{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T18:58:08Z","timestamp":1788721088084,"version":"build-2803163510"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T00:00:00Z","timestamp":1722038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006209","name":"Radboud University Medical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006209","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pharmaceutical Sciences"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1016\/j.ejps.2024.106868","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T17:19:26Z","timestamp":1722273566000},"page":"106868","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["The potential of enteroids derived from children and adults to study age-dependent differences in intestinal CYP3A4\/5 metabolism"],"prefix":"10.1016","volume":"201","author":[{"given":"Eva J.","family":"Streekstra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marit","family":"Keuper-Navis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen J.W.M.","family":"van den Heuvel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"van den Broek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rick","family":"Greupink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martijn W.J.","family":"Stommel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem P.","family":"de Boode","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanne M.B.I.","family":"Botden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans G.M.","family":"Russel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evita","family":"van de Steeg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia N.","family":"de Wildt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejps.2024.106868_bib0001","doi-asserted-by":"crossref","first-page":"10140","DOI":"10.1038\/s41598-019-46497-x","article-title":"Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function","volume":"9","author":"Altay","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ejps.2024.106868_bib0002","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1038\/s41467-017-01027-z","article-title":"Comprehensive analysis of normal adjacent to tumor transcriptomes","volume":"8","author":"Aran","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ejps.2024.106868_bib0003","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.devcel.2018.02.016","article-title":"From 3D Organoids back to 2D Enteroids","volume":"44","author":"Braverman","year":"2018","journal-title":"Dev. Cell"},{"key":"10.1016\/j.ejps.2024.106868_bib0004","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1111\/bcp.13459","article-title":"Predicting CYP3A-mediated midazolam metabolism in critically ill neonates, infants, children and adults with inflammation and organ failure","volume":"84","author":"Brussee","year":"2018","journal-title":"Br. J. Clin. Pharmacol."},{"key":"10.1016\/j.ejps.2024.106868_bib0005","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1002\/psp4.12295","article-title":"First-pass CYP3A-mediated metabolism of midazolam in the gut wall and liver in preterm neonates","volume":"7","author":"Brussee","year":"2018","journal-title":"CPT Pharmacomet. Syst. Pharmacol."},{"key":"10.1016\/j.ejps.2024.106868_bib0006","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1023\/A:1010914624111","article-title":"Characterizing the expression of CYP3A4 and efflux transporters (P-gp, MRP1, and MRP2) in CYP3A4-transfected Caco-2 cells after induction with sodium butyrate and the Phorbol Ester 12-O-Tetradecanoylphorbol-13-Acetate","volume":"18","author":"Cummins","year":"2001","journal-title":"Pharm. Res."},{"key":"10.1016\/j.ejps.2024.106868_bib0007","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.pharmthera.2018.06.013","article-title":"Post-translational modifications of transporters","volume":"192","author":"Czuba","year":"2018","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.ejps.2024.106868_bib0008","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijpharm.2024.123962","article-title":"Expression of intestinal drug transporter proteins and metabolic enzymes in neonatal and pediatric patients","volume":"654","author":"de Waal","year":"2024","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.ejps.2024.106868_bib0009","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1046\/j.1365-2125.2002.01223.x","article-title":"Pharmacokinetics and metabolism of oral midazolam in preterm infants","volume":"53","author":"de Wildt","year":"2002","journal-title":"Br. J. Clin. Pharmacol."},{"key":"10.1016\/j.ejps.2024.106868_bib0010","doi-asserted-by":"crossref","first-page":"235","DOI":"10.2165\/00003088-200241040-00001","article-title":"The mucosa of the small intestine","volume":"41","author":"Doherty","year":"2002","journal-title":"Clin. Pharmacokinet."},{"key":"10.1016\/j.ejps.2024.106868_bib0011","doi-asserted-by":"crossref","first-page":"3380","DOI":"10.1038\/s41596-020-0379-4","article-title":"Establishment of patient-derived cancer organoids for drug-screening applications","volume":"15","author":"Driehuis","year":"2020","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ejps.2024.106868_bib0012","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1002\/cpt.967","article-title":"Protein abundance of clinically relevant drug-metabolizing enzymes in the human liver and intestine: a comparative analysis in paired tissue specimens","volume":"104","author":"Drozdzik","year":"2018","journal-title":"Clin. Pharmacol. Ther."