{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T01:37:07Z","timestamp":1772933827876,"version":"3.50.1"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T00:00:00Z","timestamp":1756944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001661","name":"Universit\u00e4t Heidelberg","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011748","name":"Phospholipid Research Center","doi-asserted-by":"publisher","award":["UMA-2022\\u2013101\/2\\u20131"],"award-info":[{"award-number":["UMA-2022\\u2013101\/2\\u20131"]}],"id":[{"id":"10.13039\/501100011748","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Pharmaceutical Sciences"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.ejps.2025.107258","type":"journal-article","created":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T14:56:03Z","timestamp":1757084163000},"page":"107258","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Single-step, chloroform-free extraction of lysophosphatidylcholine from plasma for cancer biomarker analysis"],"prefix":"10.1016","volume":"214","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1079-5752","authenticated-orcid":false,"given":"Rebecca","family":"Vieth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilena","family":"Wittmaack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birthe","family":"Gericke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gert","family":"Fricker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Massing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejps.2025.107258_bib0001","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1093\/jaoac\/86.2.412","article-title":"Fast and easy multiresidue method employing acetonitrile extraction\/partitioning and \"dispersive solid-phase extraction\" for the determination of pesticide residues in produce","volume":"86","author":"Anastassiades","year":"2003","journal-title":"J. AOAC Int."},{"key":"10.1016\/j.ejps.2025.107258_bib0002","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.chroma.2014.01.024","article-title":"Rapid and simple extraction of lipids from blood plasma and urine for liquid chromatography-tandem mass spectrometry","volume":"1331","author":"Bang","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.ejps.2025.107258_bib0003","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0041456","article-title":"Plasma lysophosphatidylcholine levels are reduced in obesity and type 2 diabetes","volume":"7","author":"Barber","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ejps.2025.107258_bib0004","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/0003-2697(74)90463-1","article-title":"The selective loss of lysophospholipids in some commonly used lipid-extraction procedures","volume":"58","author":"Bjerve","year":"1974","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ejps.2025.107258_bib0005","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"10.1016\/j.ejps.2025.107258_bib0006","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1007\/s10096-011-1505-6","article-title":"Clinical significance of enzymatic lysophosphatidylcholine (LPC) assay data in patients with sepsis","volume":"31","author":"Cho","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ejps.2025.107258_bib0007","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1194\/jlr.M200401-JLR200","article-title":"Plasma ceramide and lysophosphatidylcholine inversely correlate with mortality in sepsis patients","volume":"44","author":"Drobnik","year":"2003","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.ejps.2025.107258_bib0008","unstructured":"European Medicines Agency, ICH M10 on bioanalytical method validation - scientific guideline. https:\/\/www.ema.europa.eu\/en\/ich-m10-bioanalytical-method-validation-scientific-guideline (accessed 3 April 2025)."},{"key":"10.1016\/j.ejps.2025.107258_bib0009","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ejps.2025.107258_bib0010","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/0021-9150(75)90072-6","article-title":"Plasma concentrations of lysolecithin and other phospholipids in the healthy population and in men suffering from atherosclerotic diseases","volume":"22","author":"Gillett","year":"1975","journal-title":"Atherosclerosis"},{"key":"10.1016\/j.ejps.2025.107258_bib0011","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1080\/15476286.2021.2014695","article-title":"Production of human translation-competent lysates using dual centrifugation","volume":"19","author":"Gurzeler","year":"2022","journal-title":"RNA Biol."},{"key":"10.1016\/j.ejps.2025.107258_bib0012","doi-asserted-by":"crossref","first-page":"12292","DOI":"10.1021\/acs.analchem.2c02117","article-title":"Benchmarking one-phase lipid extractions for plasma lipidomics","volume":"94","author":"H\u00f6ring","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ejps.2025.107258_bib0013","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0009-9120(02)00327-2","article-title":"An enzymatic assay for lysophosphatidylcholine concentration in human serum and plasma","volume":"35","author":"Kishimoto","year":"2002","journal-title":"Clin. Biochem."