{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:51:13Z","timestamp":1790481073180,"version":"4.1.0"},"reference-count":181,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2020,10]]},"DOI":"10.1016\/j.exer.2020.108181","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T02:16:22Z","timestamp":1597198582000},"page":"108181","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["Niche regulation of limbal epithelial stem cells: HC-HA\/PTX3 as surrogate matrix niche"],"prefix":"10.1016","volume":"199","author":[{"given":"Scheffer C.G.","family":"Tseng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Szu-Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0788-4374","authenticated-orcid":false,"given":"Olivia G.","family":"Mead","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Tighe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exer.2020.108181_bib1","doi-asserted-by":"crossref","first-page":"2737","DOI":"10.1089\/ten.tea.2013.0661","article-title":"Matrix composition and mechanics direct proangiogenic signaling from mesenchymal stem cells","volume":"20","author":"Abdeen","year":"2014","journal-title":"Tissue Eng."},{"key":"10.1016\/j.exer.2020.108181_bib2","doi-asserted-by":"crossref","first-page":"110","DOI":"10.5966\/sctm.2011-0037","article-title":"Concise review: limbal stem cell deficiency, dysfunction, and distress","volume":"1","author":"Ahmad","year":"2012","journal-title":"Stem Cells Transl Med"},{"key":"10.1016\/j.exer.2020.108181_bib3","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s11701-019-00972-9","article-title":"A single-center, retrospective review of robot-assisted laparoscopic prostatectomy with and 1 without cryopreserved umbilical cord allograft in improving continence recovery","volume":"2","author":"Ahmed","year":"2020","journal-title":"Journal of robotic surgery"},{"key":"10.1016\/j.exer.2020.108181_bib4","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.ajo.2014.06.002","article-title":"Modified simple limbal epithelial transplantation using cryopreserved amniotic membrane for unilateral limbal stem cell deficiency","volume":"158","author":"Amescua","year":"2014","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib5","doi-asserted-by":"crossref","first-page":"13291","DOI":"10.1073\/pnas.0405250101","article-title":"Nascent blood vessels in the skin arise from nestin-expressing hair-follicle cells","volume":"101","author":"Amoh","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.exer.2020.108181_bib6","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1136\/bjo.85.5.567","article-title":"Amniotic membrane transplantation for partial limbal stem cell deficiency","volume":"85","author":"Anderson","year":"2001","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib7","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1167\/iovs.03-1361","article-title":"Development of a conjunctival epithelial equivalent with improved proliferative properties using a multistep serum-free culture system","volume":"45","author":"Ang","year":"2004","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib8","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1083\/jcb.200703003","article-title":"C\/EBPdelta regulates cell cycle and self-renewal of human limbal stem cells","volume":"177","author":"Barbaro","year":"2007","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.exer.2020.108181_bib9","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3009644","article-title":"Human limbal biopsy-derived stromal stem cells prevent corneal scarring","volume":"6","author":"Basu","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.exer.2020.108181_bib10","doi-asserted-by":"crossref","DOI":"10.1371\/annotation\/5326b2ea-4388-4d50-8b86-407a3c5250e4","article-title":"Transcriptional dissection of human limbal niche compartments by massive parallel sequencing","volume":"8","author":"Bath","year":"2013","journal-title":"PloS One"},{"key":"10.1016\/j.exer.2020.108181_bib11","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0925-4773(02)00260-5","article-title":"Pax6 heterozygous eyes show defects in chamber angle differentiation that are associated with a wide spectrum of other anterior eye segment abnormalities","volume":"118","author":"Baulmann","year":"2002","journal-title":"Mech. Dev."},{"key":"10.1016\/j.exer.2020.108181_bib12","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1074\/jbc.274.1.298","article-title":"Structural characterization of inter-alpha-inhibitor. Evidence for an extended shape","volume":"274","author":"Blom","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exer.2020.108181_bib13","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1002\/sctm.19-0021","article-title":"Long-term results of cultured limbal stem cell versus limbal tissue transplantation in stage III limbal deficiency","volume":"8","author":"Borderie","year":"2019","journal-title":"Stem Cells Transl Med"},{"key":"10.1016\/j.exer.2020.108181_bib14","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s10856-012-4774-4","article-title":"In vitro characterization and ex vivo surgical evaluation of human hair keratin films in ocular surface reconstruction after sterilization processing","volume":"24","author":"Borrelli","year":"2013","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"10.1016\/j.exer.2020.108181_bib15","doi-asserted-by":"crossref","first-page":"5109","DOI":"10.1167\/iovs.11-8673","article-title":"Mesenchymal stem cells in the human corneal limbal stroma","volume":"53","author":"Branch","year":"2012","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib16","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.jcyt.2013.07.006","article-title":"Immunosuppressive properties of mesenchymal stromal cell cultures derived from the limbus of human and rabbit corneas","volume":"16","author":"Bray","year":"2014","journal-title":"Cytotherapy"},{"key":"10.1016\/j.exer.2020.108181_bib17","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1111\/wrr.12456","article-title":"A retrospective study of cryopreserved umbilical cord as an adjunctive therapy to promote the healing of chronic, complex foot ulcers with underlying osteomyelitis","volume":"24","author":"Caputo","year":"2016","journal-title":"Wound Repair Regen."