{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T09:16:53Z","timestamp":1783156613809,"version":"3.54.6"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018693","name":"Horizon Europe","doi-asserted-by":"publisher","award":["862568"],"award-info":[{"award-number":["862568"]}],"id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Environment International"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.envint.2026.110319","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T05:44:15Z","timestamp":1779428655000},"page":"110319","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Thirteen-week toxicity study in rats exposed to acetamiprid, glyphosate, tebuconazole and their mixture from prenatal life"],"prefix":"10.1016","volume":"213","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4141-9698","authenticated-orcid":false,"given":"Simona","family":"Panzacchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria","family":"Sgargi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Tibaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Truzzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana De","family":"Angelis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Falcioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Giovannini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Iuliani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Manservigi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Manzoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7393-8050","authenticated-orcid":false,"given":"Ilaria","family":"Menghetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Noferini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Strollo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul T.J.","family":"Scheepers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Mandrioli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.envint.2026.110319_b0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.etap.2024.104526","article-title":"Mechanistic insights into acetamiprid-induced genotoxicity on the myocardium and potential ameliorative role of resveratrol","volume":"110","author":"Abdelrahman","year":"2024","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"10.1016\/j.envint.2026.110319_b0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2024.177322","article-title":"Towards a comprehensive methodology for ecotoxicological assessment: Prioritizing plant protection products for mixture testing in edge-of-field surface waterbodies","volume":"956","author":"Abrantes","year":"2024","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envint.2026.110319_b0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2024.174671","article-title":"Identifying pesticides of high concern for ecosystem, plant, animal, and human health: a comprehensive field study across Europe and Argentina","volume":"948","author":"Alaoui","year":"2024","journal-title":"Sci. Total Environ."},{"issue":"10","key":"10.1016\/j.envint.2026.110319_b0020","first-page":"760","article-title":"Efficacy of essential trace elements supplementation on mineral composition, sperm characteristics, antioxidant status, and genotoxicity in testis of tebuconazole-treated rats","volume":"33","author":"Ben Saad","year":"2020","journal-title":"Biomed. Environ. Sci."},{"issue":"8","key":"10.1016\/j.envint.2026.110319_b0025","doi-asserted-by":"crossref","first-page":"23","DOI":"10.14715\/cmb\/2019.65.8.5","article-title":"Efficacy of Allium sativum oil to alleviate tebuconazol-induced oxidative stress in the liver of adult rats","volume":"65","author":"Berrouague","year":"2019","journal-title":"Cell. Mol. Biol. (Noisy-le-Grand)"},{"key":"10.1016\/j.envint.2026.110319_b0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquatox.2025.107398","article-title":"Ecotoxicological impact of the fungicide tebuconazole on fish: a historical review, global trends and challenges","volume":"284","author":"Carnib","year":"2025","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.envint.2026.110319_b0035","doi-asserted-by":"crossref","DOI":"10.3390\/toxins15010040","article-title":"Risk assessment of combined exposure to multiple chemicals at the european food safety authority: principles, guidance documents, applications and future challenges","volume":"15","author":"Cattaneo","year":"2023","journal-title":"Toxins (Basel)"},{"issue":"4","key":"10.1016\/j.envint.2026.110319_b0040","article-title":"Ergosterol distribution controls surface structure formation and fungal pathogenicity","volume":"14","author":"Choy","year":"2023","journal-title":"MBio"},{"key":"10.1016\/j.envint.2026.110319_b0045","doi-asserted-by":"crossref","unstructured":"Creasy D, Bube A, Rijk Ed, Kandori H, Kuwahara M, Masson R, Nolte T, Reams R, Regan K, Rehm S et al: Proliferative and Nonproliferative Lesions of the Rat and Mouse Male Reproductive System. Toxicologic Pathology 2012, 40(6_suppl):40S-121S.","DOI":"10.1177\/0192623312454337"},{"issue":"5","key":"10.1016\/j.envint.2026.110319_b0050","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.acthis.2016.05.009","article-title":"Effect of glyphosate on reproductive organs in male rat","volume":"118","author":"Dai","year":"2016","journal-title":"Acta Histochem."