},{"key":"10.1016\/j.ejps.2024.106868_bib0013","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.ejpb.2023.08.011","article-title":"Quantification of drug metabolising enzymes and transporter proteins in the paediatric duodenum via LC-MS\/MS proteomics using a QconCAT technique","volume":"191","author":"Goelen","year":"2023","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.ejps.2024.106868_bib0014","doi-asserted-by":"crossref","first-page":"13331","DOI":"10.1021\/acs.analchem.8b02845","article-title":"A monolayer of primary colonic epithelium generated on a scaffold with a gradient of stiffness for drug transport studies","volume":"90","author":"Gunasekara","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ejps.2024.106868_bib0015","doi-asserted-by":"crossref","first-page":"681","DOI":"10.3109\/00365521.2014.898326","article-title":"Surface area of the digestive tract - revisited","volume":"49","author":"Helander","year":"2014","journal-title":"Scand. J. Gastroenterol."},{"key":"10.1016\/j.ejps.2024.106868_bib0016","doi-asserted-by":"crossref","DOI":"10.1124\/dmd.123.001270","article-title":"Use of developmental Midazolam and 1-hydroxymidazolam data with pediatric physiologically based modelling to assess CYP3A4 and UGT2B4 ontogeny in vivo","author":"Johnson","year":"2023","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.ejps.2024.106868_bib0017","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1046\/j.1365-2125.2001.01370.x","article-title":"Enterocytic CYP3A4 in a paediatric population: developmental changes and the effect of coeliac disease and cystic fibrosis","volume":"51","author":"Johnson","year":"2001","journal-title":"Br. J. Clin. Pharmacol."},{"key":"10.1016\/j.ejps.2024.106868_bib0018","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1124\/dmd.121.000559","article-title":"Ontogeny of small intestinal drug transporters and metabolizing enzymes based on targeted quantitative proteomics","volume":"49","author":"Kiss","year":"2021","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.ejps.2024.106868_bib0019","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1136\/gutjnl-2017-314817","article-title":"DNA methylation defines regional identity of human intestinal epithelial organoids and undergoes dynamic changes during development","volume":"68","author":"Kraiczy","year":"2019","journal-title":"Gut"},{"key":"10.1016\/j.ejps.2024.106868_bib0020","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.jcmgh.2019.11.013","article-title":"DNA methylation analysis validates organoids as a viable model for studying human intestinal","volume":"9","author":"Lewis","year":"2020","journal-title":"Aging Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.ejps.2024.106868_bib0021","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.pharmthera.2008.01.006","article-title":"In vitro measurements of metabolism for application in pharmacokinetic modeling","volume":"118","author":"Lipscomb","year":"2008","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.ejps.2024.106868_bib0022","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1124\/dmd.115.068809","article-title":"Human intestinal PEPT1 transporter expression and localization in preterm and term infants","volume":"44","author":"Mooij","year":"2016","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.ejps.2024.106868_bib0023","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1124\/dmd.114.056929","article-title":"Ontogeny of human hepatic and intestinal transporter gene expression during childhood: age matters","volume":"42","author":"Mooij","year":"2014","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.ejps.2024.106868_bib0024","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1002\/bdd.2052","article-title":"Oral drug absorption in pediatrics: the intestinal wall, its developmental changes and current tools for predictions","volume":"38","author":"Nicolas","year":"2017","journal-title":"Biopharm. Drug Dispos."},{"key":"10.1016\/j.ejps.2024.106868_bib0025","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1124\/dmd.106.013037","article-title":"Asymmetric intestinal first-pass metabolism causes minimal oral bioavailability of midazolam in cynomolgus monkey","volume":"35","author":"Nishimura","year":"2007","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.ejps.2024.106868_bib0026","first-page":"1552","article-title":"Characterization of interintestinal and intraintestinal variations in human CYP3A-dependent metabolism","volume":"283","author":"Paine","year":"1997","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ejps.2024.106868_bib0027","first-page":"14","article-title":"First-pass metabolism of midazolam by the human intestine","volume":"60","author":"Paine","year":"1996","journal-title":"Clin. Pharmacol. Ther."},{"key":"10.1016\/j.ejps.2024.106868_bib0028","doi-asserted-by":"crossref","first-page":"272","DOI":"10.3389\/fcimb.2020.00272","article-title":"A human 2D primary organoid-derived epithelial monolayer model to study host-pathogen interaction in the small intestine","volume":"10","author":"Roodsant","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.ejps.2024.106868_bib0029","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1007\/s40262-014-0140-7","article-title":"A Re-evaluation and validation of ontogeny functions for cytochrome P450 1A2 and 3A4 Based on in vivo data","volume":"53","author":"Salem","year":"2014","journal-title":"Clin. Pharmacokinet."