{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:42:39Z","timestamp":1758127359383},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,7,10]],"date-time":"2018-07-10T00:00:00Z","timestamp":1531180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"NIH\/NEI","award":["EY023364"],"award-info":[{"award-number":["EY023364"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2018,11]]},"DOI":"10.1016\/j.exer.2018.07.011","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T00:22:15Z","timestamp":1531268535000},"page":"188-195","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Establishment of a conditionally immortalized mouse optic nerve astrocyte line"],"prefix":"10.1016","volume":"176","author":[{"given":"Yang","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaurang C.","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiming","family":"Mao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abbot F.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.exer.2018.07.011_bib1","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1016\/S0002-9440(10)65376-X","article-title":"Physiological and cytogenetic characterization of immortalized human endometriotic cells containing episomal simian virus 40 DNA","volume":"154","author":"Akoum","year":"1999","journal-title":"Am. J. Pathol."},{"issue":"11","key":"10.1016\/j.exer.2018.07.011_bib2","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1046\/j.1440-1746.2000.2328.x","article-title":"Conditionally immortalized mouse hepatocytes for use in liver gene therapy","volume":"15","author":"Allen","year":"2000","journal-title":"J. Gastroenterol. Hepatol."},{"key":"10.1016\/j.exer.2018.07.011_bib3","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s13039-015-0183-y","article-title":"Karyotype alteration generates the neoplastic phenotypes of SV40-infected human and rodent cells","volume":"8","author":"Bloomfield","year":"2015","journal-title":"Mol. Cytogenet."},{"issue":"2","key":"10.1016\/j.exer.2018.07.011_bib4","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/0014-4827(86)90181-3","article-title":"Transformation of human cultured fibroblasts with plasmids carrying dominant selection markers and immortalizing potential","volume":"167","author":"Chang","year":"1986","journal-title":"Exp. Cell Res."},{"issue":"7480","key":"10.1016\/j.exer.2018.07.011_bib5","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1038\/nature12776","article-title":"Astrocytes mediate synapse elimination through MEGF10 and MERTK pathways","volume":"504","author":"Chung","year":"2013","journal-title":"Nature"},{"issue":"4","key":"10.1016\/j.exer.2018.07.011_bib6","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1042\/bst0300800","article-title":"Conditionally immortalized cell lines as model systems for high-throughput biology in drug discovery","volume":"30","author":"Daniele","year":"2002","journal-title":"Biochem. Soc. Trans."},{"issue":"11","key":"10.1016\/j.exer.2018.07.011_bib7","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1038\/nprot.2008.171","article-title":"A rapid Percoll gradient procedure for preparation of synaptosomes","volume":"3","author":"Dunkley","year":"2008","journal-title":"Nat. Protoc."},{"issue":"2","key":"10.1016\/j.exer.2018.07.011_bib8","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0042-6822(85)90130-8","article-title":"P53-transformation-related protein: kinetics of synthesis and accumulation in SV40-infected primary mouse kidney cell cultures","volume":"147","author":"Duthu","year":"1985","journal-title":"Virology"},{"issue":"5","key":"10.1016\/j.exer.2018.07.011_bib9","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.neuron.2011.07.022","article-title":"Development of a method for the purification and culture of rodent astrocytes","volume":"71","author":"Foo","year":"2011","journal-title":"Neuron"},{"issue":"5","key":"10.1016\/j.exer.2018.07.011_bib10","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1083\/jcb.107.5.1977","article-title":"Antigenic and functional characterization of a rat central nervous system-derived cell line immortalized by a retroviral vector","volume":"107","author":"Geller","year":"1988","journal-title":"J. Cell Biol."},{"issue":"1","key":"10.1016\/j.exer.2018.07.011_bib11","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1006\/dbio.1993.1223","article-title":"The characterization of astrocyte cell lines that display properties of glial scar tissue","volume":"159","author":"Groves","year":"1993","journal-title":"Dev. Biol."},{"issue":"2","key":"10.1016\/j.exer.2018.07.011_bib12","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s11357-999-0008-1","article-title":"Mechanism of immortalization","volume":"22","author":"Hubbard","year":"1999","journal-title":"Age (Omaha)"},{"issue":"5","key":"10.1016\/j.exer.2018.07.011_bib13","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1128\/MCB.12.5.2273","article-title":"Altered chromosome 6 in immortal human fibroblasts","volume":"12","author":"Hubbard-Smith","year":"1992","journal-title":"Mol. Cell Biol."