{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T08:36:33Z","timestamp":1793349393791,"version":"4.5.0"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YC3203202-3"],"award-info":[{"award-number":["2022YC3203202-3"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010877","name":"Science, Technology and Innovation Commission of Shenzhen Municipality","doi-asserted-by":"publisher","award":["KCXFZ20211020163556020"],"award-info":[{"award-number":["KCXFZ20211020163556020"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010877","name":"Science, Technology and Innovation Commission of Shenzhen Municipality","doi-asserted-by":"publisher","award":["WDZC20200819163549002"],"award-info":[{"award-number":["WDZC20200819163549002"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Environment International"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.envint.2025.109798","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T11:25:35Z","timestamp":1758021935000},"page":"109798","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Enrichment and application of sewer-derived aerobic methanotrophs to enhance gaseous methane removal in sewers"],"prefix":"10.1016","volume":"204","author":[{"given":"Xin","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Xin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7828-4428","authenticated-orcid":false,"given":"Jiane","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.envint.2025.109798_b0005","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/s11157-018-9464-3","article-title":"Sustainable biogas mitigation and value-added resources recovery using methanotrophs intergrated into wastewater treatment plants","volume":"17","author":"AlSayed","year":"2018","journal-title":"Rev. Environ. Sci. Bio\/Technol."},{"key":"10.1016\/j.envint.2025.109798_b0010","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.biortech.2017.08.188","article-title":"Inhibitory effects of acidic pH and confounding effects of moisture content on methane biofiltration","volume":"245","author":"Barzgar","year":"2017","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.envint.2025.109798_b0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.163968","article-title":"The potential of biochar as a microbial carrier for agricultural and environmental applications","volume":"886","author":"Bolan","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envint.2025.109798_b0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2019.114914","article-title":"Different ferric dosing strategies could result in different control mechanisms of sulfide and methane production in sediments of gravity sewers","volume":"164","author":"Cao","year":"2019","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0025","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1080\/10934529.2014.910071","article-title":"A model for methane production in sewers","volume":"49","author":"Chaosakul","year":"2014","journal-title":"J. Environ. Sci. Health A"},{"key":"10.1016\/j.envint.2025.109798_b0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.155244","article-title":"Is the role of aerobic methanotrophs underestimated in methane oxidation under hypoxic conditions?","volume":"833","author":"Cheng","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envint.2025.109798_b0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.153429","article-title":"Use of methanotrophically activated biochar in novel biogeochemical cover system for carbon sequestration: Microbial characterization","volume":"821","author":"Chetri","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envint.2025.109798_b0040","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1080\/10934529.2013.815559","article-title":"Effect of trichloroethylene and tetrachloroethylene on methane oxidation and community structure of methanotrophic consortium","volume":"48","author":"Choi","year":"2013","journal-title":"J. Environ. Sci. Health A"},{"key":"10.1016\/j.envint.2025.109798_b0045","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.apsoil.2012.12.014","article-title":"Ecology of aerobic methanotrophs in controlling methane fluxes from wetlands","volume":"65","author":"Chowdhury","year":"2013","journal-title":"Appl. Soil Ecol."},{"key":"10.1016\/j.envint.2025.109798_b0050","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1099\/00221287-130-10-2565","article-title":"In vivo 13C NMR investigations of methanol oxidation by the obligate methanotroph Methylosinus trichosporium OB3b","volume":"130","author":"Cornish","year":"1984","journal-title":"Microbiology"},{"key":"10.1016\/j.envint.2025.109798_b0055","doi-asserted-by":"crossref","first-page":"5057","DOI":"10.1128\/JB.00228-08","article-title":"Formate as the main branch point for methylotrophic metabolism in Methylobacterium extorquens AM1","volume":"190","author":"Crowther","year":"2008","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.envint.2025.109798_b0060","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2022.137502","article-title":"Use of wood and cork in biofilters for the simultaneous removal of nitrates and pesticides from groundwater","volume":"313","author":"Escol\u00e0 Casas","year":"2023","journal-title":"Chemosphere"},{"key":"10.1016\/j.envint.2025.109798_b0065","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.jenvman.2014.04.023","article-title":"Impact of oxygen injection on CH4 and N2O emissions from rising main sewers","volume":"144","author":"Ganigu\u00e9","year":"2014","journal-title":"J. Environ. Manage."