},{"key":"10.1016\/j.exer.2020.108181_bib18","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1242\/dev.107631","article-title":"Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium","volume":"141","author":"Castillo","year":"2014","journal-title":"Development"},{"key":"10.1016\/j.exer.2020.108181_bib19","first-page":"1301","article-title":"Corneal epithelial wound healing in partial limbal deficiency","volume":"31","author":"Chen","year":"1990","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib20","first-page":"2219","article-title":"Abnormal corneal epithelial wound healing in partial-thickness removal of limbal epithelium","volume":"32","author":"Chen","year":"1991","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib21","doi-asserted-by":"crossref","first-page":"9763","DOI":"10.1038\/s41598-019-45100-7","article-title":"Pax 6 controls neural crest potential of limbal niche cells to support self-renewal of limbal epithelial stem cells","volume":"9","author":"Chen","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.exer.2020.108181_bib22","doi-asserted-by":"crossref","first-page":"3341","DOI":"10.1002\/stem.2091","article-title":"HC-HA\/PTX3 purified from amniotic membrane promotes BMP signaling in limbal niche cells to maintain quiescence of limbal epithelial progenitor\/stem cells","volume":"11","author":"Chen","year":"2015","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib23","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1089\/ten.tec.2010.0609","article-title":"A new isolation method of human limbal progenitor cells by maintaining close association with their niche cells","volume":"17","author":"Chen","year":"2011","journal-title":"Tissue Eng. C Methods"},{"key":"10.1016\/j.exer.2020.108181_bib24","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.1167\/iovs.14-14441","article-title":"Isolation and characterization of mesenchymal progenitor cells from human orbital adipose tissue","volume":"55","author":"Chen","year":"2014","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib25","article-title":"Adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation","volume":"9","author":"Chen","year":"2014","journal-title":"PloS One"},{"key":"10.1016\/j.exer.2020.108181_bib26","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1634\/stemcells.2006-0677","article-title":"Human amniotic epithelial cells as novel feeder layers for promoting ex vivo expansion of limbal epithelial progenitor cells","volume":"25","author":"Chen","year":"2007","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib27","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1167\/tvst.5.3.9","article-title":"Morselized amniotic membrane tissue for refractory corneal epithelial defects in cicatricial ocular surface diseases","volume":"5","author":"Cheng","year":"2016","journal-title":"Translational vision science & technology"},{"key":"10.1016\/j.exer.2020.108181_bib28","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1159\/000486922","article-title":"Ocular injury due to potassium permanganate granules","volume":"9","author":"Chirapapaisan","year":"2018","journal-title":"Case Rep Ophthalmol"},{"key":"10.1016\/j.exer.2020.108181_bib29","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1167\/iovs.03-1118","article-title":"Corneal development, limbal stem cell function, and corneal epithelial cell migration in the Pax6(+\/-) mouse","volume":"45","author":"Collinson","year":"2004","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib30","doi-asserted-by":"crossref","first-page":"465","DOI":"10.12968\/jowc.2014.23.10.465","article-title":"Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated amniotic membrane\/chorion tissue","volume":"23","author":"Cooke","year":"2014","journal-title":"J. Wound Care"},{"key":"10.1016\/j.exer.2020.108181_bib31","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0092-8674(89)90958-6","article-title":"Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells","volume":"57","author":"Cotsarelis","year":"1989","journal-title":"Cell"},{"key":"10.1016\/j.exer.2020.108181_bib32","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.exer.2016.04.017","article-title":"PAX6: 25th anniversary and more to learn","volume":"156","author":"Cvekl","year":"2017","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2020.108181_bib33","first-page":"257","article-title":"Safety analysis of cryopreserved amniotic membrane\/umbilical cord tissue in foot and ankle surgery: a consecutive case series of 124 patients","volume":"25","author":"DeMill","year":"2014","journal-title":"Surg. Technol. Int."},{"key":"10.1016\/j.exer.2020.108181_bib34","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1097\/ICO.0000000000001820","article-title":"Global consensus on definition, classification, diagnosis, and staging of limbal stem cell deficiency","volume":"38","author":"Deng","year":"2019","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2020.108181_bib35","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/stem.1769","article-title":"Tracing the fate of limbal epithelial progenitor cells in the murine cornea","volume":"33","author":"Di Girolamo","year":"2015","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib36","doi-asserted-by":"crossref","first-page":"9523","DOI":"10.1073\/pnas.0503437102","article-title":"Isoforms of DeltaNp63 and the migration of ocular limbal cells in human corneal regeneration","volume":"102","author":"Di Iorio","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.exer.2020.108181_bib37","series-title":"Amniotic Membrane for Acute Stevens-Johnson Syndrome, EyeNet","author":"Doran","year":"2008"},{"key":"10.1016\/j.exer.2020.108181_bib38","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.devbrainres.2005.09.008","article-title":"The transdifferentiation potential of limbal fibroblast-like cells","volume":"160","author":"Dravida","year":"2005","journal-title":"Brain Res.Dev.Brain Res."},{"key":"10.1016\/j.exer.2020.108181_bib39","doi-asserted-by":"crossref","first-page":"2815","DOI":"10.1242\/dev.038620","article-title":"Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis","volume":"136","author":"Driskell","year":"2009","journal-title":"Development"},{"key":"10.1016\/j.exer.2020.108181_bib40","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1634\/stemcells.2004-0256","article-title":"Multipotent stem cells in human corneal stroma","volume":"23","author":"Du","year":"2005","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib41","doi-asserted-by":"crossref","first-page":"5038","DOI":"10.1167\/iovs.07-0587","article-title":"Secretion and organization of a cornea-like tissue in vitro by stem cells from human corneal stroma","volume":"48","author":"Du","year":"2007","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib42","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1136\/bjo.85.11.1379","article-title":"A new classification of ocular surface burns","volume":"85","author":"Dua","year":"2001","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib43","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1136\/bjo.2004.049742","article-title":"Limbal epithelial crypts: a novel anatomical structure and a putative limbal stem cell niche","volume":"89","author":"Dua","year":"2005","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib44","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.exer.2015.06.026","article-title":"Limbal melanocytes support limbal epithelial stem cells in 2D and 3D microenvironments","volume":"138","author":"Dziasko","year":"2015","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2020.108181_bib45","first-page":"1450","article-title":"Comparison of central and peripheral human corneal epithelium