{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:59:50Z","timestamp":1791950390394,"version":"4.3.4"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["1550"],"award-info":[{"award-number":["1550"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["PE00000003"],"award-info":[{"award-number":["PE00000003"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100031969","name":"Solvay Specialty Polymers","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100031969","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology and Pharmacology"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.etap.2025.104799","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T15:03:55Z","timestamp":1755961435000},"page":"104799","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Evaluation of the effects of PFCAs on spheroid thyroid cells in primary culture: Effects on thyroid function-related genes, tumorigenesis and oxidative stress"],"prefix":"10.1016","volume":"119","author":[{"given":"Francesca","family":"Coperchini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Greco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Franchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Limiroli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Croce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marsida","family":"Teliti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedetto","family":"Cal\u00ec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tshering","family":"Dorji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Denegri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Magri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Rotondi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.etap.2025.104799_bib1","series-title":"PFOA, PFOS, and related PFAS chemicals","author":"American Cancer Society","year":"2025"},{"key":"10.1016\/j.etap.2025.104799_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2020.106204","article-title":"Hexafluoropropylene oxide-dimer acid (HFPO-DA or GenX) alters maternal and fetal glucose and lipid metabolism and produces neonatal mortality, low birthweight, and hepatomegaly in the Sprague-Dawley rat","volume":"146","author":"Conley","year":"2021","journal-title":"Environ. Int"},{"key":"10.1016\/j.etap.2025.104799_bib3","article-title":"Thyroid disrupting effects of old and new generation PFAS","volume":"11","author":"Coperchini","year":"2020","journal-title":"Front. Endocrinol."},{"key":"10.1016\/j.etap.2025.104799_bib4","doi-asserted-by":"crossref","first-page":"63522","DOI":"10.1007\/s11356-023-26797-6","article-title":"PFOA, PFHxA and C6O4 differently modulate the expression of CXCL8 in normal thyroid cells and in thyroid cancer cell lines","volume":"30","author":"Coperchini","year":"2023","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.etap.2025.104799_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2024.108717","article-title":"Do PFCAs drive the establishment of thyroid cancer microenvironment? Effects of C6O4, PFOA and PFHxA exposure in two models of human thyroid cells in primary culture","volume":"187","author":"Coperchini","year":"2024","journal-title":"Environ. Int."},{"key":"10.1016\/j.etap.2025.104799_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2024.125063","article-title":"In vitro study of the UV-filter homosalate effects on rat and human thyroid cells","volume":"363","author":"Coperchini","year":"2024","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.etap.2025.104799_bib7","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1080\/17446651.2024.2351885","article-title":"Per-polyfluoroalkyl substances (PFAS) as thyroid disruptors: is there evidence for multi-transgenerational effects?","volume":"19","author":"Coperchini","year":"2024","journal-title":"Expert Rev. Endocrinol. Metab."},{"key":"10.1016\/j.etap.2025.104799_bib8","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1007\/s40618-019-01062-1","article-title":"Effect of long- and short-chain perfluorinated compounds on cultured thyroid cells viability and response to TSH","volume":"42","author":"Croce","year":"2019","journal-title":"J. Endocrinol. Investig."},{"key":"10.1016\/j.etap.2025.104799_bib9","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.3892\/ijo.2016.3617","article-title":"Induction of TTF-1 or PAX-8 expression on proliferation and tumorigenicity in thyroid carcinomas","volume":"49","author":"Dupain","year":"2016","journal-title":"Int. J. Oncol."},{"key":"10.1016\/j.etap.2025.104799_bib10","unstructured":"European Chemical agency (ECHA), 2025. Sostanze per- e polifluoroalchiliche (PFAS). URL:\u3008https:\/\/echa.europa.eu\/it\/hot-topics\/perfluoroalkyl-chemicals-pfas\u3009."},{"key":"10.1016\/j.etap.2025.104799_bib11","series-title":"Health and environmental impacts prompt a call for strict ruling on ubiquitous \u2018forever chemicals\u2019. URL","author":"European Commission","year":"2023"},{"key":"10.1016\/j.etap.2025.104799_bib12","series-title":"Commission restricts use of a sub-group of PFAS chemicals to protect human health and the environment.","author":"European Commission","year":"2024"},{"key":"10.1016\/j.etap.2025.104799_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2023.115089","article-title":"Perfluorooctanoic acid-induced cell death via the dual roles of ROS-MAPK\/ERK signaling in ameloblast-lineage cells","volume":"260","author":"Fujiwara","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.etap.2025.104799_bib14","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/2082145","article-title":"The role of oxidative stress in physiopathology and pharmacological treatment with Pro- and antioxidant properties in chronic diseases","volume":"2020","author":"Garc\u00eda-S\u00e1nchez","year":"2020","journal-title":"Oxid. Med. Cell Longev."