},{"key":"10.1016\/j.envint.2026.110319_b0055","doi-asserted-by":"crossref","unstructured":"EFSA, Hernandez-Jerez A, Coja T, Paparella M, Price A, Henri J, Focks A, Louisse J, Terron A, Binaglia M et al:, 2024. Statement on the toxicological properties and maximum residue levels of acetamiprid and its metabolites. EFSA J.22(5):e8759.","DOI":"10.2903\/j.efsa.2024.8759"},{"issue":"3","key":"10.1016\/j.envint.2026.110319_b0060","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1080\/10408444.2022.2091423","article-title":"Critical review and analysis of literature on low dose exposure to chemical mixtures in mammalian in vivo systems","volume":"52","author":"Elcombe","year":"2022","journal-title":"Crit. Rev. Toxicol."},{"issue":"28","key":"10.1016\/j.envint.2026.110319_b0065","doi-asserted-by":"crossref","first-page":"43114","DOI":"10.1007\/s11356-021-18331-3","article-title":"Mechanisms and histopathological impacts of acetamiprid and azoxystrobin in male rats","volume":"29","author":"El-Hak","year":"2022","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.envint.2026.110319_b0070","unstructured":"eurostat: Sales of pesticides in the EU."},{"key":"10.1016\/j.envint.2026.110319_b0075","unstructured":"FAO: FAO. 2024. Pesticides use and trade \u2013 1990\u20132022. FAOSTAT Analytical Briefs, No. 89. Rome. 2024."},{"key":"10.1016\/j.envint.2026.110319_b0080","article-title":"Disruption of the thyroid hormone system and patterns of altered thyroid hormones after gestational chemical exposures in rodents - a systematic review","volume":"14","author":"Forner-Piquer","year":"2023","journal-title":"Front Endocrinol (lausanne)"},{"issue":"3","key":"10.1016\/j.envint.2026.110319_b0085","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ejar.2020.08.007","article-title":"Pesticides pollution: Classifications, human health impact, extraction and treatment techniques","volume":"46","author":"Hassaan","year":"2020","journal-title":"Egypt. J. Aquat. Res."},{"issue":"2","key":"10.1016\/j.envint.2026.110319_b0090","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1017\/S2040174414000580","article-title":"Evolution of DOHaD: the impact of environmental health sciences","volume":"6","author":"Haugen","year":"2015","journal-title":"J. Dev. Orig. Health Dis."},{"issue":"10","key":"10.1016\/j.envint.2026.110319_b0095","doi-asserted-by":"crossref","first-page":"3416","DOI":"10.1210\/en.2015-1394","article-title":"Developmental origins of health and disease: integrating environmental influences","volume":"156","author":"Heindel","year":"2015","journal-title":"Endocrinology"},{"key":"10.1016\/j.envint.2026.110319_b0100","unstructured":"International Harmonization of Nomenclature and Diagnostic Criteria (INHAND) [https:\/\/www.toxpath.org\/inhand.asp]."},{"issue":"8","key":"10.1016\/j.envint.2026.110319_b0105","doi-asserted-by":"crossref","first-page":"4776","DOI":"10.1021\/acs.est.1c06098","article-title":"Toward assessing absolute environmental sustainability of chemical pollution","volume":"56","author":"Kosnik","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.envint.2026.110319_b0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2021.112309","article-title":"Tebuconazole induces liver injury coupled with ROS-mediated hepatic metabolism disorder","volume":"220","author":"Ku","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.envint.2026.110319_b0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2021.107038","article-title":"Glyphosate damages blood-testis barrier via NOX1-triggered oxidative stress in rats: long-term exposure as a potential risk for male reproductive health","volume":"159","author":"Liu","year":"2022","journal-title":"Environ. Int."},{"key":"10.1016\/j.envint.2026.110319_b0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2020.127792","article-title":"Gestational exposure to tebuconazole affects the development of rat fetal Leydig cells","volume":"262","author":"Ma","year":"2021","journal-title":"Chemosphere"},{"key":"10.1016\/j.envint.2026.110319_b0125","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1289\/ehp.81413","article-title":"Carcinogenicity bioassays of vinyl chloride monomer: a model of risk assessment on an experimental basis","volume":"41","author":"Maltoni","year":"1981","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.envint.2026.110319_b0130","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1289\/ehp.8982109","article-title":"Benzene, an experimental multipotential carcinogen: results of the long-term bioassays performed at the Bologna Institute of Oncology","volume":"82","author":"Maltoni","year":"1989","journal-title":"Environ. Health Perspect."