{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:13:55Z","timestamp":1790957635809,"version":"4.1.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/21029-3"],"award-info":[{"award-number":["2019\/21029-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["166541\/2017-6"],"award-info":[{"award-number":["166541\/2017-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","award":["16\/2551-0000467-6"],"award-info":[{"award-number":["16\/2551-0000467-6"]}],"id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pharmaceutics and Biopharmaceutics"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1016\/j.ejpb.2020.12.016","type":"journal-article","created":{"date-parts":[[2021,1,8]],"date-time":"2021-01-08T21:25:50Z","timestamp":1610141150000},"page":"99-107","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"special_numbering":"C","title":["Docosahexaenoic acid nanoencapsulated with anti-PECAM-1 as co-therapy for atherosclerosis regression"],"prefix":"10.1016","volume":"159","author":[{"given":"Matheus","family":"de Castro Le\u00e3o","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Raffin Pohlmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"de Cristo Soares Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Helena Poliselli Farsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayara","family":"Klimuk Uchiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koiti","family":"Araki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Sandri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Stanis\u00e7uaski Guterres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inar","family":"Alves Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejpb.2020.12.016_b0005","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.colsurfb.2018.02.024","article-title":"Albumin-covered lipid nanocapsules exhibit enhanced uptake performance by breast-tumor cells","volume":"165","author":"Galisteo-Gonz\u00e1lez","year":"2018","journal-title":"Colloids Surf. B. Biointerf."},{"key":"10.1016\/j.ejpb.2020.12.016_b0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep44998","article-title":"Biodegradable Polymeric Nanocapsules Prevent Cardiotoxicity of Anti-Trypanosomal Lychnopholide","volume":"7","author":"Branquinho","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ejpb.2020.12.016_b0015","doi-asserted-by":"crossref","first-page":"653","DOI":"10.2147\/IJN.S149458","article-title":"Bioreducible nanocapsules for folic acid-assisted targeting and effective tumor-specific chemotherapy","volume":"13","author":"Yi","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.ejpb.2020.12.016_b0020","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3390\/nano8010024","article-title":"Enhanced and selective antiproliferative activity of methotrexate-funcionalized-nanocapsules to human breast cancer cells (MCF-7)","volume":"4","author":"Oliveira","year":"2018","journal-title":"Nanomat"},{"key":"10.1016\/j.ejpb.2020.12.016_b0025","first-page":"E34","article-title":"Surface-Modified nanocarriers for nose-to-brain delivery: From bioadhesion to targeting","volume":"10","author":"Sonvico","year":"2018","journal-title":"Pharm."},{"key":"10.1016\/j.ejpb.2020.12.016_b0030","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.numecd.2017.11.001","article-title":"Hypertriglyceridemia and \u00f4mega-3 fatty acids: Their often overlooked role in cardiovascular disease prevention","volume":"28","author":"Arca","year":"2018","journal-title":"Nutr. Metab. Cardiovasc. Dis."},{"key":"10.1016\/j.ejpb.2020.12.016_b0035","first-page":"277","article-title":"Evolution of omega-3 fatty acid therapy and current and future role in the management of dyslipidemia","volume":"36","author":"Benes","year":"2018","journal-title":"Cardio. Clinics"},{"key":"10.1016\/j.ejpb.2020.12.016_b0040","first-page":"469","article-title":"Marine omega-3 fatty acids and inflammatory processes: effects, mechanisms and clinical relevance","volume":"2015","author":"Calder","year":"1851","journal-title":"Biochimica et Biophys. Acta (BBA)"},{"issue":"9","key":"10.1016\/j.ejpb.2020.12.016_b0045","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/j.jnutbio.2009.12.004","article-title":"Mechanisms underlying the cardioprotective effects of omega-3 polyunsaturated fatty acids","volume":"21","author":"Adkins","year":"2010","journal-title":"J. Nutritional Biochem."},{"key":"10.1016\/j.ejpb.2020.12.016_b0050","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.freeradbiomed.2016.12.005","article-title":"Non-enzymatic oxidized metabolite of DHA, 4 (RS)-4-F4t-neuroprostane protects the heart against reperfusion injury","volume":"102","author":"Roy","year":"2017","journal-title":"Free Radical Biol. Med."