{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:16:47Z","timestamp":1777382207269,"version":"3.51.4"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81271994, 81301550 and 81371971"],"award-info":[{"award-number":["81271994, 81301550 and 81371971"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Jiangsu Province Health Department Foundation","award":["H201250"],"award-info":[{"award-number":["H201250"]}]},{"DOI":"10.13039\/100000001","name":"Nature Science Foundation","doi-asserted-by":"crossref","award":["BK2011433"],"award-info":[{"award-number":["BK2011433"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pharmacology"],"published-print":{"date-parts":[[2017,2]]},"DOI":"10.1016\/j.ejphar.2016.12.032","type":"journal-article","created":{"date-parts":[[2016,12,22]],"date-time":"2016-12-22T00:01:02Z","timestamp":1482364862000},"page":"131-138","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["Methotrexate prevents epidural fibrosis through endoplasmic reticulum stress signalling pathway"],"prefix":"10.1016","volume":"796","author":[{"given":"Hui","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianqi","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingcheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daxin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gengyao","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.ejphar.2016.12.032_bib1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0304-3959(00)00281-5","article-title":"Central administration of methotrexate reduces mechanical allodynia in an animal model of radiculopathy\/sciatica","volume":"87","author":"Hashizume","year":"2000","journal-title":"Pain"},{"issue":"4","key":"10.1016\/j.ejphar.2016.12.032_bib2","doi-asserted-by":"crossref","first-page":"285","DOI":"10.5505\/tjtes.2013.65475","article-title":"Effects of low-dose methotrexate in spinal cord injury in rats","volume":"19","author":"Bakar","year":"2013","journal-title":"Ulus. Travma ve Acids. Cerrah-. Derg.: Turk. J. Trauma Emerg. Surg.: TJTES"},{"key":"10.1016\/j.ejphar.2016.12.032_bib3","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1097\/00007632-199408150-00004","article-title":"Preventing postlaminectomy adhesion. A new experimental model","volume":"19","author":"Abitbol","year":"1994","journal-title":"Spine"},{"key":"10.1016\/j.ejphar.2016.12.032_bib4","doi-asserted-by":"crossref","first-page":"147","DOI":"10.5114\/fn.2013.35958","article-title":"Nanofiber nets in prevention of cicatrization in spinal procedures. Experimental study","volume":"51","author":"Andrychowski","year":"2013","journal-title":"Folia Neuropathol."},{"key":"10.1016\/j.ejphar.2016.12.032_bib5","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.dld.2011.08.013","article-title":"Liver stiffness correlates with methotrexate cumulative dose in patients with rheumatoid arthritis","volume":"44","author":"Arena","year":"2012","journal-title":"Dig. Liver Dis."},{"key":"10.1016\/j.ejphar.2016.12.032_bib6","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.dld.2011.08.013","article-title":"Liver stiffness correlates with methotrexate cumulative dose in patients with rheumatoid arthritis","volume":"44","author":"Arena","year":"2012","journal-title":"Dig. Liver Dis."},{"key":"10.1016\/j.ejphar.2016.12.032_bib7","first-page":"1096","article-title":"Oxidative effect of methotrexate administration in spinal cord of rabbits","volume":"61","author":"Ayromlou","year":"2011","journal-title":"J. Pak. Med. Assoc."},{"key":"10.1016\/j.ejphar.2016.12.032_bib8","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1093\/toxsci\/kfm067","article-title":"The contribution of methotrexate exposure and host factors on transcriptional variance in human liver","volume":"97","author":"Belinsky","year":"2007","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.ejphar.2016.12.032_bib9","doi-asserted-by":"crossref","first-page":"758","DOI":"10.3171\/2009.6.SPINE08580","article-title":"Use of pimecrolimus to prevent epidural fibrosis in a postlaminectomy rat model","volume":"11","author":"Cemil","year":"2009","journal-title":"J. Neurosurg. Spine"},{"key":"10.1016\/j.ejphar.2016.12.032_bib10","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0009-8981(80)90192-8","article-title":"Modified assay for determination of hydroxyproline in a tissue hydrolyzate","volume":"104","author":"Edwards","year":"1980","journal-title":"Clin. Chim. Acta"},{"key":"10.1016\/j.ejphar.2016.12.032_bib11","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1227\/01.neu.0000333307.02802.04","article-title":"Role of antifibrotic cytokine interferon-gamma in the prevention of postlaminectomy peridural fibrosis in rats","volume":"62","author":"Emmez","year":"2008","journal-title":"Neurosurgery"},{"key":"10.1016\/j.ejphar.2016.12.032_bib12","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1038\/sj.cdd.4401369","article-title":"Hsp70-DnaJ chaperone pair prevents nitric oxide- and CHOP-induced apoptosis by inhibiting translocation of Bax to mitochondria","volume":"11","author":"Gotoh","year":"2004","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.ejphar.2016.12.032_bib13","doi-asserted-by":"crossref","first-page":"534","DOI":"10.5435\/00124635-200609000-00003","article-title":"Failed back surgery syndrome: diagnostic evaluation","volume":"14","author":"Guyer","year":"2006","journal-title":"J. Am. Acad. Orthop. Surg."