{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T21:24:07Z","timestamp":1776979447267,"version":"3.51.4"},"reference-count":44,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de Ensino Superior (CAPES)"},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00ed\ufb01co e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme and Microbial Technology"],"published-print":{"date-parts":[[2013,3]]},"DOI":"10.1016\/j.enzmictec.2012.12.007","type":"journal-article","created":{"date-parts":[[2012,12,31]],"date-time":"2012-12-31T12:31:00Z","timestamp":1356957060000},"page":"141-150","source":"Crossref","is-referenced-by-count":79,"title":["Protic ionic liquid as additive on lipase immobilization using silica sol\u2013gel"],"prefix":"10.1016","volume":"52","author":[{"given":"Ranyere Lucena","family":"de Souza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuelle Lima Pache","family":"de Faria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renan Tavares","family":"Figueiredo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisiane dos Santos","family":"Freitas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Iglesias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Mattedi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisella Maria","family":"Zanin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"On\u00e9lia Aparecida Andreo","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o A.P.","family":"Coutinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1lvaro Silva","family":"Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cleide Mara Faria","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.enzmictec.2012.12.007_bib0005","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0960-8524(01)00203-6","article-title":"Immobilized Candida antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium","volume":"83","author":"Kose","year":"2002","journal-title":"Bioresource Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0010","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.molcatb.2006.01.005","article-title":"Characterization of sol\u2013gel encapsulated lipase using tetraethoxysilane as precursor","volume":"39","author":"Soares","year":"2006","journal-title":"Journal of Molecular Catalysis B: Enzymatic"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0015","doi-asserted-by":"crossref","first-page":"2800","DOI":"10.1016\/j.biortech.2007.06.020","article-title":"Studies of optimum conditions for covalent immobilization of Candida rugosa lipase on poly(gamma-glutamic acid) by RSM","volume":"99","author":"Chang","year":"2008","journal-title":"Bioresource Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0020","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1007\/s12010-010-8907-2","article-title":"Partitioning of porcine pancreatic lipase in a two-phase systems of polyethylene glycol\/potassium phosphate aqueous","volume":"161","author":"Souza","year":"2010","journal-title":"Applied Biochemistry and Biotechnology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0025","first-page":"3853","article-title":"Purification of lipase produced by a new source of Bacillus in submerged fermentation using an aqueous two-phase system","volume":"879","author":"Barbosa","year":"2011","journal-title":"Journal of Molecular Catalysis B: Enzymatic"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0030","series-title":"Enzimas em biotecnologia:produ\u00e7\u00e3o, aplica\u00e7\u00e3o e mercado. ed.","author":"Bon","year":"2008"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0035","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1007\/s10529-009-0076-4","article-title":"Advances in enzyme immobilisation","volume":"3","author":"Brady","year":"2009","journal-title":"Biotechnology Letters"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0040","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.enzmictec.2011.06.023","article-title":"Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization","volume":"49","author":"Cowan","year":"2011","journal-title":"Enzyme and Microbial Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0045","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.biortech.2005.02.029","volume":"97","author":"Yang","year":"2006","journal-title":"Bioresource Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0050","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.biortech.2004.05.029","article-title":"Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil","volume":"96","author":"Noureddini","year":"2005","journal-title":"Bioresource Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0055","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.enzmictec.2010.10.003","article-title":"Control of protein immobilization:coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance","volume":"48","author":"Hernandez","year":"2011","journal-title":"Enzyme and Microbial Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0060","doi-asserted-by":"crossref","first-page":"5459","DOI":"10.1016\/j.biortech.2007.11.009","article-title":"Covalent immobilization of lipase from Candida rugosaonto poly(acrylonitrile-co-2-hydroxyethyl methacrylate) eletrospun fibrous membranes for potential bioreactor application","volume":"99","author":"Huang","year":"2008","journal-title":"Bioresource Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0065","first-page":"1397","article-title":"Efficient