},{"key":"10.1016\/j.ejpb.2020.12.016_b0055","doi-asserted-by":"crossref","first-page":"2392","DOI":"10.1016\/j.jacc.2018.03.002","article-title":"Anti-inflammatory therapy with canakinumab for the prevention and management of diabetes","volume":"71","author":"Everett","year":"2018","journal-title":"J. Am. Coll. Cardiol."},{"issue":"1","key":"10.1016\/j.ejpb.2020.12.016_b0060","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0021-9150(86)90027-4","article-title":"Primary culture of endothelial cells from atherosclerotic human aorta: Part 1 Identification, morphological and ultrastructural characteristics of two endothelial cell subpopulations","volume":"59","author":"Antonov","year":"1986","journal-title":"Atherosclerosis"},{"key":"10.1016\/j.ejpb.2020.12.016_b0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.addr.2020.06.013","article-title":"Targeting drug delivery in the vascular system: Focus on endothelium","author":"Glassman","year":"2020","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"1","key":"10.1016\/j.ejpb.2020.12.016_b0070","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1096\/fj.10-169789","article-title":"PECAM-targeted delivery of SOD inhibits endothelial inflammatory response","volume":"25","author":"Shuvaev","year":"2011","journal-title":"FASEB J."},{"key":"10.1016\/j.ejpb.2020.12.016_b0075","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.addr.2018.06.023","article-title":"Unintended effects of drug carriers: Big issues of small particles","volume":"130","author":"Parhiz","year":"2018","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.ejpb.2020.12.016_b0080","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1182\/blood-2007-07-103002","article-title":"Prophylactic thrombolysis by thrombin-activated latent prourokinase targeted to PECAM-1 in the pulmonary vasculature","volume":"111","author":"Ding","year":"2008","journal-title":"Blood"},{"key":"10.1016\/j.ejpb.2020.12.016_b0085","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.jconrel.2015.09.055","article-title":"Targeted endothelial nanomedicine for common acute pathological conditions","volume":"219","author":"Shuvaev","year":"2015","journal-title":"J. Control. Release"},{"issue":"1","key":"10.1016\/j.ejpb.2020.12.016_b0090","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-20027-7","article-title":"Vascular endothelial effects of collaborative binding to platelet\/endothelial cell adhesion molecule-1 (PECAM-1)","volume":"8","author":"Kiseleva","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ejpb.2020.12.016_b0095","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1242\/jcs.082271","article-title":"Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity","volume":"124","author":"Privratsky","year":"2011","journal-title":"J Cell Sci."},{"key":"10.1016\/j.ejpb.2020.12.016_b0100","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1242\/dmm.000547","article-title":"PECAM-1 is a critical mediator of atherosclerosis","volume":"1","author":"Stevens","year":"2008","journal-title":"Dis. Models Mech."},{"key":"10.1016\/j.ejpb.2020.12.016_b0105","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0077002","article-title":"Anti-Inflammatory Effect of Targeted Delivery of SOD to Endothelium: Mechanism, Synergism with NO Donors and Protective Effects In Vitro and In Vivo","volume":"8","author":"Shuvaev","year":"2013","journal-title":"PLoS ONE"},{"issue":"4","key":"10.1016\/j.ejpb.2020.12.016_b0110","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/nbt806","article-title":"Immunotargeting of catalase to the pulmonary endothelium alleviates oxidative stress and reduces acute lung transplantation injury","volume":"21","author":"Kozower","year":"2003","journal-title":"Nat. Biotechnol."