{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T05:09:41Z","timestamp":1778648981935,"version":"3.51.4"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82100818"],"award-info":[{"award-number":["82100818"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["11903800"],"award-info":[{"award-number":["11903800"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pharmacology"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.ejphar.2025.178425","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:15:40Z","timestamp":1764634540000},"page":"178425","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Activation of 5-HT2A receptor by TCB2 improves bladder function in rats with bilateral pelvic nerve injury via TRPV4"],"prefix":"10.1016","volume":"1010","author":[{"given":"Lin","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengyi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifang","family":"Kuang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dachao","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5835-1668","authenticated-orcid":false,"given":"Lixuan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baojun","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haijun","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianshu","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejphar.2025.178425_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.mvr.2023.104516","article-title":"Activation of renal vascular smooth muscle TRPV4 channels by 5-hydroxytryptamine impairs kidney function in neonatal pigs","volume":"148","author":"Afolabi","year":"2023","journal-title":"Microvasc. Res."},{"key":"10.1016\/j.ejphar.2025.178425_bib2","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1002\/nau.21212","article-title":"Effects of TRPV4 cation channel activation on the primary bladder afferent activities of the rat","volume":"31","author":"Aizawa","year":"2012","journal-title":"Neurourol. Urodyn."},{"key":"10.1016\/j.ejphar.2025.178425_bib3","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/JID.2015.388","article-title":"Involvement of TRPV4 in serotonin-evoked scratching","volume":"136","author":"Akiyama","year":"2016","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.ejphar.2025.178425_bib4","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.brainres.2019.03.009","article-title":"Streptozotocin-induced diabetes causes upregulation of serotonin (5-HT)(2A\/C) receptors in lumbosacral cord motoneurons and down regulation of serotonergic paraneurons in the urethra","volume":"1715","author":"Cao","year":"2019","journal-title":"Brain Res."},{"key":"10.1016\/j.ejphar.2025.178425_bib5","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/bju.13964","article-title":"Chronic spinal cord injury causes upregulation of serotonin (5-HT)(2A) and 5-HT(2C) receptors in lumbosacral cord motoneurons","volume":"121","author":"Cao","year":"2018","journal-title":"BJU Int."},{"key":"10.1016\/j.ejphar.2025.178425_bib6","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1136\/gut.2009.192567","article-title":"Potentiation of TRPV4 signalling by histamine and serotonin: an important mechanism for visceral hypersensitivity","volume":"59","author":"Cenac","year":"2010","journal-title":"Gut"},{"key":"10.1016\/j.ejphar.2025.178425_bib7","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1016\/j.juro.2012.11.049","article-title":"The effect of the 5-HT2A\/2C receptor agonist DOI on micturition in rats with chronic spinal cord injury","volume":"189","author":"Chen","year":"2013","journal-title":"J. Urol."},{"key":"10.1016\/j.ejphar.2025.178425_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejphar.2024.176909","article-title":"The serotonin(5-HT)2A receptor is involved in the hypersensitivity of bladder afferent neurons in cyclophosphamide-induced cystitis","volume":"982","author":"Chen","year":"2024","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2025.178425_bib9","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1111\/iju.14267","article-title":"Serotonin in the rat prefrontal cortex controls the micturition reflex through 5-hydroxytryptamine 2A and 5-hydroxytryptamine 7 receptors","volume":"27","author":"Chiba","year":"2020","journal-title":"Int. J. Urol. : Official Journal of the Japanese Urological Association"},{"key":"10.1016\/j.ejphar.2025.178425_bib10","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.brainresbull.2020.05.010","article-title":"Expression and functional characterization of transient receptor potential vanilloid 4 in the dorsal root ganglion and spinal cord of diabetic rats with mechanical allodynia","volume":"162","author":"Cui","year":"2020","journal-title":"Brain Res. Bull."