{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T01:23:08Z","timestamp":1778289788613,"version":"3.51.4"},"reference-count":40,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Pharmaceutical Sciences"],"published-print":{"date-parts":[[2010,8]]},"DOI":"10.1016\/j.ejps.2010.04.013","type":"journal-article","created":{"date-parts":[[2010,4,29]],"date-time":"2010-04-29T04:37:00Z","timestamp":1272515820000},"page":"404-411","source":"Crossref","is-referenced-by-count":21,"title":["HIV-1 Tat-peptide inhibits protein kinase C and protein kinase A through substrate competition"],"prefix":"10.1016","volume":"40","author":[{"given":"Elina","family":"Ekokoski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olli","family":"Aitio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kid","family":"T\u00f6rnquist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jari","family":"Yli-Kauhaluoma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raimo K.","family":"Tuominen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejps.2010.04.013_bib1","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1016\/j.cellsig.2006.01.005","article-title":"Sphingosylphosphorylcholine enhances calcium entry in thyroid FRO cells by a mechanism dependent on protein kinase C","volume":"18","author":"Afrasiabi","year":"2006","journal-title":"Cell. Signalling"},{"key":"10.1016\/j.ejps.2010.04.013_bib2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/S0014-5793(96)01370-1","article-title":"Molecular basis for the substrate specificity of protein kinase B; comparison with MAPKAP kinase -1 and p70 S6 kinase","volume":"399","author":"Alessi","year":"1996","journal-title":"FEBS Lett."},{"key":"10.1016\/j.ejps.2010.04.013_bib3","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/S0006-291X(87)80113-4","article-title":"Substrate-specific stimulation of protein kinase C by polyvalent anions","volume":"147","author":"Bazzi","year":"1987","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ejps.2010.04.013_bib4","doi-asserted-by":"crossref","first-page":"40003","DOI":"10.1074\/jbc.M507274200","article-title":"Substrate specificity and activity regulation of protein kinase MELK","volume":"280","author":"Beullens","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib5","doi-asserted-by":"crossref","first-page":"3969","DOI":"10.1021\/jm900229p","article-title":"Design, synthesis and biological activity of isophthalic acid derivatives targeted to the C1 domain of protein kinase C","volume":"52","author":"Boije af Genn\u00e4s","year":"2009","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib6","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.1016\/j.bbamem.2007.06.003","article-title":"Cell-permeable peptides induce dose- and length-dependent cytotoxic effects","volume":"1768","author":"Cardozo","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ejps.2010.04.013_bib7","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.jconrel.2006.10.031","article-title":"The taming of the cell penetrating domain of the HIV Tat: Myths and realities","volume":"117","author":"Chauhan","year":"2007","journal-title":"J. Control. Rel."},{"key":"10.1016\/j.ejps.2010.04.013_bib8","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1016\/S0021-9258(17)36041-6","article-title":"A potent synthetis peptide inhibitor of the cAMP-dependent protein kinase","volume":"261","author":"Cheng","year":"1986","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib9","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/nrd773","article-title":"Protein kinases\u2014the major drug targets of the twenty-first century?","volume":"1","author":"Cohen","year":"2002","journal-title":"Nat. Rev. Drug Discovery"},{"key":"10.1016\/j.ejps.2010.04.013_bib10","doi-asserted-by":"crossref","first-page":"4426","DOI":"10.1093\/emboj\/17.15.4426","article-title":"Mitogen- and stress-activated protein kinase-1 (MSK1) is directly acivated by MAPK and SAPK2\/p38, and may mediate activation of CREB","volume":"17","author":"Deak","year":"1998","journal-title":"EMBO J."