{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:14:03Z","timestamp":1792120443991,"version":"4.4.0"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Environment International"],"published-print":{"date-parts":[[2024,5]]},"DOI":"10.1016\/j.envint.2024.108717","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T08:45:08Z","timestamp":1714985108000},"page":"108717","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["Do PFCAs drive the establishment of thyroid cancer microenvironment? Effects of C6O4, PFOA and PFHxA exposure in two models of human thyroid cells in primary culture"],"prefix":"10.1016","volume":"187","author":[{"given":"Francesca","family":"Coperchini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Greco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Croce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4606-8923","authenticated-orcid":false,"given":"Marsida","family":"Teliti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedetto","family":"Cal\u00ec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spyridon","family":"Chytiris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Magri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Rotondi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.envint.2024.108717_b0005","first-page":"11","article-title":"Per- and Polyfluoroalkyl substances (PFAS) in community water systems (cws) and the risk of thyroid cancer: an ecological study","author":"Alsen","year":"2023","journal-title":"Toxics"},{"key":"10.1016\/j.envint.2024.108717_b0010","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1089\/thy.2013.0584","article-title":"Re: Brito et al., overdiagnosis of thyroid cancer and Graves' disease","volume":"24","author":"Aschebrook-Kilfoy","year":"2014","journal-title":"Thyroid"},{"key":"10.1016\/j.envint.2024.108717_b0015","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/978-3-030-62658-7_3","article-title":"CXCL8 signaling in the tumor microenvironment","volume":"1302","author":"Asokan","year":"2021","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.envint.2024.108717_b0020","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1289\/ehp.1306615","article-title":"Perfluorooctanoic acid (PFOA) exposures and incident cancers among adults living near a chemical plant","volume":"121","author":"Barry","year":"2013","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.envint.2024.108717_b0025","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. Clin."},{"key":"10.1016\/j.envint.2024.108717_b0030","doi-asserted-by":"crossref","first-page":"4667","DOI":"10.2147\/OTT.S209734","article-title":"Effect of overexpression of Oct4 and Sox2 genes on the biological and oncological characteristics of gastric cancer cells","volume":"12","author":"Chen","year":"2019","journal-title":"Onco Targets Ther"},{"key":"10.1016\/j.envint.2024.108717_b0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2020.106204","article-title":"Hexafluoropropylene oxide-dimer acid (HFPO-DA or GenX) alters maternal and fetal glucose and lipid metabolism and produces neonatal mortality, low birthweight, and hepatomegaly in the Sprague-Dawley rat","volume":"146","author":"Conley","year":"2021","journal-title":"Environ. Int."},{"key":"10.1016\/j.envint.2024.108717_b0040","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1007\/s11356-014-3480-9","article-title":"Exposure to perfluorinated compounds: in vitro study on thyroid cells","volume":"22","author":"Coperchini","year":"2015","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.envint.2024.108717_b0045","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s40618-016-0572-z","article-title":"Thyroid disruption by perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA)","volume":"40","author":"Coperchini","year":"2017","journal-title":"J. Endocrinol. Invest."},{"key":"10.1016\/j.envint.2024.108717_b0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2020.114778","article-title":"Adverse effects of in vitro GenX exposure on rat thyroid cell viability, DNA integrity and thyroid-related genes expression","volume":"264","author":"Coperchini","year":"2020","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.envint.2024.108717_b0055","article-title":"Thyroid disrupting effects of old and new generation PFAS","volume":"11","author":"Coperchini","year":"2020","journal-title":"Front Endocrinol (lausanne)"},{"key":"10.1016\/j.envint.2024.108717_b0060","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1007\/s40618-020-01466-4","article-title":"The new generation PFAS C6O4 does not produce adverse effects on thyroid cells in vitro","volume":"44","author":"Coperchini","year":"2021","journal-title":"J. Endocrinol. Invest."