{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:05:48Z","timestamp":1791918348612,"version":"4.3.4"},"reference-count":80,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00c3\u00adfico e Tecnol\u00c3\u00b3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00c3\u00a7\u00c3\u00a3o de Aperfei\u00c3\u00a7oamento de Pessoal de N\u00c3\u00advel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00c3\u00adfico e Tecnol\u00c3\u00b3gico","doi-asserted-by":"publisher","award":["422942\/2016-2"],"award-info":[{"award-number":["422942\/2016-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00c3\u00adfico e Tecnol\u00c3\u00b3gico","doi-asserted-by":"publisher","award":["311062\/2019-9"],"award-info":[{"award-number":["311062\/2019-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00c3\u00adfico e Tecnol\u00c3\u00b3gico","doi-asserted-by":"publisher","award":["408790\/2016-4"],"award-info":[{"award-number":["408790\/2016-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00c3\u00a7\u00c3\u00a3o de Aperfei\u00c3\u00a7oamento de Pessoal de N\u00c3\u00advel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005283","name":"Funda\u00c3\u00a7\u00c3\u00a3o Cearense de Apoio ao Desenvolvimento Cient\u00c3\u00adfico e Tecnol\u00c3\u00b3gico","doi-asserted-by":"publisher","award":["BP3-0139-00005.01.00\/18"],"award-info":[{"award-number":["BP3-0139-00005.01.00\/18"]}],"id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005283","name":"Funda\u00c3\u00a7\u00c3\u00a3o Cearense de Apoio ao Desenvolvimento Cient\u00c3\u00adfico e Tecnol\u00c3\u00b3gico","doi-asserted-by":"publisher","award":["PNE-0112-00048.01.00\/16"],"award-info":[{"award-number":["PNE-0112-00048.01.00\/16"]}],"id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Enzyme and Microbial Technology"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.enzmictec.2020.109560","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T02:26:48Z","timestamp":1587608808000},"page":"109560","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":89,"special_numbering":"C","title":["A new heterofunctional support for enzyme immobilization: PEI functionalized Fe3O4 MNPs activated with divinyl sulfone. Application in the immobilization of lipase from Thermomyces lanuginosus"],"prefix":"10.1016","volume":"138","author":[{"given":"Rayanne M.","family":"Bezerra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolpho R.C.","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davino M. Andrade","family":"Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco F.M.","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina C.M.","family":"de Paula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Telma L.G.","family":"de Lemos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre B.A.","family":"Fechine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio A.","family":"Correa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Bohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0012-8971","authenticated-orcid":false,"given":"Luciana R.B.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1511-5180","authenticated-orcid":false,"given":"Jos\u00e9 C.S.","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.enzmictec.2020.109560_bib0005","doi-asserted-by":"crossref","first-page":"20639","DOI":"10.1039\/C4RA16926C","article-title":"Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to Chymotrypsin","volume":"5","author":"dos Santos","year":"2015","journal-title":"RSC Adv."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0010","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.molcatb.2016.08.009","article-title":"Strategies of covalent immobilization of a recombinant Candida antarctica lipase B on pore-expanded SBA-15 and its application in the kinetic resolution of (R, S)-Phenylethyl acetate","volume":"133","author":"Rios","year":"2016","journal-title":"J. Mol. Catal., B Enzym."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0015","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.bej.2017.05.024","article-title":"Design of a lipase-nano particle biocatalysts and its use in the kinetic resolution of medicament precursors","volume":"125","author":"Bezerra","year":"2017","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0020","doi-asserted-by":"crossref","first-page":"6437","DOI":"10.1039\/c3cs35506c","article-title":"Industrial use of immobilized enzymes","volume":"42","author":"DiCosimo","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0025","doi-asserted-by":"crossref","first-page":"6223","DOI":"10.1039\/C3CS60075K","article-title":"Enzyme immobilisation in biocatalysis: why, what and how","volume":"42","author":"Sheldon","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2019.09.003","article-title":"Further stabilization of lipase from Pseudomonas fluorescens immobilized on octyl coated nanoparticles via chemical modification with bifunctional agents","author":"Rios","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0035","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.procbio.2019.08.016","article-title":"Modulation of Lecitase properties via immobilization on differently activated Immobead-350: stabilization and inversion of enantiospecificity","volume":"87","author":"Pinheiro","year":"2019","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0040","doi-asserted-by":"crossref","first-page":"5807","DOI":"10.3390\/ijms20225807","article-title":"Ethyl butyrate synthesis catalyzed by lipases A and B from Candida antarcticaImmobilized onto magnetic nanoparticles. improvement of biocatalysts\u2019 performance under ultrasonic irradiation","volume":"20","author":"Monteiro","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0045","doi-asserted-by":"crossref","first-page":"4018","DOI":"10.3390\/ijms20164018","article-title":"Immobilization