in tissue culture","volume":"28","author":"Ebato","year":"1987","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib46","doi-asserted-by":"crossref","first-page":"8138","DOI":"10.1167\/iovs.11-8140","article-title":"Vitronectin: a matrix support factor for human limbal epithelial progenitor cells","volume":"52","author":"Echevarria","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib47","doi-asserted-by":"crossref","first-page":"15975","DOI":"10.1016\/S0021-9258(18)71575-5","article-title":"Analysis of inter-alpha-trypsin inhibitor and a novel trypsin inhibitor, pre-alpha-trypsin inhibitor, from human plasma. Polypeptide chain stoichiometry and assembly by glycan","volume":"264","author":"Enghild","year":"1989","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exer.2020.108181_bib48","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.cell.2006.06.044","article-title":"Matrix elasticity directs stem cell lineage specification","volume":"126","author":"Engler","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.exer.2020.108181_bib49","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1097\/01.ico.0000151501.80952.c5","article-title":"Amniotic membrane transplantation for ocular surface reconstruction","volume":"24","author":"Fernandes","year":"2005","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2020.108181_bib50","doi-asserted-by":"crossref","DOI":"10.1136\/bmjophth-2018-000164","article-title":"Twenty years of limbal epithelial therapy: an update on managing limbal stem cell deficiency","volume":"3","author":"Fernandez-Buenaga","year":"2018","journal-title":"BMJ open ophthalmology"},{"key":"10.1016\/j.exer.2020.108181_bib51","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.12968\/jowc.2019.28.Sup2.S4","article-title":"Cryopreserved amniotic membrane and umbilical cord for a radiation-induced wound with exposed dura: a case report","volume":"28","author":"Fernandez","year":"2019","journal-title":"J. Wound Care"},{"key":"10.1016\/j.exer.2020.108181_bib52","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.exer.2014.06.020","article-title":"Differential nuclear expression of Yap in basal epithelial cells across the cornea and substrates of differing stiffness","volume":"127","author":"Foster","year":"2014","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2020.108181_bib53","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/S0092-8674(04)00255-7","article-title":"Socializing with the neighbors: stem cells and their niche","volume":"116","author":"Fuchs","year":"2004","journal-title":"Cell"},{"key":"10.1016\/j.exer.2020.108181_bib54","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1242\/dev.00422","article-title":"Impaired cumulus mucification and female sterility in tumor necrosis factor-induced protein-6 deficient mice","volume":"130","author":"Fulop","year":"2003","journal-title":"Development"},{"key":"10.1016\/j.exer.2020.108181_bib55","doi-asserted-by":"crossref","first-page":"5795","DOI":"10.1167\/iovs.14-14664","article-title":"Comparative transcriptomic analysis of cultivated limbal epithelium and donor corneal tissue reveals altered wound healing gene expression","volume":"55","author":"Gallagher","year":"2014","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib56","doi-asserted-by":"crossref","first-page":"4407","DOI":"10.1167\/iovs.17-22326","article-title":"Hyaluronan rich microenvironment in the limbal stem cell niche regulates limbal stem cell differentiation","volume":"58","author":"Gesteira","year":"2017","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib57","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1007\/s10616-015-9938-x","article-title":"Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery","volume":"68","author":"Gho","year":"2016","journal-title":"Cytotechnology"},{"key":"10.1016\/j.exer.2020.108181_bib58","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0188398","article-title":"Easy xeno-free and feeder-free method for isolating and growing limbal stromal and epithelial stem cells of the human cornea","volume":"12","author":"Ghoubay-Benallaoua","year":"2017","journal-title":"PloS One"},{"key":"10.1016\/j.exer.2020.108181_bib59","first-page":"155","article-title":"Limbal palisades of Vogt","volume":"80","author":"Goldberg","year":"1982","journal-title":"Trans. Am. Ophthalmol. Soc."},{"key":"10.1016\/j.exer.2020.108181_bib60","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/S0161-6420(02)01888-2","article-title":"Amniotic membrane transplantation for partial and total limbal stem cell deficiency secondary to chemical burn","volume":"110","author":"Gomes","year":"2003","journal-title":"Ophthalmology"},{"key":"10.1016\/j.exer.2020.108181_bib61","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1038\/s41467-019-09331-6","article-title":"Assessment of corneal substrate biomechanics and its effect on epithelial stem cell maintenance and differentiation","volume":"10","author":"Gouveia","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.exer.2020.108181_bib62","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1242\/dev.041103","article-title":"Compartmentalized organization: a common and required feature of stem cell niches?","volume":"137","author":"Greco","year":"2010","journal-title":"Development"},{"key":"10.1016\/j.exer.2020.108181_bib63","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.exer.2015.08.003","article-title":"Three-dimensional structure of the mammalian limbal stem cell niche","volume":"140","author":"Grieve","year":"2015","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2020.108181_bib64","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.scr.2014.01.003","article-title":"Integration of BMP\/Wnt signaling to control clonal growth of limbal epithelial progenitor cells by niche cells","volume":"12","author":"Han","year":"2014","journal-title":"Stem Cell Res."},{"key":"10.1016\/j.exer.2020.108181_bib65","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1097\/IIO.0b013e3181924e54","article-title":"The structure and function of the limbal stem cell and the disease states associated with limbal stem cell deficiency","volume":"49","author":"Hatch","year":"2009","journal-title":"Int. Ophthalmol. Clin."