{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T21:13:07Z","timestamp":1788988387082,"version":"build-2803163510"},"reference-count":74,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Shanghai Municipality Science and Technology Commission","doi-asserted-by":"publisher","award":["24HC2810200"],"award-info":[{"award-number":["24HC2810200"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Engineering Microbiology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.engmic.2026.100302","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T14:52:00Z","timestamp":1787410320000},"page":"100302","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["An efficient endogenous type I-E CRISPR-Cas genome-editing platform for producing transglutaminase in Streptomyces mobaraensis"],"prefix":"10.1016","volume":"6","author":[{"given":"Dan","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxu","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianjin","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3269-9747","authenticated-orcid":false,"given":"Linquan","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"10","key":"10.1016\/j.engmic.2026.100302_bib0001","first-page":"2613","article-title":"Purification and characteristics of a novel transglutaminase derived from microorganisms","volume":"53","author":"Ando","year":"1989","journal-title":"Agri Biol Chem."},{"key":"10.1016\/j.engmic.2026.100302_bib0002","first-page":"655","article-title":"Production of microbial transglutaminase by Streptoverticillium cinnamoneum KKP 1658","volume":"23","author":"Kolotylo","year":"2024","journal-title":"EXCLI. J."},{"issue":"1","key":"10.1016\/j.engmic.2026.100302_bib0003","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s11274-019-2791-x","article-title":"Transglutaminases: part I-origins, sources, and biotechnological characteristics","volume":"36","author":"Duarte","year":"2020","journal-title":"World J. Microb. Biot."},{"key":"10.1016\/j.engmic.2026.100302_bib0004","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.micres.2018.06.001","article-title":"Current insight and futuristic vistas of microbial transglutaminase in nutraceutical industry","volume":"215","author":"Fatima","year":"2018","journal-title":"Microbiol. Res."},{"issue":"4","key":"10.1016\/j.engmic.2026.100302_bib0005","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/s00253-003-1539-5","article-title":"Properties and applications of microbial transglutaminase","volume":"64","author":"Yokoyama","year":"2004","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.engmic.2026.100302_bib0006","first-page":"11","article-title":"Biocatalysis by transglutaminases: a review of biotechnological applications","volume":"9","author":"Savoca","year":"2018","journal-title":"Micromachines-Basel."},{"issue":"7","key":"10.1016\/j.engmic.2026.100302_bib0007","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1007\/s00253-019-09669-3","article-title":"Bioengineering of microbial transglutaminase for biomedical applications","volume":"103","author":"Chan","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"7","key":"10.1016\/j.engmic.2026.100302_bib0008","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1016\/j.enzmictec.2006.12.001","article-title":"Improvement of shrink-resistance and tensile strength of wool fabric treated with a novel microbial transglutaminase from Streptomyces hygroscopicus","volume":"40","author":"Du","year":"2007","journal-title":"Enzyme Microb. Technol."},{"issue":"1","key":"10.1016\/j.engmic.2026.100302_bib0009","article-title":"Microbiological transglutaminase: biotechnological application in the food industry","volume":"18","author":"Kolotylo","year":"2023","journal-title":"Open. Life Sci."},{"issue":"1","key":"10.1016\/j.engmic.2026.100302_bib0010","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s11274-019-2792-9","article-title":"Review transglutaminases: part II-industrial applications in food, biotechnology, textiles and leather products","volume":"36","author":"Duarte","year":"2020","journal-title":"World J. Microb. Biot."