immobilization of lipase by entrapment in hydrophobic sol\u2013gel materials","volume":"49","author":"Reetz","year":"1996","journal-title":"Journal of Sol\u2013Gel Science and Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0070","series-title":"Immobilization of enzymes and cells","author":"Guisan","year":"2006"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0075","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.molcatb.2010.07.018","article-title":"Supported ionic liquids in Burkholderia cepacia lipase-catalyzed asymmetric acylation","volume":"67","author":"Hara","year":"2010","journal-title":"Journal of Molecular Catalysis B: Enzymatic"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0080","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.molcatb.2007.05.002","article-title":"In\ufb02uence of ionic liquids as additives on sol\u2013gel immobilized lipase","volume":"47","author":"Lee","year":"2007","journal-title":"Journal of Molecular Catalysis B: Enzymatic"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0085","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.molcatb.2010.01.027","article-title":"Preparation and properties of xerogels obtained by ionic liquid incorporation during the immobilization of lipase by the sol\u2013gel method","volume":"65","author":"Zarcula","year":"2010","journal-title":"Journal of Molecular Catalysis B: Enzymatic"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0225","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1016\/j.enzmictec.2007.01.018","article-title":"Improvement of enzyme activity, stability andselectivity via immobilization techniques","volume":"40","author":"Mateo","year":"2007","journal-title":"Enzyme and Microbial Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0095","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.fluid.2010.08.022","article-title":"Synthesis and thermophysical properties of two new protic long-chain ionic liquids with the oleate anion","volume":"299","author":"\u00c1lvarez","year":"2010","journal-title":"Fluid Phase Equilibria"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0100","first-page":"625","article-title":"Br\u00f8nsted ionic liquids for sustainable processes:synthesis and physical properties","volume":"55","author":"\u00c1lvarez","year":"2010","journal-title":"Journal of Chemical Engineering"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0105","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1039\/c2gc35196j","article-title":"Effective resolution of 1-phenyl ethanol by Candida antarctica lipase B catalysed acylation with vinyl acetate in protic ionic liquids (PILs)","volume":"14","author":"R\u00edos","year":"2012","journal-title":"Green Chemistry"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0110","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1021\/cr068040u","article-title":"Protic ionic liquids:properties and applications","volume":"108","author":"Greaves","year":"2008","journal-title":"Chemical Reviews"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0115","unstructured":"INPI Patent submission No. PI0306829-3, 11 September 2003."},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0120","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1385\/ABAB:113:1-3:307","article-title":"Influence of the alkyl-substituted silane precursor on sol\u2013gel encapsulated lipase activity","volume":"113","author":"Soares","year":"2004","journal-title":"Applied Biochemistry and Biotechnology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0125","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1021\/ja01269a023","article-title":"Adsorption of gases in multimolecular layers","volume":"60","author":"Brunauer","year":"1938","journal-title":"Journal of the American Chemical Society"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0130","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.bej.2009.10.002","article-title":"Recent advances of enzymatic reactions in ionic liquids","volume":"48","author":"Moniruzzaman","year":"2010","journal-title":"Biochemical Engineering Journal"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0135","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1002\/jctb.2375","article-title":"Methods for stabilizing and activating enzymes in ionic liquids \u2013 a review","volume":"85","author":"Zhao","year":"2010","journal-title":"Journal of Chemical Technology and Biotechnology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0140","doi-asserted-by":"crossref","unstructured":"Rodrigues RC, Ortiz C, Berenguer-Murcia A, Torres R, Fern\u00e1ndez-Lafuente R. \u03b1-Modifying enzyme activity and selectivity by immobilization. Chemical Society Reviews 2013;on line.","DOI":"10.1002\/chin.201339264"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0145","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jbiotec.2010.08.005","article-title":"\u03b1-Rhamnosidase and \u03b2-glucosidase expressed by naringinase immobilized on new ionic liquid sol\u2013gel matrices:activity and stability studies","volume":"152","author":"Vila-Real","year":"2011","journal-title":"Journal of Biotechnology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0150","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1080\/10242420701379213","article-title":"The effect of ionic liquid media on activity, selectivity and stability of Candida antarctica