{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T21:36:30Z","timestamp":1776720990441,"version":"3.51.2"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T00:00:00Z","timestamp":1690934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000201","name":"U.S. Department of the Interior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000201","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007516","name":"National Park Service","doi-asserted-by":"publisher","award":["P20PG00222"],"award-info":[{"award-number":["P20PG00222"]}],"id":[{"id":"10.13039\/100007516","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Environmental Research"],"published-print":{"date-parts":[[2023,11]]},"DOI":"10.1016\/j.envres.2023.116817","type":"journal-article","created":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T11:08:17Z","timestamp":1690974497000},"page":"116817","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"P2","title":["Employing molecular, chemical and physiological techniques using Crassostrea virginica to assess ecosystem health along coastal South Carolina and North Carolina, United States"],"prefix":"10.1016","volume":"236","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3796-4896","authenticated-orcid":false,"given":"Allisan","family":"Aquilina-Beck","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lou Ann","family":"Reed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Rider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Burdine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Daugomah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis","family":"Apeti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pete","family":"Key","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"DeLorenzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.envres.2023.116817_bib1","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.ecoenv.2019.02.009","article-title":"Toxicity of as in Crassostrea virginica (Gmelin, 1791) from the Northern Gulf of Mexico at the presence of Zn and its antioxidant defense mechanisms","volume":"172","author":"Alexander","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.envres.2023.116817_bib2","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1002\/etc.34","article-title":"Adverse Outcome pathways: a conceptual framework to support ecotoxicology research and risk assessment","volume":"29","author":"Ankley","year":"2010","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.envres.2023.116817_bib3","first-page":"39","article-title":"National status and trends mussel Watch program","volume":"134","author":"Apeti","year":"2012","journal-title":"Sampl. Methods 2012 Update"},{"key":"10.1016\/j.envres.2023.116817_bib4","first-page":"120","article-title":"An assessment of contaminants of emerging concern in chesapeake bay, MD and Charleston harbor","volume":"240","author":"Apeti","year":"2018","journal-title":"SC"},{"key":"10.1016\/j.envres.2023.116817_bib5","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s00244-020-00765-4","article-title":"Uptake and biological effects of Perfluorooctane Sulfonate exposure in the adult eastern oyster Crassostrea virginica","volume":"79","author":"Aquilina-Beck","year":"2020","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"10.1016\/j.envres.2023.116817_bib6","first-page":"1","article-title":"Histopathological indices and inflammatory response in the digestive gland of the mussel Mytilus galloprovincialis as biomarker of immunotoxicity to silver nanoparticles","author":"Bouallegui","year":"2017","journal-title":"Biomarkers"},{"key":"10.1016\/j.envres.2023.116817_bib7","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.ecoenv.2006.02.001","article-title":"Effects of the insecticide permethrin on three life stages of the grass shrimp, Palaemonetes pugio","volume":"64","author":"DeLorenzo","year":"2006","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.envres.2023.116817_bib8","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/sum.12221","article-title":"Regional trends in Scottish advisory soil acidity and phosphorus results: significance of management history, land use and soil attributes","volume":"32","author":"Edwards","year":"2016","journal-title":"Soil Use Manag."},{"key":"10.1016\/j.envres.2023.116817_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.139886","article-title":"Oxidative stress ecology on Pacific oyster Crassostrea gigas from lagoon and offshore Italian sites","volume":"739","author":"Elia","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envres.2023.116817_bib10","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1080\/1354750X.2021.1878558","article-title":"Toxicity of gamma aluminium oxide nanoparticles in the Mediterranean mussel (Mytilus galloprovincialis): histopathological alterations and antioxidant responses in the gill and digestive gland","volume":"26","author":"Erturk Gurkan","year":"2021","journal-title":"Biomarkers"},{"key":"10.1016\/j.envres.2023.116817_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.marpolbul.2016.05.074","article-title":"Edward D. Goldberg's proposal of \u201cthe mussel Watch\u201d: reflections after 40 years","volume":"110","author":"Farrington","year":"2016","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.envres.2023.116817_bib12","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.aquatox.2008.03.009","article-title":"Expression of metallothionein mRNAs by in situ hybridization in the gills of Mytilus galloprovincialis, from natural polluted environments","volume":"88","author":"Fasulo","year":"2008","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.envres.2023.116817_bib13","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.ecoenv.2005.01.004","article-title":"Do seasonal changes affect metallothionein induction by metals in mussels, Mytilus edulis?","volume":"61","author":"Geffard","year":"2005","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.envres.2023.116817_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.146731","article-title":"Effects of nonylphenol exposure on histological changes, apoptosis and time-course transcriptome in gills of white shrimp Litopenaeus