},{"key":"10.1016\/j.ejphar.2025.178425_bib11","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/S0090-4295(01)01636-3","article-title":"Influence of central serotonergic mechanisms on lower urinary tract function","volume":"59","author":"de Groat","year":"2002","journal-title":"Urology"},{"key":"10.1016\/j.ejphar.2025.178425_bib12","doi-asserted-by":"crossref","first-page":"288","DOI":"10.5213\/inj.2244162.081","article-title":"Effects of intravesical electrical stimulation on urinary adenosine triphosphate and nitric oxide in rats with detrusor underactivity induced by bilateral pelvic nerve crush injury: the possible underlying mechanism","volume":"26","author":"Deng","year":"2022","journal-title":"Int. Neurourol. J."},{"key":"10.1016\/j.ejphar.2025.178425_bib13","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.eururo.2018.05.020","article-title":"Intravesical activation of the cation channel TRPV4 improves bladder function in a rat model for detrusor underactivity","volume":"74","author":"Deruyver","year":"2018","journal-title":"Eur. Urol."},{"key":"10.1016\/j.ejphar.2025.178425_bib14","doi-asserted-by":"crossref","first-page":"E86","DOI":"10.1111\/bju.14649","article-title":"Functional and molecular characterisation of the bilateral pelvic nerve crush injury rat model for neurogenic detrusor underactivity","volume":"123","author":"Dewulf","year":"2019","journal-title":"BJU Int."},{"key":"10.1016\/j.ejphar.2025.178425_bib15","doi-asserted-by":"crossref","first-page":"19084","DOI":"10.1073\/pnas.1005333107","article-title":"Inhibition of the cation channel TRPV4 improves bladder function in mice and rats with cyclophosphamide-induced cystitis","volume":"107","author":"Everaerts","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1016\/j.ejphar.2025.178425_bib16","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s11255-020-02668-8","article-title":"Establishment of animal model manifested as bladder neurogenic changes generated by bilateral pelvic nerve injury in male rats","volume":"53","author":"Ge","year":"2021","journal-title":"Int. Urol. Nephrol."},{"key":"10.1016\/j.ejphar.2025.178425_bib17","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1007\/s12031-013-0033-5","article-title":"Increased TRPV4 expression in urinary bladder and lumbosacral dorsal root ganglia in mice with chronic overexpression of NGF in urothelium","volume":"51","author":"Girard","year":"2013","journal-title":"J. Mol. Neurosci. : MN"},{"key":"10.1016\/j.ejphar.2025.178425_bib18","doi-asserted-by":"crossref","DOI":"10.3390\/ijms19041256","article-title":"Ca2+ regulation of TRP ion channels","volume":"19","author":"Hasan","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.ejphar.2025.178425_bib19","doi-asserted-by":"crossref","DOI":"10.1172\/JCI161507","article-title":"A TRPV4-dependent neuroimmune axis in the spinal cord promotes neuropathic pain","volume":"133","author":"Hu","year":"2023","journal-title":"J. Clin. Investig."},{"key":"10.1016\/j.ejphar.2025.178425_bib20","doi-asserted-by":"crossref","first-page":"287","DOI":"10.4103\/tcmj.tcmj_284_20","article-title":"Pathogenesis evidence from human and animal models of detrusor underactivity","volume":"34","author":"Jhang","year":"2022","journal-title":"Tzu Chi Med. J."},{"key":"10.1016\/j.ejphar.2025.178425_bib21","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1002\/nau.22487","article-title":"Roles of adenosine A1 and A2A receptors in the control of micturition in rats","volume":"33","author":"Kitta","year":"2014","journal-title":"Neurourol. Urodyn."},{"key":"10.1016\/j.ejphar.2025.178425_bib22","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1016\/j.peptides.2009.07.025","article-title":"Involvement of orexin-A on micturition reflex in normal and cyclophosphamide-induced cystitis bladder in rat","volume":"30","author":"Kobayashi","year":"2009","journal-title":"Peptides"},{"key":"10.1016\/j.ejphar.2025.178425_bib23","doi-asserted-by":"crossref","DOI":"10.1177\/0963689720909387","article-title":"Amniotic fluid stem cells improve rat bladder dysfunction after pelvic nerve transection","volume":"29","author":"Liang","year":"2020","journal-title":"Cell Transplant."