{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T19:37:32Z","timestamp":1767382652188,"version":"3.44.0"},"reference-count":43,"publisher":"Elsevier BV","issue":"1-3","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pharmacology"],"published-print":{"date-parts":[[2012,6]]},"DOI":"10.1016\/j.ejphar.2012.03.030","type":"journal-article","created":{"date-parts":[[2012,3,29]],"date-time":"2012-03-29T12:34:26Z","timestamp":1333024466000},"page":"146-153","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Gadolinium modulates gentamicin uptake via an endocytosis-independent pathway in HK-2 human renal proximal tubular cell line"],"prefix":"10.1016","volume":"684","author":[{"given":"Takeshi","family":"Sawada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junya","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Okada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoko","family":"Yumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikihisa","family":"Takano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejphar.2012.03.030_bb0005","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.tips.2010.11.011","article-title":"Renal glucose reabsorption inhibitors to treat diabetes","volume":"32","author":"Bailey","year":"2011","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0010","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0015","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1038\/nrm778","article-title":"Megalin and cubilin: multifunctional endocytic receptors","volume":"3","author":"Christensen","year":"2002","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0025","first-page":"349","article-title":"Segmental distribution of the endocytosis receptor gp330 in renal proximal tubules","volume":"66","author":"Christensen","year":"1995","journal-title":"Eur. J. Cell Biol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0030","doi-asserted-by":"crossref","first-page":"8472","DOI":"10.1073\/pnas.1432873100","article-title":"Loss of chloride channel ClC-5 impairs endocytosis by defective trafficking of megalin and cubilin in kidney proximal tubules","volume":"100","author":"Christensen","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0020","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1007\/s00424-009-0685-8","article-title":"Receptor-mediated endocytosis in renal proximal tubule","volume":"458","author":"Christensen","year":"2009","journal-title":"Pflugers Arch."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0035","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1161\/HYPERTENSIONAHA.110.152256","article-title":"HK-2 human renal proximal tubule cells as a model for G protein-coupled receptor kinase type 4-mediated dopamine 1 receptor uncoupling","volume":"56","author":"Gildea","year":"2010","journal-title":"Hypertension"},{"key":"10.1016\/j.ejphar.2012.03.030_bb0040","doi-asserted-by":"crossref","first-page":"F77","DOI":"10.1152\/ajprenal.00581.2009","article-title":"Ouabain stimulates Na-K-ATPase through a sodium\/hydrogen exchanger-1 (NHE-1)-dependent mechanism in human kidney proximal tubule cells","volume":"299","author":"Holthouser","year":"2010","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0045","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1016\/S0022-3565(25)11139-7","article-title":"Surface binding and intracellular uptake of gentamicin in the cultured kidney epithelial cell line (LLC-PK1)","volume":"261","author":"Hori","year":"1992","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0050","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/ki.1996.378","article-title":"Myoglobin inhibits proliferation of cultured human proximal tubular (HK-2) cells","volume":"50","author":"Iwata","year":"1996","journal-title":"Kidney Int."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0055","doi-asserted-by":"crossref","first-page":"2871","DOI":"10.1242\/jcs.023705","article-title":"TRPV4 enhances the cellular uptake of aminoglycoside antibiotics","volume":"121","author":"Karasawa","year":"2008","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0060","doi-asserted-by":"crossref","first-page":"F82","DOI":"10.1152\/ajprenal.00091.2008","article-title":"Silencing megalin and cubilin genes inhibits myeloma light chain endocytosis and ameliorates toxicity in human renal proximal tubule epithelial cells","volume":"295","author":"Li","year":"2008","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0065","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/ki.2010.337","article-title":"New insights into the mechanism of aminoglycoside nephrotoxicity: an integrative point of view","volume":"79","author":"Lopez-Novoa","year":"2011","journal-title":"Kidney Int."