{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:19:16Z","timestamp":1788445156760,"version":"build-2803163510"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["DBT\/2021-22\/GUJUN\/1491"],"award-info":[{"award-number":["DBT\/2021-22\/GUJUN\/1491"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Enzyme and Microbial Technology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.enzmictec.2026.110946","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T06:41:14Z","timestamp":1785307274000},"page":"110946","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Purification and kinetic characterization of cyclodextrin glycosyltransferase from Alkalihalobacillus Trypoxylicola KRM"],"prefix":"10.1016","volume":"201","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6495-6827","authenticated-orcid":false,"given":"Khushbu","family":"Rabadiya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3206-248X","authenticated-orcid":false,"given":"Dimple","family":"Pardhi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8828-0136","authenticated-orcid":false,"given":"Khushali","family":"Thaker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6544-3564","authenticated-orcid":false,"given":"Jaimini","family":"Patoliya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8669-8347","authenticated-orcid":false,"given":"Kiransinh","family":"Rajput","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3772-5569","authenticated-orcid":false,"given":"Rushikesh","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.enzmictec.2026.110946_bib1","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1002\/jobm.201800390","article-title":"Production and characterization of \u03b2-cyclodextrin glucanotransferase from Bacillus sp. ND1","volume":"59","author":"Upadhyay","year":"2018","journal-title":"J. Basic. Microbiol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib2","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1007\/s10311-018-0763-2","article-title":"Cyclodextrins, from molecules to applications","volume":"16","author":"Crini","year":"2018","journal-title":"Environ. Chem. Lett."},{"key":"10.1016\/j.enzmictec.2026.110946_bib3","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-023-12708-9","article-title":"Cyclodextrin glucanotransferase: fundamentals and biotechnological implications","author":"Pardhi","year":"2023","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2024.129315","article-title":"A review on recent upgradation and strategies to enhance cyclodextrin glucanotransferase properties for its applications","volume":"259","author":"Rabadiya","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib5","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.procbio.2024.07.008","article-title":"Current advances in obtaining novel cyclodextrin glycosyltransferases for optimizing the synthesis of cyclodextrins","volume":"145","author":"de","year":"2024","journal-title":"Process. Biochem."},{"key":"10.1016\/j.enzmictec.2026.110946_bib6","first-page":"41","article-title":"Purification and Characterization of a Cyclomaltodextrin Glucanotransferase From Paenibacillus campinasensis Strain H69-3","volume":"137","author":"Alves-Prado","year":"2007","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib7","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1271\/bbb.58.1968","article-title":"Purification and Properties of Cyclodextrin Glucanotransferase from Brevibacterium sp. No. 9605","volume":"58","author":"Mori","year":"1994","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.enzmictec.2026.110946_bib8","first-page":"1","article-title":"Screening and Selection of Medium Components for Cyclodextrin Glucanotransferase Production by New Alkaliphile Microbacterium terrae KNR 9 Using Plackett-Burman Design","volume":"2016","author":"Rajput","year":"2016","journal-title":"Biotechnol. Res. Int."},{"key":"10.1016\/j.enzmictec.2026.110946_bib9","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1002\/(SICI)1097-0290(19990720)64:2<168::AID-BIT5>3.0.CO;2-5","article-title":"Application of factorial designs for optimization of cyclodextrin glycosyltransferase production from Klebsiella pneumoniae pneumoniae AS-22","volume":"64","author":"Gawande","year":"1999","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.enzmictec.2026.110946_bib10","first-page":"143","article-title":"Optimization and characterization of cyclodextrin glycosyl- transferase produced by Thermoactinomyces vulgaris TA1","volume":"14","author":"Alsharawi","year":"2018","journal-title":"Egypt. J. Exp. Biol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib11","doi-asserted-by":"crossref","first-page":"7402","DOI":"10.1021\/acs.jafc.8b02143","article-title":"Efficient \u03b1-Glucosylation of Epigallocatechin Gallate Catalyzed by Cyclodextrin Glucanotransferase from Thermoanaerobacter Species","volume":"66","author":"Gonzalez-Alfonso","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.enzmictec.2026.110946_bib12","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1128\/JB.184.3.777-784.2002","article-title":"Characterization of an archaeal cyclodextrin glucanotransferase with a novel C-terminal domain","volume":"184","author":"Rashid","year":"2002","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib13","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1080\/10826068.2024.2386558","article-title":"The improved purification technique for isolation of the novel CGTase from the alkaliphilc strain Caldalkalibacillus mannanilyticus IB-OR17-B1","volume":"55","author":"Yu. Milman","year":"2025","journal-title":"Prep. Biochem. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib14","article-title":"Purification and characterisation of cyclodextrin glucanotransferase by Paenibacillus daejeonensis P-83","volume":"1","author":"Pardhi","year":"2025","journal-title":"Bioeng"},{"key":"10.1016\/j.enzmictec.2026.110946_bib15","doi-asserted-by":"crossref","first-page":"121","DOI":"10.5458\/jag.57.121","article-title":"Characterization of Two \u03b3-Cyclodextrin-specific Enzymes from Bacillus clarkii 7364","volume":"57","author":"Nakagawa","year":"2010","journal-title":"J. Appl. Glycosci."},{"key":"10.1016\/j.enzmictec.2026.110946_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/2193-1801-1-61","article-title":"Partial purification and properties of cyclodextrin glycosiltransferase (CGTase) from alkalophilic Bacillus species","volume":"1","author":"Mora","year":"2012","journal-title":"Springerplus"},{"key":"10.1016\/j.enzmictec.2026.110946_bib17","first-page":"1227","article-title":"Purification and Characterization of Cyclodextrin Glycosyltransferase From Alkaliphilic Bacillus Oshimensis","volume":"5","author":"Pol","year":"2016","journal-title":"World J. Pharm. Res."},{"key":"10.1016\/j.enzmictec.2026.110946_bib18","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s00449-014-1318-y","article-title":"Purification and characterization of cyclodextrin \u03b2-glucanotransferase from novel alkalophilic bacilli","volume":"38","author":"Elbaz","year":"2015","journal-title":"Bioprocess. Biosyst. Eng."},{"key":"10.1016\/j.enzmictec.2026.110946_bib19","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.enzmictec.2026.110946_bib20","doi-asserted-by":"crossref","unstructured":"X. Jia, X. Ye, J. Chen, X. Lin, L. Vasseur, M. YouPurification and biochemical characterization of a cyclodextrin glycosyltransferase from Geobacillus thermoglucosidans CHB1, Starch\/Staerke. 70 (2018) 1\u201311. 10.1002\/star.2017000162018.","DOI":"10.1002\/star.201700016"},{"key":"10.1016\/j.enzmictec.2026.110946_bib21","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3923\/biotech.2015.181.187","article-title":"D.S. Retnoningrum, Y93F substitution of cyclodextrin glucanotransferase from Bacillus sp. A2-5a and its enzyme characterization","volume":"14","author":"Rostinawati","year":"2015","journal-title":"Biotechnology"},{"key":"10.1016\/j.enzmictec.2026.110946_bib22","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.02453-24","article-title":"Proof-of-concept MALDI-TOF-MS assay for the detection of Toxin B enzymatic activity in Clostridioides difficile infection","volume":"13","author":"Dvorak","year":"2025","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.enzmictec.2026.110946_bib23","doi-asserted-by":"crossref","first-page":"109","DOI":"10.56899\/152.01.08","article-title":"Alkalihalobacillus lehensis M136, a Novel Alkaliphilic, Cyclodextrin Glucanotransferase (CGTase)-producing Isolate from Manleluag Hyperalkaline Spring in Pangasinan, Philippines","volume":"152","author":"Bosito","year":"2023","journal-title":"Philipp. J. Sci."},{"key":"10.1016\/j.enzmictec.2026.110946_bib24","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/S0141-0229(03)00215-1","article-title":"Bacillus agaradhaerens LS-3C cyclodextrin glycosyltransferase: Activity and stability features","volume":"33","author":"Martins","year":"2003","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib25","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1021\/jf970707d","article-title":"Characterization of a Thermostable Cyclodextrin Glucanotransferase Isolated from Bacillus stearothermophilus ET1","volume":"46","author":"Chung","year":"1998","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.enzmictec.2026.110946_bib26","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/S0141-0229(01)00461-6","article-title":"A new cyclodextrin glycosyltransferase from an alkaliphilic Bacillus agaradhaerens isolate: Purification and characterisation","volume":"30","author":"Martins","year":"2002","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib27","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.procbio.2010.07.027","article-title":"A novel cyclodextrin glucanotransferase from alkaliphilic Bacillus pseudalcaliphilus 20RF: Purification and properties","volume":"46","author":"Atanasova","year":"2011","journal-title":"Process. Biochem."},{"key":"10.1016\/j.enzmictec.2026.110946_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s13205-016-0495-6","article-title":"A novel cyclodextrin glucanotransferase from an alkaliphile Microbacterium terrae KNR 9: purification and properties","volume":"6","author":"Rajput","year":"2016","journal-title":"3 Biotech"},{"key":"10.1016\/j.enzmictec.2026.110946_bib29","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.fbio.2018.10.006","article-title":"Enhancement of \u03b1-CGTase thermostability with the addition of calcium or barium ions","volume":"26","author":"Li","year":"2018","journal-title":"Food Biosci."},{"key":"10.1016\/j.enzmictec.2026.110946_bib30","article-title":"The cyclodextrin glycosyltransferase of Paenibacillus pabuli US132 strain: molecular characterization and overproduction of the recombinant enzyme","volume":"2008","author":"Jemli","year":"2008","journal-title":"J. Biomed. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib31","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/s13213-011-0266-x","article-title":"Bacillus lehensis - An alkali-tolerant bacterium isolated from cassava starch wastewater: Optimization of parameters for cyclodextrin glycosyltransferase production","volume":"62","author":"Blanco","year":"2012","journal-title":"Ann. Microbiol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib32","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1007\/s12010-011-9346-4","article-title":"Purification and Properties of a New Thermostable Cyclodextrin Glucanotransferase from Bacillus pseudalcaliphilus 8SB","volume":"165","author":"Kitayska","year":"2011","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib33","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1002\/jobm.201700270","article-title":"Purification and properties of beta-cyclomaltodextrin glucanotransferase from Bacillus flexus SV 1","volume":"57","author":"Reddy","year":"2017","journal-title":"J. Basic. Microbiol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib34","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/0922-338X(91)90344-G","article-title":"Purification and properties of Bacillus coagulans cyclomaltodextrin glucanotransferase","volume":"71","author":"Akimaru","year":"1991","journal-title":"J. Ferment. Bioeng."