},{"key":"10.1016\/j.ejphar.2025.178425_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaci.2017.05.051","article-title":"Transient receptor potential vanilloid 4-expressing macrophages and keratinocytes contribute differentially to allergic and nonallergic chronic itch","volume":"141","author":"Luo","year":"2018","journal-title":"J. Allergy Clin. Immunol."},{"issue":"Suppl. 1","key":"10.1016\/j.ejphar.2025.178425_bib25","article-title":"Regulatory effects of 5-Hydroxytryptamine receptors on voiding function","volume":"32","author":"Matsumoto-Miyai","year":"2015","journal-title":"Adv. Ther."},{"key":"10.1016\/j.ejphar.2025.178425_bib26","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1146\/annurev-pharmtox-010617-052832","article-title":"TRP channels as potential drug targets","volume":"58","author":"Moran","year":"2018","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/j.ejphar.2025.178425_bib27","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.eururo.2018.07.037","article-title":"Detrusor underactivity and the underactive bladder: a systematic review of preclinical and clinical studies","volume":"74","author":"Osman","year":"2018","journal-title":"Eur. Urol."},{"issue":"Suppl. 2","key":"10.1016\/j.ejphar.2025.178425_bib28","first-page":"S120","article-title":"The role of central 5-hydroxytryptamine (5-HT, serotonin) receptors in the control of micturition","volume":"147","author":"Ramage","year":"2006","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2025.178425_bib29","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1038\/s41374-021-00593-7","article-title":"Serotonin-induced vascular permeability is mediated by transient receptor potential vanilloid 4 in the airways and upper gastrointestinal tract of mice","volume":"101","author":"Retamal","year":"2021","journal-title":"Laboratory Investigation; a Journal of Technical Methods and Pathology"},{"key":"10.1016\/j.ejphar.2025.178425_bib30","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1111\/bph.15733","article-title":"Glucosylsphingosine evokes pruritus via activation of 5-HT2A receptor and TRPV4 in sensory neurons","volume":"179","author":"Sanjel","year":"2022","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2025.178425_bib31","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.juro.2016.02.064","article-title":"Promising effects of a novel EP2 and EP3 receptor dual agonist, ONO-8055, on neurogenic underactive bladder in a rat lumbar canal stenosis model","volume":"196","author":"Sekido","year":"2016","journal-title":"J. Urol."},{"key":"10.1016\/j.ejphar.2025.178425_bib32","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1111\/bph.13941","article-title":"Brain serotoninergic nervous system is involved in bombesin-induced frequent urination through brain 5-HT7 receptors in rats","volume":"174","author":"Shimizu","year":"2017","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.ejphar.2025.178425_bib33","doi-asserted-by":"crossref","DOI":"10.3389\/fruro.2022.1086179","article-title":"Repeated variate stress increased voiding frequency and altered TrpV1 and TrpV4 transcript expression in lower urinary tract (LUT) pathways in female mice","volume":"2","author":"Sidwell","year":"2023","journal-title":"Frontiers in urology"},{"key":"10.1016\/j.ejphar.2025.178425_bib34","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1002\/nau.22653","article-title":"Does defective volume sensation contribute to detrusor underactivity?","volume":"34","author":"Smith","year":"2015","journal-title":"Neurourol. Urodyn."},{"key":"10.1016\/j.ejphar.2025.178425_bib35","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1002\/nau.23790","article-title":"Effect of TRPV4 activation in a rat model of detrusor underactivity induced by bilateral pelvic nerve crush injury","volume":"37","author":"Takaoka","year":"2018","journal-title":"Neurourol. Urodyn."