},{"issue":"1","key":"10.1016\/j.engmic.2026.100302_bib0011","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/s00253-025-13697-7","article-title":"Microbial transglutaminase in food biotechnology: from biochemical mechanisms to industrial applications","volume":"110","author":"Kieliszek","year":"2026","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.engmic.2026.100302_bib0012","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2025.1525673","article-title":"Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategies","volume":"16","author":"Chang","year":"2025","journal-title":"Front. Microbiol."},{"issue":"1","key":"10.1016\/j.engmic.2026.100302_bib0013","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s40643-017-0168-2","article-title":"Enhancing transglutaminase production of Streptomyces mobaraensis by iterative mutagenesis breeding with atmospheric and room-temperature plasma (ARTP)","volume":"4","author":"Jiang","year":"2017","journal-title":"Bioresour. Bioprocess."},{"key":"10.1016\/j.engmic.2026.100302_bib0014","first-page":"216","article-title":"Screening and statistical optimization of media ingredients for production of microbial transglutaminase","volume":"2","author":"Xavier","year":"2017","journal-title":"Life Sci. J."},{"issue":"6","key":"10.1016\/j.engmic.2026.100302_bib0015","doi-asserted-by":"crossref","first-page":"2335","DOI":"10.1007\/s00253-011-3790-5","article-title":"Enhancement of transglutaminase production in Streptomyces mobaraensis as achieved by treatment with excessive MgCl2","volume":"93","author":"Zhang","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"5","key":"10.1016\/j.engmic.2026.100302_bib0016","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1007\/s12010-013-0251-x","article-title":"Two-staged temperature and agitation strategy for the production of transglutaminase from a Streptomyces sp. isolated from Brazilian soils","volume":"170","author":"Bagagli","year":"2013","journal-title":"Appl. Biochem. Biotechnol."},{"issue":"4","key":"10.1016\/j.engmic.2026.100302_bib0017","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0141-0229(02)00127-8","article-title":"pH control strategy of batch microbial transglutaminase production with Streptoverticillium mobaraense","volume":"31","author":"Z","year":"2002","journal-title":"Enzyme Microb. Technol."},{"issue":"7","key":"10.1016\/j.engmic.2026.100302_bib0018","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1007\/s11814-011-0274-3","article-title":"Enhancement of transglutaminase production in Streptomyces mobaraensis DSM 40587 by non-nutritional stress conditions: effects of heat shock, alcohols, and salt treatments","volume":"29","author":"Zhang","year":"2012","journal-title":"Korean J. Chem. Eng."},{"issue":"8","key":"10.1016\/j.engmic.2026.100302_bib0019","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1023\/A:1020472908615","article-title":"Modelling of temperature effects on batch microbial transglutaminase fermentation with Streptoverticillium mobaraense","volume":"18","author":"Zheng","year":"2002","journal-title":"World J. Microb. Biot."},{"issue":"4","key":"10.1016\/j.engmic.2026.100302_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.engmic.2023.100098","article-title":"Identification of multiple regulatory genes involved in TGase production in Streptomyces mobaraensis DSM 40587","volume":"3","author":"Liu","year":"2023","journal-title":"Eng. Microbiol."},{"key":"10.1016\/j.engmic.2026.100302_bib0021","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2024.1397314","article-title":"LexA, an SOS response repressor, activates TGase synthesis in Streptomyces mobaraensis","volume":"15","author":"Shi","year":"2024","journal-title":"Front. Microbiol."},{"issue":"5","key":"10.1016\/j.engmic.2026.100302_bib0022","doi-asserted-by":"crossref","first-page":"687","DOI":"10.3136\/fstr.25.687","article-title":"Molecular cloning and overexpression of the transglutaminase gene from Streptomyces mobaraensis","volume":"25","author":"Xue","year":"2019","journal-title":"Food Sci Technol Res."},{"key":"10.1016\/j.engmic.2026.100302_bib0023","doi-asserted-by":"crossref","DOI":"10.1016\/j.crfs.2024.100756","article-title":"Efficient biosynthesis of transglutaminase in Streptomyces mobaraensis via systematic engineering strategies","volume":"8","author":"Yuan","year":"2024","journal-title":"Curr. Res. Food Sci."