{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:19:41Z","timestamp":1788445181819,"version":"build-2803163510"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024MD763929"],"award-info":[{"award-number":["2024MD763929"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013099","name":"Scientific Research Fund of Liaoning Provincial Education Department","doi-asserted-by":"publisher","award":["LJ212410152045"],"award-info":[{"award-number":["LJ212410152045"]}],"id":[{"id":"10.13039\/501100013099","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013099","name":"Scientific Research Fund of Liaoning Provincial Education Department","doi-asserted-by":"publisher","award":["LJ222510152002"],"award-info":[{"award-number":["LJ222510152002"]}],"id":[{"id":"10.13039\/501100013099","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Enzyme and Microbial Technology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.enzmictec.2026.110954","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T15:05:37Z","timestamp":1786374337000},"page":"110954","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Combining a chitosan-immobilized phospholipase D with a mixed micelle system for highly efficient synthesis of phosphatidylserine"],"prefix":"10.1016","volume":"201","author":[{"given":"Zhicen","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caiyun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjian","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.enzmictec.2026.110954_bib1","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.gaost.2023.11.001","article-title":"Phosphatidylserine: an overview on functionality, processing techniques, patents, and prospects","volume":"6","author":"Chen","year":"2023","journal-title":"Grain Oil Sci. Technol."},{"key":"10.1016\/j.enzmictec.2026.110954_bib2","doi-asserted-by":"crossref","first-page":"e0164153","DOI":"10.1371\/journal.pone.0164153","article-title":"Identification of biomarkers of impaired sensory profiles among autistic patients","volume":"11","author":"El-Ansary","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.enzmictec.2026.110954_bib3","doi-asserted-by":"crossref","first-page":"466","DOI":"10.3390\/metabo11070466","article-title":"Untargeted plasma metabolomic profiling in patients with major depressive disorder using ultra-high performance liquid chromatography coupled with mass spectrometry","volume":"11","author":"Homorogan","year":"2021","journal-title":"Metabolites"},{"key":"10.1016\/j.enzmictec.2026.110954_bib4","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1476-511X-10-63","article-title":"Impaired plasma phospholipids and relative amounts of essential polyunsaturated fatty acids in autistic patients from Saudi Arabia","volume":"10","author":"El-Ansary","year":"2011","journal-title":"Lipids Health Dis."},{"key":"10.1016\/j.enzmictec.2026.110954_bib5","doi-asserted-by":"crossref","first-page":"e0226073","DOI":"10.1371\/journal.pone.0226073","article-title":"Isobaric mass tagging and triple quadrupole mass spectrometry to determine lipid biomarker candidates for Alzheimer's disease","volume":"14","author":"Tokuoka","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.enzmictec.2026.110954_bib6","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1002\/ejlt.200390074","article-title":"Toolbox for exchanging constituent fatty acids in lecithins","volume":"105","author":"Doig","year":"2003","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"10.1016\/j.enzmictec.2026.110954_bib7","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.jbiosc.2013.03.008","article-title":"Phospholipase D as a catalyst: application in phospholipid synthesis, molecular structure and protein engineering","volume":"116","author":"Damnjanovi\u0107","year":"2013","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.enzmictec.2026.110954_bib8","doi-asserted-by":"crossref","first-page":"5042","DOI":"10.1007\/s12010-025-05278-1","article-title":"A new phospholipase D-producing Bacillus cereus: taxonomy, mutagenesis, fermentation optimization and enzyme characterization","volume":"197","author":"He","year":"2025","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110954_bib9","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1021\/am5065156","article-title":"Self-assembly of amphiphilic Janus particles into monolayer capsules for enhanced enzyme catalysis in organic media","volume":"7","author":"Cao","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.enzmictec.2026.110954_bib10","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2024.1478198","article-title":"Magnetic protein aggregates generated by supramolecular assembly of ferritin cages-a modular strategy for the immobilization of enzymes","volume":"12","author":"\u00d6l\u00e7\u00fcc\u00fc","year":"2024","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110954_bib11","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.ijpharm.2013.12.015","article-title":"Natural and synthetic polymers for wounds and burns dressing","volume":"463","author":"Mogo\u015fanu","year":"2014","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.enzmictec.2026.110954_bib12","doi-asserted-by":"crossref","first-page":"3256","DOI":"10.3390\/polym13193256","article-title":"Chitosan: An overview of its properties and applications","volume":"13","author":"Aranaz","year":"2021","journal-title":"Polymers"},{"key":"10.1016\/j.enzmictec.2026.110954_bib13","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.enzmictec.2010.10.003","article-title":"Control of protein immobilization: coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance","volume":"48","author":"Hernandez","year":"2011","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.enzmictec.2026.110954_bib14","doi-asserted-by":"crossref","first-page":"92","DOI":"10.3390\/catal8020092","article-title":"A general overview of support