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0070","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1128\/AAC.43.5.1003","article-title":"Aminoglycosides: nephrotoxicity","volume":"43","author":"Mingeot-Leclercq","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0075","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1172\/JCI118176","article-title":"Evidence that epithelial glycoprotein 330\/megalin mediates uptake of polybasic drugs","volume":"96","author":"Moestrup","year":"1995","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0080","first-page":"F907","article-title":"Mechanism of ischemia-enhanced aminoglycoside binding and uptake by proximal tubule cells","volume":"264","author":"Molitoris","year":"1993","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0085","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.heares.2005.02.005","article-title":"TRPV1 regulators mediate gentamicin penetration of cultured kidney cells","volume":"204","author":"Myrdal","year":"2005","journal-title":"Hear. Res."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0090","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.heares.2005.02.002","article-title":"Cytoplasmic and intra-nuclear binding of gentamicin does not require endocytosis","volume":"204","author":"Myrdal","year":"2005","journal-title":"Hear. Res."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0095","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2133\/dmpk.19.159","article-title":"Molecular aspects of renal handling of aminoglycosides and strategies for preventing the nephrotoxicity","volume":"19","author":"Nagai","year":"2004","journal-title":"Drug Metab. Pharmacokinet."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0100","doi-asserted-by":"crossref","first-page":"F337","DOI":"10.1152\/ajprenal.2001.281.2.F337","article-title":"Role of megalin in renal handling of aminoglycosides","volume":"281","author":"Nagai","year":"2001","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0105","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jconrel.2006.01.003","article-title":"Inhibition of gentamicin binding to rat renal brush-border membrane by megalin ligands and basic peptides","volume":"112","author":"Nagai","year":"2006","journal-title":"J. Control. Release"},{"key":"10.1016\/j.ejphar.2012.03.030_bb0110","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1681\/ASN.V9101767","article-title":"Characterization of a kidney proximal tubule cell line, LLC-PK1, expressing endocytotic active megalin","volume":"9","author":"Nielsen","year":"1998","journal-title":"J. Am. Soc. Nephrol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0115","doi-asserted-by":"crossref","first-page":"4082","DOI":"10.1073\/pnas.90.9.4082","article-title":"Identification of a cell line that expresses a cell surface and a soluble form of the gp330\/receptor-associated protein (RAP) Heymann nephritis antigenic complex","volume":"90","author":"Orlando","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0120","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.trim.2010.05.003","article-title":"Sirolimus and everolimus reduce albumin endocytosis in proximal tubule cells via an angiotensin II-dependent pathway","volume":"23","author":"Oroszl\u00e1n","year":"2010","journal-title":"Transpl. Immunol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0125","doi-asserted-by":"crossref","first-page":"F301","DOI":"10.1152\/ajprenal.00471.2009","article-title":"Renoprotective effects of paricalcitol on gentamicin-induced kidney injury in rats","volume":"298","author":"Park","year":"2010","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0130","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s11010-010-0665-5","article-title":"LAT1 overexpression and function compensates downregulation of ASCT2 in an in vitro model of renal proximal tubule cell ageing","volume":"349","author":"Pinho","year":"2011","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0135","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/ki.1994.6","article-title":"HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney","volume":"45","author":"Ryan","year":"1994","journal-title":"Kidney Int."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0140","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/S0022-3565(25)24376-2","article-title":"Mechanisms of gentamicin transport in kidney epithelial cell line (LLC-PK1)","volume":"238","author":"Saito","year":"1986","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0145","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/S0022-3565(25)33203-9","article-title":"Identification of the aminoglycoside binding site in rat renal brush border membranes","volume":"222","author":"Sastrasinh","year":"1982","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0150","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/BF00455236","article-title":"Isolation of an aminoglycoside receptor from guinea pig inner ear tissues and kidney","volume":"224","author":"Schacht","year":"1979","journal-title":"Arch. Otorhinolaryngol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0155","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1074\/jbc.M109959200","article-title":"Megalin deficiency offers