},{"key":"10.1016\/j.engmic.2026.100302_bib0024","doi-asserted-by":"crossref","first-page":"600","DOI":"10.3390\/antibiotics11050600","article-title":"Comparative transcriptome-based mining of genes involved in the export of polyether antibiotics for titer improvement","volume":"11","author":"Liu","year":"2022","journal-title":"Antibiotics."},{"key":"10.1016\/j.engmic.2026.100302_bib0025","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/978-1-62703-299-5_22","article-title":"Comparative transcriptome analysis for metabolic engineering","volume":"985","author":"Shi","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.engmic.2026.100302_bib0026","first-page":"7","article-title":"Enhanced \u03b5-poly-l-lysine production in Streptomyces albulus through multi-omics-guided metabolic engineering","volume":"14","author":"Wang","year":"2024","journal-title":"Biomolecules."},{"key":"10.1016\/j.engmic.2026.100302_bib0027","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodres.2022.112275","article-title":"Integrated metabolomics and transcriptomics reveal metabolites difference between wild and cultivated Ophiocordyceps sinensis","volume":"163","author":"Zhang","year":"2023","journal-title":"Food Res. Int."},{"issue":"7","key":"10.1016\/j.engmic.2026.100302_bib0028","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1007\/s10529-008-9671-z","article-title":"Application of conjugation using phiC31 att\/int system for Actinoplanes teichomyceticus, a producer of teicoplanin","volume":"30","author":"Ha","year":"2008","journal-title":"Biotechnol. Lett."},{"issue":"9","key":"10.1016\/j.engmic.2026.100302_bib0029","doi-asserted-by":"crossref","DOI":"10.1002\/biot.201800121","article-title":"CRISPR\/dCas9-mediated multiplex gene repression in Streptomyces","volume":"13","author":"Zhao","year":"2018","journal-title":"Biotechnol. J."},{"issue":"6","key":"10.1016\/j.engmic.2026.100302_bib0030","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1021\/acs.orglett.7b00283","article-title":"Genome mining of Streptomyces mobaraensis DSM 40847 as a bleomycin producer providing a biotechnology platform to engineer designer bleomycin analogues","volume":"19","author":"Hindra","year":"2017","journal-title":"Org. Lett."},{"issue":"4","key":"10.1016\/j.engmic.2026.100302_bib0031","doi-asserted-by":"crossref","first-page":"678","DOI":"10.4014\/jmb.0910.10031","article-title":"Two pHZ1358-derivative vectors for efficient gene knockout in Streptomyces","volume":"20","author":"He","year":"2010","journal-title":"J. Microbiol. Biotechnol."},{"issue":"9","key":"10.1016\/j.engmic.2026.100302_bib0032","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1021\/acssynbio.5b00038","article-title":"CRISPR-Cas9 based engineering of actinomycetal genomes","volume":"4","author":"Tong","year":"2015","journal-title":"ACS. Synth. Biol."},{"issue":"18","key":"10.1016\/j.engmic.2026.100302_bib0033","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.00827-18","article-title":"CRISPR-Cpf1-assisted multiplex genome editing and transcriptional repression in Streptomyces","volume":"84","author":"Li","year":"2018","journal-title":"Appl. Env. Microbiol."},{"issue":"9","key":"10.1016\/j.engmic.2026.100302_bib0034","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1039\/C9NP00029A","article-title":"Challenges and advances in genetic manipulation of filamentous actinomycetes\u2013the remarkable producers of specialized metabolites","volume":"36","author":"Musiol-Kroll","year":"2019","journal-title":"Nat. Prod. Rep."},{"issue":"5","key":"10.1016\/j.engmic.2026.100302_bib0035","doi-asserted-by":"crossref","first-page":"734","DOI":"10.3390\/biom10050734","article-title":"Challenges and advances in genome editing technologies in Streptomyces","volume":"10","author":"Zhao","year":"2020","journal-title":"Biomolecules."},{"issue":"12","key":"10.1016\/j.engmic.2026.100302_bib0036","doi-asserted-by":"crossref","first-page":"3346","DOI":"10.1038\/s41564-025-02180-8","article-title":"An updated evolutionary classification of CRISPR-Cas systems including rare variants","volume":"10","author":"Makarova","year":"2025","journal-title":"Nat. Microbiol."