of lipase a from Candida antarctica onto chitosan-coated magnetic nanoparticles","volume":"20","author":"Monteiro","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0050","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.procbio.2015.11.015","article-title":"Improved immobilization and stabilization of lipase from Rhizomucor miehei on octyl-glyoxyl agarose beads by using CaCl 2","volume":"51","author":"Fernandez-Lopez","year":"2016","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0055","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.enzmictec.2005.10.014","article-title":"Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins","volume":"39","author":"Mateo","year":"2006","journal-title":"Enzyme Microb. Technol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0060","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.ijbiomac.2014.06.035","article-title":"Covalent attachment of lipases on glyoxyl-agarose beads: application in fruit flavor and biodiesel synthesis","volume":"70","author":"Mendes","year":"2014","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0065","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.cattod.2015.05.027","article-title":"Chemical amination of lipases improves their immobilization on octyl-glyoxyl agarose beads","volume":"259","author":"Rueda","year":"2016","journal-title":"Catal. Today"},{"key":"10.1016\/j.enzmictec.2020.109560_bib0070","first-page":"11212","volume":"5","author":"Rueda","year":"2015"},{"key":"10.1016\/j.enzmictec.2020.109560_bib0075","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.molcatb.2014.02.016","article-title":"Covalent immobilization of benzoylformate decarboxylase from Pseudomonas putida on magnetic epoxy support and its carboligation reactivity","volume":"102","author":"Tural","year":"2014","journal-title":"J. Mol. Catal., B Enzym."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0080","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1042\/BST0351593","article-title":"Advances in the design of new epoxy supports for enzyme immobilization-stabilization","volume":"35","author":"Mateo","year":"2007","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0085","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.apsusc.2014.03.130","article-title":"Optimization and characterization of biomolecule immobilization on silicon substrates using (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde linker","volume":"305","author":"Gunda","year":"2014","journal-title":"Appl. Surf. Sci."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0090","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/j.procbio.2012.04.019","article-title":"Versatility of glutaraldehyde to immobilize lipases: effect of the immobilization protocol on the properties of lipase B from Candida antarctica","volume":"47","author":"Barbosa","year":"2012","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0095","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1039\/C3RA45991H","article-title":"Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization","volume":"4","author":"Barbosa","year":"2014","journal-title":"RSC Adv."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0100","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1590\/0104-6632.20170343s20150575","article-title":"Production of flavor esters catalyzed by lipase B from Candida antarctica immobilized on magnetic nanoparticles","volume":"34","author":"de Souza","year":"2017","journal-title":"Braz. J. Chem. Eng."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0105","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.procbio.2017.09.020","article-title":"A new heterofunctional amino-vinyl sulfone support to immobilize enzymes: application to the stabilization of \u03b2-galactosidase from Aspergillus oryzae","volume":"64","author":"Zaak","year":"2018","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0110","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.molcatb.2015.04.008","article-title":"Bovine trypsin immobilization on agarose activated with divinylsulfone: improved activity and stability via multipoint covalent attachment","volume":"117","author":"dos Santos","year":"2015","journal-title":"J. Mol. Catal., B Enzym."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.enzmictec.2015.05.001","article-title":"Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironment","volume":"77","author":"dos Santos","year":"2015","journal-title":"Enzyme Microb. Technol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0120","doi-asserted-by":"crossref","first-page":"35801","DOI":"10.1039\/C5RA03798K","article-title":"Versatility of divinylsulfone supports permits the tuning of CALB properties during its immobilization","volume":"5","author":"dos Santos","year":"2015","journal-title":"RSC Adv."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0125","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.procbio.2015.03.018","article-title":"Evaluation of divinylsulfone activated agarose to immobilize lipases and to tune their catalytic properties","volume":"50","author":"dos Santos","year":"2015","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0130","doi-asserted-by":"crossref","first-page":"20639","DOI":"10.1039\/C4RA16926C","article-title":"Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin","volume":"5","author":"Dos Santos","year":"2015","journal-title":"RSC Adv."