protection from renal aminoglycoside accumulation","volume":"277","author":"Schmitz","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0160","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s10495-007-0151-z","article-title":"Renal cell apoptosis induced by nephrotoxic drugs: cellular and molecular mechanisms and potential approaches to modulation","volume":"13","author":"Servais","year":"2008","journal-title":"Apoptosis"},{"key":"10.1016\/j.ejphar.2012.03.030_bb0165","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1007\/s10162-011-0288-x","article-title":"TRPA1-Mediated Accumulation of Aminoglycosides in Mouse Cochlear Outer Hair Cells","volume":"12","author":"Stepanyan","year":"2011","journal-title":"J. Assoc. Res. Otolaryngol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0170","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/S0022-3565(25)38990-1","article-title":"Transport of gentamicin and fluid-phase endocytosis markers in the LLC-PK1 kidney epithelial cell line","volume":"268","author":"Takano","year":"1994","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0175","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.mcn.2005.07.004","article-title":"Gadolinium activates and sensitizes the vanilloid receptor TRPV1 through the external protonation sites","volume":"30","author":"Tousova","year":"2005","journal-title":"Mol. Cell. Neurosci."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0180","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/ki.1981.50","article-title":"Gentamicin incorporation along the nephron: autoradiographic study on isolated tubules","volume":"19","author":"Vandewalle","year":"1981","journal-title":"Kidney Int."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0185","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.jconrel.2003.12.005","article-title":"Targeted prevention of renal accumulation and toxicity of gentamicin by aminoglycoside binding receptor antagonists","volume":"95","author":"Watanabe","year":"2004","journal-title":"J. Control. Release"},{"key":"10.1016\/j.ejphar.2012.03.030_bb0190","first-page":"212","article-title":"Transport of gentamicin in rat proximal tubule","volume":"48","author":"Wedeen","year":"1983","journal-title":"Lab. Invest."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0195","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1440-1797.2009.01152.x","article-title":"Urinary proteins from patients with nephrotic syndrome alters the signalling proteins regulating epithelial\u2013mesenchymal transition","volume":"15","author":"Wen","year":"2010","journal-title":"Nephrology (Carlton)"},{"key":"10.1016\/j.ejphar.2012.03.030_bb0200","doi-asserted-by":"crossref","first-page":"F10","DOI":"10.1152\/ajprenal.2001.280.1.F10","article-title":"Renal Na+\u2013glucose cotransporters","volume":"280","author":"Wright","year":"2001","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0205","doi-asserted-by":"crossref","first-page":"F1373","DOI":"10.1152\/ajprenal.00333.2007","article-title":"Gentamicin suppresses endotoxin-driven TNF-alpha production in human and mouse proximal tubule cells","volume":"293","author":"Zager","year":"2007","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0210","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1046\/j.1523-1755.2000.00314.x","article-title":"Cubilin- and megalin-mediated uptake of albumin in cultured proximal tubule cells of opossum kidney","volume":"58","author":"Zhai","year":"2000","journal-title":"Kidney Int."},{"key":"10.1016\/j.ejphar.2012.03.030_bb0215","doi-asserted-by":"crossref","first-page":"61","DOI":"10.2131\/jts.31.61","article-title":"Increased expression of heat shock protein (HSP)72 in a human proximal tubular cell line (HK-2) with gentamicin-induced injury","volume":"31","author":"Zhipeng","year":"2006","journal-title":"J. Toxicol. Sci."}],"container-title":["European Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014299912002907?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014299912002907?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T22:15:22Z","timestamp":1757974522000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014299912002907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6]]},"references-count":43,"journal-issue":{"issue":"1-3","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["S0014299912002907"],"URL":"https:\/\/doi.org\/10.1016\/j.ejphar.2012.03.030","relation":{},"ISSN":["0014-2999"],"issn-type":[{"type":"print","value":"0014-2999"}],"subject":[],"published":{"date-parts":[[2012,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Gadolinium modulates gentamicin uptake via an endocytosis-independent pathway in HK-2 human renal proximal tubular cell line","name":"articletitle","label":"Article Title"},{"value":"European Journal of Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejphar.2012.03.030","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2012 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}