},{"key":"10.1016\/j.envint.2025.109798_b0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.140579","article-title":"Upstream Natural Pulsed Ventilation: a simple measure to control the sulfide and methane production in gravity sewer","volume":"742","author":"Gao","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envint.2025.109798_b0075","series-title":"Perry\u2019s Chemical Engineers\u2019 Handbook","author":"Green","year":"2007"},{"key":"10.1016\/j.envint.2025.109798_b0080","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1016\/j.watres.2007.10.014","article-title":"Methane formation in sewer systems","volume":"42","author":"Guisasola","year":"2008","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2023.131138","article-title":"Reduced sulfide and methane in rising main sewer via calcium peroxide dosing: insights from microbial physiological characteristics, metabolisms and community traits","volume":"451","author":"Guo","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.envint.2025.109798_b0090","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1111\/j.1574-6941.2012.01477.x","article-title":"Field-scale labelling and activity quantification of methane-oxidizing bacteria in a landfill-cover soil","volume":"83","author":"Henneberger","year":"2013","journal-title":"FEMS Microbiol. Ecol."},{"key":"10.1016\/j.envint.2025.109798_b0095","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1016\/j.watres.2003.11.012","article-title":"Quantification of oxygen fluxes in a long gravity sewer","volume":"38","author":"Huisman","year":"2004","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0100","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2020.116400","article-title":"Valorization of methane from environmental engineering applications: a critical review","volume":"187","author":"Jawaharraj","year":"2020","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0105","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1016\/j.watres.2012.12.036","article-title":"Effects of nitrate dosing on methanogenic activity in a sulfide-producing sewer biofilm reactor","volume":"47","author":"Jiang","year":"2013","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0110","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.apr.2019.09.018","article-title":"Methane biofiltration under different strategies of nutrient solution addition","volume":"11","author":"Khabiri","year":"2020","journal-title":"Atmos. Pollut. Res."},{"key":"10.1016\/j.envint.2025.109798_b0115","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2015.01346","article-title":"Diversity and habitat preferences of cultivated and uncultivated aerobic methanotrophic bacteria evaluated based on pmoA as molecular marker","volume":"6","author":"Knief","year":"2015","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.envint.2025.109798_b0120","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jenvman.2018.12.015","article-title":"The influence of biochar and compost mixtures, water content, and gas flow rate, on the continuous adsorption of methane in a fixed bed column","volume":"233","author":"La","year":"2019","journal-title":"J. Environ. Manage."},{"key":"10.1016\/j.envint.2025.109798_b0125","doi-asserted-by":"crossref","first-page":"753","DOI":"10.4014\/jmb.1102.01055","article-title":"Characterization of methane oxidation by a methanotroph isolated from a landfill cover soil, South Korea","volume":"21","author":"Lee","year":"2011","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.envint.2025.109798_b0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2019.133815","article-title":"Current status and future prospects of sewer biofilms: their structure, influencing factors, and substance transformations","volume":"695","author":"Li","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envint.2025.109798_b0135","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.watres.2019.06.062","article-title":"The rapid chemically induced corrosion of concrete sewers at high H2S concentration","volume":"162","author":"Li","year":"2019","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0140","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.watres.2014.09.047","article-title":"Online dissolved methane and total dissolved sulfide measurement in sewers","volume":"68","author":"Liu","year":"2015","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2023.120077","article-title":"In-situ advanced oxidation of sediment iron for sulfide control in sewers","volume":"240","author":"Liu","year":"2023","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0150","article-title":"Augmented machine learning for sewage quality assessment with limited data","volume":"23","author":"Lv","year":"2025","journal-title":"Environ. Sci. Ecotechnol."},{"key":"10.1016\/j.envint.2025.109798_b0155","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.2166\/wst.2017.103","article-title":"Liquid-gas mass transfer at drop structures","volume":"75","author":"Matias","year":"2017","journal-title":"Water Sci. Technol."},{"key":"10.1016\/j.envint.2025.109798_b0160","doi-asserted-by":"crossref","first-page":"3404","DOI":"10.1007\/s11356-018-3856-3","article-title":"Understanding the effect of ventilation, intermittent pumping and seasonality in hydrogen sulfide and methane concentrations in a coastal sewerage system","volume":"26","author":"Matos","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.envint.2025.109798_b0165","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1023\/A:1021127621257","article-title":"Microbial aerobic oxidation of methane in paddy soil","volume":"64","author":"Min","year":"2002","journal-title":"Nutr. Cycl. Agroecosyst."