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0135","doi-asserted-by":"crossref","DOI":"10.1016\/0167-4838(93)90160-S","article-title":"The anaerobic reaction of bovine hemoglobin with divinyl sulfone: structural changes and functional consequences","author":"Ilan","year":"1993","journal-title":"Biochim. Biophys. Acta (BBA)\/Protein Struct. Mol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0140","doi-asserted-by":"crossref","DOI":"10.1039\/B920576D","article-title":"Vinyl sulfone: a versatile function for simple bioconjugation and immobilization","author":"Morales-Sanfrutos","year":"2010","journal-title":"Org. Biomol. Chem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0145","doi-asserted-by":"crossref","first-page":"7189","DOI":"10.1039\/c0jm00720j","article-title":"Vinyl sulfone functionalized silica: a \u201cready to use\u201d pre-activated material for immobilization of biomolecules","volume":"20","author":"Ortega-Mu\u00f1oz","year":"2010","journal-title":"J. Mater. Chem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0150","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.ijbiomac.2019.02.145","article-title":"Chitosan activated with divinyl sulfone: a new heterofunctional support for enzyme immobilization. Application in the immobilization of lipase B from Candida antarctica","volume":"130","author":"Pinheiro","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0155","doi-asserted-by":"crossref","first-page":"7461","DOI":"10.1039\/C7TB01639E","article-title":"Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts","volume":"5","author":"Virgen-Ort\u00edz","year":"2017","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.enzmictec.2020.109560_bib0160","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.procbio.2016.04.002","article-title":"Easy stabilization of interfacially activated lipases using heterofunctional divinyl sulfone activated-octyl agarose beads. Modulation of the immobilized enzymes by altering their nanoenvironment","volume":"51","author":"Albuquerque","year":"2016","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0165","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s10529-015-1977-z","article-title":"Application of magnetic nanoparticles in smart enzyme immobilization","volume":"38","author":"Vaghari","year":"2016","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0170","doi-asserted-by":"crossref","first-page":"3445","DOI":"10.2147\/IJN.S30320","article-title":"Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers","volume":"7","author":"Wahajuddin","year":"2012","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0175","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0154445","article-title":"Relating the surface properties of superparamagnetic iron oxide nanoparticles (SPIONs) to their bactericidal effect towards a biofilm of Streptococcus mutans","volume":"11","author":"Javanbakht","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.enzmictec.2020.109560_bib0180","doi-asserted-by":"crossref","first-page":"139","DOI":"10.4028\/www.scientific.net\/SSP.241.139","article-title":"Super-paramagnetic nanoparticles with spinel structure: a review of synthesis and biomedical applications","volume":"241","author":"Galv\u00e3o","year":"2015","journal-title":"Solid State Phenom."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0185","doi-asserted-by":"crossref","first-page":"24206","DOI":"10.1021\/acs.jpcc.7b04941","article-title":"Rapid sonochemical approach produces functionalized Fe3O4 nanoparticles with excellent magnetic, colloidal, and relaxivity properties for MRI application","volume":"121","author":"Neto","year":"2017","journal-title":"J. Phys. Chem. C."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0190","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.chemosphere.2018.07.192","article-title":"Antioxidative response of Phanerochaete chrysosporium against silver nanoparticle-induced toxicity and its potential mechanism","volume":"211","author":"Huang","year":"2018","journal-title":"Chemosphere."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0195","doi-asserted-by":"crossref","first-page":"2803","DOI":"10.1007\/s11051-014-2803-6","article-title":"Cubic superparamagnetic nanoparticles of NiFe2O4 via fast microwave heating","volume":"16","author":"Galv\u00e3o","year":"2014","journal-title":"J. Nanopart. Res."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0200","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1021\/am302883m","article-title":"Facile hydrothermal synthesis and surface functionalization of polyethyleneimine-coated iron oxide nanoparticles for biomedical applications","volume":"5","author":"Cai","year":"2013","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.enzmictec.2020.109560_bib0205","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/(SICI)1097-0290(20000405)68:1<98::AID-BIT12>3.0.CO;2-T","article-title":"Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites","volume":"68","author":"Mateo","year":"2000","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0210","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.molcatb.2009.11.010","article-title":"Lipase from Thermomyces lanuginosus: uses and prospects as an industrial biocatalyst","volume":"62","author":"Fernandez-Lafuente","year":"2010","journal-title":"J. Mol. Catal., B Enzym."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0215","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1021\/mp500656v","article-title":"Smart polymeric nanoparticles for cancer gene delivery","volume":"12","author":"Lin","year":"2015","journal-title":"Mol. Pharm."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0220","doi-asserted-by":"crossref","first-page":"7629","DOI":"10.3390\/molecules19067629","article-title":"Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases","volume":"19","author":"Garcia-Galan","year":"2014","journal-title":"Molecules."