{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T22:24:34Z","timestamp":1793053474189,"version":"4.5.0"},"reference-count":30,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"am","delay-in-days":379,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","ejso.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["European Journal of Surgical Oncology"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1016\/j.ejso.2021.07.017","type":"journal-article","created":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T18:51:10Z","timestamp":1626979870000},"page":"3151-3156","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"title":["Detection of KRAS mutations in circulating tumour DNA from plasma and urine of patients with colorectal cancer"],"prefix":"10.1016","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2627-2859","authenticated-orcid":false,"given":"Ryo","family":"Ohta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1436-7482","authenticated-orcid":false,"given":"Takeshi","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9791-7741","authenticated-orcid":false,"given":"Hiromichi","family":"Sonoda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihisa","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9204-6393","authenticated-orcid":false,"given":"Seiichi","family":"Shinji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goro","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4706-1228","authenticated-orcid":false,"given":"Takuma","family":"Iwai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohki","family":"Takeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7423-5150","authenticated-orcid":false,"given":"Koji","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7055-8032","authenticated-orcid":false,"given":"Sho","family":"Kuriyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4066-0479","authenticated-orcid":false,"given":"Toshimitsu","family":"Miyasaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Yokoyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Hara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ejso.2021.07.017_bib1","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/S1470-2045(10)70130-3","article-title":"Effects of KRAS, BRAF, NRAS, and PIK3CA mutations on the efficacy of cetuximab plus chemotherapy in chemotherapy-refractory metastatic colorectal cancer: a retrospective consortium analysis","volume":"11","author":"De Roock","year":"2010","journal-title":"Lancet Oncol"},{"key":"10.1016\/j.ejso.2021.07.017_bib2","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1056\/NEJMoa1305275","article-title":"Panitumumab-FOLFOX4 treatment and RAS mutations in colorectal cancer","volume":"369","author":"Douillard","year":"2013","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.ejso.2021.07.017_bib3","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1097\/DCR.0b013e31821d37a3","article-title":"Heterogeneity of KRAS status may explain the subset of discordant KRAS status between primary and metastatic colorectal cancer","volume":"54","author":"Watanabe","year":"2011","journal-title":"Dis Colon Rectum"},{"key":"10.1016\/j.ejso.2021.07.017_bib4","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1038\/nm.1789","article-title":"Circulating mutant DNA to assess tumor dynamics","volume":"14","author":"Diehl","year":"2008","journal-title":"Nat Med"},{"key":"10.1016\/j.ejso.2021.07.017_bib5","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1038\/nrclinonc.2017.14","article-title":"Integrating liquid biopsies into the management of cancer","volume":"14","author":"Siravegna","year":"2017","journal-title":"Nat Rev Clin Oncol"},{"key":"10.1016\/j.ejso.2021.07.017_bib6","doi-asserted-by":"crossref","first-page":"3497","DOI":"10.1111\/cas.14186","article-title":"Analysis of colorectal cancer-related mutations by liquid biopsy: utility of circulating cell-free DNA and circulating tumor cells","volume":"110","author":"Takeda","year":"2019","journal-title":"Canc Sci"},{"key":"10.1016\/j.ejso.2021.07.017_bib7","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1038\/s41416-019-0457-y","article-title":"A\u00a0multicentre, prospective study of plasma circulating tumour DNA test for detecting RAS mutation in patients with metastatic colorectal cancer","volume":"120","author":"Bando","year":"2019","journal-title":"Br J