{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T19:27:22Z","timestamp":1793647642308,"version":"4.5.1"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["CUP I83C22001830006"],"award-info":[{"award-number":["CUP I83C22001830006"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["PNRR - M4C2"],"award-info":[{"award-number":["PNRR - M4C2"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pharmacology"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.ejphar.2023.175700","type":"journal-article","created":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T20:49:33Z","timestamp":1680122973000},"page":"175700","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Benzodiazepine diazepam regulates cell surface \u03b21-adrenergic receptor density in human monocytes"],"prefix":"10.1016","volume":"948","author":[{"given":"Paola","family":"Matarrese","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Maccari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucrezia","family":"Gambardella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Vona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Barbagallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Vezzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonino","family":"Stati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Gr\u00f2","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonello","family":"Giovannetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liviana","family":"Catalano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Molinari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Marano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Ambrosio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"8","key":"10.1016\/j.ejphar.2023.175700_bib1","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1210\/me.2002-0054","article-title":"Receptor-mediated adenylyl cyclase activation through XL\u03b1(s), the extra-large variant of the stimulatory G protein \u03b1-subunit","volume":"16","author":"Bastepe","year":"2002","journal-title":"Mol. Endocrinol."},{"issue":"23","key":"10.1016\/j.ejphar.2023.175700_bib2","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2101119118","article-title":"Visualization of \u03b2-adrenergic receptor dynamics and differential localization in cardiomyocytes","volume":"118","author":"Bathe-Peters","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ejphar.2023.175700_bib3","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1471-2121-9-56","article-title":"Delayed internalization and lack of recycling in a beta2-adrenergic receptor fused to the G protein alpha-subunit","volume":"9","author":"Di Certo","year":"2008","journal-title":"BMC Cell Biol."},{"issue":"3","key":"10.1016\/j.ejphar.2023.175700_bib4","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/ncb3307","article-title":"\u03b2-Arrestin drives MAP kinase signalling from clathrin-coated structures after GPCR dissociation","volume":"18","author":"Eichel","year":"2016","journal-title":"Nat. Cell Biol."},{"issue":"10","key":"10.1016\/j.ejphar.2023.175700_bib5","doi-asserted-by":"crossref","first-page":"3355","DOI":"10.4049\/jimmunol.139.10.3355","article-title":"Beta-adrenergic-receptor-mediated suppression of interleukin 2 receptors in human lymphocytes","volume":"139","author":"Feldman","year":"1987","journal-title":"J. Immunol."},{"issue":"5","key":"10.1016\/j.ejphar.2023.175700_bib6","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1161\/HYPERTENSIONAHA.120.15130","article-title":"Role of endothelial G protein-coupled receptor kinase 2 in angioedema","volume":"76","author":"Gambardella","year":"2020","journal-title":"Hypertension"},{"key":"10.1016\/j.ejphar.2023.175700_bib7","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1146\/annurev.neuro.27.070203.144206","article-title":"Desensitization of G protein-coupled receptors and neuronal functions","volume":"27","author":"Gainetdinov","year":"2004","journal-title":"Annu. Rev. Neurosci."},{"issue":"12","key":"10.1016\/j.ejphar.2023.175700_bib8","doi-asserted-by":"crossref","first-page":"2279","DOI":"10.4161\/15548627.2014.991665","article-title":"TSPO interacts with VDAC1 and triggers a ROS-mediated inhibition of mitochondrial quality control","volume":"10","author":"Gatliff","year":"2014","journal-title":"Autophagy"},{"issue":"2","key":"10.1016\/j.ejphar.2023.175700_bib9","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.yexcr.2015.10.032","article-title":"\u03b22-Agonist clenbuterol hinders human monocyte differentiation into dendritic cells","volume":"339","author":"Giordani","year":"2015","journal-title":"Exp. Cell Res."},{"issue":"42","key":"10.1016\/j.ejphar.2023.175700_bib10","doi-asserted-by":"crossref","first-page":"26215","DOI":"10.1016\/S0021-9258(18)47181-5","article-title":"A proline-rich region of the third intracellular loop imparts phenotypic beta 1-versus beta 2-adrenergic receptor coupling and sequestration","volume":"269","author":"Green","year":"1994","journal-title":"J. Biol. Chem."