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0225","doi-asserted-by":"crossref","DOI":"10.1016\/j.molcatb.2016.05.007","article-title":"Cashew apple bagasse as a support for the immobilization of lipase B from Candida antarctica: application to the chemoenzymatic production of (R)-Indanol","author":"de Souza","year":"2016","journal-title":"J. Mol. Catal., B Enzym."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0230","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0235","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.btre.2017.02.003","article-title":"Immobilization of CALB on activated chitosan: application to enzymatic synthesis in supercritical and near-critical carbon dioxide","volume":"14","author":"dos Santos","year":"2017","journal-title":"Biotechnol. Rep."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0240","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1007\/s12010-017-2622-1","article-title":"Effect of the presence of surfactants and immobilization conditions on catalysts\u2019 properties of rhizomucor miehei lipase onto chitosan","volume":"184","author":"de Oliveira","year":"2018","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0245","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.colsurfa.2013.12.026","article-title":"Synthesis of polyethylenimine modified Fe3O4 nanoparticles with immobilized Cu2+ for highly efficient proteins adsorption","volume":"443","author":"Xia","year":"2014","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0250","doi-asserted-by":"crossref","first-page":"4012","DOI":"10.1016\/j.biomaterials.2008.07.004","article-title":"Magnetic nanoparticles with dual functional properties: drug delivery and magnetic resonance imaging","volume":"29","author":"Jain","year":"2008","journal-title":"Biomaterials."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0255","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.enzmictec.2020.109560_bib0260","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/j.procbio.2014.01.028","article-title":"Tuning of Lecitase features via solid-phase chemical modification: effect of the immobilization protocol","volume":"49","author":"Garcia-Galan","year":"2014","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0265","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1111\/j.1749-6632.1987.tb45687.x","article-title":"Analysis of enzyme deactivations by a series-type mechanism: influence of modification on the activity and stability of enzymes","volume":"501","author":"Sadana","year":"1987","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0270","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.3390\/molecules22122165","article-title":"Synthesis of benzyl acetate catalyzed by lipase immobilized in nontoxic chitosan-polyphosphate beads","volume":"22","author":"Melo","year":"2017","journal-title":"Molecules"},{"key":"10.1016\/j.enzmictec.2020.109560_bib0275","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2019.06.062","article-title":"Evaluation of zeta potential of nanomaterials by electrophoretic light scattering: fast field reversal versus slow field reversal modes","volume":"205","author":"Varenne","year":"2019","journal-title":"Talanta."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0280","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.jcis.2006.12.075","article-title":"Measurement and interpretation of electrokinetic phenomena","volume":"309","author":"Delgado","year":"2007","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0285","doi-asserted-by":"crossref","DOI":"10.1351\/pac200577101753","article-title":"Measurement and interpretation of electrokinetic phenomena: (IUPAC technical report)","author":"Delgado","year":"2005","journal-title":"Pure Appl. Chem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0290","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1021\/bc049895l","article-title":"Amphiphilic core\u2212shell nanoparticles with poly(ethylenimine) shells as potential gene delivery carriers","volume":"16","author":"Zhu","year":"2005","journal-title":"Bioconjug. Chem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0295","doi-asserted-by":"crossref","first-page":"6250","DOI":"10.1039\/c3cs35495d","article-title":"Conformational changes of enzymes upon immobilisation","volume":"42","author":"Secundo","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0300","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1021\/acssuschemeng.5b00869","article-title":"A novel approach to enzymatic unhairing and fiber opening of skin using enzymes immobilized on magnetite nanoparticles","volume":"4","author":"Murugappan","year":"2016","journal-title":"ACS Sustain. Chem. Eng."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0305","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.ijbiomac.2017.01.023","article-title":"Covalent immobilization of trypsin onto modified magnetite nanoparticles and its application for casein digestion","volume":"97","author":"Atacan","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0310","doi-asserted-by":"crossref","DOI":"10.1021\/ja026855o","article-title":"Entrapping enzyme in a functionalized nanoporous support","author":"Lei","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0315","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.matchemphys.2011.04.083","article-title":"Ultrasonic-assisted synthesis of magnetite based MRI contrast agent using cysteine as the biocapping coating","volume":"131","author":"Ahmadi","year":"2011","journal-title":"Mater. Chem. Phys."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0320","doi-asserted-by":"crossref","first-page":"7605","DOI":"10.1016\/j.ceramint.2013.03.015","article-title":"Synthesis of high magnetization hydrophilic magnetite (Fe3O4) nanoparticles in single reaction\u2014surfactantless polyol process","volume":"39","author":"Abbas","year":"2013","journal-title":"Ceram. Int."