},{"key":"10.1016\/j.exer.2020.108181_bib66","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1634\/stemcells.2006-0167","article-title":"N-Cadherin is expressed by putative stem\/progenitor cells and melanocytes in the human limbal epithelial stem cell niche","volume":"25","author":"Hayashi","year":"2007","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib67","doi-asserted-by":"crossref","first-page":"4468","DOI":"10.1167\/iovs.08-1781","article-title":"Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract","volume":"49","author":"He","year":"2008","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib68","doi-asserted-by":"crossref","first-page":"20136","DOI":"10.1074\/jbc.M109.021881","article-title":"Biochemical characterization and function of complexes formed by hyaluronan and the heavy chains of inter-alpha-inhibitor (HC*HA) purified from extracts of human amniotic membrane","volume":"284","author":"He","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exer.2020.108181_bib69","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1167\/iovs.13-13094","article-title":"In vivo downregulation of innate and adaptive immune responses in corneal allograft rejection by HC-HA\/PTX3 complex purified from amniotic membrane","volume":"55","author":"He","year":"2014","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib70","doi-asserted-by":"crossref","first-page":"25792","DOI":"10.1074\/jbc.M113.479584","article-title":"Immobilized heavy chain-hyaluronic acid polarizes lipopolysaccharide-activated macrophages toward M2 phenotype","volume":"288","author":"He","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exer.2020.108181_bib71","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1002\/jcp.24160","article-title":"Transcriptional regulators of the DeltaNp63: their role in limbal epithelial cell proliferation","volume":"228","author":"Hsueh","year":"2013","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.exer.2020.108181_bib72","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1159\/000086115","article-title":"Limbal graft and\/or amniotic membrane transplantation in the treatment of ocular burns","volume":"219","author":"Ivekovic","year":"2005","journal-title":"Ophthalmologica"},{"key":"10.1016\/j.exer.2020.108181_bib73","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1002\/sctm.19-0203","article-title":"Simple limbal epithelial transplantation: current status and future perspectives","volume":"9","author":"Jackson","year":"2020","journal-title":"Stem Cells Transl Med"},{"key":"10.1016\/j.exer.2020.108181_bib74","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1530\/rep.0.1250027","article-title":"Role of tumour necrosis factor stimulated gene 6 (TSG-6) in the coupling of inter-alpha-trypsin inhibitor to hyaluronan in human follicular fluid","volume":"125","author":"Jessen","year":"2003","journal-title":"Reproduction"},{"key":"10.1016\/j.exer.2020.108181_bib75","article-title":"Corneal nerve regeneration after self-retained cryopreserved amniotic membrane in dry eye disease","volume":"2017:6404918","author":"John","year":"2017","journal-title":"Journal of Ophthalmology"},{"key":"10.1016\/j.exer.2020.108181_bib76","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.scr.2012.01.001","article-title":"Ex vivo expansion of limbal stem cells is affected by substrate properties","volume":"8","author":"Jones","year":"2012","journal-title":"Stem Cell Res."},{"key":"10.1016\/j.exer.2020.108181_bib77","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1002\/stem.1541","article-title":"Differentiation potential of limbal fibroblasts and bone marrow mesenchymal stem cells to corneal epithelial cells","volume":"32","author":"Katikireddy","year":"2014","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib78","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1159\/000417087","article-title":"Limbal autograft transplantation for chemical and thermal burns","volume":"18","author":"Kenyon","year":"1989","journal-title":"Dev. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib79","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.ajo.2008.01.009","article-title":"Sutureless amniotic membrane transplantation for partial limbal stem cell deficiency","volume":"145","author":"Kheirkhah","year":"2008","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib80","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1097\/ICO.0b013e31815873da","article-title":"Role of conjunctival inflammation in surgical outcome after amniotic membrane transplantation with or without fibrin glue for pterygium","volume":"27","author":"Kheirkhah","year":"2008","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2020.108181_bib81","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1001\/archopht.126.8.1059","article-title":"Temporary sutureless amniotic membrane patch for acute alkaline burns","volume":"126","author":"Kheirkhah","year":"2008","journal-title":"Arch. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib82","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1097\/01.ico.0000611432.24610.9b","article-title":"Minimal conjunctival limbal autograft for total limbal stem cell deficiency","volume":"27","author":"Kheirkhah","year":"2008","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2020.108181_bib83","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1097\/00003226-199509000-00006","article-title":"Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas","volume":"14","author":"Kim","year":"1995","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2020.108181_bib84","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1097\/ICU.0000000000000387","article-title":"Diagnosis of corneal limbal stem cell deficiency","volume":"28","author":"Kim","year":"2017","journal-title":"Curr. Opin. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib85","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1002\/stem.2921","article-title":"The role of nerve growth factor in maintaining proliferative capacity, colony-forming efficiency, and the limbal stem cell phenotype","volume":"37","author":"Kolli","year":"2019","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib86","doi-asserted-by":"crossref","first-page":"e7","DOI":"10.1097\/ICL.0b013e318255124f","article-title":"Placement of ProKera in the management of ocular manifestations of acute Stevens-Johnson syndrome in an outpatient","volume":"39","author":"Kolomeyer","year":"2013","journal-title":"Eye Contact Lens"},{"key":"10.1016\/j.exer.2020.108181_bib87","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1007\/s004170050012","article-title":"Cryopreserved human amniotic membrane for ocular surface reconstruction","volume":"238","author":"Kruse","year":"2000","journal-title":"Graefe\u2019s Arch. Clin. Exp. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib88","first-page":"289","article-title":"The importance of limbal stem cells for the therapy of chemical burns","volume":"2","author":"Kruse","year":"1993","journal-title":"Ger. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib89","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nature13426","article-title":"ABCB5 is a limbal stem cell gene required for corneal development and repair","volume":"511","author":"Ksander","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.exer.2020.108181_bib90","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1186\/1471-2164-11-526","article-title":"Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics","volume":"11","author":"Kulkarni","year":"2010","journal-title":"BMC Genom."