},{"key":"10.1016\/j.ejps.2025.107258_bib0014","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21124501","article-title":"An updated review of pro- and anti-inflammatory properties of plasma lysophosphatidylcholines in the vascular system","volume":"21","author":"Knuplez","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.ejps.2025.107258_bib0015","series-title":"Preparation of Nanosized Pharmaceutical Formulations By Dual Centrifugation","author":"Koehler","year":"2023"},{"key":"10.1016\/j.ejps.2025.107258_bib0016","article-title":"Effects of marine phospholipids extract on the lipid levels of metastatic and nonmetastatic prostate cancer patients","volume":"2014","author":"K\u00fcllenberg de Gaudry","year":"2014","journal-title":"Int. Sch. Res. Notic."},{"key":"10.1016\/j.ejps.2025.107258_bib0017","doi-asserted-by":"crossref","DOI":"10.1016\/j.lfs.2020.117443","article-title":"The mechanisms of lysophosphatidylcholine in the development of diseases","volume":"247","author":"Liu","year":"2020","journal-title":"Life Sci"},{"key":"10.1016\/j.ejps.2025.107258_bib0018","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1194\/jlr.D700041-JLR200","article-title":"Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics","volume":"49","author":"Matyash","year":"2008","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.ejps.2025.107258_bib0019","doi-asserted-by":"crossref","first-page":"e8595","DOI":"10.1002\/rcm.8595","article-title":"Increased plasma phosphatidylcholine\/lysophosphatidylcholine ratios in patients with Parkinson's disease","volume":"34","author":"Mileti\u0107 Vukajlovi\u0107","year":"2020","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.ejps.2025.107258_bib0020","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s12944-015-0070-x","article-title":"Saturated and mono-unsaturated lysophosphatidylcholine metabolism in tumour cells: a potential therapeutic target for preventing metastases","volume":"14","author":"Raynor","year":"2015","journal-title":"Lipids Health Dis."},{"key":"10.1016\/j.ejps.2025.107258_bib0021","doi-asserted-by":"crossref","first-page":"9858","DOI":"10.1021\/ac102211r","article-title":"Quantitative and wide-ranging profiling of phospholipids in human plasma by two-dimensional liquid chromatography\/mass spectrometry","volume":"82","author":"Sato","year":"2010","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ejps.2025.107258_bib0022","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.atherosclerosis.2009.05.029","article-title":"Metabolism and atherogenic disease association of lysophosphatidylcholine","volume":"208","author":"Schmitz","year":"2010","journal-title":"Atherosclerosis"},{"key":"10.1016\/j.ejps.2025.107258_bib0023","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1158\/1055-9965.1185.13.7","article-title":"Lysophospholipids are potential biomarkers of ovarian cancer","volume":"13","author":"Sutphen","year":"2004","journal-title":"Cancer Epidemiol. Biomarkers Prev."},{"key":"10.1016\/j.ejps.2025.107258_bib0024","doi-asserted-by":"crossref","DOI":"10.3390\/ijms25084251","article-title":"Identification of plasma lipid alterations associated with melanoma metastasis","volume":"25","author":"Sz\u00e1sz","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.ejps.2025.107258_bib0025","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s00520-009-0640-4","article-title":"Marine phospholipids\u2014a promising new dietary approach to tumor-associated weight loss","volume":"18","author":"Taylor","year":"2009","journal-title":"Supportive care in cancer"},{"key":"10.1016\/j.ejps.2025.107258_bib0026","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.aca.2018.08.004","article-title":"Optimization of folch, bligh-dyer, and matyash sample-to-extraction solvent ratios for human plasma-based lipidomics studies","volume":"1037","author":"Ulmer","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.ejps.2025.107258_bib0027","doi-asserted-by":"crossref","first-page":"2696","DOI":"10.1200\/JCO.2006.08.5571","article-title":"Plasma lysophosphatidylcholine levels: potential biomarkers for colorectal cancer","volume":"25","author":"Zhao","year":"2007","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.ejps.2025.107258_bib0028","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1194\/jlr.D001503","article-title":"An extremely simple method for extraction of lysophospholipids and phospholipids from blood samples","volume":"51","author":"Zhao","year":"2010","journal-title":"J. Lipid Res."}],"container-title":["European Journal of Pharmaceutical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0928098725002568?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0928098725002568?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T06:52:55Z","timestamp":1772866375000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0928098725002568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":28,"alternative-id":["S0928098725002568"],"URL":"https:\/\/doi.org\/10.1016\/j.ejps.2025.107258","relation":{},"ISSN":["0928-0987"],"issn-type":[{"value":"0928-0987","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-step, chloroform-free extraction of lysophosphatidylcholine from plasma for cancer biomarker analysis","name":"articletitle","label":"Article Title"},{"value":"European Journal of Pharmaceutical Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejps.2025.107258","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"107258"}}