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0325","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.carbpol.2013.09.060","article-title":"Relationship between structure and cytocompatibility of divinyl sulfone cross-linked hyaluronic acid","volume":"101","author":"Lai","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0330","series-title":"Vibrational Frequencies and Structural Determinations of Divinyl Sulfoxide","author":"Ellzy","year":"2004"},{"key":"10.1016\/j.enzmictec.2020.109560_bib0335","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.bej.2005.01.007","article-title":"Effect of the chain length of the acid on the enzymatic synthesis of flavors in supercritical carbon dioxide","volume":"23","author":"Kumar","year":"2005","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0340","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.biortech.2015.09.028","article-title":"Synthesis of benzyl cinnamate by enzymatic esterification of cinnamic acid","volume":"198","author":"Wang","year":"2015","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0345","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.enzmictec.2016.04.007","article-title":"Operational stabilities of different chemical derivatives of Novozym 435 in an alcoholysis reaction","volume":"90","author":"Villalba","year":"2016","journal-title":"Enzyme Microb. Technol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0350","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.bej.2016.03.004","article-title":"Effect of chemical modification of Novozym 435 on its performance in the alcoholysis of camelina oil","volume":"111","author":"Verdasco-Mart\u00edn","year":"2016","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0355","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1016\/j.procbio.2015.06.023","article-title":"Accurel M.P. 1000 as a support for the immobilization of lipase from Burkholderia cepacia : Application to the kinetic resolution of myo -inositol derivatives","volume":"50","author":"Manoel","year":"2015","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0360","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1039\/B918763B","article-title":"Green chemistry: principles and practice","volume":"39","author":"Anastas","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0365","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1038\/35051736","article-title":"Industrial biocatalysis today and tomorrow","volume":"409","author":"Schmid","year":"2001","journal-title":"Nature."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0370","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.tibtech.2006.12.005","article-title":"Biocatalysis for pharmaceutical intermediates: the future is now","volume":"25","author":"Pollard","year":"2007","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0375","doi-asserted-by":"crossref","DOI":"10.1016\/S0958-1669(99)80060-3","article-title":"Crosslinking of enzymes for improved stability and performance","author":"Govardhan","year":"1999","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0380","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1016\/j.procbio.2014.05.009","article-title":"Stabilizing hyperactivated lecitase structures through physical treatment with ionic polymers","volume":"49","author":"dos Santos","year":"2014","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0385","doi-asserted-by":"crossref","first-page":"43989","DOI":"10.1039\/C6RA02129H","article-title":"Role of functionalization: strategies to explore potential nano-bio applications of magnetic nanoparticles","volume":"6","author":"Bohara","year":"2016","journal-title":"RSC Adv."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0390","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/elsc.201100225","article-title":"Enzyme stabilization by nano\/microsized hybrid materials","volume":"13","author":"Hwang","year":"2013","journal-title":"Eng. Life Sci."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0395","doi-asserted-by":"crossref","first-page":"2885","DOI":"10.1002\/adsc.201100534","article-title":"Potential of different enzyme immobilization strategies to improve enzyme performance","volume":"353","author":"Garcia-Galan","year":"2011","journal-title":"Adv. Synth. Catal."},{"key":"10.1016\/j.enzmictec.2020.109560_bib0400","doi-asserted-by":"crossref","first-page":"14121","DOI":"10.1007\/s10853-018-2641-5","article-title":"Novel nanohybrid biocatalyst: application in the kinetic resolution of secondary alcohols","volume":"53","author":"Galv\u00e3o","year":"2018","journal-title":"J. Mater. Sci."}],"container-title":["Enzyme and Microbial Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022920300533?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022920300533?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T01:40:55Z","timestamp":1758850855000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141022920300533"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":80,"alternative-id":["S0141022920300533"],"URL":"https:\/\/doi.org\/10.1016\/j.enzmictec.2020.109560","relation":{},"ISSN":["0141-0229"],"issn-type":[{"value":"0141-0229","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A new heterofunctional support for enzyme immobilization: PEI functionalized Fe3O4 MNPs activated with divinyl sulfone. Application in the immobilization of lipase from Thermomyces lanuginosus","name":"articletitle","label":"Article Title"},{"value":"Enzyme and Microbial Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.enzmictec.2020.109560","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109560"}}