{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:01:31Z","timestamp":1758819691304},"reference-count":41,"publisher":"Elsevier BV","issue":"1-3","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Pharmacology"],"published-print":{"date-parts":[[2003,12]]},"DOI":"10.1016\/j.ejphar.2003.10.008","type":"journal-article","created":{"date-parts":[[2003,11,24]],"date-time":"2003-11-24T14:09:05Z","timestamp":1069682945000},"page":"101-109","source":"Crossref","is-referenced-by-count":22,"title":["Muscarinic receptor subtypes in the human colon: lack of evidence for atypical subtypes"],"prefix":"10.1016","volume":"482","author":[{"given":"Kylie J","family":"Mansfield","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick J","family":"Mitchelson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate H","family":"Moore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Burcher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejphar.2003.10.008_BIB1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1111\/j.2042-7158.1990.tb06581.x","article-title":"Some differential effects of 4-diphenylacetoxy-N-(2-chloroethyl)-piperidine hydrochloride on guinea-pig atria and ileum","volume":"42","author":"Barlow","year":"1990","journal-title":"J. Pharm. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB2","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1139\/y95-017","article-title":"Otenzepad shows two populations of binding sites in human gastric smooth muscle","volume":"73","author":"Bellido","year":"1995","journal-title":"Can. J. Physiol. Pharm."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB3","article-title":"Expression and location of muscarinic receptor subtypes in human colon","volume":"10","author":"Burcher","year":"2003","journal-title":"Proc. Australas. Soc. Clin. Exp. Pharmacol. Toxicol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB4","doi-asserted-by":"crossref","first-page":"2473","DOI":"10.1016\/S0024-3205(01)01041-4","article-title":"Investigations into the physiological role of muscarinic M2 and M4 muscarinic and M4 receptor subtypes using receptor knockout mice","volume":"68","author":"Bymaster","year":"2001","journal-title":"Life Sci."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB5","doi-asserted-by":"crossref","first-page":"C387","DOI":"10.1152\/ajpcell.1991.261.2.C387","article-title":"Regulation of Ca(2+)-activated K+ channels by protein kinase A and phosphatase inhibitors","volume":"261","author":"Carl","year":"1991","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB6","first-page":"279","article-title":"International Union of Pharmacology: XVII. Classification of muscarinic acetylcholine receptors","volume":"50","author":"Caulfield","year":"1998","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB7","doi-asserted-by":"crossref","first-page":"3099","DOI":"10.1016\/0006-2952(73)90196-2","article-title":"Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (IC50) of an enzymatic reaction","volume":"22","author":"Cheng","year":"1973","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0922-4106(93)90002-Q","article-title":"Binding of the muscarine receptor antagonist heptane-1,7-bis(dimethyl-3\u2032-phthalimidopropyl) ammonium bromide at cholinoceptor sites","volume":"246","author":"Christopoulos","year":"1993","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB9","first-page":"G429","article-title":"Coupling of M2 muscarinic receptors to ERK MAP kinases and caldesmon phosphorylation in colonic smooth muscle","volume":"278","author":"Cook","year":"2000","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB10","first-page":"5","article-title":"Validation and statistical analysis of a computer modelling method for quantitative analysis of radioligand binding data for mixtures of pharmacological receptor subtypes","volume":"21","author":"De Lean","year":"1982","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB11","first-page":"727","article-title":"Antagonist binding profiles of five cloned human muscarinic receptor subtypes","volume":"256","author":"D\u00f6rje","year":"1991","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB12","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1016\/S0024-3205(01)01054-2","article-title":"Muscarinic receptors and gastrointestinal tract smooth muscle function","volume":"68","author":"Eglen","year":"2001","journal-title":"Life Sci."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB14","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1111\/j.1476-5381.1990.tb12983.x","article-title":"The interaction of parafluorohexahydrosiladiphenidol at muscarinic receptors in vitro","volume":"99","author":"Eglen","year":"1990","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB15","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/0165-6147(94)90047-7","article-title":"Muscarinic acetylcholine receptor subtypes in smooth muscle","volume":"15","author":"Eglen","year":"1994","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB13","first-page":"531","article-title":"Muscarinic receptor subtypes and smooth muscle function","volume":"48","author":"Eglen","year":"1996","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB16","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1016\/S0024-3205(98)00124-6","article-title":"The use of irreversible ligands to inactivate receptor subtypes: 4-DAMP mustard and muscarinic receptors in smooth muscle","volume":"62","author":"Ehlert","year":"1998","journal-title":"Life Sci."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB17","first-page":"405","article-title":"The quaternary transformation products of N-(3-chloropropyl)-4-piperidinyl diphenylacetate and N-(2-chloroethyl)-4-piperidinyl diphenylacetate (4-DAMP mustard) have differential affinity for subtypes of the muscarinic receptor","volume":"276","author":"Ehlert","year":"1996","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB18","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/S0024-3205(98)00584-0","article-title":"Contractile role of M2 and M3 muscarinic receptors in gastrointestinal smooth muscle","volume":"64","author":"Ehlert","year":"1999","journal-title":"Life Sci."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB19","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/0006-2952(93)90549-C","article-title":"Reduction in the numbers of muscarinic receptors by an endogenous protein","volume":"46","author":"Fang","year":"1993","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB20","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0014-2999(98)00214-3","article-title":"Comparison of the in vitro and in vivo profiles of tolterodine with those of subtype-selective muscarinic receptor antagonists","volume":"349","author":"Gillberg","year":"1998","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB21","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/0006-8993(92)90141-U","article-title":"A novel M2-selective muscarinic antagonist: binding characteristics and autoradiographic distribution in rat brain","volume":"582","author":"Gitler","year":"1992","journal-title":"Brain Res."