},{"issue":"52","key":"10.1016\/j.ejphar.2023.175700_bib11","doi-asserted-by":"crossref","first-page":"15126","DOI":"10.1073\/pnas.1611023114","article-title":"\u03b22-Adrenergic receptor-dependent chemokine receptor 2 expression regulates leukocyte recruitment to the heart following acute injury","volume":"113","author":"Grisanti","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"6","key":"10.1016\/j.ejphar.2023.175700_bib12","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.molimm.2009.12.013","article-title":"Pro-inflammatory responses in human monocytes are beta1-adrenergic receptor subtype dependent","volume":"47","author":"Grisanti","year":"2010","journal-title":"Mol. Immunol."},{"issue":"4","key":"10.1016\/j.ejphar.2023.175700_bib13","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s00210-009-0392-1","article-title":"Lack of specificity of antibodies directed against human beta-adrenergic receptors","volume":"379","author":"Hamdani","year":"2009","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"issue":"11","key":"10.1016\/j.ejphar.2023.175700_bib14","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1161\/01.RES.0000191541.06788.bb","article-title":"Ischemic neoangiogenesis enhanced by beta2-adrenergic receptor overexpression: a novel role for the endothelial adrenergic system","volume":"97","author":"Iaccarino","year":"2005","journal-title":"Circ. Res."},{"issue":"3","key":"10.1016\/j.ejphar.2023.175700_bib15","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1752-8062.2008.00050.x","article-title":"Enhanced GRK2 expression and desensitization of beta-AR vasodilatation in hypertensive patients","volume":"1","author":"Izzo","year":"2008","journal-title":"Clin Transl Sci"},{"key":"10.1016\/j.ejphar.2023.175700_bib16","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.3389\/fphys.2018.01518","article-title":"The mitochondrial translocator protein and the emerging link between oxidative stress and arrhythmias in the diabetic heart","volume":"9","author":"Ilkan","year":"2018","journal-title":"Front. Physiol."},{"key":"10.1016\/j.ejphar.2023.175700_bib17","doi-asserted-by":"crossref","first-page":"535","DOI":"10.3389\/fbioe.2020.00535","article-title":"DMD Pluripotent stem cell derived cardiac cells recapitulate in vitro human cardiac pathophysiology","volume":"8","author":"Jelinkova","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"1","key":"10.1016\/j.ejphar.2023.175700_bib18","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1006\/jsre.2002.6524","article-title":"G-protein coupled receptor kinase 2 is altered during septic shock in rats","volume":"108","author":"Kadoi","year":"2002","journal-title":"J. Surg. Res."},{"issue":"41","key":"10.1016\/j.ejphar.2023.175700_bib19","doi-asserted-by":"crossref","first-page":"39773","DOI":"10.1074\/jbc.M304482200","article-title":"Resistance of the human beta 1-adrenergic receptor to agonist-mediated down-regulation. Role of the C terminus in determining beta-subtype degradation","volume":"278","author":"Liang","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejphar.2023.175700_bib20","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3389\/fncel.2019.00163","article-title":"Diazepam accelerates GABAAR synaptic exchange and alters intracellular trafficking","volume":"13","author":"Lorenz-Guertin","year":"2019","journal-title":"Front. Cell. Neurosci."},{"key":"10.1016\/j.ejphar.2023.175700_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.biocel.2019.105620","article-title":"Diazepam enhances melanogenesis, melanocyte dendricity and melanosome transport via the PBR\/cAMP\/PKA pathway","volume":"116","author":"Lv","year":"2019","journal-title":"Int. J. Biochem. Cell Biol."},{"issue":"2","key":"10.1016\/j.ejphar.2023.175700_bib22","doi-asserted-by":"crossref","first-page":"512","DOI":"10.3390\/ijms21020512","article-title":"\u03b2-blockers reverse agonist-induced \u03b22-AR downregulation regardless of their signaling profile","volume":"21","author":"Maccari","year":"2020","journal-title":"Int. J. Mol. Sci."},{"issue":"18","key":"10.1016\/j.ejphar.2023.175700_bib23","doi-asserted-by":"crossref","first-page":"15778","DOI":"10.1074\/jbc.M300731200","article-title":"Promiscuous coupling at receptor-G\u03b1 fusion proteins. The receptor of one covalent complex interacts with the \u03b1-subunit of another","volume":"278","author":"Molinari","year":"2003","journal-title":"J. Biol. Chem."},{"issue":"1","key":"10.1016\/j.ejphar.2023.175700_bib24","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1042\/BJ20070803","article-title":"Functional complementation of high-efficiency resonance energy transfer: a new tool for the study of protein binding interactions in living cells","volume":"409","author":"Molinari","year":"2008","journal-title":"Biochem. J."},{"key":"10.1016\/j.ejphar.2023.175700_bib25","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.phrs.2017.01.016","article-title":"\u03b22-adrenergic signaling affects the phenotype of human cardiac progenitor cells through EMT modulation","volume":"127","author":"Pagano","year":"2018","journal-title":"Pharmacol. Res."},{"issue":"4","key":"10.1016\/j.ejphar.2023.175700_bib26","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.jacbts.2018.06.002","article-title":"Carvedilol prevents redox inactivation of cardiomyocyte \u03b21-adrenergic receptors","volume":"3","author":"Park","year":"2018","journal-title":"JACC Basic Transl. Sci."},{"issue":"1","key":"10.1016\/j.ejphar.2023.175700_bib27","doi-asserted-by":"crossref","first-page":"7890","DOI":"10.1038\/s41598-017-06607-z","article-title":"\u03b21-adrenergic receptor O-glycosylation regulates N-terminal cleavage and signaling responses in cardiomyocytes","volume":"7","author":"Park","year":"2017","journal-title":"Sci. Rep."