},{"key":"10.1016\/j.etap.2025.104799_bib15","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1055\/a-1057-9469","article-title":"Thyroid peroxidase revisited - what's new?","volume":"51","author":"Godlewska","year":"2019","journal-title":"Horm. Metab. Res."},{"key":"10.1016\/j.etap.2025.104799_bib16","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21082954","article-title":"ERGO: breaking down the wall between human health and environmental testing of endocrine disrupters","volume":"21","author":"Holbech","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.etap.2025.104799_bib17","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1002\/bit.10655","article-title":"Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types","volume":"83","author":"Kelm","year":"2003","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.etap.2025.104799_bib18","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.freeradbiomed.2022.09.024","article-title":"Deiodinases control local cellular and systemic thyroid hormone availability","volume":"193","author":"K\u00f6hrle","year":"2022","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.etap.2025.104799_bib19","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1152\/physrev.00010.2014","article-title":"Paradoxical roles of antioxidant enzymes: basic mechanisms and health implications","volume":"96","author":"Lei","year":"2016","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.etap.2025.104799_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2024.117098","article-title":"Perfluorooctanoic acid (PFOA) induces cardiotoxicity by activating the Keap1\/Nrf2 pathway in zebrafish (Danio rerio) embryos","volume":"285","author":"Liu","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.etap.2025.104799_bib21","doi-asserted-by":"crossref","first-page":"82","DOI":"10.3389\/fendo.2014.00082","article-title":"Thyroid hormone action: astrocyte-neuron communication","volume":"5","author":"Morte","year":"2014","journal-title":"Front. Endocrinol."},{"key":"10.1016\/j.etap.2025.104799_bib22","doi-asserted-by":"crossref","DOI":"10.3390\/ijms241411707","article-title":"Current review of increasing animal health threat of Per- and polyfluoroalkyl substances (PFAS): harms, limitations, and alternatives to manage their toxicity","volume":"24","author":"Peritore","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.etap.2025.104799_bib23","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1053\/j.semnuclmed.2023.11.002","article-title":"Autoimmune thyroid diseases","volume":"54","author":"Petranovi\u0107 Ov\u010dari\u010dek","year":"2024","journal-title":"Semin. Nucl. Med."},{"key":"10.1016\/j.etap.2025.104799_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.yexcr.2024.114017","article-title":"Truncated variants of thyroid hormone receptor beta display disease-inflicting malfunctioning at cellular level","volume":"437","author":"Rehman","year":"2024","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.etap.2025.104799_bib25","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1210\/jc.2012-2555","article-title":"Interferon-\u03b3 and tumor necrosis factor-\u03b1 sustain secretion of specific CXC chemokines in human thyrocytes: a first step toward a differentiation between autoimmune and tumor-related inflammation?","volume":"98","author":"Rotondi","year":"2013","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"10.1016\/j.etap.2025.104799_bib26","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.2174\/1389201024666221121160822","article-title":"Understanding the role of free radicals and antioxidant enzymes in human diseases","volume":"24","author":"Roy","year":"2023","journal-title":"Curr. Pharm. Biotechnol."},{"key":"10.1016\/j.etap.2025.104799_bib27","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.brainres.2007.02.042","article-title":"Inherited tertiary hypothyroidism in Sprague-Dawley rats","volume":"1148","author":"Stoica","year":"2007","journal-title":"Brain Res."},{"key":"10.1016\/j.etap.2025.104799_bib28","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1007\/s13197-022-05545-7","article-title":"Per- and polyfluoroalkyl substances (PFASs) in consumable species and food products","volume":"60","author":"Torres","year":"2023","journal-title":"J. Food Sci. Technol."},{"key":"10.1016\/j.etap.2025.104799_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2024.135771","article-title":"Thyroid cancer risk associated with perfluoroalkyl carboxylate exposure: assessment using a human dermal fibroblast-derived extracellular matrix-based thyroid cancer organoid","volume":"479","author":"Yoo","year":"2024","journal-title":"J. Hazard Mater."}],"container-title":["Environmental Toxicology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1382668925001747?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1382668925001747?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T12:26:22Z","timestamp":1772886382000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1382668925001747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":29,"alternative-id":["S1382668925001747"],"URL":"https:\/\/doi.org\/10.1016\/j.etap.2025.104799","relation":{},"ISSN":["1382-6689"],"issn-type":[{"value":"1382-6689","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation of the effects of PFCAs on spheroid thyroid cells in primary culture: Effects on thyroid function-related genes, tumorigenesis and oxidative stress","name":"articletitle","label":"Article Title"},{"value":"Environmental Toxicology and Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.etap.2025.104799","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"104799"}}