},{"key":"10.1016\/j.exer.2020.108181_bib91","first-page":"1272","article-title":"Expression of nerve growth factor receptors on the ocular surface in healthy subjects and during manifestation of inflammatory diseases","volume":"39","author":"Lambiase","year":"1998","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib92","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1161\/CIRCRESAHA.108.188144","article-title":"Guidance of vascular development: lessons from the nervous system","volume":"104","author":"Larrivee","year":"2009","journal-title":"Circ. Res."},{"key":"10.1016\/j.exer.2020.108181_bib93","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1097\/PRS.0b013e3181eae781","article-title":"Scarless fetal wound healing: a basic science review","volume":"126","author":"Larson","year":"2010","journal-title":"Plast. Reconstr. Surg."},{"key":"10.1016\/j.exer.2020.108181_bib94","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.exer.2003.09.008","article-title":"Corneal epithelial stem cells at the limbus: looking at some old problems from a new angle","volume":"78","author":"Lavker","year":"2004","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2020.108181_bib95","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.jtos.2019.01.003","article-title":"The application of human amniotic membrane in the surgical management of limbal stem cell deficiency","volume":"17","author":"Le","year":"2019","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.exer.2020.108181_bib96","doi-asserted-by":"crossref","first-page":"3357","DOI":"10.1167\/iovs.11-9414","article-title":"Angiogenesis potential of human limbal stromal niche cells","volume":"53","author":"Li","year":"2012","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib97","doi-asserted-by":"crossref","first-page":"5686","DOI":"10.1167\/iovs.12-10300","article-title":"Mesenchymal stem cells derived from human limbal niche cells","volume":"53","author":"Li","year":"2012","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib98","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1126\/science.1180794","article-title":"Coexistence of quiescent and active adult stem cells in mammals","volume":"327","author":"Li","year":"2010","journal-title":"Science"},{"key":"10.1016\/j.exer.2020.108181_bib99","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1146\/annurev.cellbio.21.012704.131525","article-title":"Stem cell niche: structure and function","volume":"21","author":"Li","year":"2005","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"10.1016\/j.exer.2020.108181_bib100","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1002\/jcp.21345","article-title":"Reversal of myofibroblasts by amniotic membrane stromal extract","volume":"215","author":"Li","year":"2008","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.exer.2020.108181_bib101","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.exer.2005.06.022","article-title":"Amniotic membrane induces apoptosis of interferon-gamma activated macrophages in vitro","volume":"82","author":"Li","year":"2006","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2020.108181_bib102","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1001\/archophthalmol.2009.263","article-title":"Long-term outcomes of keratolimbal allograft for total limbal stem cell deficiency using combined immunosuppressive agents and correction of ocular surface deficits","volume":"127","author":"Liang","year":"2009","journal-title":"Arch. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib103","first-page":"2672","article-title":"In vitro preparation of human ocular surface epithelial cells for transplantation","volume":"34","author":"Lindberg","year":"1993","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib104","doi-asserted-by":"crossref","first-page":"24627","DOI":"10.1016\/S0021-9258(17)31438-2","article-title":"Characterization and chromosomal localization of the cornea-specific murine keratin gene Krt1.12","volume":"269","author":"Liu","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exer.2020.108181_bib105","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1586\/eop.10.63","article-title":"Update on amniotic membrane transplantation","volume":"5","author":"Liu","year":"2010","journal-title":"Expert.Rev.Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib106","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1111\/wrr.12754","article-title":"An open-label trial of cryopreserved human umbilical cord in the treatment of complex diabetic foot ulcers complicated by osteomyelitis","volume":"6","author":"Marston","year":"2019","journal-title":"Wound Repair Regen."},{"key":"10.1016\/j.exer.2020.108181_bib107","article-title":"Modulatory role of sensory innervation on hair follicle stem cell progeny during wound healing of the rat skin","volume":"7","author":"Martinez-Martinez","year":"2012","journal-title":"PloS One"},{"key":"10.1016\/j.exer.2020.108181_bib108","series-title":"Amniotic membrane transplantation for managing dry eye and neurotrophic keratitis","first-page":"13","volume":"1","author":"Mead","year":"2020"},{"key":"10.1016\/j.exer.2020.108181_bib109","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1016\/S0161-6420(03)00503-7","article-title":"Amniotic membrane transplantation with conjunctival limbal autograft for total limbal stem cell deficiency","volume":"110","author":"Meallet","year":"2003","journal-title":"Ophthalmology"},{"key":"10.1016\/j.exer.2020.108181_bib110","doi-asserted-by":"crossref","first-page":"879","DOI":"10.3390\/jfb3040879","article-title":"Extracellular matrix is an important component of limbal stem cell niche","volume":"3","author":"Mei","year":"2012","journal-title":"J. Funct. Biomater."},{"key":"10.1016\/j.exer.2020.108181_bib111","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1002\/stem.1582","article-title":"Frizzled 7 maintains the undifferentiated state of human limbal stem\/progenitor cells","volume":"32","author":"Mei","year":"2014","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib112","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/S0161-6420(00)00024-5","article-title":"Amniotic membrane transplantation for acute chemical or thermal burns","volume":"107","author":"Meller","year":"2000","journal-title":"Ophthalmology"},{"key":"10.1016\/j.exer.2020.108181_bib113","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1248\/bpb.21.167","article-title":"Gene expression of the two heavy chains and one light chain forming the inter-alpha-trypsin-inhibitor in human tissues","volume":"21","author":"Mizushima","year":"1998","journal-title":"Biol. Pharm. Bull."},{"key":"10.1016\/j.exer.2020.108181_bib114","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1016\/j.yexcr.2013.05.002","article-title":"Substrate elasticity as biomechanical modulator of tissue homeostatic parameters in corneal keratinocytes","volume":"319","author":"Moers","year":"2013","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.exer.2020.108181_bib115","first-page":"302","article-title":"Treatment of unilateral limbal stem cell deficiency syndrome by limbal autograft","volume":"22","author":"Moldovan","year":"1999","journal-title":"J. Fr. Ophtalmol."