},{"key":"10.1016\/j.envint.2025.109798_b0170","series-title":"Monitoring and Analytical Methods of Water and Wastewater","author":"Ministry of Environmental Protection","year":"2006"},{"key":"10.1016\/j.envint.2025.109798_b0175","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1177\/0734242X09339325","article-title":"Microbial methane oxidation processes and technologies for mitigation of landfill gas emissions","volume":"27","author":"Scheutz","year":"2009","journal-title":"Waste Management & Research: the Journal for a Sustainable Circular Economy"},{"key":"10.1016\/j.envint.2025.109798_b0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.micres.2023.127534","article-title":"Techniques and mechanisms of bacteria immobilization on biochar for further environmental and agricultural applications","volume":"278","author":"Schommer","year":"2024","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.envint.2025.109798_b0185","doi-asserted-by":"crossref","first-page":"121","DOI":"10.3390\/su9010121","article-title":"Biochars as potential adsorbers of CH4, CO2 and H2S","volume":"9","author":"Sethupathi","year":"2017","journal-title":"Sustainability"},{"key":"10.1016\/j.envint.2025.109798_b0190","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.watres.2015.05.044","article-title":"Impact of fluctuations in gaseous H2S concentrations on sulfide uptake by sewer concrete: the effect of high H2S loads","volume":"81","author":"Sun","year":"2015","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0195","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.bej.2008.12.011","article-title":"Bacteria of the sulphur cycle: an overview of microbiology, biokinetics and their role in petroleum and mining industries","volume":"44","author":"Tang","year":"2009","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.envint.2025.109798_b0200","doi-asserted-by":"crossref","unstructured":"Tani, A., Mitsui, R., Nakagawa, T., 2021. Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs, in: Methods in Enzymology. Elsevier, pp. 1\u201318. Doi: 10.1016\/bs.mie.2021.01.031.","DOI":"10.1016\/bs.mie.2021.01.031"},{"key":"10.1016\/j.envint.2025.109798_b0205","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1002\/j.1551-8833.1995.tb06465.x","article-title":"Biofiltration performance: part 1, relationship to biomass","volume":"87","author":"Wang","year":"1995","journal-title":"J. AWWA"},{"key":"10.1016\/j.envint.2025.109798_b0210","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.procbio.2020.05.001","article-title":"Extracellular electron transfer of Methylophilus methylotrophs","volume":"94","author":"Yang","year":"2020","journal-title":"Process Biochem."},{"key":"10.1016\/j.envint.2025.109798_b0215","article-title":"Assessment of methane and nitrous oxide emissions from urban community sewer networks: field quantification and insights into environmental factors","volume":"28","author":"Yang","year":"2025","journal-title":"Water Res. X"},{"key":"10.1016\/j.envint.2025.109798_b0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2023.119237","article-title":"A biological strategy for sulfide control in sewers: removing sulfide by sulfur-oxidizing bacteria","volume":"348","author":"Yuan","year":"2023","journal-title":"J. Environ. Manage."},{"key":"10.1016\/j.envint.2025.109798_b0225","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/s11783-024-1879-1","article-title":"Emission of greenhouse gases from sewer networks: field assessment and isotopic characterization","volume":"18","author":"Yuan","year":"2024","journal-title":"Front. Environ. Sci. Eng."},{"key":"10.1016\/j.envint.2025.109798_b0230","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2023.120046","article-title":"Hydrogen sulfide control in sewer systems: a critical review of recent progress","volume":"240","author":"Zhang","year":"2023","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0235","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.152203","article-title":"Enhancing sulfide mitigation via the sustainable supply of oxygen from air-nanobubbles in gravity sewers","volume":"808","author":"Zhang","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envint.2025.109798_b0240","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2020.105928","article-title":"Control sulfide and methane production in sewers based on free ammonia inactivation","volume":"143","author":"Zuo","year":"2020","journal-title":"Environ. Int."},{"key":"10.1016\/j.envint.2025.109798_b0245","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2023.119820","article-title":"Reducing sulfide and methane production in gravity sewer sediments through urine separation, collection and intermittent dosing","volume":"234","author":"Zuo","year":"2023","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0250","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2024.121701","article-title":"Methane mitigation via the nitrite-DAMO process induced by nitrate dosing in sewers","volume":"257","author":"Zuo","year":"2024","journal-title":"Water Res."},{"key":"10.1016\/j.envint.2025.109798_b0255","article-title":"Nitrite-dependent microbial utilization for simultaneous removal of sulfide and methane in sewers","volume":"24","author":"Zuo","year":"2024","journal-title":"Water Res. X"}],"container-title":["Environment International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0160412025005495?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0160412025005495?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:49:09Z","timestamp":1780548549000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0160412025005495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":51,"alternative-id":["S0160412025005495"],"URL":"https:\/\/doi.org\/10.1016\/j.envint.2025.109798","relation":{},"ISSN":["0160-4120"],"issn-type":[{"value":"0160-4120","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Enrichment and application of sewer-derived aerobic methanotrophs to enhance gaseous methane removal in sewers","name":"articletitle","label":"Article Title"},{"value":"Environment International","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.envint.2025.109798","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"109798"}}