{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T03:15:43Z","timestamp":1768706143296,"version":"3.49.0"},"reference-count":35,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2012,1]]},"DOI":"10.1016\/j.exer.2011.11.015","type":"journal-article","created":{"date-parts":[[2011,12,8]],"date-time":"2011-12-08T18:55:11Z","timestamp":1323370511000},"page":"109-116","source":"Crossref","is-referenced-by-count":25,"title":["Effects of serum-free storage on morphology, phenotype, and viability of ex\u00a0vivo cultured human conjunctival epithelium"],"prefix":"10.1016","volume":"94","author":[{"given":"J.R.","family":"Eidet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.A.","family":"Utheim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Raeder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.A.","family":"Dartt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Lyberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Carreras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.T.","family":"Huynh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.B.","family":"Messelt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.E.","family":"Louch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Roald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.P.","family":"Utheim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.exer.2011.11.015_bib1","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.ajo.2004.10.056","article-title":"Autologous cultivated conjunctival transplantation for pterygium surgery","volume":"139","author":"Ang","year":"2005","journal-title":"American Journal of Ophthalmology"},{"key":"10.1016\/j.exer.2011.11.015_bib2","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1167\/iovs.09-3379","article-title":"Cultivated human conjunctival epithelial transplantation for total limbal stem cell deficiency","volume":"51","author":"Ang","year":"2010","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"10.1016\/j.exer.2011.11.015_bib3","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1038\/sj.eye.6702873","article-title":"Impression cytology with scanning electron microscopy: a new method in the study of conjunctival microvilli","volume":"22","author":"Cennamo","year":"2008","journal-title":"Eye (London)"},{"key":"10.1016\/j.exer.2011.11.015_bib4","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.exer.2003.10.005","article-title":"Control of mucin production by ocular surface epithelial cells","volume":"78","author":"Dartt","year":"2004","journal-title":"Experimental Eye Research"},{"key":"10.1016\/j.exer.2011.11.015_bib5","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1034\/j.1600-0420.1998.760311.x","article-title":"Expression of proliferating cell nuclear antigen in corneas kept in long term culture","volume":"76","author":"Gan","year":"1998","journal-title":"Acta Ophthalmologica Scandinavica"},{"key":"10.1016\/j.exer.2011.11.015_bib6","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1089\/ten.tec.2009.0277","article-title":"Validation system of tissue engineered epithelial cell sheets for corneal regenerative medicine","volume":"16","author":"Hayashi","year":"2010","journal-title":"Tissue Engineering Part C Methods"},{"key":"10.1016\/j.exer.2011.11.015_bib7","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/j.1463-5224.2009.00671.x","article-title":"Effect of bovine freeze-dried amniotic membrane (Amnisite-BA) on uncomplicated canine corneal erosion","volume":"12","author":"Kim","year":"2009","journal-title":"Veterinary Ophthalmology"},{"key":"10.1016\/j.exer.2011.11.015_bib8","first-page":"1069","article-title":"A\u00a0partial-thickness epithelial defect increases the adherence of Pseudomonas aeruginosa to the cornea","volume":"30","author":"Klotz","year":"1989","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"10.1016\/j.exer.2011.11.015_bib9","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s00417-005-0095-3","article-title":"Comparison of intact and denuded amniotic membrane as a substrate for cell-suspension culture of human limbal epithelial cells","volume":"245","author":"Koizumi","year":"2007","journal-title":"Graefes Archive for Clinical and Experimental Ophthalmology"},{"key":"10.1016\/j.exer.2011.11.015_bib10","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1083\/jcb.139.3.749","article-title":"ERM (ezrin\/radixin\/moesin)-based molecular mechanism of microvillar breakdown at an early stage of apoptosis","volume":"139","author":"Kondo","year":"1997","journal-title":"Journal of Cell Biology"},{"key":"10.1016\/j.exer.2011.11.015_bib11","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1167\/iovs.05-0804","article-title":"Conjunctival surface changes in patients with Sjogren\u2019s syndrome: a transmission electron microscopy study","volume":"47","author":"Koufakis","year":"2006","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"10.1016\/j.exer.2011.11.015_bib12","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/S0002-9394(14)70125-4","article-title":"Amniotic membrane transplantation for persistent epithelial defects with ulceration","volume":"123","author":"Lee","year":"1997","journal-title":"American Journal of Ophthalmology"},{"key":"10.1016\/j.exer.2011.11.015_bib13","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1006\/exer.2001.1163","article-title":"Expansion of conjunctival epithelial progenitor cells on amniotic membrane","volume":"74","author":"Meller","year":"2002","journal-title":"Experimental Eye Research"},{"key":"10.1016\/j.exer.2011.11.015_bib14","first-page":"243","article-title":"Amniotic membrane transplantation in the human eye","volume":"108","author":"Meller","year":"2011","journal-title":"Deutsches \u00c4rzteblatt International"},{"key":"10.1016\/j.exer.2011.11.015_bib15","first-page":"878","article-title":"Conjunctival epithelial cell differentiation on amniotic membrane","volume":"40","author":"Meller","year":"1999","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"10.1016\/j.exer.2011.11.015_bib16","first-page":"1138","article-title":"Autologous transplantation of conjunctival epithelial cells cultured on amniotic membrane in a rabbit model","volume":"13","author":"Ono","year":"2007","journal-title":"Molecular Vision"},{"key":"10.1016\/j.exer.2011.11.015_bib17","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1083\/jcb.145.4.769","article-title":"Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface","volume":"145","author":"Pellegrini","year":"1999","journal-title":"Journal