{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T04:04:47Z","timestamp":1773201887899,"version":"3.50.1"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2016YFC1101200"],"award-info":[{"award-number":["2016YFC1101200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1101200"],"award-info":[{"award-number":["2021YFA1101200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770926"],"award-info":[{"award-number":["81770926"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800842"],"award-info":[{"award-number":["81800842"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2022,6]]},"DOI":"10.1016\/j.exer.2022.108956","type":"journal-article","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T06:40:34Z","timestamp":1648708834000},"page":"108956","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["High-quality, large-scale, semi-thin, &amp; ultra-thin sections of the optic nerve in large animals: An optimized procedure"],"prefix":"10.1016","volume":"219","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8422-9061","authenticated-orcid":false,"given":"Mengyun","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3933-9039","authenticated-orcid":false,"given":"Shenjian","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PingPing","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhonghao","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhao","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiyang","family":"Mao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2741-8274","authenticated-orcid":false,"given":"Chen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuaishuai","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wencan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yikui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.exer.2022.108956_bib1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3389\/fncel.2016.00005","article-title":"Improved methods for fluorescence microscopy detection of macromolecules at the axon initial segment","volume":"10","author":"Alshammari","year":"2016","journal-title":"Front. Cell. Neurosci."},{"key":"10.1016\/j.exer.2022.108956_bib2","first-page":"741","article-title":"Xylazine: a new sedative-analgesic with predictable emetic properties in the cat","volume":"68","author":"Amend","year":"1973","journal-title":"Vet. Med. Small Anim. Clin. : VM, SAC"},{"key":"10.1016\/j.exer.2022.108956_bib3","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/S0161-6420(82)34769-7","article-title":"Optic nerve blindness following blunt forehead trauma","volume":"89","author":"Anderson","year":"1982","journal-title":"Ophthalmology"},{"key":"10.1016\/j.exer.2022.108956_bib4","doi-asserted-by":"crossref","DOI":"10.1117\/1.JMI.3.1.017002","article-title":"Active monitoring of formaldehyde diffusion into histological tissues with digital acoustic interferometry","volume":"3","author":"Bauer","year":"2016","journal-title":"J. Med. Imaging"},{"key":"10.1016\/j.exer.2022.108956_bib5","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0165-0270(87)80001-8","article-title":"Perfusion-fixation of the human brain for immunohistochemistry: comparison with immersion-fixation","volume":"19","author":"Beach","year":"1987","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.exer.2022.108956_bib6","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1212\/CON.0000000000000768","article-title":"Optic neuritis","volume":"25","author":"Bennett","year":"2019","journal-title":"Continuum"},{"key":"10.1016\/j.exer.2022.108956_bib7","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1289\/ehp.7542","article-title":"Meeting report: summary of IARC monographs on formaldehyde, 2-butoxyethanol, and 1-tert-butoxy-2-propanol","volume":"113","author":"Cogliano","year":"2005","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.exer.2022.108956_bib8","series-title":"Review of the Formaldehyde Assessment in the National Toxicology Program 12th Report on Carcinogens","year":"2014"},{"key":"10.1016\/j.exer.2022.108956_bib10","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1097\/00061198-200308000-00003","article-title":"Estimating normal optic nerve axon numbers in non-human primate eyes","volume":"12","author":"Cull","year":"2003","journal-title":"J. Glaucoma"},{"key":"10.1016\/j.exer.2022.108956_bib11","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1097\/PDM.0b013e3181b520f8","article-title":"Effects of formalin, methacarn, and fineFIX fixatives on RNA preservation","volume":"19","author":"Dotti","year":"2010","journal-title":"Diagn. Mol. Pathol. : the American journal of surgical pathology, part B"},{"key":"10.1016\/j.exer.2022.108956_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ncl.2016.08.003","article-title":"New treatments for nonarteritic anterior ischemic optic neuropathy","volume":"35","author":"Foroozan","year":"2017","journal-title":"Neurol. Clin."