},{"issue":"1","key":"10.1016\/j.envint.2026.110319_b0135","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s12940-019-0453-y","article-title":"The Ramazzini Institute 13-week pilot study glyphosate-based herbicides administered at human-equivalent dose to Sprague Dawley rats: effects on development and endocrine system","volume":"18","author":"Manservisi","year":"2019","journal-title":"Environ. Health: A Glob. Access Sci. Source"},{"key":"10.1016\/j.envint.2026.110319_b0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.jclepro.2024.143577","article-title":"Selected farm-level crop protection practices in Europe and Argentina: opportunities for moving toward sustainable use of pesticides","volume":"477","author":"Mark","year":"2024","journal-title":"J. Clean. Prod."},{"issue":"3","key":"10.1016\/j.envint.2026.110319_b0145","article-title":"Guidance on harmonised methodologies for human health, animal health and ecological risk assessment of combined exposure to multiple chemicals","volume":"17","author":"More","year":"2019","journal-title":"EFSA J."},{"key":"10.1016\/j.envint.2026.110319_b0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.167797","article-title":"Occurrence of pesticide residues in indoor dust of farmworker households across Europe and Argentina","volume":"905","author":"Navarro","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envint.2026.110319_b0155","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2024.121419","article-title":"Assessing pesticide residues occurrence and risks in water systems: a Pan-European and Argentina perspective","volume":"254","author":"Navarro","year":"2024","journal-title":"Water Res."},{"issue":"3","key":"10.1016\/j.envint.2026.110319_b0160","doi-asserted-by":"crossref","first-page":"241","DOI":"10.5487\/TR.2019.35.3.241","article-title":"Gestational exposure to pesticides induces oxidative stress and lipid peroxidation in offspring that persist at adult age in an animal model","volume":"35","author":"Ndonwi","year":"2019","journal-title":"Toxicol. Res."},{"key":"10.1016\/j.envint.2026.110319_b0165","unstructured":"Nonneoplastic Lesion Atlas - National Toxicology Program [https:\/\/ntp.niehs.nih.gov\/atlas\/nnl]."},{"key":"10.1016\/j.envint.2026.110319_b0170","unstructured":"OECD: Guidance Document 116 on the Conduct and Design of Chronic Toxicity and Carcinogenicity Studies, Supporting Test Guidelines 451, 452 and 453: Second edition, OECD Series on Testing and Assessment, No. 116, OECD Publishing, Paris,. 2014."},{"key":"10.1016\/j.envint.2026.110319_b0175","doi-asserted-by":"crossref","unstructured":"Panzacchi S, Belpoggi F, Bua L, Bucher JR, Cora MC, De Angelis L, Falcioni L, Gnudi F, Mandrioli D, Manservigi M et al:, 2024. NIEHS Report Series. In: NIEHS Report on the Toxicity Studies of Nicotine Bitartrate Dihydrate (CASRN 6019-06-3) Administered in Drinking Water to Sprague Dawley Rats and Swiss Mice: NIEHS Report 11. Research Triangle Park (NC): National Institute of Environmental Health Sciences.","DOI":"10.22427\/NIEHS-11"},{"issue":"1","key":"10.1016\/j.envint.2026.110319_b0180","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12940-025-01187-2","article-title":"Carcinogenic effects of long-term exposure from prenatal life to glyphosate and glyphosate-based herbicides in Sprague-Dawley rats","volume":"24","author":"Panzacchi","year":"2025","journal-title":"Environmental Health : a Global Access Science Source"},{"key":"10.1016\/j.envint.2026.110319_b0185","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2023.123046","article-title":"Maternal serum neonicotinoids during early-mid pregnancy and congenital heart diseases in offspring: an exploratory study","volume":"342","author":"Qu","year":"2024","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.envint.2026.110319_b0190","unstructured":"Roberts, G.K., 2023. Stout MD: Specifications for the conduct of toxicity studies by the division of translational toxicology at the National Institute of Environmental Health Sciences [Internet]."