},{"key":"10.1016\/j.enzmictec.2026.110946_bib35","doi-asserted-by":"crossref","first-page":"10505","DOI":"10.3390\/ijms130810505","article-title":"A novel cyclodextrin glycosyltransferase from alkaliphilic Amphibacillus sp. NPST-10: Purification and properties","volume":"13","author":"Ibrahim","year":"2012","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.enzmictec.2026.110946_bib36","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1271\/bbb.67.334","article-title":"Isolation of Paenibacillus illinoisensis that produces cyclodextrin glucanotransferase resistant to organic solvents","volume":"67","author":"Doukyu","year":"2003","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.enzmictec.2026.110946_bib37","article-title":"Surface engineering of cyclodextrin glycosyltransferase reveals structural compactness and rigidity responsible for enhanced organic solvents resistance","volume":"550","author":"Han","year":"2023","journal-title":"Mol. Catal."},{"key":"10.1016\/j.enzmictec.2026.110946_bib38","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1007\/s002530051424","article-title":"Purification and properties of a novel raw starch degrading cyclomaltodextrin glucanotransferase from Bacillus firmus","volume":"51","author":"Gawande","year":"1999","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12896-018-0463-9","article-title":"Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-\u03b1-D-glucopyranosyl-L-ascorbic acid production","volume":"18","author":"Jiang","year":"2018","journal-title":"BMC Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110946_bib40","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.procbio.2017.04.041","article-title":"Directed immobilization of CGTase: The effect of the enzyme orientation on the enzyme activity and its use in packed-bed reactor for continuous production of cyclodextrins","volume":"58","author":"Sch\u00f6ffer","year":"2017","journal-title":"Process. Biochem."},{"key":"10.1016\/j.enzmictec.2026.110946_bib41","doi-asserted-by":"crossref","first-page":"4241","DOI":"10.1021\/bi952339h","article-title":"Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 \u00c5 resolution. Implications for product specificity","volume":"35","author":"Strokopytov","year":"1996","journal-title":"Biochemistry"},{"key":"10.1016\/j.enzmictec.2026.110946_bib42","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1016\/j.procbio.2004.03.018","article-title":"Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1","volume":"40","author":"Kek","year":"2005","journal-title":"Process. Biochem."},{"key":"10.1016\/j.enzmictec.2026.110946_bib43","doi-asserted-by":"crossref","DOI":"10.1007\/s13205-022-03111-8","article-title":"Molecular cloning, and optimized production and characterization of recombinant cyclodextrin glucanotransferase from Bacillus sp. T1","volume":"12","author":"Liu","year":"2022","journal-title":"3 Biotech"},{"key":"10.1016\/j.enzmictec.2026.110946_bib44","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.molcatb.2008.09.016","article-title":"A predominant \u03b2-CGTase G1 engineered to elucidate the relationship between protein structure and product specificity","volume":"57","author":"Goh","year":"2009","journal-title":"J. Mol. Catal. B Enzym."},{"key":"10.1016\/j.enzmictec.2026.110946_bib45","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1046\/j.1432-1327.2000.01031.x","article-title":"The three transglycosylation reactions catalyzed by cyclodextrin glycosyltransferase from Bacillus circulans (strain 251) proceed via different kinetic mechanisms","volume":"267","author":"Van Der Veen","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.enzmictec.2026.110946_bib46","doi-asserted-by":"crossref","first-page":"48","DOI":"10.17221\/432\/2012-CJFS","article-title":"Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme","volume":"32","author":"Blanco","year":"2014","journal-title":"Czech J. Food Sci."},{"key":"10.1016\/j.enzmictec.2026.110946_bib47","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0008-6215(98)00178-5","article-title":"Purification and characterisation of cyclodextrin glycosyltransferase from Paenibacillus sp. F8","volume":"310","author":"Larsen","year":"1998","journal-title":"Carbohydr. Res."}],"container-title":["Enzyme and Microbial Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022926001298?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022926001298?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:01:08Z","timestamp":1788444068000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141022926001298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":47,"alternative-id":["S0141022926001298"],"URL":"https:\/\/doi.org\/10.1016\/j.enzmictec.2026.110946","relation":{},"ISSN":["0141-0229"],"issn-type":[{"value":"0141-0229","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Purification and kinetic characterization of cyclodextrin glycosyltransferase from Alkalihalobacillus Trypoxylicola KRM","name":"articletitle","label":"Article Title"},{"value":"Enzyme and Microbial Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.enzmictec.2026.110946","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110946"}}