},{"key":"10.1016\/j.exer.2022.108956_bib13","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1167\/tvst.3.3.2","article-title":"The effect of age on optic nerve axon counts, SDOCT scan quality, and peripapillary retinal nerve fiber layer thickness measurements in rhesus monkeys","volume":"3","author":"Fortune","year":"2014","journal-title":"Transl Vis. Sci. Technol."},{"key":"10.1016\/j.exer.2022.108956_bib14","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1136\/bjo.86.2.233","article-title":"Quantification of histological changes after calibrated crush of the intraorbital optic nerve in rats","volume":"86","author":"Gellrich","year":"2002","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.exer.2022.108956_bib15","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1111\/j.1751-0813.2004.tb12712.x","article-title":"Comparison of the analgesic effects of xylazine in sheep via three different administration routes","volume":"82","author":"Grant","year":"2004","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.exer.2022.108956_bib16","doi-asserted-by":"crossref","first-page":"963","DOI":"10.3171\/jns.1981.55.6.0963","article-title":"Evidence for orbital deformation that may contribute to monocular blindness following minor frontal head trauma","volume":"55","author":"Gross","year":"1981","journal-title":"J. Neurosurg."},{"key":"10.1016\/j.exer.2022.108956_bib17","first-page":"1","article-title":"Penetration rates of osmium tetroxide with different fixation vehicles","volume":"2","author":"Hagstroem","year":"1960","journal-title":"Z Zellforch Microsk Anat Histochem"},{"key":"10.1016\/j.exer.2022.108956_bib18","doi-asserted-by":"crossref","first-page":"42","DOI":"10.2460\/javma.1974.164.01.42","article-title":"Clinical evaluation of xylazine as a chemical restraining agent, sedative, and analgesic in horses","volume":"164","author":"Hoffman","year":"1974","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"10.1016\/j.exer.2022.108956_bib19","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1177\/019459988309100313","article-title":"Incidence of eye injuries in facial fractures: an analysis of 727 cases","volume":"91","author":"Holt","year":"1983","journal-title":"Otolaryngol. Head Neck Surg."},{"key":"10.1016\/j.exer.2022.108956_bib20","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.jneumeth.2009.08.019","article-title":"Transcardial perfusion versus immersion fixation for assessment of peripheral nerve regeneration","volume":"184","author":"Kasukurthi","year":"2009","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.exer.2022.108956_bib21","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1017\/S1551929500057060","article-title":"Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: what they are and what they do","volume":"8","author":"Kiernan","year":"2000","journal-title":"Microscopy Today"},{"key":"10.1016\/j.exer.2022.108956_bib22","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.jneumeth.2015.06.019","article-title":"The use of a cerebral perfusion and immersion-fixation process for subsequent white matter dissection","volume":"253","author":"Latini","year":"2015","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.exer.2022.108956_bib23","doi-asserted-by":"crossref","first-page":"4436","DOI":"10.1093\/nar\/27.22.4436","article-title":"Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples","volume":"27","author":"Masuda","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.exer.2022.108956_bib24","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ajo.2017.04.012","article-title":"Reduced free communication of the subarachnoid space within the optic canal in the human","volume":"179","author":"Mikee","year":"2017","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.exer.2022.108956_bib25","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/S0091-679X(10)96020-2","article-title":"Electron microscopy of the mouse central nervous system","volume":"96","author":"M\u00f6bius","year":"2010","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.exer.2022.108956_bib26","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1212\/CON.0000000000000767","article-title":"Ischemic optic neuropathy","volume":"25","author":"Morrow","year":"2019","journal-title":"Continuum"},{"key":"10.1016\/j.exer.2022.108956_bib27","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1177\/23.2.1117127","article-title":"Rapid chemical dehydration of samples for electron microscopic examinations","volume":"23","author":"Muller","year":"1975","journal-title":"J. Histochem. Cytochem. : Off. J. Histochem. Soc."},{"key":"10.1016\/j.exer.2022.108956_bib28","first-page":"677","article-title":"Xylazine as a sedative-analgesic for dogs and cats","volume":"55","author":"Newkirk","year":"1974","journal-title":"Mod. Vet. Pract."},{"key":"10.1016\/j.exer.2022.108956_bib29","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/ejn.12447","article-title":"A protocol for concurrent high-quality immunohistochemical and biochemical analyses in adult mouse central nervous system","volume":"39","author":"Notter","year":"2014","journal-title":"Eur. J. Neurosci."