogs of Linezolid with antimicrobial activity","volume":"461","author":"Parisi","year":"2014","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.ejpb.2020.04.010_b0130","doi-asserted-by":"crossref","first-page":"3199","DOI":"10.2147\/DDDT.S172199","article-title":"Synergistic combination therapy of lung cancer using paclitaxel- and triptolide-coloaded lipid-polymer hybrid nanoparticles","volume":"12","author":"Liu","year":"2018","journal-title":"Drug Des. Devel. Ther."},{"key":"10.1016\/j.ejpb.2020.04.010_b0135","unstructured":"Malvern Panalytical. Common Terms Used In Dynamic Light Scattering \u2013 AzoM. https:\/\/www.azom.com\/article.aspx?ArticleID=9924 (retrieved on April 11, 2020)."},{"key":"10.1016\/j.ejpb.2020.04.010_b0140","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.ijbiomac.2017.08.151","article-title":"Lactoferrin promotes MC3T3-E1 osteoblast cells proliferation via MAPK signaling pathways","volume":"107","author":"Liu","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"issue":"7","key":"10.1016\/j.ejpb.2020.04.010_b0145","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.ajic.2005.06.006","article-title":"Emergence of community-associated methicillin-resistant Staphylococcus aureus USA 300 clone as a cause of health care-associated infections among patients with prosthetic joint infections","volume":"33","author":"Kourbatova","year":"2005","journal-title":"Am. J. Infect. Control"},{"key":"10.1016\/j.ejpb.2020.04.010_b0150","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1046\/j.1365-2672.2000.01017.x","article-title":"Susceptibility testing: accurate and reproducible minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) values","volume":"88","author":"Lambert","year":"2000","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.ejpb.2020.04.010_b0155","first-page":"2437","article-title":"Microtiter dish biofilm formation assay","volume":"47","author":"O'Toole","year":"2011","journal-title":"Journal of visualized experiments"},{"key":"10.1016\/j.ejpb.2020.04.010_b0160","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ijpharm.2010.10.037","article-title":"Elaborations on the Higuchi model for drug delivery","volume":"418","author":"Paul","year":"2011","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.ejpb.2020.04.010_b0165","doi-asserted-by":"crossref","unstructured":"G. Rollin, X. Tan, F. Tros, M. Dupuis, X. Nassif, A. Charbit, M. Coureuil, Intracellular Survival of Staphylococcus aureus in endothelial cells: a matter of growth or persistence, Front. Microbiol. 8 (2017) 1354 https:\/\/doi.org\/10.3389\/fmicb.2017.01354 e2017.","DOI":"10.3389\/fmicb.2017.01354"},{"key":"10.1016\/j.ejpb.2020.04.010_b0170","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02447-19","article-title":"Staphylococcus aureus infects osteoclasts and replicates intracellularly","volume":"10","author":"Krauss","year":"2019","journal-title":"mBio"},{"key":"10.1016\/j.ejpb.2020.04.010_b0175","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.2147\/DDDT.S164515","article-title":"Linezolid: a review of its properties, function, and use in critical care","volume":"12","author":"Hashemian","year":"2018","journal-title":"Drug Des. Devel. Ther."},{"key":"10.1016\/j.ejpb.2020.04.010_b0180","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.2147\/IJN.S198353","article-title":"Lipid-polymer hybrid nanoparticles as a next-generation drug delivery platform: state of the art, emerging technologies, and perspectives","volume":"14","author":"Mukherjee","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.ejpb.2020.04.010_b0185","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.ijpharm.2006.11.061","article-title":"Haloperidol-loaded PLGA nanoparticles: systematic study of particle size and drug content","volume":"336","author":"Budhian","year":"2007","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.ejpb.2020.04.010_b0190","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.1021\/nn800275r","article-title":"Self-assembled lipid\u2013polymer hybrid nanoparticles: a robust drug delivery platform","volume":"2","author":"Zhang","year":"2008","journal-title":"ACS Nano"},{"key":"10.1016\/j.ejpb.2020.04.010_b0195","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1016\/j.biomaterials.2008.12.013","article-title":"PLGA-lecithin-PEG core-shell nanoparticles for controlled drug delivery","volume":"30","author":"Chan","year":"2009","journal-title":"Biomaterials"},{"key":"10.1016\/j.ejpb.2020.04.010_b0200","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.ijpharm.2010.02.008","article-title":"Transferrin-conjugated lipid-coated PLGA nanoparticles for targeted delivery of aromatase inhibitor 7alpha-APTADD to breast cancer cells","volume":"390","author":"Zheng","year":"2010","journal-title":"Int. J. Pharm."},{"issue":"1\u20132","key":"10.1016\/j.ejpb.2020.04.010_b0205","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.ijpharm.2013.11.048","article-title":"Linezolid penetration into osteo-articular tissues","volume":"461","author":"Parisi","year":"2014","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.ejpb.2020.04.010_b0210","doi-asserted-by":"crossref","first-page":"5879","DOI":"10.2147\/IJN.S123437","article-title":"Nanomedicine applications in the treatment of breast cancer: current state of the art","volume":"12","author":"Wu","year":"2017","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.ejpb.2020.04.010_b0215","unstructured":"Zyvox (linezolid) label information, Pfizer (2015) http:\/\/labeling.pfizer.com\/ShowLabeling.aspx?id=649#section-11.3."},{"issue":"5","key":"10.1016\/j.ejpb.2020.04.010_b0220","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1093\/jac\/dkf207","article-title":"Linezolid penetration into osteo-articular tissues","volume":"50","author":"Rana","year":"2002","journal-title":"J. Antimicrob. Chemother."},{"issue":"4","key":"10.1016\/j.ejpb.2020.04.010_b0225","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.ijantimicag.2008.08.029","article-title":"Comparison of biofilm-associated cell survival following in vitro exposure of meticillin-resistant Staphylococcus aureus biofilms to the antibiotics clindamycin, daptomycin, linezolid, tigecycline and vancomycin","volume":"33","author":"Smith","year":"2009","journal-title":"Int. J. Antimicrob. Agents"},{"issue":"4","key":"10.1016\/j.ejpb.2020.04.010_b0230","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1021\/acs.molpharmaceut.8b00011","article-title":"PEGylation of tobramycin improves mucus penetration and antimicrobial activity against Pseudomonas aeruginosa biofilms in vitro","volume":"15","author":"Bahamondez-Canas","year":"2018","journal-title":"Mol. Pharm."}],"container-title":["European Journal of Pharmaceutics and Biopharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0939641120301041?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0939641120301041?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T16:38:24Z","timestamp":1760546304000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0939641120301041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":46,"alternative-id":["S0939641120301041"],"URL":"https:\/\/doi.org\/10.1016\/j.ejpb.2020.04.010","relation":{},"ISSN":["0939-6411"],"issn-type":[{"value":"0939-6411","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Lipid-polymer hybrid nanoparticles carrying linezolid improve treatment of methicillin-resistant Staphylococcus aureus (MRSA) harbored inside bone cells and biofilms","name":"articletitle","label":"Article Title"},{"value":"European Journal of Pharmaceutics and Biopharmaceutics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejpb.2020.04.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}