},{"key":"10.1016\/j.ejphar.2016.12.032_bib14","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1007\/s004030050298","article-title":"Methotrexate induces apoptotic cell death in human keratinocytes","volume":"290","author":"Heenen","year":"1998","journal-title":"Arch. Dermatol. Res."},{"key":"10.1016\/j.ejphar.2016.12.032_bib15","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1089\/ars.2007.1793","article-title":"ER stress signaling and the BCL-2 family of proteins: from adaptation to irreversible cellular damage","volume":"9","author":"Hetz","year":"2007","journal-title":"Antioxid. Redox Signal"},{"key":"10.1016\/j.ejphar.2016.12.032_bib16","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.ejogrb.2011.01.016","article-title":"Methotrexate therapy followed by suction curettage followed by Foley tamponade for caesarean scar pregnancy","volume":"156","author":"Jiang","year":"2011","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"10.1016\/j.ejphar.2016.12.032_bib17","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1158\/1535-7163.MCT-10-0777","article-title":"AMPK and Akt determine apoptotic cell death following perturbations of one-carbon metabolism by regulating ER stress in acute lymphoblastic leukemia","volume":"10","author":"Kuznetsov","year":"2011","journal-title":"Mol. Cancer Ther."},{"issue":"1 Suppl Spine","key":"10.1016\/j.ejphar.2016.12.032_bib18","first-page":"52","article-title":"Mitomycin C in preventing spinal epidural fibrosis in a laminectomy model in rats","volume":"100","author":"Lee","year":"2004","journal-title":"J. Neurosurg."},{"key":"10.1016\/j.ejphar.2016.12.032_bib19","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1097\/BOR.0b013e3283588dbb","article-title":"The role of endoplasmic reticulum stress and the unfolded protein response in fibrosis","volume":"24","author":"Lenna","year":"2012","journal-title":"Curr. Opin. Rheuma."},{"key":"10.1016\/j.ejphar.2016.12.032_bib20","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1152\/physrev.00015.2006","article-title":"Endoplasmic reticulum stress signaling in disease","volume":"86","author":"Marciniak","year":"2006","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.ejphar.2016.12.032_bib21","doi-asserted-by":"crossref","first-page":"319","DOI":"10.2131\/jts.39.319","article-title":"Involvement of epithelial-mesenchymal transition in methotrexate-induced pulmonary fibrosis","volume":"39","author":"Ohbayashi","year":"2014","journal-title":"J. Toxicol. Sci."},{"key":"10.1016\/j.ejphar.2016.12.032_bib22","doi-asserted-by":"crossref","first-page":"33533","DOI":"10.1074\/jbc.273.50.33533","article-title":"Importance of poly(ADP-ribose) polymerase and its cleavage in apoptosis. Lesson from an uncleavable mutant","volume":"273","author":"Oliver","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejphar.2016.12.032_bib23","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1038\/sj.cdd.4401373","article-title":"Roles of CHOP\/GADD153 in endoplasmic reticulum stress","volume":"11","author":"Oyadomari","year":"2004","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.ejphar.2016.12.032_bib24","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.taap.2012.08.034","article-title":"Prodigiosin activates endoplasmic reticulum stress cell death pathway in human breast carcinoma cell lines","volume":"265","author":"Pan","year":"2012","journal-title":"Toxicol. Appl Pharm."},{"key":"10.1016\/j.ejphar.2016.12.032_bib25","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3171\/jns.1993.79.1.0089","article-title":"The reduction of postlaminectomy peridural fibrosis in rabbits by a carbohydrate polymer","volume":"79","author":"Robertson","year":"1993","journal-title":"J. Neurosurg."},{"key":"10.1016\/j.ejphar.2016.12.032_bib26","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1227\/00006123-199604000-00053","article-title":"Association between peridural scar and recurrent radicular pain after lumbar discectomy: magnetic resonance evaluation. ADCON-L European study group","volume":"38","author":"Ross","year":"1996","journal-title":"Neurosurgery"},{"key":"10.1016\/j.ejphar.2016.12.032_bib27","doi-asserted-by":"crossref","first-page":"307","DOI":"10.3109\/17453677008991516","article-title":"Decreased granulation tissue reaction after installment of hyaluronic acid","volume":"41","author":"Rydell","year":"1970","journal-title":"Acta Orthop. Scand."