},{"issue":"2","key":"10.1016\/j.ejpb.2020.12.016_b0115","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.jconrel.2012.08.031","article-title":"Antioxidant protection by PECAM-targeted delivery of a novel NADPH-oxidase inhibitor to the endothelium in vitro and in vivo","volume":"163","author":"Hood","year":"2012","journal-title":"J. Control. Release"},{"key":"10.1016\/j.ejpb.2020.12.016_b0120","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1146\/annurev-pharmtox-011613-140002","article-title":"Nanocarriers for vascular delivery of anti-inflammatory agents","volume":"54","author":"Howard","year":"2014","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"issue":"2","key":"10.1016\/j.ejpb.2020.12.016_b0125","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.biomaterials.2007.09.023","article-title":"Endothelial targeting of semi-permeable polymer nanocarriers for enzyme therapies","volume":"29","author":"Dziubla","year":"2008","journal-title":"Biomaterials"},{"key":"10.1016\/j.ejpb.2020.12.016_b0130","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ab.2005.04.037","article-title":"Direct methylation from mouse plasma and from liver and brain homogenates","volume":"343","author":"Shirai","year":"2005","journal-title":"Anal Biochem."},{"key":"10.1016\/j.ejpb.2020.12.016_b0135","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1093\/jaoac\/77.2.421","article-title":"Rapid, Sensitive, Iron-based spectrophotometric methods for determination of peroxide values of food lipids","volume":"77","author":"Shanta","year":"1994","journal-title":"J. AOAC Int."},{"key":"10.1016\/j.ejpb.2020.12.016_b0140","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/S0009-9120(00)00177-6","article-title":"Total plasma malondialdehyde levels in 16 Taiwanese college students determined by various thiobarbituric acid tests and an improved high-performance liquid chromatography-based method","volume":"33","author":"Hong","year":"2000","journal-title":"Clin Biochem."},{"key":"10.1016\/j.ejpb.2020.12.016_b0145","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.chroma.2004.07.051","article-title":"Simple reversed-phase liquid chromatography method for determination of tocopherols in edible plant oils","volume":"1048","author":"Gliszczy\u0144ska-\u015awig\u0142o","year":"2004","journal-title":"J. Chromatogr. A."},{"key":"10.1016\/j.ejpb.2020.12.016_b0150","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1080\/107175400455119","article-title":"Influence of benzyl benzoate as oil core on the physicochemical properties of spray-dried powders from polymeric nanocapsules containing indomethacin","volume":"7","author":"Guterres","year":"2000","journal-title":"Drug Deliv."},{"key":"10.1016\/j.ejpb.2020.12.016_b0155","doi-asserted-by":"crossref","first-page":"2975","DOI":"10.1007\/s11095-014-1392-5","article-title":"New strategy to surface functionalization of polymeric nanoparticles: one-pot synthesis of scFv anti-LDL(-)-funcionalized nanocapsules","volume":"31","author":"Bender","year":"2014","journal-title":"Pharm. Res."},{"key":"10.1016\/j.ejpb.2020.12.016_b0160","doi-asserted-by":"crossref","unstructured":"W. Strober, Trypan blue exclusion test of cell viability, Current protocols in immunology, 21.1 (1997), pp. A-3B. 10.1002\/0471142735.ima03bs21.","DOI":"10.1002\/0471142735.ima03bs21"},{"key":"10.1016\/j.ejpb.2020.12.016_b0165","unstructured":"Codex Alimentarius Commission. Codex alimentarius: fats, oils and related products., 8. FAO (2001). ISBN 92-5-104682-4."},{"key":"10.1016\/j.ejpb.2020.12.016_b0170","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.lwt.2018.11.044","article-title":"Oxidation products from omega-3 and omega-6 fatty acids during a simulated shelf life of edible oils","volume":"101","author":"Nogueira","year":"2019","journal-title":"LWT"},{"key":"10.1016\/j.ejpb.2020.12.016_b0175","first-page":"2","article-title":"Controlled Drug Delivery with Nanoparticles: Current possibilities and future trends","volume":"41","author":"Couvreur","year":"1995","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.ejpb.2020.12.016_b0180","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1111\/j.1600-0846.2012.00631.x","article-title":"Vitamin K1\u2013loaded lipid-core nanocapsules: physicochemical characterization and in vitro skin permeation","volume":"19","author":"Silva","year":"2013","journal-title":"Skin Res. Tech."},{"key":"10.1016\/j.ejpb.2020.12.016_b0185","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.ejpb.2016.07.002","article-title":"A nanoformulation containing a scFv reactive to electronegative LDL inhibits atherosclerosis in LDL receptor knockout mice","volume":"107","author":"Cavalcante","year":"2016","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.ejpb.2020.12.016_b0190","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.jddst.2015.10.015","article-title":"Radar charts based on particle sizing as an approach to establish the fingerprints of polymeric nanoparticles in aqueous formulations","volume":"30","author":"Bianchin","year":"2015","journal-title":"J. Drug Delivery Sci. Technol."