},{"key":"10.1016\/j.envint.2024.108717_b0065","doi-asserted-by":"crossref","first-page":"63522","DOI":"10.1007\/s11356-023-26797-6","article-title":"PFOA, PFHxA and C6O4 differently modulate the expression of CXCL8 in normal thyroid cells and in thyroid cancer cell lines","volume":"30","author":"Coperchini","year":"2023","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.envint.2024.108717_b0070","doi-asserted-by":"crossref","DOI":"10.1007\/s40618-019-01062-1","article-title":"Effect of long- and short-chain perfluorinated compounds on cultured thyroid cells viability and response to TSH","author":"Croce","year":"2019","journal-title":"J. Endocrinol. Invest."},{"key":"10.1016\/j.envint.2024.108717_b0075","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1007\/s40618-019-01062-1","article-title":"Effect of long- and short-chain perfluorinated compounds on cultured thyroid cells viability and response to TSH","volume":"42","author":"Croce","year":"2019","journal-title":"J. Endocrinol. Invest."},{"key":"10.1016\/j.envint.2024.108717_b0080","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1002\/bem.20649","article-title":"Electromagnetic exposure of scaffold-free three-dimensional cell culture systems","volume":"32","author":"Daus","year":"2011","journal-title":"Bioelectromagnetics"},{"key":"10.1016\/j.envint.2024.108717_b0085","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1001\/jama.295.18.2164","article-title":"Increasing incidence of thyroid cancer in the United States, 1973\u20132002","volume":"295","author":"Davies","year":"2006","journal-title":"J. Am. Med. Assoc."},{"key":"10.1016\/j.envint.2024.108717_b0090","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2022.915096","article-title":"Comparative Evaluation of the effects of Legacy and New Generation Perfluoralkyl Substances (PFAS) on Thyroid Cells","volume":"13","author":"De Toni","year":"2022","journal-title":"Front Endocrinol (lausanne)"},{"key":"10.1016\/j.envint.2024.108717_b0095","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.771210","article-title":"Targeting the CCL2\/CCR2 Axis in Cancer Immunotherapy: One Stone, Three Birds?","volume":"12","author":"Fei","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.envint.2024.108717_b0100","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1093\/toxsci\/kfs122","article-title":"Determination of drug toxicity using 3D spheroids constructed from an immortal human hepatocyte cell line","volume":"127","author":"Fey","year":"2012","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.envint.2024.108717_b0105","doi-asserted-by":"crossref","first-page":"2738","DOI":"10.1002\/ijc.29311","article-title":"Thyroid cancer: an epidemic of disease or an epidemic of diagnosis?","volume":"136","author":"Franceschi","year":"2015","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.envint.2024.108717_b0110","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1002\/cac2.12416","article-title":"Tumor microenvironment signaling and therapeutics in cancer progression","volume":"43","author":"Goenka","year":"2023","journal-title":"Cancer Commun (lond)"},{"key":"10.1016\/j.envint.2024.108717_b0115","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1001\/jamaoto.2022.3360","article-title":"American thyroid association guidelines and national trends in management of papillary thyroid carcinoma","volume":"148","author":"Gordon","year":"2022","journal-title":"JAMA Otolaryngol. Head Neck Surg."},{"key":"10.1016\/j.envint.2024.108717_b0120","doi-asserted-by":"crossref","DOI":"10.3390\/molecules27010137","article-title":"Roles of the CXCL8-CXCR1\/2 axis in the tumor microenvironment and immunotherapy","volume":"27","author":"Han","year":"2021","journal-title":"Molecules"},{"key":"10.1016\/j.envint.2024.108717_b0125","doi-asserted-by":"crossref","DOI":"10.3390\/ijms22168530","article-title":"Roles of CCL2-CCR2 axis in the tumor microenvironment","volume":"22","author":"Kadomoto","year":"2021","journal-title":"Int. J. Mol. Sci."},{"issue":"83","key":"10.1016\/j.envint.2024.108717_b9000","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1002\/bit.10655","article-title":"Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types","volume":"2003","author":"Kelm","year":"2003","journal-title":"Biotechnol Bioeng."},{"key":"10.1016\/j.envint.2024.108717_b0130","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1038\/nrendo.2016.110","article-title":"The changing incidence of thyroid cancer","volume":"12","author":"Kitahara","year":"2016","journal-title":"Nat. Rev. Endocrinol."