},{"key":"10.1016\/j.ejphar.2025.178425_bib36","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/bju.12684","article-title":"Serotonin (5-HT)2A\/2C receptor agonist (2,5-dimethoxy-4-idophenyl)-2-aminopropane hydrochloride (DOI) improves voiding efficiency in the diabetic rat","volume":"116","author":"Tu","year":"2015","journal-title":"BJU Int."},{"issue":"Suppl. 1","key":"10.1016\/j.ejphar.2025.178425_bib37","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1007\/s11255-014-0808-9","article-title":"Pathophysiology and animal modeling of underactive bladder","volume":"46","author":"Tyagi","year":"2014","journal-title":"Int. Urol. Nephrol."},{"key":"10.1016\/j.ejphar.2025.178425_bib38","article-title":"Underactive bladder and detrusor underactivity: new advances and prospectives","volume":"24","author":"Wang","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.ejphar.2025.178425_bib39","doi-asserted-by":"crossref","DOI":"10.3390\/biom13081260","article-title":"Transcriptomics reveals molecular features of the bilateral pelvic nerve injury rat model of detrusor underactivity","volume":"13","author":"Wang","year":"2023","journal-title":"Biomolecules"},{"key":"10.1016\/j.ejphar.2025.178425_bib40","doi-asserted-by":"crossref","first-page":"F1217","DOI":"10.1152\/ajprenal.00198.2018","article-title":"Reduced bladder responses to capsaicin and GSK-1016790A in retired-breeder female rats with diminished volume sensitivity","volume":"315","author":"Wen","year":"2018","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"10.1016\/j.ejphar.2025.178425_bib41","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1002\/nau.23517","article-title":"Characterization of voiding function and structural bladder changes in a rat model of neurogenic underactive bladder disease","volume":"37","author":"Weyne","year":"2018","journal-title":"Neurourol. Urodyn."},{"key":"10.1016\/j.ejphar.2025.178425_bib42","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/luts.12070","article-title":"Detrusor underactivity: the current concept of the pathophysiology","volume":"6","author":"Yoshida","year":"2014","journal-title":"Low. Urin. Tract. Symptoms"},{"key":"10.1016\/j.ejphar.2025.178425_bib43","doi-asserted-by":"crossref","DOI":"10.1177\/17448069221087583","article-title":"Spinal 5-HT2A receptor is involved in electroacupuncture inhibition of chronic pain","volume":"18","author":"Yuan","year":"2022","journal-title":"Mol. Pain"},{"key":"10.1016\/j.ejphar.2025.178425_bib44","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1007\/s11255-023-03659-1","article-title":"Activation of the adenosine A1 receptor in the Lumbosacral spinal cord improves bladder overactivity in rats with cystitis induced by cyclophosphamide","volume":"55","author":"Zhang","year":"2023","journal-title":"Int. Urol. Nephrol."},{"key":"10.1016\/j.ejphar.2025.178425_bib45","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1038\/s41392-023-01464-x","article-title":"TRP (transient receptor potential) ion channel family: structures, biological functions and therapeutic interventions for diseases","volume":"8","author":"Zhang","year":"2023","journal-title":"Signal Transduct Target Ther"},{"key":"10.1016\/j.ejphar.2025.178425_bib46","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1111\/jcmm.13100","article-title":"Carbenoxolone inhibits TRPV4 channel-initiated oxidative urothelial injury and ameliorates cyclophosphamide-induced bladder dysfunction","volume":"21","author":"Zhang","year":"2017","journal-title":"J. Cell Mol. Med."}],"container-title":["European Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014299925011793?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014299925011793?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T04:16:44Z","timestamp":1778645804000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014299925011793"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":46,"alternative-id":["S0014299925011793"],"URL":"https:\/\/doi.org\/10.1016\/j.ejphar.2025.178425","relation":{},"ISSN":["0014-2999"],"issn-type":[{"value":"0014-2999","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Activation of 5-HT2A receptor by TCB2 improves bladder function in rats with bilateral pelvic nerve injury via TRPV4","name":"articletitle","label":"Article Title"},{"value":"European Journal of Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejphar.2025.178425","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"178425"}}