},{"issue":"5","key":"10.1016\/j.engmic.2026.100302_bib0037","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1007\/s11427-024-2869-x","article-title":"Developing a robust genome editing tool based on an endogenous type I-B CRISPR-Cas system in Saccharopolyspora spinosa","volume":"68","author":"Wang","year":"2025","journal-title":"Sci. China Life Sci."},{"issue":"32","key":"10.1016\/j.engmic.2026.100302_bib0038","doi-asserted-by":"crossref","first-page":"15774","DOI":"10.1073\/pnas.1905421116","article-title":"Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus","volume":"116","author":"Hidalgo-Cantabrana","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U S. A"},{"issue":"4","key":"10.1016\/j.engmic.2026.100302_bib0039","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1007\/s11427-024-2677-4","article-title":"Harnessing the Streptomyces-originating type I-E CRISPR\/Cas system for efficient genome editing in Streptomyces","volume":"68","author":"Xie","year":"2025","journal-title":"Sci. China Life Sci."},{"issue":"4","key":"10.1016\/j.engmic.2026.100302_bib0040","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkv1044","article-title":"Harnessing type I and type III CRISPR-Cas systems for genome editing","volume":"44","author":"Li","year":"2016","journal-title":"Nucleic. Acids. Res."},{"issue":"6","key":"10.1016\/j.engmic.2026.100302_bib0041","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaf214","article-title":"Cascade-Cas3 enables highly efficient genome engineering in Streptomyces species","volume":"53","author":"Whitford","year":"2025","journal-title":"Nucleic. Acids. Res."},{"key":"10.1016\/j.engmic.2026.100302_bib0042","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.3389\/fmicb.2019.02471","article-title":"Endogenous CRISPR-Cas system-based genome editing and antimicrobials: review and prospects","volume":"10","author":"Li","year":"2019","journal-title":"Front. Microbiol."},{"issue":"3","key":"10.1016\/j.engmic.2026.100302_bib0043","article-title":"Harnessing the endogenous type I-C CRISPR-Cas system for genome editing in Bifidobacterium breve","volume":"90","author":"Han","year":"2024","journal-title":"Appl Env. Microbiol."},{"issue":"11","key":"10.1016\/j.engmic.2026.100302_bib0044","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/j.jgg.2017.09.010","article-title":"Harnessing the native type I-B CRISPR-Cas for genome editing in a polyploid archaeon","volume":"44","author":"Cheng","year":"2017","journal-title":"J Genet Genom."},{"issue":"2","key":"10.1016\/j.engmic.2026.100302_bib0045","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1111\/1462-2920.15116","article-title":"Harnessing the type I CRISPR-Cas systems for genome editing in prokaryotes","volume":"23","author":"Xu","year":"2021","journal-title":"Env. Microbiol."},{"key":"10.1016\/j.engmic.2026.100302_bib0046","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1128\/spectrum.02786-25","article-title":"Unlocking genome engineering in Alcaligenes faecalis by exploiting its native type I-F CRISPR-Cas","volume":"14","author":"Cheng","year":"2026","journal-title":"Microbiol. Spectr."},{"issue":"1","key":"10.1016\/j.engmic.2026.100302_bib0047","doi-asserted-by":"crossref","first-page":"9833","DOI":"10.1038\/s41467-024-54196-z","article-title":"Repurposing endogenous type I-E CRISPR-Cas systems for natural product discovery in Streptomyces","volume":"15","author":"Zhou","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.engmic.2026.100302_bib0048","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2023.108241","article-title":"Endogenous CRISPR-Cas mediated in situ genome editing: state-of-the-art and the road ahead for engineering prokaryotes","volume":"68","author":"Liu","year":"2023","journal-title":"Biotechnol. Adv."},{"issue":"W1","key":"10.1016\/j.engmic.2026.100302_bib0049","doi-asserted-by":"crossref","first-page":"W246","DOI":"10.1093\/nar\/gky425","article-title":"CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins","volume":"46","author":"Couvin","year":"2018","journal-title":"Nucleic. Acids. Res."