},{"key":"10.1016\/j.ejps.2024.106868_bib0030","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1126\/science.1234852","article-title":"Growing self-organizing mini-guts from a single intestinal stem cell: mechanism and applications","volume":"340","author":"Sato","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.ejps.2024.106868_bib0031","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1053\/j.gastro.2011.07.050","article-title":"Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium","volume":"141","author":"Sato","year":"2011","journal-title":"Gastroenterology"},{"key":"10.1016\/j.ejps.2024.106868_bib0032","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.jpedsurg.2016.02.074","article-title":"Long-term renewable human intestinal epithelial stem cells as monolayers: a potential for clinical use","volume":"51","author":"Scott","year":"2016","journal-title":"J. Pediatr. Surg."},{"key":"10.1016\/j.ejps.2024.106868_bib0033","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s13036-019-0165-4","article-title":"Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking","volume":"13","author":"Speer","year":"2019","journal-title":"J. Biol. Eng."},{"key":"10.1016\/j.ejps.2024.106868_bib0034","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s13036-019-0212-1","article-title":"Evaluation of human primary intestinal monolayers for drug metabolizing capabilities","volume":"13","author":"Speer","year":"2019","journal-title":"J. Biol. Eng."},{"key":"10.1016\/j.ejps.2024.106868_bib0035","doi-asserted-by":"crossref","first-page":"2392","DOI":"10.1111\/cts.13368","article-title":"A proof of concept using the Ussing chamber methodology to study pediatric intestinal drug transport and age-dependent differences in absorption","volume":"15","author":"Streekstra","year":"2022","journal-title":"Clin. Transl. Sci."},{"key":"10.1016\/j.ejps.2024.106868_bib0036","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/j.xphs.2015.11.035","article-title":"Application of a human intestinal epithelial cell monolayer to the prediction of oral drug absorption in humans as a superior alternative to the Caco-2 cell monolayer","volume":"105","author":"Takenaka","year":"2016","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.ejps.2024.106868_bib0037","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1002\/jcph.585","article-title":"Meta-analysis of hepatic cytochrome P450 ontogeny to underwrite the prediction of pediatric pharmacokinetics using physiologically based pharmacokinetic modeling","volume":"56","author":"Upreti","year":"2016","journal-title":"J. Clin. Pharmacol."},{"issue":"Suppl 10","key":"10.1016\/j.ejps.2024.106868_bib0038","first-page":"S10","article-title":"Developmental changes in pharmacokinetics and pharmacodynamics","volume":"58","author":"van den Anker","year":"2018","journal-title":"J. Clin. Pharmacol."},{"key":"10.1016\/j.ejps.2024.106868_bib0039","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1002\/cpt.1890","article-title":"The oral bioavailability and metabolism of midazolam in stable critically Ill children: a pharmacokinetic microtracing study","volume":"109","author":"van Groen","year":"2021","journal-title":"Clin. Pharmacol. Ther."},{"key":"10.1016\/j.ejps.2024.106868_bib0040","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1124\/pharmrev.120.000071","article-title":"Ontogeny of hepatic transporters and drug-metabolizing enzymes in humans and in nonclinical species","volume":"73","author":"van Groen","year":"2021","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.ejps.2024.106868_bib0041","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcmgh.2017.02.011","article-title":"Self-renewing monolayer of primary colonic or rectal epithelial cells","volume":"4","author":"Wang","year":"2017","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.ejps.2024.106868_bib0042","doi-asserted-by":"crossref","first-page":"4022","DOI":"10.1021\/jm030999b","article-title":"A physiological model for the estimation of the fraction dose absorbed in humans","volume":"47","author":"Willmann","year":"2004","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ejps.2024.106868_bib0043","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1136\/gut.8.6.618","article-title":"Surface area of the small intestine in man","volume":"8","author":"Wilson","year":"1967","journal-title":"Gut"},{"key":"10.1016\/j.ejps.2024.106868_bib0044","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.omtm.2021.05.005","article-title":"Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research","volume":"22","author":"Yamashita","year":"2021","journal-title":"Mol. Therapy Methods Clin. Dev."}],"container-title":["European Journal of Pharmaceutical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0928098724001805?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0928098724001805?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T23:33:57Z","timestamp":1724715237000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0928098724001805"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":44,"alternative-id":["S0928098724001805"],"URL":"https:\/\/doi.org\/10.1016\/j.ejps.2024.106868","relation":{},"ISSN":["0928-0987"],"issn-type":[{"value":"0928-0987","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The potential of enteroids derived from children and adults to study age-dependent differences in intestinal CYP3A4\/5 metabolism","name":"articletitle","label":"Article Title"},{"value":"European Journal of Pharmaceutical Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejps.2024.106868","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"106868"}}