materials for enzyme immobilization: characteristics, properties, practical utility","volume":"8","author":"Zdarta","year":"2018","journal-title":"Catalysts"},{"key":"10.1016\/j.enzmictec.2026.110954_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2024.109499","article-title":"Enhanced stability and catalytic performance of immobilized phospholipase D on chitosan-encapsulated magnetic nanoparticles using oxidized dextran and glutaraldehyde as cross-linkers","volume":"212","author":"Liu","year":"2024","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.enzmictec.2026.110954_bib16","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1002\/jctb.4732","article-title":"Stability and catalytic properties of lipase immobilized on chitosan encapsulated magnetic nanoparticles cross\u00a9\\linked with genipin and glutaraldehyde","volume":"91","author":"Liu","year":"2016","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110954_bib17","doi-asserted-by":"crossref","first-page":"571","DOI":"10.3390\/catal15060571","article-title":"Recent advances in enzyme immobilization: the role of artificial intelligence, novel nanomaterials, and dynamic carrier systems","volume":"15","author":"Tadesse","year":"2025","journal-title":"Catalysts"},{"key":"10.1016\/j.enzmictec.2026.110954_bib18","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1021\/jf505296k","article-title":"Introducing biobased ionic liquids as the nonaqueous media for enzymatic synthesis of phosphatidylserine","volume":"63","author":"Bi","year":"2015","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.enzmictec.2026.110954_bib19","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1039\/c2gc35092k","article-title":"Highly efficient synthesis of phosphatidylserine in the eco-friendly solvent \u03b3-valerolactone","volume":"14","author":"Duan","year":"2012","journal-title":"Green. Chem."},{"key":"10.1016\/j.enzmictec.2026.110954_bib20","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jbiotec.2012.10.022","article-title":"Efficient synthesis of phosphatidylserine in 2-methyltetrahydrofuran","volume":"163","author":"Duan","year":"2013","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110954_bib21","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.catcom.2016.04.010","article-title":"Insight into enzymatic synthesis of phosphatidylserine in deep eutectic solvents","volume":"82","author":"Yang","year":"2016","journal-title":"Catal. Commun."},{"key":"10.1016\/j.enzmictec.2026.110954_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2022.133865","article-title":"Cellulose nanofibril-stabilized Pickering emulsion as a high-performance interfacial biocatalysis system for the synthesis of phosphatidylserine","volume":"399","author":"Yin","year":"2023","journal-title":"Food Chem."},{"key":"10.1016\/j.enzmictec.2026.110954_bib23","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.biotechadv.2005.02.001","article-title":"Enzymatic modification of phospholipids for functional applications and human nutrition","volume":"23","author":"Guo","year":"2005","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.enzmictec.2026.110954_bib24","article-title":"A spotlight on alkaloid nanoformulations for the treatment of lung cancer","volume":"12","author":"Sm","year":"2022","journal-title":"Front. Oncol."},{"key":"10.1016\/j.enzmictec.2026.110954_bib25","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s40201-022-00842-x","article-title":"Design and preparation of amino-functionalized core-shell magnetic nanoparticles for photocatalytic application and investigation of cytotoxicity effects","volume":"21","author":"Sabouri","year":"2023","journal-title":"J. Environ. Health Sci. Eng."},{"key":"10.1016\/j.enzmictec.2026.110954_bib26","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1002\/bab.2010","article-title":"Phytosynthesis of CuONPs via Laurus nobilis: determination of antioxidant content, antibacterial activity, and dye decolorization potential","volume":"68","author":"Bulut Kocabas","year":"2021","journal-title":"Biotechnol. Appl. Biochem."},{"key":"10.1016\/j.enzmictec.2026.110954_bib27","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1080\/01496395.2011.618171","article-title":"Preparation and characterization of magnetic chitosan microspheres for endotoxin adsorption","volume":"47","author":"Yi","year":"2012","journal-title":"Sep. Sci. Technol."},{"key":"10.1016\/j.enzmictec.2026.110954_bib28","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1038\/s41558-020-0816-y","article-title":"Learning from successes and failures","volume":"10","author":"Kl\u00f6ck","year":"2020","journal-title":"Nat. Clim. Change"},{"key":"10.1016\/j.enzmictec.2026.110954_bib29","doi-asserted-by":"crossref","first-page":"6223","DOI":"10.1039\/C3CS60075K","article-title":"Enzyme immobilisation in biocatalysis: why, what and how","volume":"42","author":"Sheldon","year":"2013","journal-title":"Chem. Soc. Rev."}],"container-title":["Enzyme and Microbial Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022926001377?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022926001377?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:02:30Z","timestamp":1788444150000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141022926001377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":29,"alternative-id":["S0141022926001377"],"URL":"https:\/\/doi.org\/10.1016\/j.enzmictec.2026.110954","relation":{},"ISSN":["0141-0229"],"issn-type":[{"value":"0141-0229","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Combining a chitosan-immobilized phospholipase D with a mixed micelle system for highly efficient synthesis of phosphatidylserine","name":"articletitle","label":"Article Title"},{"value":"Enzyme and Microbial Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.enzmictec.2026.110954","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110954"}}