},{"key":"10.1016\/j.exer.2020.108181_bib116","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.jtos.2017.10.003","article-title":"Efficacy of self-retained cryopreserved amniotic membrane for treatment of neuropathic corneal pain","volume":"16","author":"Morkin","year":"2018","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.exer.2020.108181_bib117","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0014-4835(03)00050-2","article-title":"Corneal nerves: structure, contents and function","volume":"76","author":"Muller","year":"2003","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2020.108181_bib118","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1136\/bjo.2006.112656","article-title":"Age-related differences in the normal human cornea: a laser scanning in vivo confocal microscopy study","volume":"91","author":"Niederer","year":"2007","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib119","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s11357-011-9359-5","article-title":"The impact of age on the physical and cellular properties of the human limbal stem cell niche","volume":"35","author":"Notara","year":"2013","journal-title":"Age (Dordr)"},{"key":"10.1016\/j.exer.2020.108181_bib120","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1515\/bchm3.1990.371.2.1153","article-title":"Inter-alpha-trypsin inhibitor and pre-alpha-trypsin inhibitor in health and disease. Determination by immunoelectrophoresis and immunoblotting","volume":"371","author":"Odum","year":"1990","journal-title":"Biol. Chem. Hoppe Seyler"},{"key":"10.1016\/j.exer.2020.108181_bib121","doi-asserted-by":"crossref","DOI":"10.3390\/ijms18030606","article-title":"Keloid and hypertrophic scars are the result of chronic inflammation in the reticular dermis","volume":"18","author":"Ogawa","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib122","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/nature13465","article-title":"WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis","volume":"511","author":"Ouyang","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.exer.2020.108181_bib123","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1097\/AOG.0000000000001512","article-title":"Cryopreserved human umbilical cord for in utero myeloschisis repair","volume":"128","author":"Papanna","year":"2016","journal-title":"Obstet. Gynecol."},{"key":"10.1016\/j.exer.2020.108181_bib124","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1159\/000315020","article-title":"Midterm results of cultivated autologous and allogeneic limbal epithelial transplantation in limbal stem cell deficiency","volume":"45","author":"Pauklin","year":"2010","journal-title":"Dev. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib125","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1083\/jcb.145.4.769","article-title":"Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface","volume":"145","author":"Pellegrini","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.exer.2020.108181_bib126","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/S0140-6736(96)11188-0","article-title":"Long-term restoration of damaged corneal surface with autologous cultivated corneal epithelium","volume":"349","author":"Pellegrini","year":"1997","journal-title":"Lancet (London, England)"},{"key":"10.1016\/j.exer.2020.108181_bib127","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.it.2007.05.007","article-title":"The SDF-1-CXCR4 signaling pathway: a molecular hub modulating neo-angiogenesis","volume":"28","author":"Petit","year":"2007","journal-title":"Trends Immunol."},{"key":"10.1016\/j.exer.2020.108181_bib128","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1038\/ni813","article-title":"G-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4","volume":"3","author":"Petit","year":"2002","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.exer.2020.108181_bib129","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1002\/stem.2191","article-title":"Cell adhesion molecules and stem cell-niche-interactions in the limbal stem cell niche","volume":"34","author":"Polisetti","year":"2016","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib130","first-page":"319","article-title":"Efficacy of amniotic membrane patching for acute chemical and thermal ocular burns","volume":"90","author":"Prabhasawat","year":"2007","journal-title":"J. Med. Assoc. Thai."},{"key":"10.1016\/j.exer.2020.108181_bib131","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1177\/112067210301300602","article-title":"Ex vivo expansion of corneal limbal epithelial\/stem cells for corneal surface reconstruction","volume":"13","author":"Ramaesh","year":"2003","journal-title":"Eur. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib132","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1097\/00003226-199903000-00004","article-title":"Limbal autografting: comparison of results in the acute and chronic phases of ocular surface burns","volume":"18","author":"Rao","year":"1999","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2020.108181_bib133","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1038\/sj.leu.2403184","article-title":"Stem cell plasticity revisited: CXCR4-positive cells expressing mRNA for early muscle, liver and neural cells 'hide out' in the bone marrow","volume":"18","author":"Ratajczak","year":"2004","journal-title":"Leukemia"},{"key":"10.1016\/j.exer.2020.108181_bib134","doi-asserted-by":"crossref","first-page":"5125","DOI":"10.1167\/iovs.03-0628","article-title":"Different cell sizes in human limbal and central corneal basal epithelia measured by confocal microscopy and flow cytometry","volume":"44","author":"Romano","year":"2003","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib135","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.jtos.2016.06.003","article-title":"Contact lens-induced limbal stem cell deficiency","volume":"14","author":"Rossen","year":"2016","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.exer.2020.108181_bib136","doi-asserted-by":"crossref","first-page":"25674","DOI":"10.1074\/jbc.M501332200","article-title":"Characterization of complexes formed between TSG-6 and inter-alpha-inhibitor that act as intermediates in the covalent transfer of heavy chains onto hyaluronan","volume":"280","author":"Rugg","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exer.2020.108181_bib137","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.scr.2018.11.001","article-title":"Limbal epithelial stem cell activity and corneal epithelial cell cycle parameters in adult and aging mice","volume":"33","author":"Sagga","year":"2018","journal-title":"Stem Cell Res."},{"key":"10.1016\/j.exer.2020.108181_bib138","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj3150001","article-title":"The inter-a-inhibitor family: from structure to regulation","volume":"315","author":"Salier","year":"1996","journal-title":"Biochem. J."