vannamei","volume":"781","author":"Guo","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envres.2023.116817_bib15","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3390\/toxins13010021","article-title":"Tissue-biased and species-specific regulation of glutathione peroxidase (GPx) genes in scallops exposed to toxic Dinoflagellates","volume":"13","author":"Hlaing","year":"2020","journal-title":"Toxins"},{"key":"10.1016\/j.envres.2023.116817_bib16","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0022-0981(03)00357-5","article-title":"Linkages between tidal creek ecosystems and the landscape and demographic attributes of their watersheds","volume":"298","author":"Holland","year":"2004","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"10.1016\/j.envres.2023.116817_bib17","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.gene.2006.05.004","article-title":"Regulation of metallothionein genes in the American oyster (Crassostrea virginica): ontogeny and differential expression in response to different stressors","volume":"379","author":"Jenny","year":"2006","journal-title":"Gene"},{"key":"10.1016\/j.envres.2023.116817_bib18","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1002\/etc.4367","article-title":"A 72-h exposure study with eastern oysters (Crassostrea virginica) and the nanomaterial graphene oxide","volume":"38","author":"Khan","year":"2019","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.envres.2023.116817_bib19","first-page":"105","article-title":"An assessment of two Decades of contaminant monitoring in the nation's coastal zone","volume":"74","author":"Kimbrough","year":"2008","journal-title":"NOAA Tech. Memo. NOS NGS"},{"key":"10.1016\/j.envres.2023.116817_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.142921","article-title":"Comparison of imidacloprid, propiconazole, and nanopropiconazole effects on the development, behavior, and gene expression biomarkers of the Pacific oyster (Magallana gigas)","volume":"764","author":"Kuchovska","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envres.2023.116817_bib21","doi-asserted-by":"crossref","first-page":"403","DOI":"10.2147\/CMAR.S154608","article-title":"Bax, Bcl-2, and Bax\/Bcl-2 as prognostic markers in acute myeloid leukemia: are we ready for Bcl-2-directed therapy?","volume":"10","author":"Kulsoom","year":"2018","journal-title":"Cancer Manag. Res."},{"key":"10.1016\/j.envres.2023.116817_bib22","series-title":"Quality NNSaTPfME, Editor","article-title":"Contaminant trends in US national estuarine research reserves","author":"Lauenstein","year":"2002"},{"key":"10.1016\/j.envres.2023.116817_bib23","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0048-9697(01)00897-X","article-title":"The status and trends of trace element and organic contaminants in oysters, Crassostrea virginica, in the waters of the Carolinas, USA","volume":"285","author":"Lauenstein","year":"2002","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envres.2023.116817_bib24","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.aquatox.2013.01.022","article-title":"Inter-site differences of zinc susceptibility of th eoyster Crassostrea hongkongensis","author":"Liu","year":"2013","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.envres.2023.116817_bib25","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.envres.2023.116817_bib26","article-title":"Hydrocarbons occurrence and transcriptomic response of oyster Crassostrea virginica from Lagoons of the Southern Gulf of Mexico","volume":"19","author":"Lopez-Landavery","year":"2023","journal-title":"Front. Mar. Sci."},{"key":"10.1016\/j.envres.2023.116817_bib27","doi-asserted-by":"crossref","first-page":"2602","DOI":"10.1002\/etc.3797","article-title":"Differential effects of zinc exposure on male and female oysters (Crassostrea angulata) as revealed by label-free quantitative proteomics","volume":"36","author":"Luo","year":"2017","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.envres.2023.116817_bib28","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.aquatox.2013.04.015","article-title":"Tissue specific responses of oysters, Crassostrea virginica, to silver nanoparticles","volume":"138\u2013139","author":"McCarthy","year":"2013","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.envres.2023.116817_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.marpolbul.2012.06.017","article-title":"Effect of zinc sacrificial anode degradation on the defence system o fhte Pacific oyster, Crassostrea gigas: chronic and acute exposures","volume":"64","author":"Mottin","year":"2012","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.envres.2023.116817_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.envres.2023.116817_bib31","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.scitotenv.2015.06.022","article-title":"Investigating physiological, cellular and molecular effects in juvenile blue crab, Callinectus sapidus, exposed to field-collected sediments contaminated by oil from the Deepwater Horizon Inciden","volume":"532","author":"Pie","year":"2015","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.envres.2023.116817_bib32","first-page":"322","article-title":"Progress in ecotoxicogenomics for environmental monitoring, mode of action, and toxicant identification","volume":"2","author":"Poynton","year":"2008","journal-title":"Adv. Exp. Biol."},{"key":"10.1016\/j.envres.2023.116817_bib33","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.jtemb.2014.07.019","article-title":"Zinc: an antioxidant and anti-inflammatory agent: role of zinc in degenerative disorders of aging","volume":"28","author":"Prasad","year":"2014","journal-title":"J. Trace Elem. Med. Biol."},{"key":"10.1016\/j.envres.2023.116817_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2020.110428","article-title":"Effects of elevated temperature on prooxidant-antioxidant homeostasis and redox status in the American oyster: signaling pathways of cellular apoptosis during heat stress","volume":"196","author":"Rahman","year":"2021","journal-title":"Environ. Res."