Canc"},{"key":"10.1016\/j.ejso.2021.07.017_bib8","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1007\/s10147-020-01691-0","article-title":"Emerging RAS, BRAF, and EGFR mutations in cell-free DNA of metastatic colorectal patients are associated with both primary and secondary resistance to first-line anti-EGFR therapy","volume":"25","author":"Yamada","year":"2020","journal-title":"Int J Clin Oncol"},{"key":"10.1016\/j.ejso.2021.07.017_bib9","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1111\/cas.12959","article-title":"Utility of KRAS mutation detection using circulating cell-free DNA from patients with colorectal cancer","volume":"107","author":"Yamada","year":"2016","journal-title":"Canc Sci"},{"key":"10.1016\/j.ejso.2021.07.017_bib10","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1002\/1878-0261.12023","article-title":"Blood-based detection of RAS mutations to guide anti-EGFR therapy in colorectal cancer patients: concordance of results from circulating tumor DNA and tissue-based RAS testing","volume":"11","author":"Schmiegel","year":"2017","journal-title":"Mol Oncol"},{"key":"10.1016\/j.ejso.2021.07.017_bib11","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1093\/annonc\/mdx112","article-title":"Concordance of blood- and tumor-based detection of RAS mutations to guide anti-EGFR therapy in metastatic colorectal cancer","volume":"28","author":"Grasselli","year":"2017","journal-title":"Ann Oncol"},{"key":"10.1016\/j.ejso.2021.07.017_bib12","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/nrclinonc.2017.166","article-title":"Tumour heterogeneity and resistance to cancer therapies","volume":"15","author":"Dagogo-Jack","year":"2018","journal-title":"Nat Rev Clin Oncol"},{"key":"10.1016\/j.ejso.2021.07.017_bib13","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1038\/s41591-019-0561-9","article-title":"Liquid versus tissue biopsy for detecting acquired resistance and tumor heterogeneity in gastrointestinal cancers","volume":"25","author":"Parikh","year":"2019","journal-title":"Nat Med"},{"key":"10.1016\/j.ejso.2021.07.017_bib14","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1016\/j.ejso.2018.01.224","article-title":"Evaluation of liquid biopsies for detection of emerging mutated genes in metastatic colorectal cancer","volume":"44","author":"Furuki","year":"2018","journal-title":"Eur J Surg Oncol : J Eur Soc Surg Oncol Br Assoc Surg Oncol"},{"key":"10.1016\/j.ejso.2021.07.017_bib15","doi-asserted-by":"crossref","first-page":"346ra92","DOI":"10.1126\/scitranslmed.aaf6219","article-title":"Circulating tumor DNA analysis detects minimal residual disease and predicts recurrence in patients with stage II colon cancer","volume":"8","author":"Tie","year":"2016","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.ejso.2021.07.017_bib16","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1001\/jamaoncol.2019.0528","article-title":"Analysis of plasma cell-free DNA by ultradeep sequencing in patients with stages I to III colorectal cancer","volume":"5","author":"Reinert","year":"2019","journal-title":"JAMA oncology"},{"key":"10.1016\/j.ejso.2021.07.017_bib17","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/nrc3066","article-title":"Cell-free nucleic acids as biomarkers in cancer patients","volume":"11","author":"Schwarzenbach","year":"2011","journal-title":"Nat Rev Canc"},{"key":"10.1016\/j.ejso.2021.07.017_bib18","doi-asserted-by":"crossref","DOI":"10.1136\/esmoopen-2019-000572","article-title":"Whole exome sequencing analysis of urine trans-renal tumour DNA in metastatic colorectal cancer patients","volume":"4","author":"Crisafulli","year":"2019","journal-title":"ESMO open"},{"key":"10.1016\/j.ejso.2021.07.017_bib19","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1196\/annals.1448.027","article-title":"Detection of mutated K-ras DNA in urine, plasma, and serum of patients with colorectal carcinoma or adenomatous polyps","volume":"1137","author":"Su","year":"2008","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.ejso.2021.07.017_bib20","doi-asserted-by":"crossref","first-page":"3657","DOI":"10.1158\/1078-0432.CCR-16-2592","article-title":"Mutation-enrichment next-generation sequencing for quantitative detection of KRAS mutations in urine cell-free DNA from patients with advanced cancers","volume":"23","author":"Fujii","year":"2017","journal-title":"Clin