},{"issue":"12","key":"10.1016\/j.exer.2018.07.011_bib14","doi-asserted-by":"crossref","first-page":"5096","DOI":"10.1073\/pnas.88.12.5096","article-title":"Direct derivation of conditionally immortal cell lines from an H-2Kb-tsA58 transgenic mouse","volume":"88","author":"Jat","year":"1991","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"3 Pt 2","key":"10.1016\/j.exer.2018.07.011_bib15","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1523\/JNEUROSCI.14-03-01441.1994","article-title":"Optic nerve injury alters basic fibroblast growth factor localization in the retina and optic tract","volume":"14","author":"Kostyk","year":"1994","journal-title":"J. Neurosci."},{"issue":"10","key":"10.1016\/j.exer.2018.07.011_bib16","first-page":"2315","article-title":"Neurotrophin and neurotrophin receptor expression by cells of the human lamina cribrosa","volume":"42","author":"Lambert","year":"2001","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"issue":"12","key":"10.1016\/j.exer.2018.07.011_bib17","doi-asserted-by":"crossref","first-page":"6447","DOI":"10.1128\/JVI.65.12.6447-6453.1991","article-title":"The ability of large T antigen to complex with p53 is necessary for the increased life span and partial transformation of human cells by simian virus 40","volume":"65","author":"Lin","year":"1991","journal-title":"J. Virol."},{"issue":"33","key":"10.1016\/j.exer.2018.07.011_bib18","doi-asserted-by":"crossref","first-page":"10509","DOI":"10.1073\/pnas.1506486112","article-title":"Astrocytes phagocytose focal dystrophies from shortening myelin segments in the optic nerve of Xenopus laevis at metamorphosis","volume":"112","author":"Mills","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"3","key":"10.1016\/j.exer.2018.07.011_bib19","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1073\/pnas.1013965108","article-title":"Myelination transition zone astrocytes are constitutively phagocytic and have synuclein dependent reactivity in glaucoma","volume":"108","author":"Nguyen","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"54","key":"10.1016\/j.exer.2018.07.011_bib20","doi-asserted-by":"crossref","first-page":"7899","DOI":"10.1038\/sj.onc.1204936","article-title":"Transforming functions of simian virus 40","volume":"20","author":"Saenz-Robles","year":"2001","journal-title":"Oncogene"},{"issue":"1","key":"10.1016\/j.exer.2018.07.011_bib21","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0042-6822(91)90008-Y","article-title":"Expression of SV40 large T antigen, but not small t antigen, is required for the induction of chromosomal aberrations in transformed human cells","volume":"180","author":"Stewart","year":"1991","journal-title":"Virology"},{"issue":"7","key":"10.1016\/j.exer.2018.07.011_bib22","first-page":"1392","article-title":"Development of characterization of a immortal and differentiated murine trabecular meshwork cell line","volume":"40","author":"Tamm","year":"1999","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"issue":"3","key":"10.1016\/j.exer.2018.07.011_bib23","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1177\/096368979700600306","article-title":"Growth properties of neural cell lines immortalized with the tsA58 allele of SV40 large T antigen","volume":"6","author":"Truckenmiller","year":"1997","journal-title":"Cell Transplant."},{"issue":"8","key":"10.1016\/j.exer.2018.07.011_bib24","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/nrn1722","article-title":"Astrocytes, from brain glue to communication elements: the revolution continues","volume":"6","author":"Volterra","year":"2005","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.exer.2018.07.011_bib25","article-title":"Preparation of synaptoneurosomes from mouse cortex using a discontinuous percoll-sucrose density gradient","volume":"55","author":"Westmark","year":"2011","journal-title":"JoVE"},{"issue":"2","key":"10.1016\/j.exer.2018.07.011_bib26","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1073\/pnas.90.2.587","article-title":"Establishment of conditionally immortalized epithelial cell lines from both colon and small intestine of adult H-2Kb-tsA58 transgenic mice","volume":"90","author":"Whitehead","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U. S. A."}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483518301611?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483518301611?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T02:30:08Z","timestamp":1720405808000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483518301611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11]]},"references-count":26,"alternative-id":["S0014483518301611"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2018.07.011","relation":{},"ISSN":["0014-4835"],"issn-type":[{"type":"print","value":"0014-4835"}],"subject":[],"published":{"date-parts":[[2018,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Establishment of a conditionally immortalized mouse optic nerve astrocyte line","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2018.07.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}