{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T06:48:22Z","timestamp":1791787702470,"version":"4.3.4"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017343","name":"Center for High Performance Computing","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017343","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Environmental Pollution"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1016\/j.envpol.2026.127943","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T07:04:25Z","timestamp":1773126265000},"page":"127943","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Longitudinal wastewater metagenomics reveals distinct environmental and anthropogenic associations with resistance, virulence, and viral communities"],"prefix":"10.1016","volume":"397","author":[{"given":"J\u00falia Firme","family":"Freitas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais Teixeira","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0642-3162","authenticated-orcid":false,"given":"Lucymara Fassarella","family":"Agnez-Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.envpol.2026.127943_bib1","doi-asserted-by":"crossref","DOI":"10.1186\/s12864-024-10876-0","article-title":"Metagenomic assemblies tend to break around antibiotic resistance genes","author":"Abramova","year":"2024","journal-title":"BMC Genom."},{"key":"10.1016\/j.envpol.2026.127943_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AAC.00780-17","article-title":"The tet39 determinant and the msrE-mphE genes in Acinetobacter plasmids are each part of discrete modules flanked by inversely oriented pdif (XerC-XerD) sites","volume":"61","author":"Blackwell","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.envpol.2026.127943_bib3","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.jes.2023.04.012","article-title":"A global perspective on microbial risk factors in effluents of wastewater treatment plants","volume":"138","author":"Chen","year":"2024","journal-title":"J. Environ. Sci. (China)."},{"key":"10.1016\/j.envpol.2026.127943_bib4","article-title":"Modeling the limits of detection for antimicrobial resistance genes in agri - food samples : a comparative analysis of bioinformatics tools","volume":"1\u201320","author":"Cooper","year":"2024","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.envpol.2026.127943_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.165984","article-title":"Removal of intI1, ARGs, and SARS-CoV-2 and changes in bacterial communities in four sewage treatment facilities","volume":"903","author":"Cuetero-Mart\u00ednez","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envpol.2026.127943_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.mib.2024.102471","article-title":"Virulence-associated factors as targets for phage infection","volume":"79","author":"de Melo","year":"2024","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.envpol.2026.127943_bib7","doi-asserted-by":"crossref","DOI":"10.1186\/s40168-020-00993-9","article-title":"PathoFact: a pipeline for the prediction of virulence factors and antimicrobial resistance genes in metagenomic data","volume":"9","author":"de Nies","year":"2021","journal-title":"Microbiome"},{"key":"10.1016\/j.envpol.2026.127943_bib8","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1038\/s41396-019-0478-9","article-title":"Viruses as key reservoirs of antibiotic resistance genes in the environment","volume":"13","author":"Debroas","year":"2019","journal-title":"ISME J."},{"key":"10.1016\/j.envpol.2026.127943_bib9","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/s41576-023-00649-y","article-title":"Genomic surveillance for antimicrobial resistance \u2014 a one Health perspective","volume":"25","author":"Djordjevic","year":"2024","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.envpol.2026.127943_bib41","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1038\/s41564-019-0494-6","article-title":"Global Phylogeography and Ancient Evolution of the Widespread Human Gut Virus Crassphage","volume":"4","author":"Edwards","year":"2019","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.envpol.2026.127943_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/msystems.00651-22","article-title":"A metagenomic investigation of spatial and temporal changes in sewage microbiomes across a university campus","volume":"7","author":"Fierer","year":"2022","journal-title":"mSystems"},{"key":"10.1016\/j.envpol.2026.127943_bib11","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1007\/s100960050398","article-title":"Class 1 integrons, gene cassettes, mobility, and epidemiology","volume":"18","author":"Fluit","year":"1999","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.envpol.2026.127943_bib12","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkad384","article-title":"ResFinderFG v2.0: a database of antibiotic resistance genes obtained by functional metagenomics","volume":"51","author":"Gschwind","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.envpol.2026.127943_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2017.07.002","article-title":"Metagenomic analysis reveals wastewater treatment plants as hotspots of antibiotic resistance genes and mobile genetic elements Jianhua","author":"Guo","year":"2017","journal-title":"Water Res."},{"key":"10.1016\/j.envpol.2026.127943_bib14","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-019-08853-3","article-title":"Global monitoring of antimicrobial resistance based on metagenomics analyses of urban sewage","volume":"10","author":"Hendriksen","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.envpol.2026.127943_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2012.00002","article-title":"IncP-1\u03b5 plasmids are important vectors of antibiotic resistance genes in agricultural systems: diversification driven by class 1 integron gene cassettes","volume":"3","author":"Heuer","year":"2012","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.envpol.2026.127943_bib16","first-page":"1","article-title":"Realising a global One Health disease surveillance approach: insights from wastewater and beyond","volume":"15","author":"Hill","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.envpol.2026.127943_bib17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mSphere.00452-21","article-title":"Distribution of antimicrobial resistance and virulence genes within the prophage-associated regions in nosocomial pathogens","volume":"6","author":"Kondo","year":"2021","journal-title":"mSphere"},{"key":"10.1016\/j.envpol.2026.127943_bib18","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/s41579-021-00649-x","article-title":"Antibiotic resistance in the environment","volume":"20","author":"Larsson","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.envpol.2026.127943_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2021.125865","article-title":"Evaluating the effect of gradient applied voltages on antibiotic resistance genes proliferation and biogas production in anaerobic electrochemical membrane bioreactor","volume":"416","author":"Li","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.envpol.2026.127943_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2022.107381","article-title":"Anaerobic electrochemical membrane bioreactor effectively mitigates antibiotic resistance genes proliferation under high antibiotic selection pressure","volume":"166","author":"Li","year":"2022","journal-title":"Environ. Int."