},{"issue":"11","key":"10.1016\/j.ejphar.2023.175700_bib28","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1016\/S0898-6568(03)00099-8","article-title":"Mechanisms of regulation of the expression and function of G protein-coupled receptor kinases","volume":"15","author":"Penela","year":"2003","journal-title":"Cell. Signal."},{"issue":"24","key":"10.1016\/j.ejphar.2023.175700_bib29","doi-asserted-by":"crossref","first-page":"16701","DOI":"10.1074\/jbc.274.24.16701","article-title":"Cardiovascular and metabolic alterations in mice lacking both beta1- and beta2-adrenergic receptors","volume":"274","author":"Rohrer","year":"1999","journal-title":"J. Biol. Chem."},{"issue":"8","key":"10.1016\/j.ejphar.2023.175700_bib30","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/j.amjcard.2010.12.006","article-title":"G protein-coupled receptor kinase 2 in patients with acute myocardial infarction","volume":"107","author":"Santulli","year":"2011","journal-title":"Am. J. Cardiol."},{"issue":"1","key":"10.1016\/j.ejphar.2023.175700_bib31","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s40292-013-0001-8","article-title":"G-protein-coupled receptor kinase 2 and hypertension: molecular insights and pathophysiological mechanisms","volume":"20","author":"Santulli","year":"2013","journal-title":"High Blood Pres. Cardiovasc. Prev."},{"key":"10.1016\/j.ejphar.2023.175700_bib32","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1002\/cyto.a.23358","article-title":"Relative quantification of \u03b2-adrenergic receptor in peripheral blood cells using flow cytometry","volume":"93","author":"Saygin","year":"2018","journal-title":"Cytometry"},{"issue":"37","key":"10.1016\/j.ejphar.2023.175700_bib33","doi-asserted-by":"crossref","first-page":"29082","DOI":"10.1074\/jbc.M909757199","article-title":"Interaction with \u03b2-arrestin determines the difference in internalization behavior between beta1- and beta2-adrenergic receptors","volume":"275","author":"Shiina","year":"2000","journal-title":"J. Biol. Chem."},{"issue":"8","key":"10.1016\/j.ejphar.2023.175700_bib34","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/s12265-015-9646-0","article-title":"Integrating GRK2 and NFkappaB in the pathophysiology of cardiac hypertrophy","volume":"8","author":"Sorriento","year":"2015","journal-title":"Cardiovasc Transl Res"},{"issue":"3","key":"10.1016\/j.ejphar.2023.175700_bib35","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/S0026-895X(25)08956-4","article-title":"Distinct regulation of beta1- and beta2-adrenergic receptors in Chinese hamster fibroblasts","volume":"41","author":"Suzuki","year":"1992","journal-title":"Mol. Pharmacol."},{"issue":"1","key":"10.1016\/j.ejphar.2023.175700_bib36","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-34451-2","article-title":"Cardiac-specific conditional knockout of the 18-kDa mitochondrial translocator protein protects from pressure overload induced heart failure","volume":"8","author":"Thai","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ejphar.2023.175700_bib40","doi-asserted-by":"crossref","first-page":"32835","DOI":"10.1038\/srep32835","article-title":"Activation of \u03b2-adrenergic receptors is required for elevated \u03b11A-adrenoreceptors expression and signaling in mesenchymal stromal cells","volume":"6","author":"Tyurin-Kuzmin","year":"2016","journal-title":"Sci. Rep."},{"issue":"6","key":"10.1016\/j.ejphar.2023.175700_bib37","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.tips.2006.04.009","article-title":"Subtype-specific alpha1- and beta-adrenoceptor signaling in the heart","volume":"27","author":"Xiao","year":"2006","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.ejphar.2023.175700_bib38","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.yjmcc.2021.01.012","article-title":"\u03b21-adrenergic receptor N-terminal cleavage by ADAM17; the mechanism for redox-dependent downregulation of cardiomyocyte \u03b21-adrenergic receptors","volume":"154","author":"Zhu","year":"2021","journal-title":"J. Mol. Cell. Cardiol."},{"issue":"3","key":"10.1016\/j.ejphar.2023.175700_bib39","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1152\/physrev.00026.2013","article-title":"Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release","volume":"94","author":"Zorov","year":"2014","journal-title":"Physiol. Rev."}],"container-title":["European Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001429992300211X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001429992300211X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T23:13:59Z","timestamp":1757978039000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S001429992300211X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":40,"alternative-id":["S001429992300211X"],"URL":"https:\/\/doi.org\/10.1016\/j.ejphar.2023.175700","relation":{},"ISSN":["0014-2999"],"issn-type":[{"value":"0014-2999","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Benzodiazepine diazepam regulates cell surface \u03b21-adrenergic receptor density in human monocytes","name":"articletitle","label":"Article Title"},{"value":"European Journal of Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejphar.2023.175700","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"175700"}}