},{"key":"10.1016\/j.envres.2023.116817_bib35","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.fct.2013.09.026","article-title":"An update on oxidative stress-mediated organ pathophysiology","volume":"62","author":"Rashid","year":"2013","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.envres.2023.116817_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/0166-445X(94)00064-W","article-title":"Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: implications for the use of biochemical biomarkers","volume":"31","author":"Regoli","year":"1995","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.envres.2023.116817_bib37","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1023\/A:1003094515903","article-title":"Defenses againstoxidative stress in the Antarctic scallop Adamussium colbecki and effects of acute exposure to metals","volume":"355","author":"Regoli","year":"1997","journal-title":"Hygrobiology"},{"key":"10.1016\/j.envres.2023.116817_bib38","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/S0141-1136(02)00181-2","article-title":"Seasonal variation in lysosomal destabilization in oysters, Crassostrea virginica","volume":"54","author":"Ringwood","year":"2002","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.envres.2023.116817_bib39","series-title":"Cellular Biomarkers (Lysosomal Destabilization, Glutathione and Lipid Peroxidation) in Three Common Estuarine Species: A Methods Handbook","first-page":"49","author":"Ringwood","year":"2003"},{"key":"10.1016\/j.envres.2023.116817_bib40","doi-asserted-by":"crossref","first-page":"7136","DOI":"10.1021\/es900621j","article-title":"Fullerene exposures with oysters: embryonic, adult, and cellular responses","volume":"43","author":"Ringwood","year":"2009","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.envres.2023.116817_bib41","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/s002449900539","article-title":"Tidal creek and salt marsh sediments in South Carolina coastal estuaries: I. Distribution of trace metals","volume":"37","author":"Sanger","year":"1999","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"10.1016\/j.envres.2023.116817_bib42","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1007\/s002449900540","article-title":"Tidal creek and salt marsh sediments in South Carolina coastal estuaries: II. Distribution of organic contaminants","volume":"37","author":"Sanger","year":"1999","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"10.1016\/j.envres.2023.116817_bib43","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1515\/BC.2007.126","article-title":"Molecular biology of glutathione peroxidase 4: from genomic structure to developmental expression and neural function","volume":"388","author":"Savaskan","year":"2007","journal-title":"Biol. Chem."},{"key":"10.1016\/j.envres.2023.116817_bib44","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.ecoenv.2017.03.056","article-title":"Antioxidant responses in gills and digestive gland of oyster Crassostrea madrasensis (Preston) under lead exposure","volume":"142","author":"Shenai-Tirodkar","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.envres.2023.116817_bib45","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.aquatox.2011.09.018","article-title":"Proteomic discovery of biomarkers of metal contamination in Sydney Rock oyster (Saccostrea glomerata)","volume":"109","author":"Thompson","year":"2012","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.envres.2023.116817_bib46","series-title":"Method 3005A (SW-846): Acid Digestion of Waters for Total Recoverable or Dissolved Metals for Analysis by Flame AA or ICP Spectroscopy","year":"1992"},{"key":"10.1016\/j.envres.2023.116817_bib47","series-title":"Method 200.8 (SAM): Determination of Trace Elements in Waters and Wastes by Inductively Coupled Plasma-Mass Spectrometry","year":"1994"},{"key":"10.1016\/j.envres.2023.116817_bib48","series-title":"Method 3050B (SW-846): Acid Digestion of Sediments, Sludges, and Soils","year":"1996"},{"key":"10.1016\/j.envres.2023.116817_bib49","series-title":"Method 6010D (SAM): Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES)","year":"2014"},{"key":"10.1016\/j.envres.2023.116817_bib50","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1039\/C8EM00069G","article-title":"Trace metals in oysters: molecular and cellular mechanisms and ecotoxicological impacts","volume":"20","author":"Wang","year":"2018","journal-title":"Environ. Sci. Process. Impacts"},{"key":"10.1016\/j.envres.2023.116817_bib51","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1093\/jn\/134.3.489","article-title":"Glutathione metabolism and its implications for health","volume":"134","author":"Wu","year":"2004","journal-title":"J. Nutr."},{"key":"10.1016\/j.envres.2023.116817_bib52","doi-asserted-by":"crossref","DOI":"10.3390\/cells9071679","article-title":"The ins and outs of cathepsins: physiological function and role in Disease management","volume":"9","author":"Yadati","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.envres.2023.116817_bib53","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.marenvres.2017.03.005","article-title":"Acclimatory processes are likely responsible for metal tolerance in oyster embryos","volume":"127","author":"Yingprasertchai","year":"2017","journal-title":"Mar. Environ. Res."}],"container-title":["Environmental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0013935123016213?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0013935123016213?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T20:37:28Z","timestamp":1776717448000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0013935123016213"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11]]},"references-count":53,"alternative-id":["S0013935123016213"],"URL":"https:\/\/doi.org\/10.1016\/j.envres.2023.116817","relation":{},"ISSN":["0013-9351"],"issn-type":[{"value":"0013-9351","type":"print"}],"subject":[],"published":{"date-parts":[[2023,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Employing molecular, chemical and physiological techniques using Crassostrea virginica to assess ecosystem health along coastal South Carolina and North Carolina, United States","name":"articletitle","label":"Article Title"},{"value":"Environmental Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.envres.2023.116817","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"116817"}}