},{"key":"10.1016\/j.ejphar.2016.12.032_bib28","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/s00586-007-0580-y","article-title":"Preventing peridural fibrosis with nonsteroidal anti-inflammatory drugs","volume":"17","author":"Sandoval","year":"2008","journal-title":"Eur. Spine J."},{"key":"10.1016\/j.ejphar.2016.12.032_bib29","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1007\/s00701-012-1302-8","article-title":"Methothrexate attenuates early neutrophil infiltration and the associated lipid peroxidation in the injured spinal cord but does not induce neurotoxicity in the uninjured spinal cord in rats","volume":"154","author":"Sanli","year":"2012","journal-title":"Acta Neurochir."},{"key":"10.1016\/j.ejphar.2016.12.032_bib30","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/0090-3019(83)90418-4","article-title":"Fibrosis of the dura mater. A cause of \"failed back\" syndrome","volume":"19","author":"Siqueira","year":"1983","journal-title":"Surg. Neurol."},{"key":"10.1016\/j.ejphar.2016.12.032_bib31","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ejphar.2008.07.028","article-title":"The effect of 10-hydroxycamptothecine in preventing fibroblast proliferation and epidural scar adhesion after laminectomy in rats","volume":"593","author":"Sun","year":"2008","journal-title":"Eur. J. Pharm."},{"key":"10.1016\/j.ejphar.2016.12.032_bib32","doi-asserted-by":"crossref","first-page":"423","DOI":"10.3171\/SPI-07\/10\/423","article-title":"A comparison of the effectiveness of mitomycin C and 5-fluorouracil in the prevention of peridural adhesion after laminectomy","volume":"7","author":"Sun","year":"2007","journal-title":"J. Neurosurg. Spine"},{"key":"10.1016\/j.ejphar.2016.12.032_bib33","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1038\/sj.cdd.4401365","article-title":"Ischemia-induced neuronal cell death is mediated by the endoplasmic reticulum stress pathway involving CHOP","volume":"11","author":"Tajiri","year":"2004","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.ejphar.2016.12.032_bib34","first-page":"65","article-title":"Mitomycin C induces a delayed and prolonged demyelination and conduction block due to Schwann cell destruction","volume":"27","author":"Westland","year":"1990","journal-title":"Clin. Exp. Neurol."},{"key":"10.1016\/j.ejphar.2016.12.032_bib35","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/0003-9861(61)90291-0","article-title":"The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid","volume":"93","author":"Woessner","year":"1961","journal-title":"Arch. Biochem Biophys."},{"key":"10.1016\/j.ejphar.2016.12.032_bib36","doi-asserted-by":"crossref","first-page":"6965","DOI":"10.1016\/j.biomaterials.2012.06.010","article-title":"Reconstruction of epidural fat with engineered adipose tissue from adipose derived stem cells and PLGA in the rabbit dorsal laminectomy model","volume":"33","author":"Xu","year":"2012","journal-title":"Biomaterials"},{"key":"10.1016\/j.ejphar.2016.12.032_bib37","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.ejphar.2012.11.037","article-title":"Immunomodulatory effectiveness of tacrolimus in preventing epidural scar adhesion after laminectomy in rat model","volume":"699","author":"Yan","year":"2013","journal-title":"Eur. J. Pharm."},{"key":"10.1016\/j.ejphar.2016.12.032_bib38","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.spinee.2011.01.028","article-title":"Application of liposome-encapsulated hydroxycamptothecin in the prevention of epidural scar formation in New Zealand white rabbits","volume":"11","author":"Yang","year":"2011","journal-title":"Spine J."},{"key":"10.1016\/j.ejphar.2016.12.032_bib39","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1007\/s00586-007-0344-8","article-title":"Mitomycin C, 5-fluorouracil, and cyclosporin A prevent epidural fibrosis in an experimental laminectomy model","volume":"16","author":"Yildiz","year":"2007","journal-title":"Eur. Spine J."}],"container-title":["European Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014299916308159?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014299916308159?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T16:49:00Z","timestamp":1568652540000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014299916308159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":39,"alternative-id":["S0014299916308159"],"URL":"https:\/\/doi.org\/10.1016\/j.ejphar.2016.12.032","relation":{},"ISSN":["0014-2999"],"issn-type":[{"value":"0014-2999","type":"print"}],"subject":[],"published":{"date-parts":[[2017,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Methotrexate prevents epidural fibrosis through endoplasmic reticulum stress signalling pathway","name":"articletitle","label":"Article Title"},{"value":"European Journal of Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejphar.2016.12.032","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}