},{"key":"10.1016\/j.envint.2024.108717_b0135","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1038\/nrm3758","article-title":"Molecular mechanisms of epithelial-mesenchymal transition","volume":"15","author":"Lamouille","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.envint.2024.108717_b0140","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.actbio.2018.08.008","article-title":"Multicellular spheroid based on a triple co-culture: a novel 3D model to mimic pancreatic tumor complexity","volume":"78","author":"Lazzari","year":"2018","journal-title":"Acta Biomater."},{"key":"10.1016\/j.envint.2024.108717_b0145","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.annepidem.2007.06.011","article-title":"Retrospective cohort mortality study of workers in a polymer production plant including a reference population of regional workers","volume":"18","author":"Leonard","year":"2008","journal-title":"Ann. Epidemiol."},{"key":"10.1016\/j.envint.2024.108717_b0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2023.139411","article-title":"Per- and polyfluoroalkyl substances and the associated thyroid cancer risk: A case-control study in China","volume":"337","author":"Li","year":"2023","journal-title":"Chemosphere"},{"key":"10.1016\/j.envint.2024.108717_b0155","doi-asserted-by":"crossref","DOI":"10.1001\/jama.2017.2719","article-title":"Trends in thyroid cancer incidence and mortality in the United States, 1974\u20132013","author":"Lim","year":"2017","journal-title":"J. Am. Med. Assoc."},{"key":"10.1016\/j.envint.2024.108717_b0160","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1007\/s40618-022-01983-4","article-title":"Endocrine-disrupting chemicals (EDCs) and cancer: new perspectives on an old relationship","volume":"46","author":"Modica","year":"2023","journal-title":"J. Endocrinol. Invest."},{"key":"10.1016\/j.envint.2024.108717_b0165","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph15081735","article-title":"Nutritional and Environmental Factors in Thyroid Carcinogenesis","volume":"15","author":"Nettore","year":"2018","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.envint.2024.108717_b0170","doi-asserted-by":"crossref","first-page":"11721","DOI":"10.1158\/0008-5472.CAN-07-2318","article-title":"SNAI1 is required for tumor growth and lymph node metastasis of human breast carcinoma MDA-MB-231 cells","volume":"67","author":"Olmeda","year":"2007","journal-title":"Cancer Res."},{"key":"10.1016\/j.envint.2024.108717_b0175","doi-asserted-by":"crossref","DOI":"10.3390\/ijms241411707","article-title":"Current review of increasing animal health threat of per- and polyfluoroalkyl substances (PFAS): harms, limitations, and alternatives to manage their toxicity","volume":"24","author":"Peritore","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.envint.2024.108717_b0180","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1210\/jc.2012-2555","article-title":"Interferon-\u03b3 and tumor necrosis factor-\u03b1 sustain secretion of specific CXC chemokines in human thyrocytes: a first step toward a differentiation between autoimmune and tumor-related inflammation?","volume":"98","author":"Rotondi","year":"2013","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"10.1016\/j.envint.2024.108717_b0185","doi-asserted-by":"crossref","first-page":"314","DOI":"10.3389\/fendo.2018.00314","article-title":"Role of chemokines in thyroid cancer microenvironment: Is CXCL8 the Main Player?","volume":"9","author":"Rotondi","year":"2018","journal-title":"Front Endocrinol (lausanne)"},{"key":"10.1016\/j.envint.2024.108717_b0190","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1111\/obr.12321","article-title":"Adiposity and risk of thyroid cancer: a systematic review and meta-analysis","volume":"16","author":"Schmid","year":"2015","journal-title":"Obes. Rev."},{"key":"10.1016\/j.envint.2024.108717_b0195","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1038\/bjc.2015.280","article-title":"Baseline and lifetime alcohol consumption and risk of differentiated thyroid carcinoma in the EPIC study","volume":"113","author":"Sen","year":"2015","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.envint.2024.108717_b0200","doi-asserted-by":"crossref","unstructured":"Sosa, J.A.; Hanna, J.W.; Robinson, K.A.; Lanman, R.B. Increases in thyroid nodule fine-needle aspirations, operations, and diagnoses of thyroid cancer in the United States. Surgery 2013;154:1420-1426; discussion 1426-1427.","DOI":"10.1016\/j.surg.2013.07.006"},{"key":"10.1016\/j.envint.2024.108717_b0205","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/nrendo.2010.87","article-title":"Environmental causes of cancer: endocrine disruptors as carcinogens","volume":"6","author":"Soto","year":"2010","journal-title":"Nat. Rev. Endocrinol."},{"key":"10.1016\/j.envint.2024.108717_b0210","doi-asserted-by":"crossref","unstructured":"Strouhalova, K.; P\u0159echov\u00e1, M.; Gandalovi\u010dov\u00e1, A.; Br\u00e1bek, J.; Gregor, M.; Rosel, D. Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers (Basel) 2020;12.","DOI":"10.3390\/cancers12010184"},{"key":"10.1016\/j.envint.2024.108717_b0215","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph17051668","article-title":"Application of the key characteristics of carcinogens to per and polyfluoroalkyl substances","volume":"17","author":"Temkin","year":"2020","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.envint.2024.108717_b0220","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1007\/s13197-022-05545-7","article-title":"Per- and polyfluoroalkyl substances (PFASs) in consumable species and food products","volume":"60","author":"Torres","year":"2023","journal-title":"J. Food Sci. Technol."},{"key":"10.1016\/j.envint.2024.108717_b0225","doi-asserted-by":"crossref","unstructured":"Usman, S.; Waseem, N.H.; Nguyen, T.K.N.; Mohsin, S.; Jamal, A.; Teh, M.T.; Waseem, A. Vimentin Is at the Heart of Epithelial Mesenchymal Transition (EMT) Mediated Metastasis. Cancers (Basel) 2021;13.","DOI":"10.3390\/cancers13194985"},{"key":"10.1016\/j.envint.2024.108717_b0230","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2023.104831","article-title":"Per- and polyfluoroalkyl substances (PFAS) exposure and thyroid cancer risk","volume":"97","author":"van Gerwen","year":"2023","journal-title":"EBioMedicine"},{"key":"10.1016\/j.envint.2024.108717_b0235","doi-asserted-by":"crossref","first-page":"2803","DOI":"10.1073\/pnas.0610733104","article-title":"Gene expression and functional evidence of epithelial-to-mesenchymal transition in papillary thyroid carcinoma invasion","volume":"104","author":"Vasko","year":"2007","journal-title":"PNAS"},{"key":"10.1016\/j.envint.2024.108717_b0240","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1289\/ehp.1205829","article-title":"Perfluorooctanoic acid exposure and cancer outcomes in a contaminated community: a geographic analysis","volume":"121","author":"Vieira","year":"2013","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.envint.2024.108717_b0245","doi-asserted-by":"crossref","DOI":"10.1155\/2015\/705169","article-title":"Tumor-associated mast cells in thyroid cancer","volume":"2015","author":"Visciano","year":"2015","journal-title":"Int. J. Endocrinol."},{"key":"10.1016\/j.envint.2024.108717_b0250","doi-asserted-by":"crossref","first-page":"24606","DOI":"10.1038\/srep24606","article-title":"Twist-mediated epithelial-mesenchymal transition promotes breast tumor cell invasion via inhibition of hippo pathway","volume":"6","author":"Wang","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.envint.2024.108717_b0255","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/S1470-2045(23)00622-8","article-title":"Carcinogenicity of perfluorooctanoic acid and perfluorooctanesulfonic acid","volume":"25","author":"Zahm","year":"2024","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.envint.2024.108717_b0260","doi-asserted-by":"crossref","first-page":"19103","DOI":"10.1038\/srep19103","article-title":"3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained","volume":"6","author":"Zanoni","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.envint.2024.108717_b0265","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2021.106745","article-title":"Varied thyroid disrupting effects of perfluorooctanoic acid (PFOA) and its novel alternatives hexafluoropropylene-oxide-dimer-acid (GenX) and ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA) in vitro","volume":"156","author":"Zhang","year":"2021","journal-title":"Environ. Int."}],"container-title":["Environment International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0160412024003039?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0160412024003039?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T03:46:12Z","timestamp":1721187972000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0160412024003039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5]]},"references-count":54,"alternative-id":["S0160412024003039"],"URL":"https:\/\/doi.org\/10.1016\/j.envint.2024.108717","relation":{},"ISSN":["0160-4120"],"issn-type":[{"value":"0160-4120","type":"print"}],"subject":[],"published":{"date-parts":[[2024,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Do PFCAs drive the establishment of thyroid cancer microenvironment? Effects of C6O4, PFOA and PFHxA exposure in two models of human thyroid cells in primary culture","name":"articletitle","label":"Article Title"},{"value":"Environment International","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.envint.2024.108717","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"108717"}}