Canc Res : Off J Am Assoc Canc Res"},{"key":"10.1016\/j.ejso.2021.07.017_bib21","first-page":"35","article-title":"Microsatellite analysis of free tumor DNA in urine, serum, and plasma of patients: a minimally invasive method for the detection of bladder cancer","volume":"8","author":"Utting","year":"2002","journal-title":"Clin Canc Res : Off J Am Assoc Canc Res"},{"key":"10.1016\/j.ejso.2021.07.017_bib22","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1126\/science.2024123","article-title":"Identification of p53 gene mutations in bladder cancers and urine samples","volume":"252","author":"Sidransky","year":"1991","journal-title":"Science (New York, NY)"},{"key":"10.1016\/j.ejso.2021.07.017_bib23","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1080\/15384047.2019.1638685","article-title":"Utility of liquid biopsy using urine in patients with pancreatic ductal adenocarcinoma","volume":"20","author":"Terasawa","year":"2019","journal-title":"Canc Biol Ther"},{"key":"10.1016\/j.ejso.2021.07.017_bib24","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1016\/j.jtho.2016.05.035","article-title":"A\u00a0highly sensitive and quantitative test platform for detection of NSCLC EGFR mutations in urine and plasma","volume":"11","author":"Reckamp","year":"2016","journal-title":"J\u00a0Thorac Oncol"},{"key":"10.1016\/j.ejso.2021.07.017_bib25","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/j.eururo.2016.12.016","article-title":"Liquid biopsy analysis of FGFR3 and PIK3CA hotspot mutations for disease surveillance in bladder cancer","volume":"71","author":"Christensen","year":"2017","journal-title":"Eur Urol"},{"key":"10.1016\/j.ejso.2021.07.017_bib26","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1002\/cam4.1935","article-title":"Differences in the genomic profiles of cell-free DNA between plasma, sputum, urine, and tumor tissue in advanced NSCLC","volume":"8","author":"Wu","year":"2019","journal-title":"Cancer Med"},{"key":"10.1016\/j.ejso.2021.07.017_bib27","doi-asserted-by":"crossref","first-page":"2984","DOI":"10.1002\/ijc.28946","article-title":"Cell-free DNA in healthy individuals, noncancerous disease and strong prognostic value in colorectal cancer","volume":"135","author":"Spindler","year":"2014","journal-title":"Int J Cancer J Int Cancer"},{"key":"10.1016\/j.ejso.2021.07.017_bib28","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.molonc.2014.02.005","article-title":"Multi-marker analysis of circulating cell-free DNA toward personalized medicine for colorectal cancer","volume":"8","author":"Mouliere","year":"2014","journal-title":"Mol Oncol"},{"key":"10.1016\/j.ejso.2021.07.017_bib29","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aan2415","article-title":"Direct detection of early-stage cancers using circulating tumor DNA","volume":"9","author":"Phallen","year":"2017","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.ejso.2021.07.017_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0146275","article-title":"Individualized mutation detection in circulating tumor DNA for monitoring colorectal tumor burden using a cancer-associated gene sequencing panel","volume":"11","author":"Sato","year":"2016","journal-title":"PloS One"}],"container-title":["European Journal of Surgical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0748798321006387?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0748798321006387?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:40:03Z","timestamp":1760060403000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0748798321006387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["S0748798321006387"],"URL":"https:\/\/doi.org\/10.1016\/j.ejso.2021.07.017","relation":{},"ISSN":["0748-7983"],"issn-type":[{"value":"0748-7983","type":"print"}],"subject":[],"published":{"date-parts":[[2021,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection of KRAS mutations in circulating tumour DNA from plasma and urine of patients with colorectal cancer","name":"articletitle","label":"Article Title"},{"value":"European Journal of Surgical Oncology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ejso.2021.07.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Ltd, BASO ~ The Association for Cancer Surgery, and the European Society of Surgical Oncology. All rights reserved.","name":"copyright","label":"Copyright"}]}}