},{"key":"10.1016\/j.envpol.2026.127943_bib21","doi-asserted-by":"crossref","first-page":"8315","DOI":"10.1038\/s41467-024-52450-y","article-title":"Prophage-encoded antibiotic resistance genes are enriched in human-impacted environments","volume":"15","author":"Liao","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.envpol.2026.127943_bib22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13756-024-01412-6","article-title":"Consumption of antibiotics in Brazil - an analysis of sales data between 2014 and 2019","volume":"13","author":"Lopes","year":"2024","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"10.1016\/j.envpol.2026.127943_bib23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2023.1037845","article-title":"Metagenome-assembled genomes indicate that antimicrobial resistance genes are highly prevalent among urban bacteria and multidrug and glycopeptide resistances are ubiquitous in most taxa","volume":"14","author":"Magn\u00fasd\u00f3ttir","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.envpol.2026.127943_bib24","article-title":"Variations in bacterial communities and antibiotic resistance genes across diverse recycled and surface water irrigation sources in the mid-atlantic and Southwest United States","author":"Malayil","year":"2022","journal-title":"A CONSERVE Two-Year Field Study"},{"key":"10.1016\/j.envpol.2026.127943_bib25","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1021\/acsestwater.4c01278","article-title":"Characterization of aquatic Acinetobacter baumannii during the COVID-19 outbreak in Wuhan, China","volume":"5","author":"Mao","year":"2025","journal-title":"ACS ES&T Water"},{"key":"10.1016\/j.envpol.2026.127943_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-022-34312-7","article-title":"Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance","volume":"13","author":"Munk","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.envpol.2026.127943_bib27","doi-asserted-by":"crossref","DOI":"10.1021\/acs.est.2c01570","article-title":"Antibiotic resistomes and microbiomes in the surface water along the code River in Indonesia reflect drainage Basin anthropogenic activities","volume":"56","author":"Muurinen","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.envpol.2026.127943_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.146964","article-title":"Science of the Total Environment monitoring antibiotic resistance genes in wastewater treatment : current strategies and future challenges","volume":"783","author":"Nguyen","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envpol.2026.127943_bib29","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-024-10256-9","article-title":"Arcobacter species isolated from human stool samples, animal products, ready-to-eat salad mixes, and ambient water: prevalence, antimicrobial susceptibility, and virulence gene profiles","volume":"24","author":"Niyayesh","year":"2024","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.envpol.2026.127943_bib30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/0037-8682-0861-2020","article-title":"Antibiotic use in brazilian hospitals in the 21st century: a systematic review","volume":"54","author":"Pereira","year":"2021","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"10.1016\/j.envpol.2026.127943_bib31","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.jhin.2019.10.016","article-title":"Global point prevalence survey of antimicrobial consumption in Brazilian hospitals","volume":"104","author":"Porto","year":"2020","journal-title":"J. Hosp. Infect."},{"key":"10.1016\/j.envpol.2026.127943_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.onehlt.2025.101139","article-title":"The surveillance of antimicrobial resistance in wastewater from a one health perspective : a global scoping and temporal review (2014 \u2013 2024)","volume":"21","author":"Punch","year":"2025","journal-title":"One Health"},{"key":"10.1016\/j.envpol.2026.127943_bib33","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1080\/01652176.2017.1323355","article-title":"Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control - a comprehensive review","volume":"37","author":"Ramees","year":"2017","journal-title":"Vet. Q."},{"key":"10.1016\/j.envpol.2026.127943_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2025.180607","article-title":"Science of the Total Environment Genome-resolved metagenomics uncovers antimicrobial resistance gene carriers in hospital and municipal wastewater environments","volume":"1002","author":"Silvester","year":"2025","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envpol.2026.127943_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2023.121325","article-title":"Extracellular DNA includes an important fraction of high-risk antibiotic resistance genes in treated wastewaters","volume":"323","author":"Sivalingam","year":"2023","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.envpol.2026.127943_bib36","series-title":"Opportunities and Challenges of Multiomics for Discovery and Monitoring of Human Pathogens","author":"Turner","year":"2025"},{"key":"10.1016\/j.envpol.2026.127943_bib37","doi-asserted-by":"crossref","DOI":"10.1093\/bioinformatics\/btaf173","article-title":"argNorm: normalization of antibiotic resistance gene annotations to the Antibiotic Resistance Ontology (ARO)","volume":"41","author":"Ugarcina Perovic","year":"2025","journal-title":"Bioinformatics"},{"key":"10.1016\/j.envpol.2026.127943_bib38","doi-asserted-by":"crossref","first-page":"7928","DOI":"10.1016\/j.biortech.2008.02.044","article-title":"Optimisation of the anaerobic digestion of agricultural resources","volume":"99","author":"Ward","year":"2008","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.envpol.2026.127943_bib39","author":"Zhang"},{"key":"10.1016\/j.envpol.2026.127943_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.165441","article-title":"Prevalence of antibiotic resistance genes and virulence factors in the sediment of WWTP effluent-dominated rivers","volume":"897","author":"Zou","year":"2023","journal-title":"Sci. Total Environ."}],"container-title":["Environmental Pollution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0269749126003131?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0269749126003131?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T06:30:19Z","timestamp":1791786619000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0269749126003131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":41,"alternative-id":["S0269749126003131"],"URL":"https:\/\/doi.org\/10.1016\/j.envpol.2026.127943","relation":{},"ISSN":["0269-7491"],"issn-type":[{"value":"0269-7491","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Longitudinal wastewater metagenomics reveals distinct environmental and anthropogenic associations with resistance, virulence, and viral communities","name":"articletitle","label":"Article Title"},{"value":"Environmental Pollution","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.envpol.2026.127943","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"127943"}}