},{"key":"10.1016\/j.exer.2020.108181_bib139","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1242\/dev.01056","article-title":"PTX3 plays a key role in the organization of the cumulus oophorus extracellular matrix and in in vivo fertilization","volume":"131","author":"Salustri","year":"2004","journal-title":"Development"},{"key":"10.1016\/j.exer.2020.108181_bib140","doi-asserted-by":"crossref","first-page":"7661","DOI":"10.1021\/bi060106s","article-title":"Evidence for a two-step mechanism involved in the formation of covalent HC x TSG-6 complexes","volume":"45","author":"Sanggaard","year":"2006","journal-title":"Biochemistry"},{"key":"10.1016\/j.exer.2020.108181_bib141","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1136\/bjophthalmol-2011-301164","article-title":"Simple limbal epithelial transplantation (SLET): a novel surgical technique for the treatment of unilateral limbal stem cell deficiency","volume":"96","author":"Sangwan","year":"2012","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib142","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1136\/bjophthalmol-2011-300352","article-title":"Clinical outcomes of xeno-free autologous cultivated limbal epithelial transplantation: a 10-year study","volume":"95","author":"Sangwan","year":"2011","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib143","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4103\/0301-4738.33036","article-title":"Amniotic membrane transplantation: a review of current indications in the management of ophthalmic disorders","volume":"55","author":"Sangwan","year":"2007","journal-title":"Indian J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib144","first-page":"153","article-title":"New perspectives in ocular surface disorders. An Integrated Approach for Diagnosis and Management","volume":"49","author":"Sangwan","year":"2001","journal-title":"Indian J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib145","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.ajo.2005.03.022","article-title":"Survival analysis of conjunctival limbal grafts and amniotic membrane transplantation in eyes with total limbal stem cell deficiency","volume":"140","author":"Santos","year":"2005","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib146","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1083\/jcb.103.1.49","article-title":"Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells","volume":"103","author":"Schermer","year":"1986","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.exer.2020.108181_bib147","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/j.exer.2007.08.020","article-title":"Characterization of extracellular matrix components in the limbal epithelial stem cell compartment","volume":"85","author":"Schlotzer-Schrehardt","year":"2007","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2020.108181_bib148","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.ajo.2009.08.040","article-title":"Management of acute Stevens-Johnson syndrome and toxic epidermal necrolysis utilizing amniotic membrane and topical corticosteroids","volume":"149","author":"Shammas","year":"2010","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib149","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.2147\/OPTH.S181035","article-title":"Amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency","volume":"12","author":"Sharma","year":"2018","journal-title":"Clin. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib150","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1167\/iovs.10-5888","article-title":"Inhibition of angiogenesis by HC.HA, a complex of hyaluronan and the heavy chain of inter-alpha-inhibitor, purified from human amniotic membrane","volume":"52","author":"Shay","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib151","doi-asserted-by":"crossref","first-page":"265","DOI":"10.5966\/sctm.2013-0025","article-title":"Three-year outcomes of cultured limbal epithelial allografts in aniridia and Stevens-Johnson syndrome evaluated using the Clinical Outcome Assessment in Surgical Trials assessment tool","volume":"3","author":"Shortt","year":"2014","journal-title":"Stem Cells Transl Med"},{"key":"10.1016\/j.exer.2020.108181_bib152","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1634\/stemcells.2006-0580","article-title":"Characterization of the limbal epithelial stem cell niche: novel imaging techniques permit in vivo observation and targeted biopsy of limbal epithelial stem cells","volume":"25","author":"Shortt","year":"2007","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib153","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1097\/OPX.0000000000001132","article-title":"Case report: use of self-retained cryopreserved amniotic membrane in the treatment of acute chemical ocular burn","volume":"94","author":"Shupe","year":"2017","journal-title":"Optom. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib154","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1166\/jbt.2014.1180","article-title":"Structural and biological comparison of cryopreserved and fresh amniotic membrane tissues","volume":"4","author":"Tan","year":"2014","journal-title":"Journal of Biomaterials and Tissue Engineering"},{"key":"10.1016\/j.exer.2020.108181_bib155","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s13287-016-0342-z","article-title":"Donor age and long-term culture do not negatively influence the stem potential of limbal fibroblast-like stem cells","volume":"7","author":"Tomasello","year":"2016","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.exer.2020.108181_bib156","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1097\/ICO.0b013e318243fee3","article-title":"Triple-TEN\" in the treatment of acute ocular complications from toxic epidermal necrolysis","volume":"32","author":"Tomlins","year":"2013","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2020.108181_bib157","first-page":"987","article-title":"The role of NGF signaling in human limbal epithelium expanded by amniotic membrane culture","volume":"43","author":"Touhami","year":"2002","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib158","first-page":"721","article-title":"The limbal palisades of Vogt 1","volume":"89","author":"Townsend","year":"1991","journal-title":"Trans. Am. Ophthalmol. Soc."},{"key":"10.1016\/j.exer.2020.108181_bib159","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/S0002-9394(00)00746-7","article-title":"Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells(1)","volume":"130","author":"Tsai","year":"2000","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib160","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1097\/00003226-199509000-00001","article-title":"Effect of stromal inflammation on the outcome of limbal transplantation for corneal surface reconstruction","volume":"14","author":"Tsai","year":"1995","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2020.108181_bib161","article-title":"HC-HA\/PTX3 purified from amniotic membrane as novel regenerative matrix: insight into relationship between inflammation and regeneration","volume":"57","author":"Tseng","year":"2016","journal-title":"IOVS (Investig. Ophthalmol. Vis. Sci.)"