},{"key":"10.1016\/j.ejps.2010.04.013_bib11","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1111\/j.1600-0854.2007.00572.x","article-title":"A comprehensive model for the cellular uptake of cationic cell-penetrating peptides","volume":"8","author":"Duchardt","year":"2007","journal-title":"Traffic"},{"key":"10.1016\/j.ejps.2010.04.013_bib12","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/j.addr.2009.06.001","article-title":"Recent advances in the use of cell-penetrating peptides for medical and biological applications","volume":"61","author":"Fonseca","year":"2009","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.ejps.2010.04.013_bib13","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1242\/jcs.02460","article-title":"Cationic cell-penetrating peptides interfere with TNF signalling by induction of TNF receptor internalization","volume":"118","author":"Fotin-Mleczek","year":"2005","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ejps.2010.04.013_bib14","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S1040-8428(00)00058-5","article-title":"Staurosporine analogues\u2014pharmacological toys or useful antitumour agents?","volume":"34","author":"Gescher","year":"2000","journal-title":"Crit. Rev. Oncol. Hematol."},{"key":"10.1016\/j.ejps.2010.04.013_bib15","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/S0092-8674(00)81066-1","article-title":"Structural basis for the autoinhibition of calcium\/calmodulin-dependent protein kinase I","volume":"84","author":"Goldberg","year":"1996","journal-title":"Cell"},{"key":"10.1016\/j.ejps.2010.04.013_bib16","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca2+ indicators with greatly improved fluorescence properties","volume":"260","author":"Grynkiewicz","year":"1985","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib17","doi-asserted-by":"crossref","first-page":"28837","DOI":"10.1074\/jbc.274.41.28837","article-title":"Multifaceted activities of the HIV-1 transactivator of transcription, Tat","volume":"274","author":"Jeang","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib18","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1038\/sj.bjp.0706279","article-title":"Characterization of cell-penetrating peptide-mediated peptide delivery","volume":"145","author":"Jones","year":"2005","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.ejps.2010.04.013_bib19","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1152\/physiol.00022.2006","article-title":"Peptides targeting protein kinases: strategies and implications","volume":"21","author":"Kaidanovich-Beilin","year":"2006","journal-title":"Physiology"},{"key":"10.1016\/j.ejps.2010.04.013_bib20","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1126\/science.1104607","article-title":"Crystal structure of a complex between the catalytic and regulatory (RI\u03b1) subunits of PKA","volume":"307","author":"Kim","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.ejps.2010.04.013_bib21","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1042\/0264-6021:3440189","article-title":"Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase","volume":"344","author":"Kobayashi","year":"1999","journal-title":"Biochem. J."},{"key":"10.1016\/j.ejps.2010.04.013_bib22","doi-asserted-by":"crossref","first-page":"7493","DOI":"10.1038\/sj.onc.1209087","article-title":"AKT crystal structure and AKT-spesific inhibitors","volume":"24","author":"Kumar","year":"2005","journal-title":"Oncogene"},{"key":"10.1016\/j.ejps.2010.04.013_bib23","first-page":"6413","article-title":"A requirement of hydrophobic and basic amino acid residues for substrate recognition by Ca2+\/calmodulin-dependent protein kinase Ia","volume":"91","author":"Lee","year":"1994","journal-title":"Biochemistry"},{"key":"10.1016\/j.ejps.2010.04.013_bib24","doi-asserted-by":"crossref","first-page":"13906","DOI":"10.1016\/S0021-9258(19)85188-8","article-title":"Activation of protein kinase C by selective binding of arginine-rich polypeptides","volume":"268","author":"Leventhal","year":"1993","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib25","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1126\/science.1075762","article-title":"The protein kinase complement of the human genome","volume":"298","author":"Manning","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.ejps.2010.04.013_bib26","doi-asserted-by":"crossref","first-page":"4438","DOI":"10.1021\/bi961947+","article-title":"Crystal structure of a polyhistidine-tagged recombinant caalytic subunit of cMAP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine","volume":"36","author":"Narayana","year":"1997","journal-title":"Biochemistry"},{"key":"10.1016\/j.ejps.2010.04.013_bib27","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1074\/jbc.272.2.952","article-title":"Determination of the specific substrate sequence motifs of protein kinase C isozymes","volume":"272","author":"Nishikawa","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib28","doi-asserted-by":"crossref","first-page":"8383","DOI":"10.1016\/S0021-9258(17)37205-8","article-title":"Intrapeptide regulation of protein kinase