of Cell Biology"},{"key":"10.1016\/j.exer.2011.11.015_bib18","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0161-6420(97)30330-3","article-title":"Correlation of goblet cell density and mucosal epithelial membrane mucin expression with rose bengal staining in patients with ocular irritation","volume":"104","author":"Pflugfelder","year":"1997","journal-title":"Ophthalmology"},{"key":"10.1016\/j.exer.2011.11.015_bib19","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1097\/ICO.0b013e3181ba0c94","article-title":"The impact of de-epithelialization of the amniotic membrane matrix on morphology of cultured human limbal epithelial cells subject to eye bank storage","volume":"29","author":"Raeder","year":"2010","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2011.11.015_bib20","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/j.1600-0420.2006.00873.x","article-title":"Effect of limbal explant orientation on the histology, phenotype, ultrastructure and barrier function of cultured limbal epithelial cells","volume":"85","author":"Raeder","year":"2007","journal-title":"Acta Ophthalmologica Scandinavica"},{"key":"10.1016\/j.exer.2011.11.015_bib21","first-page":"5717","article-title":"Eye bank storage of cultured corneal epithelium: sterility testing of storage media, long-term preservation, and deepithelialization of the amniotic membrane substrate","volume":"49","author":"Raeder","year":"2008","journal-title":"ARVO Meeting Abstracts"},{"key":"10.1016\/j.exer.2011.11.015_bib22","doi-asserted-by":"crossref","first-page":"5484","DOI":"10.1167\/iovs.07-0494","article-title":"Effects of organ culture and optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium","volume":"48","author":"Raeder","year":"2007","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"10.1016\/j.exer.2011.11.015_bib23","doi-asserted-by":"crossref","first-page":"913","DOI":"10.3109\/02713680903198045","article-title":"Tissue engineering for conjunctival reconstruction: established methods and future outlooks","volume":"34","author":"Schrader","year":"2009","journal-title":"Current Eye Research"},{"key":"10.1016\/j.exer.2011.11.015_bib24","doi-asserted-by":"crossref","first-page":"3820","DOI":"10.1167\/iovs.06-0293","article-title":"Establishment of a cultivated human conjunctival epithelium as an alternative tissue source for autologous corneal epithelial transplantation","volume":"47","author":"Tanioka","year":"2006","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"10.1016\/j.exer.2011.11.015_bib25","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1002\/jbm.a.31304","article-title":"Quantitative analysis of cell adhesion on aligned micro- and nanofibers","volume":"84","author":"Tian","year":"2008","journal-title":"Journal of Biomedical Materials Research A"},{"key":"10.1016\/j.exer.2011.11.015_bib26","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1023\/A:1017995810755","article-title":"Amniotic membrane transplantation for ocular surface reconstruction","volume":"21","author":"Tseng","year":"2001","journal-title":"Bioscience Reports"},{"key":"10.1016\/j.exer.2011.11.015_bib27","first-page":"1778","article-title":"Storage of cultured human limbal epithelial cells in Optisol-GS at 23\u00b0C versus 5\u00b0C","volume":"50","author":"Utheim","year":"2009","journal-title":"ARVO Meeting Abstracts"},{"key":"10.1016\/j.exer.2011.11.015_bib28","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1136\/bjo.2006.103218","article-title":"A\u00a0novel method for preserving cultured limbal epithelial cells","volume":"91","author":"Utheim","year":"2007","journal-title":"British Journal of Ophthalmology"},{"key":"10.1016\/j.exer.2011.11.015_bib29","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1136\/bjo.2008.149591","article-title":"Sterility control and long-term eye-bank storage of cultured human limbal epithelial cells for transplantation","volume":"93","author":"Utheim","year":"2009","journal-title":"British Journal of Ophthalmology"},{"key":"10.1016\/j.exer.2011.11.015_bib30","article-title":"Comparison of storage of cultured human limbal epithelial cells in HEPES-MEM, optisol-GS and PAA quantum 286 for 4 days at 23\u00b0C","author":"Utheim","year":"2011","journal-title":"ARVO Meeting Abstracts"},{"key":"10.1016\/j.exer.2011.11.015_bib31","series-title":"European Eye Bank Association, Annual Meeting, Amsterdam, The Netherlands","article-title":"Effects of the size of explants on epithelial thickness, number of cell layers, ultrastructure, and phenotype of cultured limbal epithelial cells","author":"Utheim","year":"2009"},{"key":"10.1016\/j.exer.2011.11.015_bib32","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1001\/archopht.1975.01010020285007","article-title":"Ultrastructure of human organ-cultured cornea. II. Stroma and epithelium","volume":"93","author":"Van Horn","year":"1975","journal-title":"Archives of Ophthalmology"},{"key":"10.1016\/j.exer.2011.11.015_bib33","first-page":"753","article-title":"Clonal analysis of the in\u00a0vivo differentiation potential of keratinocytes","volume":"38","author":"Wei","year":"1997","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"10.1016\/j.exer.2011.11.015_bib34","first-page":"1814","article-title":"In\u00a0vitro growth and differentiation of\u00a0rabbit bulbar, fornix, and palpebral conjunctival epithelia. Implications on conjunctival epithelial transdifferentiation and stem cells","volume":"34","author":"Wei","year":"1993","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"10.1016\/j.exer.2011.11.015_bib35","first-page":"3138","article-title":"Mucocutaneous junction as the major source of replacement palpebral conjunctival epithelial cells","volume":"40","author":"Wirtschafter","year":"1999","journal-title":"Investigative Ophthalmology and Visual Science"}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483511003903?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483511003903?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T02:57:36Z","timestamp":1543373856000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483511003903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["S0014483511003903"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2011.11.015","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2012,1]]}}}