},{"key":"10.1016\/j.exer.2020.108181_bib162","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1023\/A:1017995810755","article-title":"Amniotic membrane transplantation for ocular surface reconstruction","volume":"21","author":"Tseng","year":"2001","journal-title":"Biosci. Rep."},{"key":"10.1016\/j.exer.2020.108181_bib163","doi-asserted-by":"crossref","first-page":"841","DOI":"10.2174\/156652410793937796","article-title":"Critical appraisal of ex vivo expansion of human limbal epithelial stem cells","volume":"10","author":"Tseng","year":"2010","journal-title":"Curr. Mol. Med."},{"key":"10.1016\/j.exer.2020.108181_bib164","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jtos.2015.12.002","article-title":"Niche regulation of limbal epithelial stem cells: relationship between inflammation and regeneration","volume":"14","author":"Tseng","year":"2016","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.exer.2020.108181_bib165","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1001\/archopht.116.4.431","article-title":"Amniotic membrane transplantation with or without limbal allografts for corneal surface reconstruction in patients with limbal stem cell deficiency","volume":"116","author":"Tseng","year":"1998","journal-title":"Arch. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib166","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/eye.1989.22","article-title":"Concept and application of limbal stem cells","volume":"3","author":"Tseng","year":"1989","journal-title":"Eye"},{"key":"10.1016\/j.exer.2020.108181_bib167","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1167\/iovs.11-8438","article-title":"Dependence of corneal stem\/progenitor cells on ocular surface innervation","volume":"53","author":"Ueno","year":"2012","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib168","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1634\/stemcells.2005-0064","article-title":"Limbal epithelial side-population cells have stem cell-like properties, including quiescent state","volume":"24","author":"Umemoto","year":"2006","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib169","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ajo.2018.01.020","article-title":"Limbal stem cell deficiency-demography and underlying causes","volume":"188","author":"Vazirani","year":"2018","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib170","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1111\/jcmm.12061","article-title":"Cell adhesion and mechanical stimulation in the regulation of mesenchymal stem cell differentiation","volume":"17","author":"Wang","year":"2013","journal-title":"J. Cell Mol. Med."},{"key":"10.1016\/j.exer.2020.108181_bib171","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1097\/00003226-200404000-00013","article-title":"Clinicopathologic study of conjunctivochalasis","volume":"23","author":"Watanabe","year":"2004","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2020.108181_bib173","doi-asserted-by":"crossref","first-page":"7195","DOI":"10.1073\/pnas.1504177112","article-title":"Neural Hedgehog signaling maintains stem cell renewal in the sensory touch dome epithelium","volume":"112","author":"Xiao","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.exer.2020.108181_bib174","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1002\/stem.743","article-title":"Limbal epithelial stem\/progenitor cells attract stromal niche cells by SDF-1\/CXCR4 signaling to prevent differentiation","volume":"29","author":"Xie","year":"2011","journal-title":"Stem Cell."},{"key":"10.1016\/j.exer.2020.108181_bib175","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1167\/iovs.11-8441","article-title":"Isolation and expansion of human limbal stromal niche cells","volume":"53","author":"Xie","year":"2012","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib176","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcjo.2014.05.006","article-title":"Indications for amniotic membrane grafts used in Ontario from 2011 to 2012","volume":"49","author":"Yang","year":"2014","journal-title":"Can. J. Ophthalmol."},{"key":"10.1016\/j.exer.2020.108181_bib177","doi-asserted-by":"crossref","first-page":"4780","DOI":"10.1167\/iovs.06-0574","article-title":"Cytokeratin 15 can be used to identify the limbal phenotype in normal and diseased ocular surfaces","volume":"47","author":"Yoshida","year":"2006","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib178","doi-asserted-by":"crossref","first-page":"12433","DOI":"10.1074\/jbc.M112.342873","article-title":"Constitutive expression of inter-alpha-inhibitor (IalphaI) family proteins and tumor necrosis factor-stimulated gene-6 (TSG-6) by human amniotic membrane epithelial and stromal cells supporting formation of the heavy chain-hyaluronan (HC-HA) complex","volume":"287","author":"Zhang","year":"2012","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exer.2020.108181_bib179","doi-asserted-by":"crossref","first-page":"13531","DOI":"10.1074\/jbc.M113.525287","article-title":"Constitutive expression of pentraxin 3 (PTX3) protein by human amniotic membrane cells leads to formation of the heavy chain (HC)-hyaluronan (HA)-PTX3 complex","volume":"289","author":"Zhang","year":"2014","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exer.2020.108181_bib180","first-page":"469","article-title":"[Evolving concepts on limbal stem cells and their niche]","volume":"44","author":"Zheng","year":"2008","journal-title":"Zhonghua Yan Ke Za Zhi"},{"key":"10.1016\/j.exer.2020.108181_bib181","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1167\/iovs.61.5.62","article-title":"HC-HA\/PTX3 purified from human amniotic membrane reverts human corneal fibroblasts and myofibroblasts to keratocytes by activating BMP signaling","volume":"61","author":"Zhu","year":"2020","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2020.108181_bib182","doi-asserted-by":"crossref","first-page":"38079","DOI":"10.1074\/jbc.R300039200","article-title":"Inter-alpha-trypsin inhibitor, a covalent protein-glycosaminoglycan-protein complex","volume":"279","author":"Zhuo","year":"2004","journal-title":"J. Biol. Chem."}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483520304395?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483520304395?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T05:06:01Z","timestamp":1757999161000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483520304395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":181,"alternative-id":["S0014483520304395"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2020.108181","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Niche regulation of limbal epithelial stem cells: HC-HA\/PTX3 as surrogate matrix niche","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2020.108181","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"108181"}}