},{"key":"10.1016\/j.ejpb.2020.12.016_b0195","doi-asserted-by":"crossref","first-page":"6646","DOI":"10.1039\/c2sm25754h","article-title":"Lipid-core nanocapsules: mechanism of self-assembly, control of size and loading capacity","volume":"8","author":"Jornada","year":"2012","journal-title":"Soft Matter."},{"key":"10.1016\/j.ejpb.2020.12.016_b0200","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1590\/S0100-40422012000100024","article-title":"Desenvolvimento de nanoc\u00e1psulas de poli-e-caprolactona contendo o herbicida atrazina","volume":"35","author":"Souza","year":"2012","journal-title":"Quim. Nova"},{"key":"10.1016\/j.ejpb.2020.12.016_b0205","doi-asserted-by":"crossref","first-page":"81051","DOI":"10.1371\/journal.pone.0081051","article-title":"Binding, transcytosis and biodistribution of anti-PECAM-1 iron oxide nanoparticles for brain-targeted delivery","volume":"8","author":"Dan","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.ejpb.2020.12.016_b0210","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.mam.2018.03.003","article-title":"Biological activities of non-enzymatic oxygenated metabolites of polyunsaturated fatty acids (NEO-PUFAs) derived from EPA and DHA: New anti-arrhythmic compounds?","volume":"64","author":"Galano","year":"2018","journal-title":"Mol. Aspects Med."},{"issue":"1","key":"10.1016\/j.ejpb.2020.12.016_b0215","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1186\/s13046-015-0273-z","article-title":"Polymeric nanocapsules prevent oxidation of core-loaded molecules: evidence based on the effects of docosahexaenoic acid and neuroprostane on breast cancer cells proliferation","volume":"34","author":"Roy","year":"2015","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"10.1016\/j.ejpb.2020.12.016_b0220","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jnutbio.2018.07.014","article-title":"Comparison between red wine and isolated trans-resveratrol on the prevention and regression of atherosclerosis in LDLr (\u2212\/\u2212) mice","volume":"61","author":"Chassot","year":"2018","journal-title":"J. Nutritional Biochem."},{"key":"10.1016\/j.ejpb.2020.12.016_b0225","doi-asserted-by":"crossref","unstructured":"M. S. Nogueira,M. C. Kessuane,A. A. B. Lobo Ladd,F. V. Lobo Ladd, Effect of long-term ingestion of weakly oxidised flaxseed oil on biomarkers of oxidative stress in LDL-receptor knockout mice, 116 (2016), pp. 258-269. 10.1017\/S0007114516001513.","DOI":"10.1017\/S0007114516001513"},{"key":"10.1016\/j.ejpb.2020.12.016_b0230","doi-asserted-by":"crossref","unstructured":"J. Roy, J-Y. Le Guennec, Cardioprotective effects of omega 3 fatty acids: origin of the variability, J Muscle Res Cell Motil, 38 (1), pp.25-30. 10.1007\/s10974-016-9459-z.","DOI":"10.1007\/s10974-016-9459-z"},{"key":"10.1016\/j.ejpb.2020.12.016_b0235","doi-asserted-by":"crossref","DOI":"10.1016\/j.pharmthera.2019.107436","article-title":"Therapeutic strategy for atherosclerosis based on bone-vascular axis hypothesis","volume":"206","author":"Kim","year":"2020","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.ejpb.2020.12.016_b0240","doi-asserted-by":"crossref","first-page":"16","DOI":"10.3389\/fcvm.2019.00016","article-title":"Targeting residual inflammatory risk: a shifting paradigm for atherosclerotic disease","volume":"6","author":"Aday","year":"2019","journal-title":"Front. Cardiovas. Med."},{"issue":"7","key":"10.1016\/j.ejpb.2020.12.016_b0245","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.jacbts.2020.03.015","article-title":"Innate Immune Receptors, Key Actors in Cardiovascular Diseases","volume":"5","author":"Ja\u00e9n","year":"2020","journal-title":"JACC Basic Translation Sci."}],"container-title":["European Journal of Pharmaceutics and Biopharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0939641120303714?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0939641120303714?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T23:41:55Z","timestamp":1777592515000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0939641120303714"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":49,"alternative-id":["S0939641120303714"],"URL":"https:\/\/doi.org\/10.1016\/j.ejpb.2020.12.016","relation":{},"ISSN":["0939-6411"],"issn-type":[{"value":"0939-6411","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Docosahexaenoic acid nanoencapsulated with anti-PECAM-1 as co-therapy for atherosclerosis regression","name":"articletitle","label":"Article Title"},{"value":"European Journal of Pharmaceutics and Biopharmaceutics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejpb.2020.12.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}