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB22","doi-asserted-by":"crossref","first-page":"2413","DOI":"10.1016\/0006-2952(92)90321-9","article-title":"Muscarinic receptor subtypes in human and rat colon smooth muscle","volume":"43","author":"Gomez","year":"1992","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB23","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1111\/j.1476-5381.1995.tb16645.x","article-title":"Characterization of muscarinic receptors mediating contractions of circular and longitudinal smooth muscle of human isolated colon","volume":"115","author":"Kerr","year":"1995","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB24","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1146\/annurev.physiol.61.1.117","article-title":"The enteric nervous system and regulation of intestinal motility","volume":"61","author":"Kunze","year":"1999","journal-title":"Annu. Rev. Physiol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB25","first-page":"707","article-title":"Heterogeneity of binding of muscarinic receptor antagonists in rat brain homogenates","volume":"233","author":"Lee","year":"1985","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB26","first-page":"527","article-title":"Muscarinic receptor binding in human detrusor\u2014age related changes","volume":"22","author":"Mansfield","year":"2003","journal-title":"Neurourol. Urodyn."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB27","first-page":"663","article-title":"Binding and functional selectivity of himbacine for cloned and neuronal muscarinic receptors","volume":"263","author":"Miller","year":"1992","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB28","first-page":"1","article-title":"The kinetics of competitive ligand binding produced by the law of mass action","volume":"25","author":"Motulsky","year":"1984","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB29","doi-asserted-by":"crossref","first-page":"2351","DOI":"10.1016\/S0024-3205(99)00188-5","article-title":"Affinity profiles of various muscarinic antagonists for cloned human muscarinic acetylcholine receptor (mACh) subtypes and mAChRs in rat heart and submandibular gland","volume":"64","author":"Moriya","year":"1999","journal-title":"Life Sci."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB30","first-page":"879","article-title":"Pharmacological and molecular characterization of muscarinic receptor subtypes in human esophageal smooth muscle","volume":"295","author":"Preiksaitis","year":"2000","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB31","first-page":"269","article-title":"Contractile roles of the M2 and M3 muscarinic receptors in the guinea pig colon","volume":"284","author":"Sawyer","year":"1998","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB32","first-page":"877","article-title":"M2 and M4 receptor knockout mice: muscarinic receptor function in cardiac and smooth muscle in vitro","volume":"292","author":"Stengel","year":"2000","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB33","first-page":"718","article-title":"Conversion of N-(2-chloroethyl)-4-piperidinyl diphenylacetate (4-DAMP mustard) to an aziridinium ion and its interaction with muscarinic receptors in various tissues","volume":"41","author":"Thomas","year":"1992","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB34","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0006-2952(92)90011-7","article-title":"Inactivation of brain cortex muscarinic receptors by 4-diphenylacetoxy-1-(2-chloroethyl) piperidine mustard","volume":"44","author":"Waelbroeck","year":"1992","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB35","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/0024-3205(94)00021-J","article-title":"Pre-clinical and clinical pharmacology of selective muscarinic M3 receptor antagonists","volume":"56","author":"Wallis","year":"1995","journal-title":"Life Sci."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB36","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/S0024-3205(98)00585-2","article-title":"Muscarinic antagonists in development for disorders of smooth muscle function","volume":"64","author":"Wallis","year":"1999","journal-title":"Life Sci."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB37","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/sj.bjp.0702551","article-title":"Comparative pharmacology of recombinant human M3 and M5 muscarinic receptors expressed in CHO-K1 cells","volume":"127","author":"Watson","year":"1999","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB38","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1111\/j.1476-5381.1991.tb12161.x","article-title":"Selectivity profile of the novel muscarinic antagonist UH-AH 37 determined by the use of cloned receptors and isolated tissue preparations","volume":"102","author":"Wess","year":"1991","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB39","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0014-2999(96)00130-6","article-title":"Muscarinic receptors in developing rat colon","volume":"304","author":"Zhang","year":"1996","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB41","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1113\/jphysiol.1995.sp020791","article-title":"Effects of divalent cations on muscarinic receptor cationic current in smooth muscle from guinea-pig small intestine","volume":"486","author":"Zholos","year":"1995","journal-title":"J. Physiol."},{"key":"10.1016\/j.ejphar.2003.10.008_BIB40","first-page":"943","article-title":"Muscarinic receptors in canine colonic circular smooth muscle: I. Coexistence of M2 and M3 subtypes","volume":"40","author":"Zhang","year":"1991","journal-title":"Mol. Pharmacol."}],"container-title":["European Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014299903025263?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014299903025263?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,2,16]],"date-time":"2019-02-16T19:56:47Z","timestamp":1550347007000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014299903025263"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12]]},"references-count":41,"journal-issue":{"issue":"1-3","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["S0014299903025263"],"URL":"https:\/\/doi.org\/10.1016\/j.ejphar.2003.10.008","relation":{},"ISSN":["0014-2999"],"issn-type":[{"value":"0014-2999","type":"print"}],"subject":[],"published":{"date-parts":[[2003,12]]}}}