lipase B in transesterification reactions","volume":"25","author":"R\u00edos","year":"2007","journal-title":"Biocatalysis and Biotransformation"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0155","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1002\/adsc.200700303","article-title":"Supported ionic liquid enzymatic catalysis for the production of biodiesel","volume":"350","author":"Gamba","year":"2008","journal-title":"Advanced Synthesis and Catalysis"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0160","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.enzmictec.2007.03.017","article-title":"Lipase-catalyzed biodiesel production from soybean oil in ionic liquids","volume":"41","author":"Ha","year":"2007","journal-title":"Enzyme and Microbial Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0165","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.enzmictec.2009.08.009","article-title":"Stabilization of multimeric enzymes:strategies to prevent subunit dissociation","volume":"45","author":"Fernandez-Lafuente","year":"2009","journal-title":"Enzyme and Microbial Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0170","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1016\/j.procbio.2008.05.016","article-title":"Reversible immobilization of a hexameric \u03b1-galactosidase from Thermus sp. strain T2 on polymeric ionic exchangers","volume":"43","author":"Filho","year":"2008","journal-title":"Process Biochemistry"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0175","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0141-0229(02)00307-1","article-title":"Reversible immobilization of a thermophilic \u03b2-galactosidase via ionic adsorption on PEI-coated Sepabeads","volume":"32","author":"Pessela","year":"2003","journal-title":"Enzyme and Microbial Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0180","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/(SICI)1097-0290(20000405)68:1<98::AID-BIT12>3.0.CO;2-T","article-title":"Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support\u2013polyethylenimine composites","volume":"68","author":"Mateo","year":"2000","journal-title":"Biotechnology and Bioengineering"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0185","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.enzmictec.2006.05.009","article-title":"Evaluation of different immobilization strategies to prepare an industrial biocatalyst of formate dehydrogenase from Candida boidinii","volume":"40","author":"Bolivar","year":"2007","journal-title":"Enzyme and Microbial Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0190","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.enzmictec.2006.04.021","article-title":"Stabilization of different alcohol oxidases via immobilization and post immobilization techniques","volume":"40","author":"Lopez-Gallego","year":"2007","journal-title":"Enzyme and Microbial Technology"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0195","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.molcatb.2012.05.013","article-title":"Influence of the use of Aliquat 336 in the immobilization procedure in sol\u2013gel of lipase from Bacillus sp. ITP\u2013001","volume":"84","author":"Souza","year":"2012","journal-title":"Journal of Molecular Catalysis B: Enzymatic"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0200","unstructured":"Das S. Immobilization of enzymes in sol\u2013gel mesoporous silica, enzymatic digestion of biomass, and silica\u2013curcumin hybrid materials. Thesis, the Faculty of Drexel University, March 2011."},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0205","series-title":"Adsorption","author":"Oscik","year":"1982"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0210","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0021-9673(97)00201-X","article-title":"Study of the physico-chemical properties of some packing materials: III. Pore size and surface area distribution","volume":"773","author":"Guan-Sajonz","year":"1997","journal-title":"Journal of Chromatography A"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0215","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.micromeso.2009.02.030","article-title":"Effect of nonsurfactant template content on the particle size and surface area of monodisperse mesoporous silica nanospheres","volume":"122","author":"Mukherjee","year":"2009","journal-title":"Microporous and Mesoporous Materials"},{"key":"10.1016\/j.enzmictec.2012.12.007_bib0220","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1021\/cm981004u","article-title":"Preparation and physisorption characterization of d-glucose-templated mesoporous silica materials via base-catalyzed sol\u2013gel process","volume":"11","author":"Wei","year":"1999","journal-title":"Chemistry of Materials"}],"container-title":["Enzyme and Microbial Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022912002074?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022912002074?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,7,7]],"date-time":"2019-07-07T15:02:29Z","timestamp":1562511749000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141022912002074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["S0141022912002074"],"URL":"https:\/\/doi.org\/10.1016\/j.enzmictec.2012.12.007","relation":{},"ISSN":["0141-0229"],"issn-type":[{"value":"0141-0229","type":"print"}],"subject":[],"published":{"date-parts":[[2013,3]]}}}