},{"key":"10.1016\/j.envint.2026.110319_b0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2023.140712","article-title":"Investigation of the miRNA levels changes to acceptable daily intake dose pesticide mixture exposure on rat mesentery and pancreas","volume":"349","author":"Sevim","year":"2024","journal-title":"Chemosphere"},{"key":"10.1016\/j.envint.2026.110319_b0200","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2025.140293","article-title":"Double trouble: the synergistic threat of environmental stressors and pesticide mixtures","volume":"500","author":"Shahid","year":"2025","journal-title":"J. Hazard. Mater."},{"issue":"11","key":"10.1016\/j.envint.2026.110319_b0205","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0259748","article-title":"Collection of human and environmental data on pesticide use in Europe and Argentina: field study protocol for the SPRINT project","volume":"16","author":"Silva","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.envint.2026.110319_b0210","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2023.108280","article-title":"Pesticide residues with hazard classifications relevant to non-target species including humans are omnipresent in the environment and farmer residences","volume":"181","author":"Silva","year":"2023","journal-title":"Environ. Int."},{"issue":"2","key":"10.1016\/j.envint.2026.110319_b0215","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1111\/j.1742-7843.2007.00200.x","article-title":"Consequences of exposure to carcinogens beginning during developmental life","volume":"102","author":"Soffritti","year":"2008","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"issue":"2","key":"10.1016\/j.envint.2026.110319_b0220","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1093\/toxsci\/kfm227","article-title":"Endocrine-disrupting activities in vivo of the fungicides tebuconazole and epoxiconazole","volume":"100","author":"Taxvig","year":"2007","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.envint.2026.110319_b0225","doi-asserted-by":"crossref","unstructured":"The Consortium For Children's Environmental H, Wirth DA, Cropper M, Axelrad DA, Bald C, Bhatnagar A, Birnbaum LS, Burke TA, Chiles TC, Geiser K et al:, 2025. Manufactured chemicals and children's health - the need for new law. N. Engl. J. Med. 392(3):299-305.","DOI":"10.1056\/NEJMms2409092"},{"issue":"11","key":"10.1016\/j.envint.2026.110319_b0230","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.3390\/nu16111556","article-title":"Implications of prenatal exposure to endocrine-disrupting chemicals in offspring development: a narrative review","volume":"16","author":"Toledano","year":"2024","journal-title":"Nutrients"},{"key":"10.1016\/j.envint.2026.110319_b0235","unstructured":"Toxicology and carcinogenesis studies of perfluorooctanoic acid administered in feed to Sprague Dawley (Hsd:Sprague Dawley SD) rats (revised). Natl Toxicol Program Tech Rep Ser 2020(598)."},{"key":"10.1016\/j.envint.2026.110319_b0240","unstructured":"US EPA Ingredients Used in Pesticide Products [https:\/\/www.epa.gov\/ingredients-used-pesticide-products\/types-pesticide-ingredients]."},{"key":"10.1016\/j.envint.2026.110319_b0245","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.149017","article-title":"Risk from pesticide mixtures - the gap between risk assessment and reality","volume":"796","author":"Weisner","year":"2021","journal-title":"Sci. Total Environ."},{"issue":"5","key":"10.1016\/j.envint.2026.110319_b0250","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1038\/cmi.2014.38","article-title":"Testicular defense systems: immune privilege and innate immunity","volume":"11","author":"Zhao","year":"2014","journal-title":"Cell. Mol. Immunol."},{"issue":"7","key":"10.1016\/j.envint.2026.110319_b0255","doi-asserted-by":"crossref","DOI":"10.3390\/toxics11070598","article-title":"Screening of toxic effects of neonicotinoid insecticides with a focus on acetamiprid: a review","volume":"11","author":"Zu\u0161\u010d\u00edkov\u00e1","year":"2023","journal-title":"Toxics"}],"container-title":["Environment International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0160412026002771?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0160412026002771?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T08:34:14Z","timestamp":1783154054000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0160412026002771"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":51,"alternative-id":["S0160412026002771"],"URL":"https:\/\/doi.org\/10.1016\/j.envint.2026.110319","relation":{},"ISSN":["0160-4120"],"issn-type":[{"value":"0160-4120","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Thirteen-week toxicity study in rats exposed to acetamiprid, glyphosate, tebuconazole and their mixture from prenatal life","name":"articletitle","label":"Article Title"},{"value":"Environment International","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.envint.2026.110319","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"110319"}}