C","volume":"269","author":"Orr","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib29","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/j.1432-1033.1994.tb18979.x","article-title":"P2 protamines are phosphorylated in vitro by protein kinase C, whereas P1 protamines prefer cAMP-dependent protein kinase. A comparative study of five mammalian species","volume":"223","author":"Pirhonen","year":"1994","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.ejps.2010.04.013_bib30","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1095\/biolreprod48.4.821","article-title":"In vitro phosphorylation sites of stallion and bull P1-protamines for cyclic adenosine 3\u2019,5\u2019-monophosphate-dependent protein kinase and protein kinase C","volume":"48","author":"Pirhonen","year":"1993","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ejps.2010.04.013_bib31","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.addr.2007.08.040","article-title":"Protein and peptide transduction, twenty years later a happy birthday","volume":"60","author":"Prochiantz","year":"2008","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.ejps.2010.04.013_bib32","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1128\/MCB.18.2.790","article-title":"Activation of the mitogen-activated protein kinase\/extracellular signal-regulated kinase pathway by conventional, novel, and atypical protein kinase C isotypes","volume":"18","author":"Sch\u00f6nwasser","year":"1998","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.ejps.2010.04.013_bib33","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1152\/physrev.00034.2007","article-title":"Structural basis of protein kinase C isoform function","volume":"88","author":"Steinberg","year":"2008","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.ejps.2010.04.013_bib34","doi-asserted-by":"crossref","first-page":"40576","DOI":"10.1074\/jbc.M405560200","article-title":"Autophosphorylation suppresses whereas kinase inhibition augments the translocation of protein kinase C\u03b1 in response to diacylglycerol","volume":"279","author":"Stensman","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib35","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1074\/jbc.M110017200","article-title":"Possible existence of common internalization mechanisms among arginine-rich peptides","volume":"277","author":"Suzuki","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2010.04.013_bib36","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1002\/ddr.10399","article-title":"Screening of natural compounds and their derivatives as potential protein kinase C inhibitors","volume":"63","author":"Tammela","year":"2004","journal-title":"Drug Dev. Res."},{"key":"10.1016\/j.ejps.2010.04.013_bib37","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.bbapap.2005.08.024","article-title":"Dynamics of signaling by PKA","volume":"1754","author":"Taylor","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ejps.2010.04.013_bib38","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1096\/fj.05-5523com","article-title":"Cargo-dependent mode of uptake and bioavailability of TAT-containing proteins and peptides in living cells","volume":"20","author":"T\u00fcnnemann","year":"2006","journal-title":"FASEB J."},{"key":"10.1016\/j.ejps.2010.04.013_bib39","doi-asserted-by":"crossref","first-page":"18","DOI":"10.7150\/ijms.6.18","article-title":"Transporter molecules influence the gene expression in HeLa cells","volume":"6","author":"Waldeck","year":"2009","journal-title":"Int. J. Med. Sci."},{"key":"10.1016\/j.ejps.2010.04.013_bib40","doi-asserted-by":"crossref","first-page":"36372","DOI":"10.1074\/jbc.M505031200","article-title":"A single pair of acidic residues in the kinase major groove mediates strong substrate preference for P-2 or P-5 arginine in the AGC, CAMK, and STE kinase families","volume":"280","author":"Zhu","year":"2005","journal-title":"J. Biol. Chem."}],"container-title":["European Journal of Pharmaceutical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0928098710001582?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0928098710001582?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T17:31:41Z","timestamp":1635269501000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0928098710001582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8]]},"references-count":40,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["S0928098710001582"],"URL":"https:\/\/doi.org\/10.1016\/j.ejps.2010.04.013","relation":{},"ISSN":["0928-0987"],"issn-type":[{"value":"0928-0987","type":"print"}],"subject":[],"published":{"date-parts":[[2010,8]]}}}