},{"key":"10.1016\/j.exer.2022.108956_bib30","first-page":"1049","volume":"39","author":"Pathan","year":"2008"},{"key":"10.1016\/j.exer.2022.108956_bib31","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1369\/0022155416649579","article-title":"A buffered alcohol-based fixative for histomorphologic and molecular applications","volume":"64","author":"Perry","year":"2016","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.exer.2022.108956_bib32","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.2460\/ajvr.1979.40.08.1082","article-title":"Objective tests of analgesic drugs in ponies","volume":"40","author":"Pippi","year":"1979","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.exer.2022.108956_bib33","doi-asserted-by":"crossref","first-page":"711","DOI":"10.3390\/biom11050711","article-title":"The combination of paraformaldehyde and glutaraldehyde is a potential fixative for mitochondria","volume":"11","author":"Qin","year":"2021","journal-title":"Biomolecules"},{"key":"10.1016\/j.exer.2022.108956_bib34","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0306-4522(88)90231-X","article-title":"Axon diameter distributions across the monkey's optic nerve","volume":"27","author":"Reese","year":"1988","journal-title":"Neuroscience"},{"key":"10.1016\/j.exer.2022.108956_bib35","doi-asserted-by":"crossref","first-page":"139","DOI":"10.15252\/embj.201695709","article-title":"Glyoxal as an alternative fixative to formaldehyde in immunostaining and super\u2010resolution microscopy","volume":"37","author":"Richter","year":"2018","journal-title":"EMBO J."},{"key":"10.1016\/j.exer.2022.108956_bib36","first-page":"291","article-title":"Stability and autolysis of cortical neurons in post-mortem adult rat brains","volume":"1","author":"Sheleg","year":"2008","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"10.1016\/j.exer.2022.108956_bib37","article-title":"Fixation and fixatives: roles and functions\u2014a short review","volume":"7","author":"Singh","year":"2019","journal-title":"Dental J. Adv. Studies"},{"key":"10.1016\/j.exer.2022.108956_bib38","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/BF01102871","article-title":"Electron microscopic study on the medial defect at the apex of human cerebral arterial bifurcations","volume":"382","author":"Suzuki","year":"1979","journal-title":"Virchows Archiv - A Pathol. Anat. Histol."},{"key":"10.1016\/j.exer.2022.108956_bib39","first-page":"1","article-title":"The effect of methanol fixation on single-cell RNA sequencing data","volume":"22","author":"Wang","year":"2021","journal-title":"BMC Genom."},{"key":"10.1016\/j.exer.2022.108956_bib40","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1167\/iovs.09-5122","article-title":"Deformation of the early glaucomatous monkey optic nerve head connective tissue after acute IOP elevation in 3-D histomorphometric reconstructions","volume":"52","author":"Yang","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.108956_bib41","doi-asserted-by":"crossref","first-page":"119","DOI":"10.3389\/fncel.2020.00119","article-title":"Strategies to promote long-distance optic nerve regeneration","volume":"14","author":"Yang","year":"2020","journal-title":"Front. Cell. Neurosci."},{"key":"10.1016\/j.exer.2022.108956_bib42","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.tjo.2015.01.003","article-title":"Traumatic optic neuropathy-Clinical features and management issues","volume":"5","author":"Yu-Wai-Man","year":"2015","journal-title":"Taiwan J. Ophthalmology"},{"key":"10.1016\/j.exer.2022.108956_bib43","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.45881","article-title":"Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse","volume":"8","author":"Zhang","year":"2019","journal-title":"Elife"},{"key":"10.1016\/j.exer.2022.108956_bib44","doi-asserted-by":"crossref","first-page":"108652","DOI":"10.1016\/j.exer.2021.108652","article-title":"In vivo evaluation of outer retinal function and structure after retrobulbar optic nerve crush by lateral orbitotomy in goats","volume":"209","author":"Zhang","year":"2021","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.108956_bib45","doi-asserted-by":"crossref","first-page":"108117","DOI":"10.1016\/j.exer.2020.108117","article-title":"In vivo evaluation of retinal ganglion cells and optic nerve's integrity in large animals by multi-modality analysis","volume":"197","author":"Zhang","year":"2020","journal-title":"Exp. Eye Res."}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483522000379?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483522000379?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T05:33:18Z","timestamp":1762147998000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483522000379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":44,"alternative-id":["S0014483522000379"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2022.108956","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High-quality, large-scale, semi-thin, & ultra-thin sections of the optic nerve in large animals: An optimized procedure","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2022.108956","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"108956"}}