},{"issue":"5","key":"10.1016\/j.engmic.2026.100302_bib0050","doi-asserted-by":"crossref","first-page":"817","DOI":"10.4161\/rna.24046","article-title":"CRISPRTarget: bioinformatic prediction and analysis of crRNA targets","volume":"10","author":"Biswas","year":"2013","journal-title":"RNa Biol."},{"issue":"6","key":"10.1016\/j.engmic.2026.100302_bib0051","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1101\/gr.849004","article-title":"WebLogo: a sequence logo generator","volume":"14","author":"Crooks","year":"2004","journal-title":"Genome Res."},{"key":"10.1016\/j.engmic.2026.100302_bib0052","series-title":"Practical Streptomyces genetics","author":"Kieser","year":"2000"},{"issue":"5","key":"10.1016\/j.engmic.2026.100302_bib0053","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1007\/s11274-012-1017-2","article-title":"Hydroxamate-based colorimetric method for direct screening of transglutaminase-producing bacteria","volume":"28","author":"Bourneow","year":"2012","journal-title":"World J. Microb. Biot."},{"issue":"9","key":"10.1016\/j.engmic.2026.100302_bib0054","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1039\/D4NP00010B","article-title":"Unleashing the potential: type I CRISPR-Cas systems in actinomycetes for genome editing","volume":"41","author":"Wang","year":"2024","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.engmic.2026.100302_bib0055","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.ymben.2018.03.007","article-title":"Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production","volume":"47","author":"Zhang","year":"2018","journal-title":"Metab. Eng."},{"issue":"1","key":"10.1016\/j.engmic.2026.100302_bib0056","doi-asserted-by":"crossref","first-page":"5302","DOI":"10.1038\/s41467-019-13226-x","article-title":"CRISPR-Cas3 induces broad and unidirectional genome editing in human cells","volume":"10","author":"Morisaka","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.engmic.2026.100302_bib0057","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1128\/ecosalplus.esp-0008-2018","article-title":"Mechanisms of type I-E and I-F CRISPR-Cas systems in Enterobacteriaceae","volume":"8","author":"Xue","year":"2019","journal-title":"EcoSal. Plus."},{"issue":"3","key":"10.1016\/j.engmic.2026.100302_bib0058","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1093\/femsre\/fuv023","article-title":"Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity","volume":"39","author":"Charpentier","year":"2015","journal-title":"FEMS Microbiol. Rev."},{"issue":"7542","key":"10.1016\/j.engmic.2026.100302_bib0059","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/nature14245","article-title":"Cas9 specifies functional viral targets during CRISPR-Cas adaptation","volume":"519","author":"Heler","year":"2015","journal-title":"Nature"},{"issue":"3","key":"10.1016\/j.engmic.2026.100302_bib0060","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1007\/s11427-021-2165-1","article-title":"Highly efficient base editing in rabbit by using near-PAMless engineered CRISPR\/Cas9 variants","volume":"66","author":"Zhang","year":"2023","journal-title":"Sci. China Life Sci."},{"issue":"1","key":"10.1016\/j.engmic.2026.100302_bib0061","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ja.2005.1","article-title":"Bioactive microbial metabolites","volume":"58","author":"Berdy","year":"2005","journal-title":"J. Antibiot. (Tokyo)"},{"issue":"7","key":"10.1016\/j.engmic.2026.100302_bib0062","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1016\/j.cell.2017.05.042","article-title":"Antibacterial nucleoside-analog inhibitor of bacterial RNA polymerase","volume":"169","author":"Maffioli","year":"2017","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.engmic.2026.100302_bib0063","doi-asserted-by":"crossref","first-page":"5261","DOI":"10.1038\/s41467-020-18950-3","article-title":"Structure of inhibitor-bound mammalian complex I","volume":"11","author":"Bridges","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.engmic.2026.100302_bib0064","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1128\/mBio.01714-15","article-title":"In vitro CRISPR\/Cas9 system for efficient targeted DNA editing","volume":"6","author":"Liu","year":"2015","journal-title":"mBio"},{"issue":"9","key":"10.1016\/j.engmic.2026.100302_bib0065","doi-asserted-by":"crossref","first-page":"2330","DOI":"10.1002\/bit.27021","article-title":"Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes","volume":"116","author":"Yeo","year":"2019","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.engmic.2026.100302_bib0066","article-title":"CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters","author":"Zhang","year":"2017","journal-title":"Nat. Chem. Biol."},{"issue":"4\u20135","key":"10.1016\/j.engmic.2026.100302_bib0067","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1007\/s10295-020-02277-5","article-title":"Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces","volume":"47","author":"Ye","year":"2020","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"10.1016\/j.engmic.2026.100302_bib0068","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.3389\/fmicb.2017.01851","article-title":"Characterization and exploitation of CRISPR loci in Bifidobacterium longum","volume":"8","author":"Hidalgo-Cantabrana","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.engmic.2026.100302_bib0069","doi-asserted-by":"crossref","first-page":"3716","DOI":"10.1021\/acs.jafc.8b00383","article-title":"A comparative proteome approach reveals metabolic changes associated with Flammulina velutipes mycelia in response to cold and light stress","volume":"66","author":"Liu","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.engmic.2026.100302_bib0070","doi-asserted-by":"crossref","first-page":"396","DOI":"10.3389\/fmicb.2020.00396","article-title":"Deciphering the metabolic pathway difference between Saccharopolyspora pogona and Saccharopolyspora spinosa by comparative proteomics and metabonomics","volume":"11","author":"Rang","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.engmic.2026.100302_bib0071","doi-asserted-by":"crossref","first-page":"12554","DOI":"10.1021\/acs.jafc.1c03654","article-title":"Comparative proteomics reveals the effect of the transcriptional regulator Sp13016 on butenyl-spinosyn biosynthesis in Saccharopolyspora pogona","volume":"69","author":"Tang","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.engmic.2026.100302_bib0072","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1475-2859-13-27","article-title":"Differential proteomic profiling reveals regulatory proteins and novel links between primary metabolism and spinosad production in Saccharopolyspora spinosa","volume":"13","author":"Yang","year":"2014","journal-title":"Microb. Cell Fact."},{"issue":"7","key":"10.1016\/j.engmic.2026.100302_bib0073","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1038\/emboj.2011.41","article-title":"Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR\/Cas immune system","volume":"30","author":"Sinkunas","year":"2011","journal-title":"EMBO J."},{"issue":"6","key":"10.1016\/j.engmic.2026.100302_bib0074","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1016\/j.gpb.2022.12.003","article-title":"Superior fidelity and distinct editing outcomes of SaCas9 compared with SpCas9 in genome editing","volume":"21","author":"Yang","year":"2023","journal-title":"Genom Proteom Bioinf."}],"container-title":["Engineering Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2667370326000433?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2667370326000433?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T20:53:47Z","timestamp":1788987227000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2667370326000433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":74,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["S2667370326000433"],"URL":"https:\/\/doi.org\/10.1016\/j.engmic.2026.100302","relation":{},"ISSN":["2667-3703"],"issn-type":[{"value":"2667-3703","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An efficient endogenous type I-E CRISPR-Cas genome-editing platform for producing transglutaminase in Streptomyces mobaraensis","name":"articletitle","label":"Article Title"},{"value":"Engineering Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.engmic.2026.100302","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2026 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.","name":"copyright","label":"Copyright"}],"article-number":"100302"}}